LOCUS       RC220468                8490 bp    DNA     circular  SYN 
DEFINITION  CGN (Myc-DDK-tagged)-Human cingulin (CGN)
ACCESSION   NM_020770
VERSION     NM_020770.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4647..4738 
                     /label="Myc-DDK"  
     misc_feature    4768..5353 
                     /label="PolyA signal"  
     misc_feature    4825..4844 
                     /label="Restriction Enzyme"  
     misc_feature    5629..6301 
                     /label="ColE1"  
     misc_feature    6731..7525 
                     /label="Kan/Neo"  
     misc_feature    7560..7862 
                     /label="SV40 ori"  
     misc_feature    7948..8425 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4629..4635 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4629 
                     /label="CGN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCAGGCA CCCAACATGG CTGAGCCCCG GGGCCCCGTA GACCATGGAG 
     1081 TCCAGATTCG CTTCATCACA GAGCCAGTGA GTGGTGCAGA GATGGGCACT CTACGTCGAG 
     1141 GTGGACGACG CCCAGCTAAG GATGCAAGAG CCAGTACCTA CGGGGTTGCT GTGCGTGTGC 
     1201 AGGGAATCGC TGGGCAGCCC TTTGTGGTGC TCAACAGTGG GGAGAAAGGC GGTGACTCCT 
     1261 TTGGGGTCCA AATCAAGGGG GCCAATGACC AAGGGGCCTC AGGAGCTCTG AGCTCAGATT 
     1321 TGGAACTCCC TGAGAACCCC TACTCTCAGG TCAAGGGATT TCCTGCCCCC TCGCAGAGCA 
     1381 GCACATCTGA TGAGGAGCCT GGGGCCTACT GGAATGGAAA GCTACTCCGT TCCCACTCCC 
     1441 AGGCCTCACT GGCAGGCCCT GGCCCAGTGG ATCCTAGTAA CAGAAGCAAC AGCATGCTGG 
     1501 AGCTAGCCCC GAAAGTGGCT TCCCCAGGTA GCACCATTGA CACTGCTCCC CTGTCTTCAG 
     1561 TGGACTCACT CATCAACAAG TTTGACAGTC AACTTGGAGG CCAGGCCCGG GGTCGGACTG 
     1621 GCCGCCGAAC ACGGATGCTA CCCCCTGAAC AGCGCAAACG GAGCAAGAGC CTGGACAGCC 
     1681 GCCTCCCACG GGACACCTTT GAGGAACGGG AGCGCCAGTC CACCAACCAC TGGACCTCTA 
     1741 GCACAAAATA TGACAACCAT GTGGGCACTT CGAAGCAGCC AGCCCAGAGC CAGAACCTGA 
     1801 GTCCTCTCAG TGGCTTTAGC CGTTCTCGTC AGACTCAGGA CTGGGTCCTT CAGAGTTTTG 
     1861 AGGAGCCGCG GAGGAGTGCA CAGGACCCCA CCATGCTGCA GTTCAAATCA ACTCCAGACC 
     1921 TCCTTCGAGA CCAGCAGGAG GCAGCCCCAC CAGGCAGTGT GGACCATATG AAGGCCACCA 
     1981 TCTATGGCAT CCTGAGGGAG GGAAGCTCAG AAAGTGAAAC CTCTGTGAGG AGGAAGGTTA 
     2041 GTTTGGTGCT GGAGAAGATG CAGCCTCTAG TGATGGTTTC TTCTGGTTCT ACTAAGGCCG 
     2101 TGGCAGGGCA GGGTGAGCTT ACCCGAAAAG TGGAGGAGCT ACAGCGAAAG CTGGATGAAG 
     2161 AGGTGAAGAA GCGGCAGAAG CTAGAGCCAT CCCAAGTTGG GCTGGAGCGG CAGCTGGAGG 
     2221 AGAAAACAGA AGAGTGCAGC CGACTGCAGG AGCTGCTGGA GAGGAGGAAG GGGGAGGCCC 
     2281 AGCAGAGCAA CAAGGAGCTC CAGAACATGA AGCGCCTCTT GGACCAGGGT GAAGATTTAC 
     2341 GACATGGGCT GGAGACCCAG GTGATGGAGC TGCAGAACAA GCTGAAACAT GTCCAGGGTC 
     2401 CTGAGCCTGC TAAGGAGGTG TTACTGAAGG ACCTGTTAGA GACCCGGGAA CTTCTGGAAG 
     2461 AGGTCTTGGA GGGGAAACAG CGAGTAGAGG AGCAGCTGAG GCTGCGGGAG CGGGAGTTGA 
     2521 CAGCCCTGAA GGGGGCCCTG AAAGAGGAGG TAGCCTCCCG TGACCAGGAG GTGGAACATG 
     2581 TCCGGCAGCA GTACCAGCGA GACACAGAGC AGCTCCGCAG GAGCATGCAA GATGCAACCC 
     2641 AGGACCATGC AGTGCTGGAG GCCGAGAGGC AGAAGATGTC AGCCCTTGTG CGAGGGCTGC 
     2701 AGAGGGAGCT GGAGGAGACT TCAGAGGAGA CAGGGCATTG GCAGAGTATG TTCCAGAAGA 
     2761 ACAAGGAGGA TCTTAGAGCC ACCAAGCAGG AACTCCTGCA GCTGCGAATG GAGAAGGAGG 
     2821 AGATGGAAGA GGAGCTTGGA GAGAAGATAG AGGTCTTGCA GAGGGAATTA GAGCAGGCCC 
     2881 GAGCTAGTGC TGGAGATACT CGCCAGGTTG AGGTGCTCAA GAAGGAGCTG CTCCGGACAC 
     2941 AGGAGGAGCT TAAGGAACTG CAGGCAGAAC GGCAGAGCCA GGAGGTGGCT GGGCGACACC 
     3001 GGGACCGGGA GTTGGAGAAG CAGCTGGCGG TCCTGAGGGT CGAGGCTGAT CGAGGTCGGG 
     3061 AGCTGGAAGA ACAGAACCTC CAGCTACAAA AGACCCTCCA GCAACTGCGA CAGGACTGTG 
     3121 AAGAGGCTTC CAAGGCTAAG ATGGTGGCCG AGGCAGAGGC AACAGTGCTG GGGCAGCGGC 
     3181 GGGCCGCAGT GGAGACGACG CTTCGGGAGA CCCAGGAGGA AAATGACGAA TTCCGCCGGC 
     3241 GCATCCTGGG TTTGGAGCAG CAGCTGAAGG AGACTCGAGG TCTGGTGGAT GGTGGGGAAG 
     3301 CGGTGGAGGC ACGACTACGG GACAAGCTGC AGCGGCTGGA GGCAGAGAAA CAGCAGCTGG 
     3361 AGGAGGCCCT GAATGCGTCC CAGGAAGAGG AGGGGAGTCT GGCAGCAGCC AAGCGGGCAC 
     3421 TGGAGGCACG CCTAGAGGAG GCTCAGCGGG GGCTGGCCCG CCTGGGGCAG GAGCAGCAGA 
     3481 CACTGAACCG GGCCCTGGAG GAGGAAGGGA AGCAGCGGGA GGTGCTCCGG CGAGGCAAGG 
     3541 CTGAGCTGGA GGAGCAGAAG CGTTTGCTGG ACAGGACTGT GGACCGACTG AACAAGGAGT 
     3601 TGGAGAAGAT CGGGGAGGAC TCTAAGCAAG CCCTGCAGCA GCTCCAGGCC CAGCTGGAGG 
     3661 ATTATAAGGA AAAGGCCCGG CGGGAGGTGG CAGATGCCCA GCGCCAGGCC AAGGATTGGG 
     3721 CCAGTGAGGC TGAGAAGACC TCTGGAGGAC TGAGCCGACT TCAGGATGAG ATCCAGAGGC 
     3781 TGCGGCAGGC CCTGCAGGCA TCCCAGGCTG AGCGGGACAC AGCCCGGCTG GACAAAGAGC 
     3841 TACTGGCCCA GCGACTGCAG GGGCTGGAGC AAGAGGCAGA GAACAAGAAG CGTTCCCAGG 
     3901 ACGACAGGGC CCGGCAGCTG AAGGGTCTCG AGGAAAAAGT CTCACGGCTG GAAACAGAGT 
     3961 TAGATGAGGA GAAGAACACC GTGGAGCTGC TAACAGATCG GGTGAATCGT GGCCGGGACC 
     4021 AGGTGGATCA GCTGAGGACA GAGCTCATGC AGGAAAGGTC TGCTCGGCAG GACCTGGAGT 
     4081 GTGACAAAAT CTCCTTGGAG AGACAGAACA AGGACCTGAA GACCCGGTTG GCCAGCTCAG 
     4141 AAGGCTTCCA GAAGCCTAGT GCCAGCCTCT CTCAGCTTGA GTCCCAGAAT CAGTTGTTGC 
     4201 AGGAGCGGCT ACAGGCTGAA GAGAGGGAGA AGACAGTTCT GCAGTCTACC AATCGAAAAC 
     4261 TGGAGCGGAA AGTTAAAGAA CTATCCATCC AGATTGAAGA CGAGCGGCAG CATGTCAATG 
     4321 ACCAGAAAGA CCAGCTAAGC CTGAGGGTGA AGGCTTTGAA GCGTCAGGTG GATGAAGCAG 
     4381 AAGAGGAAAT TGAGCGACTG GACGGCCTGA GGAAGAAGGC CCAGCGTGAG GTGGAGGAGC 
     4441 AGCATGAGGT CAATGAACAG CTCCAGGCCC GGATCAAGTC TCTGGAGAAG GACTCCTGGC 
     4501 GCAAAGCTTC CCGCTCAGCT GCTGAGTCAG CTCTCAAAAA CGAAGGGCTG AGCTCAGATG 
     4561 AGGAATTCGA CAGTGTCTAC GATCCCTCGT CCATTGCATC ACTGCTTACG GAGAGCAACC 
     4621 TACAGACCAG CTCCTGTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4681 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4741 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4801 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4861 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4921 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4981 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5041 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5101 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5161 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5221 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5281 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5341 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5401 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5461 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5521 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5581 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5641 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5701 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5761 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5821 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5881 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5941 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6001 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6061 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6121 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6181 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6241 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6301 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6361 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6421 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6481 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6541 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6601 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6661 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6721 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6781 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6841 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6901 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6961 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7021 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7081 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7141 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7201 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7261 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7321 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7381 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7441 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7501 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7561 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7621 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7681 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7741 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7861 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7921 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7981 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8041 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8101 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8161 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8221 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8281 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8341 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8401 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8461 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//