LOCUS       RC220521                8226 bp    DNA     circular  SYN 
DEFINITION  DMD (Myc-DDK-tagged)-Human dystrophin (DMD), transcript variant Dp140c
ACCESSION   NM_004020
VERSION     NM_004020.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4383..4474 
                     /label="Myc-DDK"  
     misc_feature    4504..5089 
                     /label="PolyA signal"  
     misc_feature    4561..4580 
                     /label="Restriction Enzyme"  
     misc_feature    5365..6037 
                     /label="ColE1"  
     misc_feature    6467..7261 
                     /label="Kan/Neo"  
     misc_feature    7296..7598 
                     /label="SV40 ori"  
     misc_feature    7684..8161 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4365..4371 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4365 
                     /label="DMD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCATCTTCC TTGATGTTGG AGGTACCTGC TCTGGCAGAT TTCAACCGGG 
     1081 CTTGGACAGA ACTTACCGAC TGGCTTTCTC TGCTTGATCA AGTTATAAAA TCACAGAGGG 
     1141 TGATGGTGGG TGACCTTGAG GATATCAACG AGATGATCAT CAAGCAGAAG GCAACAATGC 
     1201 AGGATTTGGA ACAGAGGCGT CCCCAGTTGG AAGAACTCAT TACCGCTGCC CAAAATTTGA 
     1261 AAAACAAGAC CAGCAATCAA GAGGCTAGAA CAATCATTAC GGATCGAATT GAAAGAATTC 
     1321 AGAATCAGTG GGATGAAGTA CAAGAACACC TTCAGAACCG GAGGCAACAG TTGAATGAAA 
     1381 TGTTAAAGGA TTCAACACAA TGGCTGGAAG CTAAGGAAGA AGCTGAGCAG GTCTTAGGAC 
     1441 AGGCCAGAGC CAAGCTTGAG TCATGGAAGG AGGGTCCCTA TACAGTAGAT GCAATCCAAA 
     1501 AGAAAATCAC AGAAACCAAG CAGTTGGCCA AAGACCTCCG CCAGTGGCAG ACAAATGTAG 
     1561 ATGTGGCAAA TGACTTGGCC CTGAAACTTC TCCGGGATTA TTCTGCAGAT GATACCAGAA 
     1621 AAGTCCACAT GATAACAGAG AATATCAATG CCTCTTGGAG AAGCATTCAT AAAAGGGTGA 
     1681 GTGAGCGAGA GGCTGCTTTG GAAGAAACTC ATAGATTACT GCAACAGTTC CCCCTGGACC 
     1741 TGGAAAAGTT TCTTGCCTGG CTTACAGAAG CTGAAACAAC TGCCAATGTC CTACAGGATG 
     1801 CTACCCGTAA GGAAAGGCTC CTAGAAGACT CCAAGGGAGT AAAAGAGCTG ATGAAACAAT 
     1861 GGCAAGACCT CCAAGGTGAA ATTGAAGCTC ACACAGATGT TTATCACAAC CTGGATGAAA 
     1921 ACAGCCAAAA AATCCTGAGA TCCCTGGAAG GTTCCGATGA TGCAGTCCTG TTACAAAGAC 
     1981 GTTTGGATAA CATGAACTTC AAGTGGAGTG AACTTCGGAA AAAGTCTCTC AACATTAGGT 
     2041 CCCATTTGGA AGCCAGTTCT GACCAGTGGA AGCGTCTGCA CCTTTCTCTG CAGGAACTTC 
     2101 TGGTGTGGCT ACAGCTGAAA GATGATGAAT TAAGCCGGCA GGCACCTATT GGAGGCGACT 
     2161 TTCCAGCAGT TCAGAAGCAG AACGATGTAC ATAGGGCCTT CAAGAGGGAA TTGAAAACTA 
     2221 AAGAACCTGT AATCATGAGT ACTCTTGAGA CTGTACGAAT ATTTCTGACA GAGCAGCCTT 
     2281 TGGAAGGACT AGAGAAACTC TACCAGGAGC CCAGAGAGCT GCCTCCTGAG GAGAGAGCCC 
     2341 AGAATGTCAC TCGGCTTCTA CGAAAGCAGG CTGAGGAGGT CAATACTGAG TGGGAAAAAT 
     2401 TGAACCTGCA CTCCGCTGAC TGGCAGAGAA AAATAGATGA GACCCTTGAA AGACTCCAGG 
     2461 AACTTCAAGA GGCCACGGAT GAGCTGGACC TCAAGCTGCG CCAAGCTGAG GTGATCAAGG 
     2521 GATCCTGGCA GCCCGTGGGC GATCTCCTCA TTGACTCTCT CCAAGATCAC CTCGAGAAAG 
     2581 TCAAGGCACT TCGAGGAGAA ATTGCGCCTC TGAAAGAGAA CGTGAGCCAC GTCAATGACC 
     2641 TTGCTCGCCA GCTTACCACT TTGGGCATTC AGCTCTCACC GTATAACCTC AGCACTCTGG 
     2701 AAGACCTGAA CACCAGATGG AAGCTTCTGC AGGTGGCCGT CGAGGACCGA GTCAGGCAGC 
     2761 TGCATGAAGC CCACAGGGAC TTTGGTCCAG CATCTCAGCA CTTTCTTTCC ACGTCTGTCC 
     2821 AGGGTCCCTG GGAGAGAGCC ATCTCGCCAA ACAAAGTGCC CTACTATATC AACCACGAGA 
     2881 CTCAAACAAC TTGCTGGGAC CATCCCAAAA TGACAGAGCT CTACCAGTCT TTAGCTGACC 
     2941 TGAATAATGT CAGATTCTCA GCTTATAGGA CTGCCATGAA ACTCCGAAGA CTGCAGAAGG 
     3001 CCCTTTGCTT GGATCTCTTG AGCCTGTCAG CTGCATGTGA TGCCTTGGAC CAGCACAACC 
     3061 TCAAGCAAAA TGACCAGCCC ATGGATATCC TGCAGATTAT TAATTGTTTG ACCACTATTT 
     3121 ATGACCGCCT GGAGCAAGAG CACAACAATT TGGTCAACGT CCCTCTCTGC GTGGATATGT 
     3181 GTCTGAACTG GCTGCTGAAT GTTTATGATA CGGGACGAAC AGGGAGGATC CGTGTCCTGT 
     3241 CTTTTAAAAC TGGCATCATT TCCCTGTGTA AAGCACATTT GGAAGACAAG TACAGATACC 
     3301 TTTTCAAGCA AGTGGCAAGT TCAACAGGAT TTTGTGACCA GCGCAGGCTG GGCCTCCTTC 
     3361 TGCATGATTC TATCCAAATT CCAAGACAGT TGGGTGAAGT TGCATCCTTT GGGGGCAGTA 
     3421 ACATTGAGCC AAGTGTCCGG AGCTGCTTCC AATTTGCTAA TAATAAGCCA GAGATCGAAG 
     3481 CGGCCCTCTT CCTAGACTGG ATGAGACTGG AACCCCAGTC CATGGTGTGG CTGCCCGTCC 
     3541 TGCACAGAGT GGCTGCTGCA GAAACTGCCA AGCATCAGGC CAAATGTAAC ATCTGCAAAG 
     3601 AGTGTCCAAT CATTGGATTC AGGTACAGGA GTCTAAAGCA CTTTAATTAT GACATCTGCC 
     3661 AAAGCTGCTT TTTTTCTGGT CGAGTTGCAA AAGGCCATAA AATGCACTAT CCCATGGTGG 
     3721 AATATTGCAC TCCGACTACA TCAGGAGAAG ATGTTCGAGA CTTTGCCAAG GTACTAAAAA 
     3781 ACAAATTTCG AACCAAAAGG TATTTTGCGA AGCATCCCCG AATGGGCTAC CTGCCAGTGC 
     3841 AGACTGTCTT AGAGGGGGAC AACATGGAAA CGAATCTGCA AGCAGAATAT GACCGTCTAA 
     3901 AGCAGCAGCA CGAACATAAA GGCCTGTCCC CACTGCCGTC CCCTCCTGAA ATGATGCCCA 
     3961 CCTCTCCCCA GAGTCCCCGG GATGCTGAGC TCATTGCTGA GGCCAAGCTA CTGCGTCAAC 
     4021 ACAAAGGCCG CCTGGAAGCC AGGATGCAAA TCCTGGAAGA CCACAATAAA CAGCTGGAGT 
     4081 CACAGTTACA CAGGCTAAGG CAGCTGCTGG AGCAACCCCA GGCAGAGGCC AAAGTGAATG 
     4141 GCACAACGGT GTCCTCTCCT TCTACCTCTC TACAGAGGTC CGACAGCAGT CAGCCTATGC 
     4201 TGCTCCGAGT GGTTGGCAGT CAAACTTCGG ACTCCATGGG TGAGGAAGAT CTTCTCAGTC 
     4261 CTCCCCAGGA CACAAGCACA GGGTTAGAGG AGGTGATGGA GCAACTCAAC AACTCCTTCC 
     4321 CTAGTTCAAG AGGAAGAAAT ACCCCTGGAA AGCCAATGAG AGAGGACACA ATGACGCGTA 
     4381 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4441 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4501 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4561 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4621 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4681 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4741 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4801 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4861 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4921 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4981 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5041 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5101 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5161 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5221 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5281 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5341 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5401 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5461 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5521 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5581 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5641 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5701 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5761 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5821 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5881 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5941 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6001 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6061 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6121 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6181 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6241 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6301 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6361 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6421 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6481 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6541 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6601 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6661 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6721 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6781 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6841 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6901 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6961 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7021 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7081 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7141 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7201 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7261 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7321 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7381 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7441 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7561 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7621 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7681 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7741 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7801 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7861 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7921 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7981 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8041 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8101 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8161 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8221 AATTTT
//