LOCUS       RC220528                7377 bp    DNA     circular  SYN 
DEFINITION  SIDT2 (Myc-DDK-tagged)-Human SID1 transmembrane family, member 2 (SIDT2)
ACCESSION   NM_001040455
VERSION     NM_001040455.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3534..3625 
                     /label="Myc-DDK"  
     misc_feature    3655..4240 
                     /label="PolyA signal"  
     misc_feature    3712..3731 
                     /label="Restriction Enzyme"  
     misc_feature    4516..5188 
                     /label="ColE1"  
     misc_feature    5618..6412 
                     /label="Kan/Neo"  
     misc_feature    6447..6749 
                     /label="SV40 ori"  
     misc_feature    6835..7312 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3516..3522 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3516 
                     /label="SIDT2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCGCTCTG GGCTTGCCCT TCTTGGTGCT CTTGGTGGCC TCGGTCGAGA 
     1081 GCCATCTGGG GGTTCTGGGG CCCAAGAACG TCTCGCAGAA AGACGCCGAG TTTGAGCGCA 
     1141 CCTACGTGGA CGAGGTCAAC AGCGAGCTGG TCAACATCTA CACCTTCAAC CATACTGTGA 
     1201 CCCGCAACAG GACAGAGGGC GTGCGTGTGT CTGTGAACGT CCTGAACAAG CAGAAGGGGG 
     1261 CGCCGTTGCT GTTTGTGGTC CGCCAGAAGG AGGCTGTGGT GTCCTTCCAG GTGCCCCTAA 
     1321 TCCTGCGAGG GATGTTTCAG CGCAAGTACC TCTACCAAAA AGTGGAACGA ACCCTGTGTC 
     1381 AGCCCCCCAC CAAGAATGAG TCGGAGATTC AGTTCTTCTA CGTGGATGTG TCCACCCTGT 
     1441 CACCAGTCAA CACCACATAC CAGCTCCGGG TCAGCCGCAT GGACGATTTT GTGCTCAGGA 
     1501 CTGGGGAGCA GTTCAGCTTC AATACCACAG CAGCACAGCC CCAGTACTTC AAGTATGAGT 
     1561 TCCCTGAAGG CGTGGACTCG GTAATTGTCA AGGTGACCTC CAACAAGGCC TTCCCCTGCT 
     1621 CAGTCATCTC CATTCAGGAT GTGCTGTGTC CTGTCTATGA CCTGGACAAC AACGTAGCCT 
     1681 TCATCGGCAT GTACCAGACG ATGACCAAGA AGGCGGCCAT CACCGTACAG CGCAAAGACT 
     1741 TCCCCAGCAA CAGCTTTTAT GTGGTGGTGG TGGTGAAGAC CGAAGACCAA GCCTGCGGGG 
     1801 GCTCCCTGCC TTTCTACCCC TTCGCAGAAG ATGAACCGGT CGATCAAGGG CACCGCCAGA 
     1861 AAACCCTGTC AGTGCTGGTG TCTCAAGCAG TCACGTCTGA GGCATACGTC AGTGGGATGC 
     1921 TCTTTTGCCT GGGTATATTT CTCTCCTTTT ACCTGCTGAC CGTCCTCCTG GCCTGCTGGG 
     1981 AGAACTGGAG GCAGAAGAAG AAGACCCTGC TGGTGGCCAT TGACCGAGCC TGCCCAGAAA 
     2041 GCGGTCACCC TCGAGTCCTG GCTGATTCTT TTCCTGGCAG TTCCCCTTAT GAGGGTTACA 
     2101 ACTATGGCTC CTTTGAGAAT GTTTCTGGAT CTACCGATGG TCTGGTTGAC AGCGCTGGCA 
     2161 CTGGGGACCT CTCTTACGGT TACCAGGGCC GCTCCTTTGA ACCTGTAGGT ACTCGGCCCC 
     2221 GAGTGGACTC CATGAGCTCT GTGGAGGAGG ATGACTACGA CACATTGACC GACATCGATT 
     2281 CCGACAAGAA TGTCATTCGC ACCAAGCAAT ACCTCTATGT GGCTGACCTG GCACGGAAGG 
     2341 ACAAGCGTGT TCTGCGGAAA AAGTACCAGA TCTACTTCTG GAACATTGCC ACCATTGCTG 
     2401 TCTTCTATGC CCTTCCTGTG GTGCAGCTGG TGATCACCTA CCAGACGGTG GTGAATGTCA 
     2461 CAGGGAATCA GGACATCTGC TACTACAACT TCCTCTGCGC CCACCCACTG GGCAATCTCA 
     2521 GCGCCTTCAA CAACATCCTC AGCAACCTGG GGTACATCCT GCTGGGGCTG CTTTTCCTGC 
     2581 TCATCATCCT GCAACGGGAG ATCAACCACA ACCGGGCCCT GCTGCGCAAT GACCTCTGTG 
     2641 CCCTGGAATG TGGGATCCCC AAACACTTTG GGCTTTTCTA CGCCATGGGC ACAGCCCTGA 
     2701 TGATGGAGGG GCTGCTCAGT GCTTGCTATC ATGTGTGCCC CAACTATACC AATTTCCAGT 
     2761 TTGACACATC GTTCATGTAC ATGATCGCCG GACTCTGCAT GCTGAAGCTC TACCAGAAGC 
     2821 GGCACCCGGA CATCAACGCC AGCGCCTACA GTGCCTACGC CTGCCTGGCC ATTGTCATCT 
     2881 TCTTCTCTGT GCTGGGCGTG GTCTTTGGCA AAGGGAACAC GGCGTTCTGG ATCGTCTTCT 
     2941 CCATCATTCA CATCATCGCC ACCCTGCTCC TCAGCACGCA GCTCTATTAC ATGGGCCGGT 
     3001 GGAAACTGGA CTCGGGGATC TTCCGCCGCA TCCTCCACGT GCTCTACACA GACTGCATCC 
     3061 GGCAGTGCAG CGGGCCGCTC TACGTGGACC GCATGGTGCT GCTGGTCATG GGCAACGTCA 
     3121 TCAACTGGTC GCTGGCTGCC TATGGGCTTA TCATGCGCCC CAATGATTTC GCTTCCTACT 
     3181 TGTTGGCCAT TGGCATCTGC AACCTGCTCC TTTACTTCGC CTTCTACATC ATCATGAAGC 
     3241 TCCGGAGTGG GGAGAGGATC AAGCTCATCC CCCTGCTCTG CATCGTTTGC ACCTCCGTGG 
     3301 TCTGGGGCTT CGCGCTCTTC TTCTTCTTCC AGGGACTCAG CACCTGGCAG AAAACCCCTG 
     3361 CAGAGTCGAG GGAGCACAAC CGGGACTGCA TCCTCCTCGA CTTCTTTGAC GACCACGACA 
     3421 TCTGGCACTT CCTCTCCTCC ATCGCCATGT TCGGGTCCTT CCTGGTGTTG CTGACACTGG 
     3481 ATGACGACCT GGATACTGTG CAGCGGGACA AGATCTATGT CTTCACGCGT ACGCGGCCGC 
     3541 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3601 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3661 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3721 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3781 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3841 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3901 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3961 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4021 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4081 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4141 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4201 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4261 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4321 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4381 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4441 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4501 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4561 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4621 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4681 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4741 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4801 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4861 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4921 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4981 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5041 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5101 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5161 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5221 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5281 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5341 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5401 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5461 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5521 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5581 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5641 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5701 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5761 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5821 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5881 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5941 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6001 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6061 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6121 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6181 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6241 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6301 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6361 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6421 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6481 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6541 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6601 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6661 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6721 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6781 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6841 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6901 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6961 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7021 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7081 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7141 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7201 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7261 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7321 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//