LOCUS       RC220575                8571 bp    DNA     circular  SYN 
DEFINITION  DMD (Myc-DDK-tagged)-Human dystrophin (DMD), transcript variant D140ab
ACCESSION   NM_004022
VERSION     NM_004022.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4728..4819 
                     /label="Myc-DDK"  
     misc_feature    4849..5434 
                     /label="PolyA signal"  
     misc_feature    4906..4925 
                     /label="Restriction Enzyme"  
     misc_feature    5710..6382 
                     /label="ColE1"  
     misc_feature    6812..7606 
                     /label="Kan/Neo"  
     misc_feature    7641..7943 
                     /label="SV40 ori"  
     misc_feature    8029..8506 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4710..4716 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4710 
                     /label="DMD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCATCTTCC TTGATGTTGG AGGTACCTGC TCTGGCAGAT TTCAACCGGG 
     1081 CTTGGACAGA ACTTACCGAC TGGCTTTCTC TGCTTGATCA AGTTATAAAA TCACAGAGGG 
     1141 TGATGGTGGG TGACCTTGAG GATATCAACG AGATGATCAT CAAGCAGAAG GCAACAATGC 
     1201 AGGATTTGGA ACAGAGGCGT CCCCAGTTGG AAGAACTCAT TACCGCTGCC CAAAATTTGA 
     1261 AAAACAAGAC CAGCAATCAA GAGGCTAGAA CAATCATTAC GGATCGAATT GAAAGAATTC 
     1321 AGAATCAGTG GGATGAAGTA CAAGAACACC TTCAGAACCG GAGGCAACAG TTGAATGAAA 
     1381 TGTTAAAGGA TTCAACACAA TGGCTGGAAG CTAAGGAAGA AGCTGAGCAG GTCTTAGGAC 
     1441 AGGCCAGAGC CAAGCTTGAG TCATGGAAGG AGGGTCCCTA TACAGTAGAT GCAATCCAAA 
     1501 AGAAAATCAC AGAAACCAAG CAGTTGGCCA AAGACCTCCG CCAGTGGCAG ACAAATGTAG 
     1561 ATGTGGCAAA TGACTTGGCC CTGAAACTTC TCCGGGATTA TTCTGCAGAT GATACCAGAA 
     1621 AAGTCCACAT GATAACAGAG AATATCAATG CCTCTTGGAG AAGCATTCAT AAAAGGGTGA 
     1681 GTGAGCGAGA GGCTGCTTTG GAAGAAACTC ATAGATTACT GCAACAGTTC CCCCTGGACC 
     1741 TGGAAAAGTT TCTTGCCTGG CTTACAGAAG CTGAAACAAC TGCCAATGTC CTACAGGATG 
     1801 CTACCCGTAA GGAAAGGCTC CTAGAAGACT CCAAGGGAGT AAAAGAGCTG ATGAAACAAT 
     1861 GGCAAGACCT CCAAGGTGAA ATTGAAGCTC ACACAGATGT TTATCACAAC CTGGATGAAA 
     1921 ACAGCCAAAA AATCCTGAGA TCCCTGGAAG GTTCCGATGA TGCAGTCCTG TTACAAAGAC 
     1981 GTTTGGATAA CATGAACTTC AAGTGGAGTG AACTTCGGAA AAAGTCTCTC AACATTAGGT 
     2041 CCCATTTGGA AGCCAGTTCT GACCAGTGGA AGCGTCTGCA CCTTTCTCTG CAGGAACTTC 
     2101 TGGTGTGGCT ACAGCTGAAA GATGATGAAT TAAGCCGGCA GGCACCTATT GGAGGCGACT 
     2161 TTCCAGCAGT TCAGAAGCAG AACGATGTAC ATAGGGCCTT CAAGAGGGAA TTGAAAACTA 
     2221 AAGAACCTGT AATCATGAGT ACTCTTGAGA CTGTACGAAT ATTTCTGACA GAGCAGCCTT 
     2281 TGGAAGGACT AGAGAAACTC TACCAGGAGC CCAGAGAGCT GCCTCCTGAG GAGAGAGCCC 
     2341 AGAATGTCAC TCGGCTTCTA CGAAAGCAGG CTGAGGAGGT CAATACTGAG TGGGAAAAAT 
     2401 TGAACCTGCA CTCCGCTGAC TGGCAGAGAA AAATAGATGA GACCCTTGAA AGACTCCAGG 
     2461 AACTTCAAGA GGCCACGGAT GAGCTGGACC TCAAGCTGCG CCAAGCTGAG GTGATCAAGG 
     2521 GATCCTGGCA GCCCGTGGGC GATCTCCTCA TTGACTCTCT CCAAGATCAC CTCGAGAAAG 
     2581 TCAAGGCACT TCGAGGAGAA ATTGCGCCTC TGAAAGAGAA CGTGAGCCAC GTCAATGACC 
     2641 TTGCTCGCCA GCTTACCACT TTGGGCATTC AGCTCTCACC GTATAACCTC AGCACTCTGG 
     2701 AAGACCTGAA CACCAGATGG AAGCTTCTGC AGGTGGCCGT CGAGGACCGA GTCAGGCAGC 
     2761 TGCATGAAGC CCACAGGGAC TTTGGTCCAG CATCTCAGCA CTTTCTTTCC ACGTCTGTCC 
     2821 AGGGTCCCTG GGAGAGAGCC ATCTCGCCAA ACAAAGTGCC CTACTATATC AACCACGAGA 
     2881 CTCAAACAAC TTGCTGGGAC CATCCCAAAA TGACAGAGCT CTACCAGTCT TTAGCTGACC 
     2941 TGAATAATGT CAGATTCTCA GCTTATAGGA CTGCCATGAA ACTCCGAAGA CTGCAGAAGG 
     3001 CCCTTTGCTT GGATCTCTTG AGCCTGTCAG CTGCATGTGA TGCCTTGGAC CAGCACAACC 
     3061 TCAAGCAAAA TGACCAGCCC ATGGATATCC TGCAGATTAT TAATTGTTTG ACCACTATTT 
     3121 ATGACCGCCT GGAGCAAGAG CACAACAATT TGGTCAACGT CCCTCTCTGC GTGGATATGT 
     3181 GTCTGAACTG GCTGCTGAAT GTTTATGATA CGGGACGAAC AGGGAGGATC CGTGTCCTGT 
     3241 CTTTTAAAAC TGGCATCATT TCCCTGTGTA AAGCACATTT GGAAGACAAG TACAGATACC 
     3301 TTTTCAAGCA AGTGGCAAGT TCAACAGGAT TTTGTGACCA GCGCAGGCTG GGCCTCCTTC 
     3361 TGCATGATTC TATCCAAATT CCAAGACAGT TGGGTGAAGT TGCATCCTTT GGGGGCAGTA 
     3421 ACATTGAGCC AAGTGTCCGG AGCTGCTTCC AATTTGCTAA TAATAAGCCA GAGATCGAAG 
     3481 CGGCCCTCTT CCTAGACTGG ATGAGACTGG AACCCCAGTC CATGGTGTGG CTGCCCGTCC 
     3541 TGCACAGAGT GGCTGCTGCA GAAACTGCCA AGCATCAGGC CAAATGTAAC ATCTGCAAAG 
     3601 AGTGTCCAAT CATTGGATTC AGGTACAGGA GTCTAAAGCA CTTTAATTAT GACATCTGCC 
     3661 AAAGCTGCTT TTTTTCTGGT CGAGTTGCAA AAGGCCATAA AATGCACTAT CCCATGGTGG 
     3721 AATATTGCAC TCCGACTACA TCAGGAGAAG ATGTTCGAGA CTTTGCCAAG GTACTAAAAA 
     3781 ACAAATTTCG AACCAAAAGG TATTTTGCGA AGCATCCCCG AATGGGCTAC CTGCCAGTGC 
     3841 AGACTGTCTT AGAGGGGGAC AACATGGAAA CGCCTGCCTC GTCCCCTCAG CTTTCACACG 
     3901 ATGATACTCA TTCACGCATT GAACATTATG CTAGCAGGCT AGCAGAAATG GAAAACAGCA 
     3961 ATGGATCTTA TCTAAATGAT AGCATCTCTC CTAATGAGAG CATAGATGAT GAACATTTGT 
     4021 TAATCCAGCA TTACTGCCAA AGTTTGAACC AGGACTCCCC CCTGAGCCAG CCTCGTAGTC 
     4081 CTGCCCAGAT CTTGATTTCC TTAGAGAGTG AGGAAAGAGG GGAGCTAGAG AGAATCCTAG 
     4141 CAGATCTTGA GGAAGAAAAC AGGAATCTGC AAGCAGAATA TGACCGTCTA AAGCAGCAGC 
     4201 ACGAACATAA AGGCCTGTCC CCACTGCCGT CCCCTCCTGA AATGATGCCC ACCTCTCCCC 
     4261 AGAGTCCCCG GGATGCTGAG CTCATTGCTG AGGCCAAGCT ACTGCGTCAA CACAAAGGCC 
     4321 GCCTGGAAGC CAGGATGCAA ATCCTGGAAG ACCACAATAA ACAGCTGGAG TCACAGTTAC 
     4381 ACAGGCTAAG GCAGCTGCTG GAGCAACCCC AGGCAGAGGC CAAAGTGAAT GGCACAACGG 
     4441 TGTCCTCTCC TTCTACCTCT CTACAGAGGT CCGACAGCAG TCAGCCTATG CTGCTCCGAG 
     4501 TGGTTGGCAG TCAAACTTCG GACTCCATGG GTGAGGAAGA TCTTCTCAGT CCTCCCCAGG 
     4561 ACACAAGCAC AGGGTTAGAG GAGGTGATGG AGCAACTCAA CAACTCCTTC CCTAGTTCAA 
     4621 GAGGACACAA TGTAGGAAGT CTTTTCCACA TGGCAGATGA TTTGGGCAGA GCGATGGAGT 
     4681 CCTTAGTATC AGTCATGACA GATGAAGAAG GAGCAGAAAC GCGTACGCGG CCGCTCGAGC 
     4741 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4801 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4861 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4921 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4981 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5041 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5101 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5161 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5221 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5281 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5341 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5401 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5461 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5521 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5581 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5641 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5701 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5761 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5821 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5881 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5941 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6001 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6061 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6121 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6181 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6241 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6301 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6361 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6421 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6481 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6541 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6601 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6661 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6721 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6781 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6841 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6901 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6961 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7021 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7081 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7141 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7201 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7261 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7321 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7381 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7441 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7501 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7561 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7621 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7681 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7741 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7801 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7861 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7921 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7981 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8041 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8101 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8161 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8221 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8281 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8341 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8401 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8461 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8521 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//