LOCUS       RC220597                8958 bp    DNA     circular  SYN 
DEFINITION  NPC1L1 (Myc-DDK-tagged)-Human NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (NPC1L1), transcript variant 1
ACCESSION   NM_013389
VERSION     NM_013389.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5115..5206 
                     /label="Myc-DDK"  
     misc_feature    5236..5821 
                     /label="PolyA signal"  
     misc_feature    5293..5312 
                     /label="Restriction Enzyme"  
     misc_feature    6097..6769 
                     /label="ColE1"  
     misc_feature    7199..7993 
                     /label="Kan/Neo"  
     misc_feature    8028..8330 
                     /label="SV40 ori"  
     misc_feature    8416..8893 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5097..5103 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5097 
                     /label="NPC1L1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGGCC GGCCTGAGGG GCTGGCTGCT GTGGGCCCTG CTCCTGCGCT 
     1081 TGGCCCAGAG TGAGCCTTAC ACAACCATCC ACCAGCCTGG CTACTGCGCC TTCTATGACG 
     1141 AATGTGGGAA GAACCCAGAG CTGTCTGGAA GCCTCATGAC ACTCTCCAAC GTGTCCTGCC 
     1201 TGTCCAACAC GCCGGCCCGC AAGATCACAG GTGATCACCT GATCCTATTA CAGAAGATCT 
     1261 GCCCCCGCCT CTACACCGGC CCCAACACCC AAGCCTGCTG CTCCGCCAAG CAGCTGGTAT 
     1321 CACTGGAAGC GAGTCTGTCG ATCACCAAGG CCCTCCTCAC CCGCTGCCCA GCCTGCTCTG 
     1381 ACAATTTTGT GAACCTGCAC TGCCACAACA CGTGCAGCCC CAATCAGAGC CTCTTCATCA 
     1441 ATGTGACCCG CGTGGCCCAG CTAGGGGCTG GACAACTCCC AGCTGTGGTG GCCTATGAGG 
     1501 CCTTCTACCA GCATAGCTTT GCCGAGCAGA GCTATGACTC CTGCAGCCGT GTGCGCGTCC 
     1561 CTGCAGCTGC CACGCTGGCT GTGGGCACCA TGTGTGGCGT GTATGGCTCT GCCCTTTGCA 
     1621 ATGCCCAGCG CTGGCTCAAC TTCCAGGGAG ACACAGGCAA TGGTCTGGCC CCACTGGACA 
     1681 TCACCTTCCA CCTCTTGGAG CCTGGCCAGG CCGTGGGGAG TGGGATTCAG CCTCTGAATG 
     1741 AGGGGGTTGC ACGTTGCAAT GAGTCCCAAG GTGACGACGT GGCGACCTGC TCCTGCCAAG 
     1801 ACTGTGCTGC ATCCTGTCCT GCCATAGCCC GCCCCCAGGC CCTCGACTCC ACCTTCTACC 
     1861 TGGGCCAGAT GCCGGGCAGT CTGGTCCTCA TCATCATCCT CTGCTCTGTC TTCGCTGTGG 
     1921 TCACCATCCT GCTTGTGGGA TTCCGTGTGG CCCCCGCCAG GGACAAAAGC AAGATGGTGG 
     1981 ACCCCAAGAA GGGCACCAGC CTCTCTGACA AGCTCAGCTT CTCCACCCAC ACCCTCCTTG 
     2041 GCCAGTTCTT CCAGGGCTGG GGCACGTGGG TGGCTTCGTG GCCTCTGACC ATCTTGGTGC 
     2101 TATCTGTCAT CCCGGTGGTG GCCTTGGCAG CGGGCCTGGT CTTTACAGAA CTCACTACGG 
     2161 ACCCCGTGGA GCTGTGGTCG GCCCCCAACA GCCAAGCCCG GAGTGAGAAA GCTTTCCATG 
     2221 ACCAGCATTT CGGCCCCTTC TTCCGAACCA ACCAGGTGAT CCTGACGGCT CCTAACCGGT 
     2281 CCAGCTACAG GTATGACTCT CTGCTGCTGG GGCCCAAGAA CTTCAGCGGA ATCCTGGACC 
     2341 TGGACTTGCT GCTGGAGCTG CTAGAGCTGC AGGAGAGGCT GCGGCACCTC CAGGTATGGT 
     2401 CGCCCGAAGC ACAGCGCAAC ATCTCCCTGC AGGACATCTG CTACGCCCCC CTCAATCCGG 
     2461 ACAATACCAG TCTCTACGAC TGCTGCATCA ACAGCCTCCT GCAGTATTTC CAGAACAACC 
     2521 GCACGCTCCT GCTGCTCACA GCCAACCAGA CACTGATGGG GCAGACCTCC CAAGTCGACT 
     2581 GGAAGGACCA TTTTCTGTAC TGTGCCAATG CCCCGCTCAC CTTCAAGGAT GGCACAGCCC 
     2641 TGGCCCTGAG CTGCATGGCT GACTACGGGG CCCCTGTCTT CCCCTTCCTT GCCATTGGGG 
     2701 GGTACAAAGG AAAGGACTAT TCTGAGGCAG AGGCCCTGAT CATGACGTTC TCCCTCAACA 
     2761 ATTACCCTGC CGGGGACCCC CGTCTGGCCC AGGCCAAGCT GTGGGAGGAG GCCTTCTTAG 
     2821 AGGAAATGCG AGCCTTCCAG CGTCGGATGG CTGGCATGTT CCAGGTCACG TTCATGGCTG 
     2881 AGCGCTCTCT GGAAGACGAG ATCAATCGCA CCACAGCTGA AGACCTGCCC ATCTTTGCCA 
     2941 CCAGCTACAT TGTCATATTC CTGTACATCT CTCTGGCCCT GGGCAGCTAT TCCAGCTGGA 
     3001 GCCGAGTGAT GGTGGACTCC AAGGCCACGC TGGGCCTCGG CGGGGTGGCC GTGGTCCTGG 
     3061 GAGCAGTCAT GGCTGCCATG GGCTTCTTCT CCTACTTGGG TATCCGCTCC TCCCTGGTCA 
     3121 TCCTGCAAGT GGTTCCTTTC CTGGTGCTGT CCGTGGGGGC TGATAACATC TTCATCTTTG 
     3181 TTCTCGAGTA CCAGAGGCTG CCCCGGAGGC CTGGGGAGCC ACGAGAGGTC CACATTGGGC 
     3241 GAGCCCTAGG CAGGGTGGCT CCCAGCATGC TGTTGTGCAG CCTCTCTGAG GCCATCTGCT 
     3301 TCTTCCTAGG GGCCCTGACC CCCATGCCAG CTGTGCGGAC CTTTGCCCTG ACCTCTGGCC 
     3361 TTGCAGTGAT CCTTGACTTC CTCCTGCAGA TGTCAGCCTT TGTGGCCCTG CTCTCCCTGG 
     3421 ACAGCAAGAG GCAGGAGGCC TCCCGGTTGG ACGTCTGCTG CTGTGTCAAG CCCCAGGAGC 
     3481 TGCCCCCGCC TGGCCAGGGA GAGGGGCTCC TGCTTGGCTT CTTCCAAAAG GCTTATGCCC 
     3541 CCTTCCTGCT GCACTGGATC ACTCGAGGTG TTGTGCTGCT GCTGTTTCTC GCCCTGTTCG 
     3601 GAGTGAGCCT CTACTCCATG TGCCACATCA GCGTGGGACT GGACCAGGAG CTGGCCCTGC 
     3661 CCAAGGACTC GTACCTGCTT GACTATTTCC TCTTTCTGAA CCGCTACTTC GAGGTGGGGG 
     3721 CCCCGGTGTA CTTTGTTACC ACCTTGGGCT ACAACTTCTC CAGCGAGGCT GGGATGAATG 
     3781 CCATCTGCTC CAGTGCAGGC TGCAACAACT TCTCCTTCAC CCAGAAGATC CAGTATGCCA 
     3841 CAGAGTTCCC TGAGCAGTCT TACCTGGCCA TCCCTGCCTC CTCCTGGGTG GATGACTTCA 
     3901 TTGACTGGCT GACCCCGTCC TCCTGCTGCC GCCTTTATAT ATCTGGCCCC AATAAGGACA 
     3961 AGTTCTGCCC CTCGACCGTC AACTCTCTGA ACTGCCTAAA GAACTGCATG AGCATCACGA 
     4021 TGGGCTCTGT GAGGCCCTCG GTGGAGCAGT TCCATAAGTA TCTTCCCTGG TTCCTGAACG 
     4081 ACCGGCCCAA CATCAAATGT CCCAAAGGCG GCCTGGCAGC ATACAGCACC TCTGTGAACT 
     4141 TGACTTCAGA TGGCCAGGTT TTAGACACAG TTGCCATTCT GTCACCCAGG CTGGAGTACA 
     4201 GTGGCACAAT CTCGGCTCAC TGCAACCTCT ACCTCCTGGA TTCAACCTCC AGGTTCATGG 
     4261 CCTATCACAA GCCCCTGAAA AACTCACAGG ATTACACAGA AGCTCTGCGG GCAGCTCGAG 
     4321 AGCTGGCAGC CAACATCACT GCTGACCTGC GGAAAGTGCC TGGAACAGAC CCGGCTTTTG 
     4381 AGGTCTTCCC CTACACGATC ACCAATGTGT TTTATGAGCA GTACCTGACC ATCCTCCCTG 
     4441 AGGGGCTCTT CATGCTCAGC CTCTGCCTTG TGCCCACCTT CGCTGTCTCC TGCCTCCTGC 
     4501 TGGGCCTGGA CCTGCGCTCC GGCCTCCTCA ACCTGCTCTC CATTGTCATG ATCCTCGTGG 
     4561 ACACTGTCGG CTTCATGGCC CTGTGGGGCA TCAGTTACAA TGCTGTGTCC CTCATCAACC 
     4621 TGGTCTCGGC GGTGGGCATG TCTGTGGAGT TTGTGTCCCA CATTACCCGC TCCTTTGCCA 
     4681 TCAGCACCAA GCCCACCTGG CTGGAGAGGG CCAAAGAGGC CACCATCTCT ATGGGAAGTG 
     4741 CGGTGTTTGC AGGTGTGGCC ATGACCAACC TGCCTGGCAT CCTTGTCCTG GGCCTCGCCA 
     4801 AGGCCCAGCT CATTCAGATC TTCTTCTTCC GCCTCAACCT CCTGATCACT CTGCTGGGCC 
     4861 TGCTGCATGG CTTGGTCTTC CTGCCCGTCA TCCTCAGCTA CGTGGGGCCT GACGTTAACC 
     4921 CGGCTCTGGC ACTGGAGCAG AAGCGGGCTG AGGAGGCGGT GGCAGCAGTC ATGGTGGCCT 
     4981 CTTGCCCAAA TCACCCCTCC CGAGTCTCCA CAGCTGACAA CATCTATGTC AACCACAGCT 
     5041 TTGAAGGTTC TATCAAAGGT GCTGGTGCCA TCAGCAACTT CTTGCCCAAC AATGGGCGGC 
     5101 AGTTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5161 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5221 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5281 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5341 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5401 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5461 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5521 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5581 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5641 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5701 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5761 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5821 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5881 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5941 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6001 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6061 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6121 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6181 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6241 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6301 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6361 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6421 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6481 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6541 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6601 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6661 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6721 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6781 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6841 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6901 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6961 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7021 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7081 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7141 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7201 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7261 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7321 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7381 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7441 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7501 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7561 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7621 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7681 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7741 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7801 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7861 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7921 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7981 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8041 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8101 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8161 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8221 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8281 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8341 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8401 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8461 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8521 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8581 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8641 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8701 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8761 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8821 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8881 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8941 AAATTTAACG CGAATTTT
//