LOCUS RC220459 8022 bp DNA circular SYN DEFINITION NPR2 (Myc-DDK-tagged)-Human natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide receptor B) (NPR2) ACCESSION NM_003995 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4179..4270 /label="Myc-DDK" misc_feature 4300..4885 /label="PolyA signal" misc_feature 4357..4376 /label="Restriction Enzyme" misc_feature 5161..5833 /label="ColE1" misc_feature 6263..7057 /label="Kan/Neo" misc_feature 7092..7394 /label="SV40 ori" misc_feature 7480..7957 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4161..4167 /label="Restriction Enzyme" misc_feature 1020..4161 /label="NPR2" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCGCTGCCA TCACTTCTGC TGTTGGTGGC AGCCCTGGCA GGTGGGGTGC 1081 GTCCTCCCGG GGCGCGGAAC CTGACGCTGG CGGTGGTGCT GCCAGAACAC AACCTGAGCT 1141 ATGCCTGGGC CTGGCCACGG GTGGGACCCG CTGTGGCACT AGCTGTGGAG GCTCTGGGCC 1201 GGGCACTGCC CGTGGACCTG CGGTTTGTCA GCTCCGAACT GGAAGGCGCC TGCTCTGAGT 1261 ACCTGGCACC GCTGAGCGCT GTGGACCTCA AGCTGTACCA TGACCCCGAC CTGCTGTTAG 1321 GTCCCGGTTG CGTGTACCCT GCTGCCTCTG TGGCCCGCTT TGCCTCCCAC TGGCGCCTTC 1381 CCCTGCTGAC TGCGGGTGCT GTGGCCTCTG GTTTTTCGGC TAAGAATGAC CATTATCGTA 1441 CCCTGGTTCG CACTGGCCCC TCTGCTCCCA AGCTGGGTGA GTTTGTGGTG ACACTACACG 1501 GGCACTTCAA TTGGACTGCC CGTGCTGCCT TGCTGTACCT GGATGCTCGC ACAGATGACC 1561 GGCCTCACTA CTTCACCATC GAGGGCGTCT TTGAGGCCCT GCAGGGCAGC AACCTCAGTG 1621 TGCAGCACCA GGTGTATGCC CGAGAGCCAG GGGGCCCCGA GCAGGCCACC CACTTCATCC 1681 GGGCCAACGG GCGCATTGTG TATATCTGCG GCCCTCTGGA GATGCTGCAT GAGATCCTGC 1741 TTCAGGCCCA GAGGGAGAAT CTGACCAATG GGGATTATGT CTTCTTTTAC CTGGATGTCT 1801 TTGGGGAGAG TCTCCGTGCA GGCCCCACAC GTGCTACAGG CCGGCCCTGG CAGGACAATC 1861 GCACCCGGGA ACAGGCCCAG GCCCTCAGAG AGGCCTTTCA GACTGTATTG GTGATCACGT 1921 ACCGAGAACC CCCAAATCCT GAGTATCAGG AATTCCAGAA TCGTCTGCTG ATAAGAGCCC 1981 GGGAAGACTT TGGTGTGGAG CTGGGCCCTT CCCTGATGAA CCTCATCGCT GGCTGCTTCT 2041 ATGATGGGAT CCTGCTATAT GCTGAAGTCC TGAATGAGAC AATACAGGAA GGAGGCACCC 2101 GGGAGGATGG ACTTCGAATT GTGGAAAAGA TGCAGGGACG AAGATATCAC GGTGTAACTG 2161 GGCTGGTTGT CATGGACAAG AACAATGACC GAGAGACTGA CTTTGTCCTC TGGGCCATGG 2221 GAGACCTGGA TTCTGGGGAC TTTCAGCCTG CAGCCCACTA CTCGGGAGCT GAGAAGCAGA 2281 TTTGGTGGAC GGGACGGCCT ATTCCCTGGG TGAAGGGGGC TCCTCCCTCG GACAATCCCC 2341 CCTGTGCCTT TGACTTGGAC GACCCATCCT GTGATAAAAC TCCACTTTCA ACCCTGGCAA 2401 TTGTGGCTCT GGGCACAGGA ATCACCTTCA TCATGTTTGG TGTTTCCAGC TTCCTAATTT 2461 TCCGAAAGCT GATGCTGGAG AAGGAGCTGG CTAGCATGTT GTGGCGTATT CGCTGGGAAG 2521 AACTGCAGTT TGGCAACTCA GAGCGTTATC ACAAAGGTGC AGGCAGTCGC CTCACACTGT 2581 CGCTGCGGGG ATCCAGTTAC GGCTCGCTCA TGACAGCCCA TGGGAAATAC CAGATCTTTG 2641 CCAACACCGG TCACTTCAAG GGAAATGTTG TCGCCATCAA ACATGTGAAT AAGAAGCGCA 2701 TTGAGCTGAC CCGGCAGGTT CTGTTTGAAC TCAAACATAT GAGAGATGTT CAGTTCAACC 2761 ATCTCACTCG CTTCATTGGC GCCTGCATAG ACCCTCCCAA CATTTGCATT GTCACTGAAT 2821 ATTGTCCTCG TGGGAGTTTA CAGGATATTC TAGAAAATGA CAGCATCAAC TTGGACTGGA 2881 TGTTTCGTTA TTCACTCATT AATGACCTTG TTAAGGGCAT GGCCTTTCTC CACAACAGCA 2941 TTATTTCATC GCATGGGAGT CTCAAGTCCT CCAACTGTGT GGTGGATAGT CGTTTTGTGC 3001 TCAAAATCAC AGACTATGGC CTGGCCAGCT TCCGATCAAC TGCTGAACCT GATGACAGCC 3061 ATGCCCTCTA TGCCAAGAAG CTGTGGACTG CCCCAGAACT GCTCAGTGGG AACCCCTTGC 3121 CAACCACAGG CATGCAGAAG GCTGACGTCT ATAGCTTTGG GATCATCCTG CAGGAGATAG 3181 CACTTCGCAG TGGTCCTTTC TACTTGGAGG GCCTGGACCT CAGCCCCAAA GAGATTGTCC 3241 AGAAGGTACG AAATGGTCAG CGGCCATATT TCCGGCCAAG CATTGACCGG ACCCAACTGA 3301 ATGAAGAGCT AGTTTTGCTG ATGGAGCGAT GTTGGGCTCA GGACCCAGCT GAGCGGCCAG 3361 ACTTTGGACA GATTAAGGGC TTCATTCGGC GCTTTAACAA GGAGGGTGGC ACCAGCATAT 3421 TGGACAACCT CCTGCTGCGC ATGGAACAGT ATGCCAATAA CTTGGAGAAG CTGGTGGAGG 3481 AACGCACACA GGCCTATCTG GAGGAAAAAC GCAAGGCTGA AGCTCTGCTC TACCAAATCC 3541 TACCCCATTC AGTGGCAGAG CAGTTAAAAC GGGGAGAGAC TGTACAGGCT GAGGCCTTTG 3601 ACAGTGTTAC CATCTACTTC AGTGACATTG TTGGCTTCAC AGCATTGTCA GCAGAGAGCA 3661 CCCCCATGCA GGTAGTGACA CTTCTTAATG ACCTGTATAC CTGCTTTGAT GCCATAATTG 3721 ACAACTTTGA TGTCTACAAG GTGGAGACGA TTGGGGATGC TTACATGGTG GTATCTGGCC 3781 TCCCAGGCCG AAATGGTCAA CGCCATGCAC CAGAAATTGC TCGTATGGCC CTAGCATTAC 3841 TAGATGCAGT TTCTTCCTTT CGCATCCGCC ACCGACCCCA TGACCAGCTG AGGCTACGCA 3901 TAGGGGTCCA TACTGGGCCA GTCTGTGCTG GGGTTGTTGG CCTGAAGATG CCCCGTTATT 3961 GTCTTTTTGG AGACACAGTG AACACTGCTT CTCGAATGGA GTCTAATGGT CAAGCGCTGA 4021 AGATCCATGT CTCCTCTACC ACCAAGGATG CCCTAGATGA GCTAGGATGC TTCCAGCTAG 4081 AGCTTCGGGG GGATGTGGAA ATGAAGGGAA AAGGAAAGAT GCGAACATAC TGGCTCTTAG 4141 GAGAGCGGAA AGGACCTCCT GGACTCCTGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 4201 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 4261 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 4321 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 4381 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 4441 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 4501 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 4561 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 4621 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 4681 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 4741 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 4801 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 4861 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 4921 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 4981 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 5041 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 5101 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 5161 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 5221 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 5281 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 5341 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 5401 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 5461 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 5521 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 5581 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 5641 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 5701 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 5761 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 5821 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 5881 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 5941 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 6001 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 6061 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 6121 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 6181 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 6241 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 6301 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 6361 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 6421 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 6481 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 6541 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 6601 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 6661 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 6721 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 6781 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 6841 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 6901 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 6961 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 7021 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 7081 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 7141 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 7201 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 7261 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 7321 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 7381 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 7441 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 7501 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 7561 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 7621 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 7681 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 7741 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 7801 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 7861 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 7921 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 7981 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT //