LOCUS       RC220405                7566 bp    DNA     circular  SYN 
DEFINITION  CUL4B (Myc-DDK-tagged)-Human cullin 4B (CUL4B), transcript variant 2
ACCESSION   NM_001079872
VERSION     NM_001079872.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3723..3814 
                     /label="Myc-DDK"  
     misc_feature    3844..4429 
                     /label="PolyA signal"  
     misc_feature    3901..3920 
                     /label="Restriction Enzyme"  
     misc_feature    4705..5377 
                     /label="ColE1"  
     misc_feature    5807..6601 
                     /label="Kan/Neo"  
     misc_feature    6636..6938 
                     /label="SV40 ori"  
     misc_feature    7024..7501 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3705..3711 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3705 
                     /label="CUL4B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTCCAACA GGTTTTTCTT CCCCCAGTCC CTCAGCTGCT GCTGCTGCTC 
     1081 AGGAGGTCAG ATCTGCCACT GATGGTAATA CCAGCACCAC TCCGCCCACC TCTGCCAAGA 
     1141 AGAGAAAGTT AAACAGCAGC AGCAGTAGCA GCAGTAACAG TAGTAACGAG AGAGAAGACT 
     1201 TTGATTCCAC CTCTTCCTCC TCTTCCACTC CTCCTTTACA ACCCAGGGAT TCGGCATCCC 
     1261 CTTCAACCTC GTCCTTCTGC CTGGGGGTTT CAGTGGCTGC TTCCAGCCAC GTACCGATAC 
     1321 AGAAGAAGCT GCGTTTTGAA GACACCCTGG AGTTTGTAGG GTTTGATGCG AAGATGGCTG 
     1381 AGGAATCCTC CTCCTCCTCC TCCTCATCTT CACCAACTGC TGCAACATCT CAGCAGCAGC 
     1441 AACTTAAAAA TAAGAGTATA TTAATCTCTT CTGTGGCTTC GGTGCATCAT GCAAACGGCC 
     1501 TAGCCAAATC TTCTACCACC GTCTCTAGCT TTGCTAACAG CAAACCTGGC TCTGCTAAGA 
     1561 AGTTAGTGAT CAAGAACTTT AAAGATAAGC CTAAATTACC AGAAAACTAC ACAGATGAAA 
     1621 CCTGGCAAAA ACTGAAAGAA GCAGTGGAAG CTATTCAGAA TAGTACTTCA ATTAAGTACA 
     1681 ATTTAGAAGA ACTCTACCAG GCTGTAGAAA ATCTCTGTTC TTACAAGATT TCTGCAAACT 
     1741 TGTACAAACA GCTGAGACAG ATCTGCGAAG ATCACATCAA AGCACAGATT CATCAATTCA 
     1801 GAGAGGATTC ATTGGATAGC GTTCTTTTTT TAAAGAAGAT TGATAGATGC TGGCAAAACC 
     1861 ATTGCAGACA AATGATCATG ATCAGGAGCA TTTTTTTGTT TCTGGATAGA ACTTACGTTC 
     1921 TTCAGAATTC AATGCTACCC TCCATTTGGG ACATGGGACT GGAGTTATTT AGGGCTCATA 
     1981 TTATAAGTGA TCAGAAAGTG CAGAATAAGA CAATTGATGG CATTCTTCTC TTGATTGAGA 
     2041 GGGAAAGGAA TGGTGAAGCA ATTGATAGAA GTTTACTTCG AAGCCTTTTA AGCATGCTGT 
     2101 CTGATTTGCA AATTTATCAA GATTCTTTTG AACAACGATT TTTGGAAGAA ACTAACCGGC 
     2161 TCTATGCAGC TGAAGGCCAA AAATTAATGC AAGAAAGAGA GGTTCCTGAA TATCTACATC 
     2221 ATGTTAACAA ACGTCTAGAA GAAGAAGCAG ACAGACTTAT TACTTACTTA GATCAGACCA 
     2281 CCCAGAAGTC ATTAATTGCT ACTGTAGAAA AACAACTTCT AGGTGAACAC TTAACAGCAA 
     2341 TTCTTCAGAA AGGTTTAAAT AACCTCCTTG ATGAAAACCG AATTCAAGAT TTGTCTCTTC 
     2401 TGTATCAGCT CTTCAGTAGA GTTCGAGGTG GAGTTCAGGT TCTTTTGCAG CAGTGGATCG 
     2461 AATATATCAA GGCATTTGGC AGCACTATTG TAATTAATCC TGAAAAAGAT AAAACCATGG 
     2521 TTCAAGAATT GCTGGATTTT AAAGATAAGG TTGACCATAT AATTGATATC TGCTTTCTGA 
     2581 AGAATGAGAA ATTTATCAAT GCCATGAAAG AAGCATTTGA AACGTTCATT AACAAAAGAC 
     2641 CAAATAAACC AGCTGAACTT ATAGCTAAGT ATGTAGATTC AAAACTTCGT GCAGGCAACA 
     2701 AAGAAGCTAC AGATGAAGAA CTTGAGAAAA TGTTGGATAA AATTATGATC ATATTTAGAT 
     2761 TTATCTATGG CAAGGATGTT TTTGAGGCCT TCTATAAGAA AGATTTAGCC AAGCGCCTGT 
     2821 TAGTCGGAAA GAGTGCATCT GTAGATGCTG AAAAATCAAT GCTGTCCAAA CTTAAACATG 
     2881 AATGCGGAGC TGCTTTCACC AGCAAACTTG AAGGAATGTT TAAAGACATG GAACTTTCTA 
     2941 AAGACATCAT GATTCAGTTC AAACAGTATA TGCAGAATCA GAATGTTCCG GGAAATATTG 
     3001 AGTTAACTGT GAATATCCTG ACAATGGGCT ATTGGCCGAC ATATGTGCCT ATGGAAGTTC 
     3061 ATTTACCACC AGAGATGGTA AAACTTCAGG AGATTTTCAA GACATTTTAC CTAGGCAAAC 
     3121 ATAGTGGCAG GAAACTTCAG TGGCAGTCAA CCCTAGGACA CTGTGTGTTA AAAGCAGAAT 
     3181 TTAAAGAGGG TAAAAAGGAA CTCCAGGTCT CTCTTTTTCA AACACTGGTG CTGCTAATGT 
     3241 TTAATGAGGG AGAGGAGTTC AGTTTAGAAG AGATCAAGCA GGCAACTGGA ATAGAGGATG 
     3301 GAGAGTTAAG GAGAACACTG CAGTCATTAG CCTGTGGCAA AGCTAGAGTT CTGGCGAAAA 
     3361 ATCCAAAGGG CAAAGACATT GAAGATGGTG ACAAGTTCAT TTGTAATGAT GATTTCAAAC 
     3421 ATAAACTTTT CAGGATAAAG ATCAATCAAA TCCAGATGAA AGAAACGGTT GAAGAACAAG 
     3481 CAAGCACTAC AGAAAGAGTA TTTCAAGACA GACAGTATCA AATTGATGCT GCAATTGTTC 
     3541 GAATTATGAA GATGAGAAAG ACACTTAGCC ACAATCTCCT TGTTTCAGAA GTGTACAACC 
     3601 AGTTGAAATT TCCAGTAAAG CCTGCTGATC TTAAGAAGAG AATAGAATCT TTAATTGACC 
     3661 GGGACTACAT GGAAAGAGAT AAAGAAAATC CAAACCAGTA CAACTATATT GCAACGCGTA 
     3721 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3781 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3841 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3901 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3961 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4021 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4081 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4141 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4201 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4261 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4321 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4381 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4441 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4501 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4561 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4621 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4681 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4741 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4801 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4861 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4921 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4981 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5041 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5101 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5161 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5221 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5281 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5341 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5401 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5461 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5521 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5581 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5641 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5701 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5761 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5821 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5881 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5941 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6001 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6061 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6121 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6181 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6241 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6301 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6361 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6421 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6481 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6541 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6601 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6661 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6721 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6781 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6841 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6901 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6961 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7021 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7081 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7141 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7201 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7261 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7321 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7381 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7441 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7501 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7561 AATTTT
//