LOCUS       RC220408L2                9621 bp    DNA     circular  SYN 
DEFINITION  ITCH (Myc-DDK-tagged)-Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH)
ACCESSION   NM_031483
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7912..8092 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8820..9479 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    8140..8759 
                     /label="ColE1"  
     misc_feature    6787..6807 
                     /label="LR50"  
     misc_feature    5927..6652 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6796..7387 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5906..5912 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5906 
                     /label="ITCH"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCTGACAGT GGATCACAAC TTGGTTCAAT 
     3361 GGGTAGCCTC ACCATGAAAT CACAGCTTCA GATCACTGTC ATCTCAGCAA AACTTAAGGA 
     3421 AAATAAGAAG AATTGGTTTG GACCAAGTCC TTACGTAGAG GTCACAGTAG ATGGACAGTC 
     3481 AAAGAAGACA GAAAAATGCA ACAACACAAA CAGTCCCAAG TGGAAGCAAC CCCTTACAGT 
     3541 TATCGTTACC CCTGTGAGTA AATTACATTT TCGTGTGTGG AGTCACCAGA CACTGAAATC 
     3601 TGATGTTTTG TTGGGAACTG CTGCATTAGA TATTTATGAA ACATTAAAGT CAAACAATAT 
     3661 GAAACTTGAA GAAGTAGTTG TGACTTTGCA GCTTGGAGGT GACAAAGAGC CAACAGAGAC 
     3721 AATAGGAGAC TTGTCAATTT GTCTTGATGG GCTACAGTTA GAGTCTGAAG TTGTTACCAA 
     3781 TGGTGAAACT ACATGTTCAG AAAGTGCTTC TCAGAATGAT GATGGCTCCA GATCCAAGGA 
     3841 TGAAACAAGA GTGAGCACAA ATGGATCAGA TGACCCTGAA GATGCAGGAG CTGGTGAAAA 
     3901 TAGGAGAGTC AGTGGGAATA ATTCTCCATC ACTCTCAAAT GGTGGTTTTA AACCTTCTAG 
     3961 ACCTCCAAGA CCTTCACGAC CACCACCACC CACCCCACGT AGACCAGCAT CTGTCAATGG 
     4021 TTCACCATCT GCCACTTCTG AAAGTGATGG GTCTAGTACA GGCTCTCTGC CGCCGACAAA 
     4081 TACAAATACA AATACATCTG AAGGAGCAAC ATCTGGATTA ATAATTCCTC TTACTATATC 
     4141 TGGAGGCTCA GGCCCTAGGC CATTAAATCC TGTAACTCAA GCTCCCTTGC CACCTGGTTG 
     4201 GGAGCAGAGA GTGGACCAGC ACGGGCGAGT TTACTATGTA GATCATGTTG AGAAAAGAAC 
     4261 AACATGGGAT AGACCAGAAC CTCTACCTCC TGGCTGGGAA CGGCGGGTTG ACAACATGGG 
     4321 ACGTATTTAT TATGTTGACC ATTTCACAAG AACAACAACG TGGCAGAGGC CAACACTGGA 
     4381 ATCCGTCCGG AACTATGAAC AATGGCAGCT ACAGCGTAGT CAGCTTCAAG GAGCAATGCA 
     4441 GCAGTTTAAC CAGAGATTCA TTTATGGGAA TCAAGATTTA TTTGCTACAT CACAAAGTAA 
     4501 AGAATTTGAT CCTCTTGGTC CATTGCCACC TGGATGGGAG AAGAGAACAG ACAGCAATGG 
     4561 CAGAGTATAT TTCGTCAACC ACAACACACG AATTACACAA TGGGAAGACC CCAGAAGTCA 
     4621 AGGTCAATTA AATGAAAAGC CCTTACCTGA AGGTTGGGAA ATGAGATTCA CAGTGGATGG 
     4681 AATTCCATAT TTTGTGGACC ACAATAGAAG AACTACCACC TATATAGATC CCCGCACAGG 
     4741 AAAATCTGCC CTAGACAATG GACCTCAGAT AGCCTATGTT CGGGACTTCA AAGCAAAGGT 
     4801 TCAGTATTTC CGGTTCTGGT GTCAGCAACT GGCCATGCCA CAGCACATAA AGATTACAGT 
     4861 GACAAGAAAA ACATTGTTTG AGGATTCCTT TCAACAGATA ATGAGCTTCA GTCCCCAAGA 
     4921 TCTGCGAAGA CGTTTGTGGG TGATTTTTCC AGGAGAAGAA GGTTTAGATT ATGGAGGTGT 
     4981 AGCAAGAGAA TGGTTCTTTC TTTTGTCACA TGAAGTGTTG AACCCAATGT ATTGCCTGTT 
     5041 TGAATATGCA GGGAAGGATA ACTACTGCTT GCAGATAAAC CCCGCTTCTT ACATCAATCC 
     5101 AGATCACCTG AAATATTTTC GTTTTATTGG CAGATTTATT GCCATGGCTC TGTTCCATGG 
     5161 GAAATTCATA GACACGGGTT TTTCTTTACC ATTCTATAAG CGTATCTTGA ACAAACCAGT 
     5221 TGGACTCAAG GATTTAGAAT CTATTGATCC AGAATTTTAC AATTCTCTCA TCTGGGTTAA 
     5281 GGAAAACAAT ATTGAGGAAT GTGATTTGGA AATGTACTTC TCCGTTGACA AAGAAATTCT 
     5341 AGGTGAAATT AAGAGTCATG ATCTGAAACC TAATGGTGGC AATATTCTTG TAACAGAAGA 
     5401 AAATAAAGAG GAATACATCA GAATGGTAGC TGAGTGGAGG TTGTCTCGAG GTGTTGAAGA 
     5461 ACAGACACAA GCTTTCTTTG AAGGCTTTAA TGAAATTCTT CCCCAGCAAT ATTTGCAATA 
     5521 CTTTGATGCA AAGGAATTAG AGGTCCTTTT ATGTGGAATG CAAGAGATTG ATTTGAATGA 
     5581 CTGGCAAAGA CATGCCATCT ACCGTCATTA TGCAAGGACC AGCAAACAAA TCATGTGGTT 
     5641 TTGGCAGTTT GTTAAAGAAA TTGATAATGA GAAGAGAATG AGACTTCTGC AGTTTGTTAC 
     5701 TGGAACCTGC CGATTGCCAG TAGGAGGATT TGCTGATCTC ATGGGGAGCA ATGGACCACA 
     5761 GAAATTATGC ATTGAAAAAG TTGGGAAAGA AAATTGGCTA CCCAGAAGTC ATACCTGTTT 
     5821 TAATCGCCTG GACCTGCCAC CATACAAGAG CTATGAGCAA CTGAAGGAAA AGCTGTTGTT 
     5881 TGCCATAGAA GAAACAGAAG GATTTGGACA AGAGACGCGT ACGCGGCCGC TCGAGATGAG 
     5941 CGGGGGCGAG GAGCTGTTCG CCGGCATCGT GCCCGTGCTG ATCGAGCTGG ACGGCGACGT 
     6001 GCACGGCCAC AAGTTCAGCG TGCGCGGCGA GGGCGAGGGC GACGCCGACT ACGGCAAGCT 
     6061 GGAGATCAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTGGTGAC 
     6121 CACCCTCTGC TACGGCATCC AGTGCTTCGC CCGCTACCCC GAGCACATGA AGATGAACGA 
     6181 CTTCTTCAAG AGCGCCATGC CCGAGGGCTA CATCCAGGAG CGCACCATCC AGTTCCAGGA 
     6241 CGACGGCAAG TACAAGACCC GCGGCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG 
     6301 CATCGAGCTG AAGGGCAAGG ACTTCAAGGA GGACGGCAAC ATCCTGGGCC ACAAGCTGGA 
     6361 GTACAGCTTC AACAGCCACA ACGTGTACAT CCGCCCCGAC AAGGCCAACA ACGGCCTGGA 
     6421 GGCTAACTTC AAGACCCGCC ACAACATCGA GGGCGGCGGC GTGCAGCTGG CCGACCACTA 
     6481 CCAGACCAAC GTGCCCCTGG GCGACGGCCC CGTGCTGATC CCCATCAACC ACTACCTGAG 
     6541 CACTCAGACC AAGATCAGCA AGGACCGCAA CGAGGCCCGC GACCACATGG TGCTCCTGGA 
     6601 GTCCTTCAGC GCCTGCTGCC ACACCCACGG CATGGACGAG CTGTACAGGT CCGGACTCAG 
     6661 ATAAGTTTAA ACGGCCGGCC GCGGTCTGTA CAAGTAGGAT TCGTCGAGGG ACCTAATAAC 
     6721 TTCGTATAGC ATACATTATA CGAAGTTATA CATGTTTAAG GGTTCCGGTT CCACTAGGTA 
     6781 CAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG 
     6841 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT 
     6901 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG 
     6961 TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT 
     7021 GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC 
     7081 GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC 
     7141 CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA 
     7201 TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC 
     7261 TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG 
     7321 GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG 
     7381 GCCGCCTCCC CGCATCGATA CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT 
     7441 CACAAGTAGC AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA 
     7501 GGAGGAGGTG GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC 
     7561 AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC 
     7621 CCAACGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA 
     7681 TTGGCAGAAC TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA 
     7741 CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG 
     7801 CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG 
     7861 GAGGTTTGAC AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC 
     7921 TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC 
     7981 ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT 
     8041 GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG 
     8101 CAGCATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     8161 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     8221 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     8281 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     8341 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     8401 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     8461 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     8521 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     8581 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     8641 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     8701 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     8761 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     8821 GGTCATGATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT 
     8881 CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC 
     8941 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC 
     9001 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA 
     9061 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA 
     9121 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT 
     9181 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC 
     9241 ACCAGCTCAC CGTCTTTCAT TGCCATACGG AACTCCGGAT GAGCATTCAT CAGGCGGGCA 
     9301 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG 
     9361 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC 
     9421 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT 
     9481 TTCTCCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     9541 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     9601 CCCCGAAAAG TGCCACCTGA C
//