LOCUS       RC220506                8793 bp    DNA     circular  SYN 
DEFINITION  NRCAM (Myc-DDK-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 1
ACCESSION   NM_001037132
VERSION     NM_001037132.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4950..5041 
                     /label="Myc-DDK"  
     misc_feature    5071..5656 
                     /label="PolyA signal"  
     misc_feature    5128..5147 
                     /label="Restriction Enzyme"  
     misc_feature    5932..6604 
                     /label="ColE1"  
     misc_feature    7034..7828 
                     /label="Kan/Neo"  
     misc_feature    7863..8165 
                     /label="SV40 ori"  
     misc_feature    8251..8728 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4932..4938 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4932 
                     /label="NRCAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCTTAAA ATAATGCCGA AAAAGAAGCG CTTATCTGCG GGCAGAGTGC 
     1081 CCCTGATTCT CTTCCTGTGC CAGATGATTA GTGCACTGGA AGTACCTCTT GATCCAAAAC 
     1141 TTCTTGAAGA CTTGGTACAG CCTCCAACCA TCACCCAACA GTCTCCAAAA GATTACATTA 
     1201 TTGACCCTCG GGAGAATATT GTAATCCAGT GTGAAGCCAA AGGGAAACCG CCCCCAAGCT 
     1261 TTTCCTGGAC CCGTAATGGG ACTCATTTTG ACATCGATAA AGACCCTCTG GTCACCATGA 
     1321 AGCCTGGCAC AGGAACGCTC ATAATTAACA TCATGAGCGA AGGGAAAGCT GAGACCTATG 
     1381 AAGGAGTCTA TCAGTGTACA GCAAGGAACG AACGCGGAGC TGCAGTTTCT AATAACATTG 
     1441 TTGTCCGCCC ATCCAGATCA CCATTGTGGA CCAAAGAAAA ACTTGAACCA ATCACACTTC 
     1501 AAAGTGGTCA GTCTTTAGTA CTTCCCTGCA GACCCCCAAT TGGATTACCA CCACCTATAA 
     1561 TATTTTGGAT GGATAATTCC TTTCAAAGAC TTCCACAAAG TGAGAGAGTT TCTCAAGGTT 
     1621 TGAATGGGGA CCTTTATTTT TCCAATGTCC TCCCAGAGGA CACCCGCGAA GACTATATCT 
     1681 GTTATGCTAG ATTTAATCAT ACTCAAACCA TACAGCAGAA GCAACCTATT TCTGTGAAGG 
     1741 TGATTTCAGT GGATGAATTG AATGACACTA TAGCTGCTAA TTTGAGTGAC ACTGAGTTTT 
     1801 ATGGTGCTAA ATCAAGTAGA GAGAGGCCAC CAACATTTTT AACTCCAGAA GGCAATGCAA 
     1861 GTAACAAAGA GGAATTAAGA GGAAATGTGC TTTCACTGGA GTGCATTGCA GAAGGACTGC 
     1921 CTACCCCAAT TATTTACTGG GCAAAGGAAG ATGGAATGCT ACCCAAAAAC AGGACAGTTT 
     1981 ATAAGAACTT TGAGAAAACC TTGCAGATCA TTCATGTTTC AGAAGCAGAC TCTGGAAATT 
     2041 ACCAATGTAT AGCAAAAAAC GCATTAGGAG CCATCCACCA TACCATTTCT GTTAGAGTTA 
     2101 AAGCGGCTCC ATACTGGATC ACAGCCCCTC AAAATCTTGT GCTGTCCCCA GGAGAGGATG 
     2161 GGACCTTGAT CTGCAGAGCT AATGGCAACC CCAAACCCAG AATTAGCTGG TTAACAAATG 
     2221 GAGTCCCAAT AGAAATTGCC CCTGATGACC CCAGCAGAAA AATAGATGGC GATACCATTA 
     2281 TTTTTTCAAA TGTTCAAGAA AGATCAAGTG CAGTCTATCA GTGCAATGCC TCTAATGAAT 
     2341 ATGGATATTT ACTGGCAAAC GCATTTGTAA ATGTGCTGGC TGAGCCACCA CGAATCCTCA 
     2401 CACCTGCAAA CACACTCTAC CAGGTCATTG CAAACAGGCC TGCTTTACTA GACTGTGCCT 
     2461 TCTTTGGGTC TCCTCTCCCA ACCATCGAGT GGTTTAAAGG AGCTAAAGGA AGTGCTCTTC 
     2521 ATGAAGATAT TTATGTTTTA CATGAAAATG GAACTTTGGA AATTCCTGTG GCCCAAAAGG 
     2581 ACAGTACAGG AACTTATACG TGTGTTGCAA GGAATAAATT AGGGATGGCG AAGAATGAAG 
     2641 TTCACTTAGA AATCAAAGAT CCTACATGGA TCGTTAAACA GCCCGAATAT GCAGTTGTGC 
     2701 AAAGAGGGAG CATGGTGTCC TTTGAATGCA AAGTGAAACA TGATCACACC TTATCCCTCA 
     2761 CTGTCCTGTG GCTGAAGGAC AACAGGGAAC TGCCCAGTGA TGAAAGGTTC ACTGTTGACA 
     2821 AGGATCATCT AGTGGTAGCT GATGTCAGTG ACGATGACAG CGGGACCTAC ACGTGTGTGG 
     2881 CCAACACCAC TCTGGACAGC GTCTCCGCCA GCGCTGTGCT TAGCGTTGTT GCTCCTACTC 
     2941 CAACTCCAGC TCCCGTTTAC GATGTCCCAA ATCCTCCCTT TGACTTAGAA CTGACAGATC 
     3001 AACTTGACAA AAGTGTTCAG CTGTCATGGA CCCCAGGCGA TGACAACAAT AGCCCCATTA 
     3061 CAAAATTCAT CATCGAATAT GAAGATGCAA TGCACAAGCC AGGGCTGTGG CACCACCAAA 
     3121 CTGAAGTTTC TGGAACACAG ACCACAGCCC AGCTGAAGCT GTCTCCTTAC GTGAACTACT 
     3181 CCTTCCGCGT GATGGCAGTG AACAGCATTG GGAAGAGCTT GCCCAGCGAG GCGTCTGAGC 
     3241 AGTATTTGAC GAAAGCCTCA GAACCAGATA AAAACCCCAC AGCTGTGGAA GGACTGGGAT 
     3301 CAGAGCCTGA TAATTTGGTG ATTACGTGGA AGCCCTTGAA TGGTTTCGAA TCTAATGGGC 
     3361 CAGGCCTTCA GTACAAAGTT AGCTGGCGCC AGAAAGATGG TGATGATGAA TGGACATCTG 
     3421 TGGTTGTGGC AAATGTATCC AAATATATTG TCTCAGGCAC GCCAACCTTT GTTCCATACC 
     3481 TGATCAAAGT TCAGGCCCTG AATGACATGG GGTTTGCCCC CGAGCCAGCT GTAGTCATGG 
     3541 GACATTCTGG AGAAGACCTC CCAATGGTGG CTCCTGGGAA CGTGCGTGTG AATGTGGTGA 
     3601 ACAGTACCTT AGCCGAGGTG CACTGGGACC CAGTACCTCT GAAAAGCATC CGAGGACACC 
     3661 TACAAGGCTA TCGGATTTAC TATTGGAAGA CCCAGAGTTC ATCTAAAAGA AACAGACGTC 
     3721 ACATTGAGAA AAAGATCCTC ACCTTCCAAG GCAGCAAGAC TCATGGCATG TTGCCGGGGC 
     3781 TAGAGCCCTT TAGCCACTAC ACACTGAATG TCCGAGTGGT CAATGGGAAA GGGGAGGGCC 
     3841 CAGCCAGCCC TGACAGAGTC TTTAATACTC CAGAAGGAGT CCCCAGTGCT CCCTCGTCTT 
     3901 TGAAGATTGT GAATCCAACA CTGGACTCTC TCACTTTGGA ATGGGATCCA CCGAGCCACC 
     3961 CGAATGGCAT TTTGACAGAG TACACCTTAA AGTATCAGCC AATTAACAGC ACACATGAAT 
     4021 TAGGCCCTCT GGTAGATTTG AAAATTCCTG CCAACAAGAC ACGGTGGACT TTAAAAAATT 
     4081 TAAATTTCAG CACTCGATAT AAGTTTTATT TCTATGCACA AACATCAGCA GGATCAGGAA 
     4141 GTCAAATTAC AGAGGAAGCA GTAACAACTG TGGATGAAGC TGGTATTCTT CCACCTGATG 
     4201 TAGGTGCAGG CAAAGTTCAA GCAGTAAATC CCAGGATCAG CAATCTTACT GCTGCAGCTG 
     4261 CTGAGACCTA TGCCAATATC AGTTGGGAAT ATGAGGGACC AGAGCATGTG AACTTTTATG 
     4321 TTGAATATGG TGTAGCAGGC AGCAAAGAAG AATGGAGAAA AGAAATTGTA AATGGTTCTC 
     4381 GGAGCTTCTT TGGGTTAAAG GGTCTAATGC CAGGAACAGC ATACAAAGTT CGAGTTGGTG 
     4441 CTGTGGGGGA CTCTGGTTTT GTGAGTTCAG AGGATGTGTT TGAGACAGGC CCAGCGATGG 
     4501 CAAGCCGGCA GGTGGATATT GCAACTCAGG GCTGGTTCAT TGGTCTGATG TGTGCTGTTG 
     4561 CTCTCCTTAT CTTAATTTTG CTGATTGTTT GCTTCATCAG AAGAAACAAG GGTGGTAAAT 
     4621 ATCCAGTTAA AGAAAAGGAA GATGCCCATG CTGACCCTGA AATCCAGCCT ATGAAGGAAG 
     4681 ATGATGGGAC ATTTGGAGAA TACAGTGATG CAGAAGACCA CAAGCCTTTG AAAAAAGGAA 
     4741 GTCGAACTCC TTCAGACAGG ACTGTGAAAA AAGAAGATAG TGACGACAGC CTAGTTGACT 
     4801 ATGGAGAAGG GGTTAATGGC CAGTTCAATG AGGATGGCTC CTTTATTGGA CAATACAGTG 
     4861 GTAAGAAAGA GAAAGAGCCG GCTGAAGGAA ACGAAAGCTC AGAGGCACCT TCTCCTGTCA 
     4921 ACGCCATGAA TTCCTTTGTT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4981 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5041 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5101 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5161 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5221 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5281 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5341 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5401 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5461 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5521 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5581 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5641 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5701 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5761 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5821 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5881 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5941 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6001 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6061 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6121 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6181 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6241 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6301 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6361 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6421 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6481 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6541 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6601 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6661 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6721 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6781 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6841 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6901 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6961 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7021 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7081 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7141 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7201 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7261 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7321 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7381 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7441 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7501 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7561 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7621 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7681 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7741 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7801 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7861 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7921 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7981 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8041 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8101 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8161 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8221 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8281 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8341 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8401 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8461 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8521 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8581 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8641 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8701 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8761 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//