LOCUS       RC220459                8022 bp    DNA     circular  SYN 
DEFINITION  NPR2 (Myc-DDK-tagged)-Human natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide receptor B) (NPR2)
ACCESSION   NM_003995
VERSION     NM_003995.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4179..4270 
                     /label="Myc-DDK"  
     misc_feature    4300..4885 
                     /label="PolyA signal"  
     misc_feature    4357..4376 
                     /label="Restriction Enzyme"  
     misc_feature    5161..5833 
                     /label="ColE1"  
     misc_feature    6263..7057 
                     /label="Kan/Neo"  
     misc_feature    7092..7394 
                     /label="SV40 ori"  
     misc_feature    7480..7957 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4161..4167 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4161 
                     /label="NPR2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGCCA TCACTCCTGC TGTTGGTGGC AGCCCTGGCA GGTGGGGTGC 
     1081 GTCCTCCCGG GGCGCGGAAC CTGACGCTGG CGGTGGTGCT GCCAGAACAC AACCTGAGCT 
     1141 ATGCCTGGGC CTGGCCACGG GTGGGACCCG CTGTGGCACT AGCTGTGGAG GCTCTGGGCC 
     1201 GGGCACTGCC CGTGGACCTG CGGTTTGTCA GCTCCGAACT GGAAGGCGCC TGCTCTGAGT 
     1261 ACCTGGCACC GCTGAGCGCT GTGGACCTCA AGCTGTACCA TGACCCCGAC CTGCTGTTAG 
     1321 GTCCCGGTTG CGTGTACCCT GCTGCCTCTG TGGCCCGCTT TGCCTCCCAC TGGCGCCTTC 
     1381 CCCTGCTGAC TGCGGGTGCT GTGGCCTCTG GTTTTTCGGC TAAGAATGAC CATTATCGTA 
     1441 CCCTGGTTCG CACTGGCCCC TCTGCTCCCA AGCTGGGTGA GTTTGTGGTG ACACTACACG 
     1501 GGCACTTCAA TTGGACTGCC CGTGCTGCCT TGCTGTACCT GGATGCTCGC ACAGATGACC 
     1561 GGCCTCACTA CTTCACCATC GAGGGCGTCT TTGAGGCCCT GCAGGGCAGC AACCTCAGTG 
     1621 TGCAGCACCA GGTGTATGCC CGAGAGCCAG GGGGCCCCGA GCAGGCCACC CACTTCATCC 
     1681 GGGCCAACGG GCGCATTGTG TATATCTGCG GCCCTCTGGA GATGCTGCAT GAGATCCTGC 
     1741 TTCAGGCCCA GAGGGAGAAT CTGACCAATG GGGATTATGT CTTCTTTTAC CTGGATGTCT 
     1801 TTGGGGAGAG TCTCCGTGCA GGCCCCACAC GTGCTACAGG CCGGCCCTGG CAGGACAATC 
     1861 GCACCCGGGA ACAGGCCCAG GCCCTCAGAG AGGCCTTTCA GACTGTATTG GTGATCACGT 
     1921 ACCGAGAACC CCCAAATCCT GAGTATCAGG AATTCCAGAA TCGTCTGCTG ATAAGAGCCC 
     1981 GGGAAGACTT TGGTGTGGAG CTGGGCCCTT CCCTGATGAA CCTCATCGCT GGCTGCTTCT 
     2041 ATGATGGGAT CCTGCTATAT GCTGAAGTCC TGAATGAGAC AATACAGGAA GGAGGCACCC 
     2101 GGGAGGATGG ACTTCGAATT GTGGAAAAGA TGCAGGGACG AAGATATCAC GGTGTAACTG 
     2161 GGCTGGTTGT CATGGACAAG AACAATGACC GAGAGACTGA CTTTGTCCTC TGGGCCATGG 
     2221 GAGACCTGGA TTCTGGGGAC TTTCAGCCTG CAGCCCACTA CTCGGGAGCT GAGAAGCAGA 
     2281 TTTGGTGGAC GGGACGGCCT ATTCCCTGGG TGAAGGGGGC TCCTCCCTCG GACAATCCCC 
     2341 CCTGTGCCTT TGACTTGGAC GACCCATCCT GTGATAAAAC TCCACTTTCA ACCCTGGCAA 
     2401 TTGTGGCTCT GGGCACAGGA ATCACCTTCA TCATGTTTGG TGTTTCCAGC TTCCTAATTT 
     2461 TCCGAAAGCT GATGCTGGAG AAGGAGCTGG CTAGCATGTT GTGGCGTATT CGCTGGGAAG 
     2521 AACTGCAGTT TGGCAACTCA GAGCGTTATC ACAAAGGTGC AGGCAGTCGC CTCACACTGT 
     2581 CGCTGCGGGG ATCCAGTTAC GGCTCGCTCA TGACAGCCCA TGGGAAATAC CAGATCTTTG 
     2641 CCAACACCGG TCACTTCAAG GGAAATGTTG TCGCCATCAA ACATGTGAAT AAGAAGCGCA 
     2701 TTGAGCTGAC CCGGCAGGTT CTGTTTGAAC TCAAACATAT GAGAGATGTT CAGTTCAACC 
     2761 ATCTCACTCG CTTCATTGGC GCCTGCATAG ACCCTCCCAA CATTTGCATT GTCACTGAAT 
     2821 ATTGTCCTCG TGGGAGTTTA CAGGATATTC TAGAAAATGA CAGCATCAAC TTGGACTGGA 
     2881 TGTTTCGTTA TTCACTCATT AATGACCTTG TTAAGGGCAT GGCCTTTCTC CACAACAGCA 
     2941 TTATTTCATC GCATGGGAGT CTCAAGTCCT CCAACTGTGT GGTGGATAGT CGTTTTGTGC 
     3001 TCAAAATCAC AGACTATGGC CTGGCCAGCT TCCGATCAAC TGCTGAACCT GATGACAGCC 
     3061 ATGCCCTCTA TGCCAAGAAG CTGTGGACTG CCCCAGAACT GCTCAGTGGG AACCCCTTGC 
     3121 CAACCACAGG CATGCAGAAG GCTGACGTCT ATAGCTTTGG GATCATCCTG CAGGAGATAG 
     3181 CACTTCGCAG TGGTCCTTTC TACTTGGAGG GCCTGGACCT CAGCCCCAAA GAGATTGTCC 
     3241 AGAAGGTACG AAATGGTCAG CGGCCATATT TCCGGCCAAG CATTGACCGG ACCCAACTGA 
     3301 ATGAAGAGCT AGTTTTGCTG ATGGAGCGAT GTTGGGCTCA GGACCCAGCT GAGCGGCCAG 
     3361 ACTTTGGACA GATTAAGGGC TTCATTCGGC GCTTTAACAA GGAGGGTGGC ACCAGCATAT 
     3421 TGGACAACCT CCTGCTGCGC ATGGAACAGT ATGCCAATAA CTTGGAGAAG CTGGTGGAGG 
     3481 AACGCACACA GGCCTATCTG GAGGAAAAAC GCAAGGCTGA AGCTCTGCTC TACCAAATCC 
     3541 TACCCCATTC AGTGGCAGAG CAGTTAAAAC GGGGAGAGAC TGTACAGGCT GAGGCCTTTG 
     3601 ACAGTGTTAC CATCTACTTC AGTGACATTG TTGGCTTCAC AGCATTGTCA GCAGAGAGCA 
     3661 CCCCCATGCA GGTAGTGACA CTTCTTAATG ACCTGTATAC CTGCTTTGAT GCCATAATTG 
     3721 ACAACTTTGA TGTCTACAAG GTGGAGACGA TTGGGGATGC TTACATGGTG GTATCTGGCC 
     3781 TCCCAGGCCG AAATGGTCAA CGCCATGCAC CAGAAATTGC TCGTATGGCC CTAGCATTAC 
     3841 TAGATGCAGT TTCTTCCTTT CGCATCCGCC ACCGACCCCA TGACCAGCTG AGGCTACGCA 
     3901 TAGGGGTCCA TACTGGGCCA GTCTGTGCTG GGGTTGTTGG CCTGAAGATG CCCCGTTATT 
     3961 GTCTTTTTGG AGACACAGTG AACACTGCTT CTCGAATGGA GTCTAATGGT CAAGCGCTGA 
     4021 AGATCCATGT CTCCTCTACC ACCAAGGATG CCCTAGATGA GCTAGGATGC TTCCAGCTAG 
     4081 AGCTTCGGGG GGATGTGGAA ATGAAGGGAA AAGGAAAGAT GCGAACATAC TGGCTCTTAG 
     4141 GAGAGCGGAA AGGACCTCCT GGACTCCTGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4201 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4261 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4321 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4381 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4441 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4501 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4561 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4621 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4681 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4741 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4801 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4861 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4921 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4981 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5041 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5101 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5161 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5221 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5281 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5341 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5401 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5461 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5521 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5581 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5641 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5701 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5761 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5821 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5881 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5941 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6001 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6061 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6121 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6181 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6241 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6301 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6361 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6421 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6481 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6541 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6601 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6661 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6721 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6781 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6841 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6901 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6961 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7021 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7081 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7141 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7201 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7261 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7321 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7381 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7441 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7501 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7561 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7621 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7681 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7741 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7801 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7861 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7921 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7981 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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