LOCUS       RC220578                8634 bp    DNA     circular  SYN 
DEFINITION  CNGB1 (Myc-DDK-tagged)-Human cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 1
ACCESSION   NM_001297
VERSION     NM_001297.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4791..4882 
                     /label="Myc-DDK"  
     misc_feature    4912..5497 
                     /label="PolyA signal"  
     misc_feature    4969..4988 
                     /label="Restriction Enzyme"  
     misc_feature    5773..6445 
                     /label="ColE1"  
     misc_feature    6875..7669 
                     /label="Kan/Neo"  
     misc_feature    7704..8006 
                     /label="SV40 ori"  
     misc_feature    8092..8569 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4773..4779 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4773 
                     /label="CNGB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGGGCTGG GTCCAGAGGG TGCTGCCTCA GCCCCCAGGG ACCCCTCGGA 
     1081 AGACCAAGAT GCAGGAGGAA GAGGAAGTGG AACCAGAGCC AGAGATGGAG GCGGAGGTGG 
     1141 AACCAGAACC GAATCCTGAG GAGGCCGAGA CAGAGTCCGA GTCCATGCCC CCCGAAGAGT 
     1201 CATTCAAGGA GGAGGAAGTG GCTGTGGCAG ACCCAAGCCC TCAGGAGACC AAGGAGGCTG 
     1261 CCCTTACTTC CACCATATCC CTCCGGGCCC AGGGCGCTGA GATTTCTGAA ATGAATAGTC 
     1321 CCAGCCGCAG GGTACTGACC TGGCTCATGA AGGGCGTAGA GAAGGTGATC CCGCAGCCTG 
     1381 TTCACAGCAT CACGGAGGAC CCGGCTCAGA TCCTGGGGCA TGGCAGCACT GGGGACACAG 
     1441 GGTGCACAGA TGAACCCAAT GAGGCCCTTG AGGCCCAAGA CACTAGGCCT GGGCTGCGGC 
     1501 TGCTTCTGTG GCTGGAGCAG AATCTGGAAA GAGTGCTTCC TCAGCCCCCC AAATCCTCTG 
     1561 AGGTCTGGAG AGATGAGCCT GCAGTTGCTA CAGGTGCTGC CTCAGACCCA GCGCCTCCAG 
     1621 GACGCCCCCA GGAAATGGGG CCCAAGCTGC AGGCCCGGGA GACCCCCTCC CTGCCCACAC 
     1681 CCATCCCCCT GCAGCCCAAG GAGGAACCCA AGGAGGCACC AGCTCCAGAG CCCCAGCCCG 
     1741 GCTCCCAGGC CCAGACCTCC TCCCTGCCAC CAACCAGGGA CCCTGCCAGG CTGGTGGCAT 
     1801 GGGTCCTGCA CAGGCTGGAG ATGGCCTTGC CGCAGCCAGT GCTACATGGG AAAATAGGGG 
     1861 AACAGGAGCC TGACTCCCCT GGGATATGTG ATGTGCAGAC CATCAGCATC CTTCCTGGAG 
     1921 GACAAGTGGA GCCTGACCTT GTCCTAGAGG AGGTTGAACC GCCCTGGGAG GATGCCCACC 
     1981 AGGATGTCAG TACCAGCCCA CAGGGTACAG AGGTGGTTCC AGCTTATGAA GAAGAGAACA 
     2041 AAGCTGTGGA GAAGATGCCC AGAGAGCTGT CCCGGATTGA AGAGGAGAAA GAAGATGAGG 
     2101 AGGAGGAAGA GGAAGAGGAG GAGGAGGAGG AAGAGGAGGA GGTGACTGAG GTGCTGCTGG 
     2161 ATAGCTGTGT GGTGTCGCAG GTGGGCGTGG GCCAGAGTGA AGAAGACGGG ACCCGGCCCC 
     2221 AGAGCACTTC AGATCAGAAG CTGTGGGAGG AAGTTGGGGA GGAGGCCAAG AAGGAGGCTG 
     2281 AAGAGAAGGC CAAGGAGGAG GCCGAGGAGG TGGCTGAAGA GGAGGCTGAA AAGGAGCCCC 
     2341 AGGACTGGGC GGAGACCAAG GAGGAGCCTG AGGCTGAGGC CGAGGCTGCC AGTTCAGGAG 
     2401 TGCCTGCCAC GAAACAGCAC CCAGAAGTGC AGGTGGAAGA TACTGATGCT GATAGCTGCC 
     2461 CCCTCATGGC AGAAGAGAAT CCACCCTCAA CCGTGTTGCC GCCACCGTCT CCTGCCAAAT 
     2521 CAGACACCCT TATAGTCCCA AGCTCAGCCT CGGGGACACA CAGGAAGAAG CTGCCCTCTG 
     2581 AGGATGATGA GGCTGAAGAG CTCAAGGCGT TGTCACCAGC AGAGTCCCCA GTGGTTGCCT 
     2641 GGTCTGACCC CACCACCCCG AAGGACACTG ATGGCCAGGA CCGTGCGGCC TCCACGGCCA 
     2701 GCACAAATAG CGCCATCATC AACGACCGGC TCCAGGAGCT GGTGAAGCTC TTCAAGGAGC 
     2761 GGACAGAGAA AGTGAAGGAG AAACTCATTG ACCCTGACGT CACCTCTGAT GAGGAGAGCC 
     2821 CCAAGCCCTC CCCAGCCAAG AAAGCCCCAG AGCCAGCTCC AGACACAAAG CCCGCTGAAG 
     2881 CCGAGCCAGT GGAAGAGGAG CACTATTGCG ACATGCTCTG CTGCAAGTTC AAACACCGCC 
     2941 CCTGGAAGAA GTACCAGTTT CCCCAGAGCA TTGACCCGCT GACCAACCTG ATGTATGTCC 
     3001 TATGGCTGTT CTTCGTGGTG ATGGCCTGGA ATTGGAACTG TTGGCTGATT CCCGTGCGCT 
     3061 GGGCCTTCCC CTACCAGACC CCGGACAACA TCCACCACTG GCTGCTGATG GATTACCTAT 
     3121 GCGACCTCAT CTACTTCCTG GACATCACCG TGTTCCAGAC ACGCCTGCAG TTTGTCAGAG 
     3181 GCGGGGACAT CATTACGGAC AAAAAGGACA TGCGAAATAA CTACCTGAAG TCTCGCCGCT 
     3241 TCAAGATGGA CCTGCTCAGC CTCCTGCCCT TGGATTTTCT CTATTTGAAA GTCGGTGTGA 
     3301 ACCCCCTCCT CCGCCTGCCC CGCTGTTTAA AGTACATGGC CTTCTTCGAG TTTAACAGCC 
     3361 GCCTGGAATC CATCCTCAGC AAAGCCTACG TGTACAGGGT CATCAGGACC ACAGCCTACC 
     3421 TTCTCTACAG CCTGCATTTG AATTCCTGTC TTTATTACTG GGCATCGGCC TATCAGGGCC 
     3481 TCGGCTCCAC TCACTGGGTT TACGATGGCG TGGGAAACAG TTATATTCGC TGTTACTACT 
     3541 TTGCTGTGAA GACCCTCATC ACCATCGGGG GGCTGCCTGA CCCCAAGACA CTCTTTGAAA 
     3601 TTGTCTTCCA GCTGCTGAAT TATTTCACGG GCGTCTTTGC TTTCTCTGTG ATGATCGGAC 
     3661 AGATGAGAGA TGTGGTAGGG GCCGCCACCG CCGGACAGAC CTACTACCGC AGCTGCATGG 
     3721 ACAGCACGGT GAAGTACATG AATTTCTACA AGATCCCCAA GTCCGTGCAG AACCGCGTCA 
     3781 AGACCTGGTA CGAGTACACC TGGCACTCGC AAGGCATGCT GGATGAGTCA GAGCTGATGG 
     3841 TGCAGCTTCC AGACAAGATG CGGCTGGACC TCGCCATCGA CGTGAACTAC AACATCGTTA 
     3901 GCAAAGTCGC ACTCTTTCAG GGCTGTGACC GGCAGATGAT CTTTGACATG CTGAAGAGGC 
     3961 TTCGCTCTGT TGTCTACCTG CCCAACGACT ATGTGTGCAA GAAGGGGGAG ATCGGCCGTG 
     4021 AGATGTACAT CATCCAGGCA GGGCAAGTGC AGGTCTTGGG CGGCCCTGAT GGGAAATCTG 
     4081 TGCTGGTGAC GCTGAAAGCT GGATCTGTGT TTGGAGAAAT AAGCTTGCTG GCTGTTGGGG 
     4141 GCGGGAACCG GCGCACGGCC AACGTGGTGG CGCACGGGTT TACCAACCTC TTCATCCTGG 
     4201 ATAAGAAGGA CCTGAATGAG ATTTTGGTGC ATTATCCTGA GTCTCAGAAG TTACTCCGGA 
     4261 AGAAAGCCAG GCGCATGCTG AGAAGCAACA ATAAGCCCAA GGAGGAGAAG AGCGTGCTGA 
     4321 TCCTTCCACC CCGGGCGGGC ACCCCAAAGC TCTTCAACGC TGCCCTCGCT ATGACAGGAA 
     4381 AGATGGGTGG CAAGGGGGCA AAAGGCGGCA AACTTGCTCA CCTCCGGGCC CGGCTCAAAG 
     4441 AACTGGCCGC GCTGGAGGCG GCTGCAAAGC AGCAAGAGTT GGTGGAACAG GCCAAGAGCT 
     4501 CGCAAGACGT CAAGGGAGAG GAAGGCTCCG CCGCCCCAGA CCAGCACACG CACCCAAAGG 
     4561 AGGCCGCCAC CGACCCACCC GCGCCCCGGA CGCCCCCCGA GCCCCCGGGG TCTCCACCGA 
     4621 GCTCTCCACC GCCTGCCTCC CTTGGGAGGC CGGAGGGAGA GGAGGAGGGG CCGGCCGAGC 
     4681 CCGAAGAGCA CTCGGTGAGG ATCTGCATGA GCCCGGGCCC GGAGCCGGGA GAGCAGATCC 
     4741 TGTCGGTGAA GATGCCGGAG GAAAGGGAGG AGAAGGCGGA GACGCGTACG CGGCCGCTCG 
     4801 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4861 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4921 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4981 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5041 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5101 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5161 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5221 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5281 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5341 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5401 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5461 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5521 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5581 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5641 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5701 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5761 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5821 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5881 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5941 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6001 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6061 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6121 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6181 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6241 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6301 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6361 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6421 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6481 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6541 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6601 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6661 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6721 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6781 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6841 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6901 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6961 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7021 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7081 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7141 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7201 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7261 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7321 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7381 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7441 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7501 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7561 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7621 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7681 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7741 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7801 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7861 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8041 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8101 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8161 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8221 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8281 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8341 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8401 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8461 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8521 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8581 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//