LOCUS       RC220367                8295 bp    DNA     circular  SYN 
DEFINITION  POLR1B (Myc-DDK-tagged)-Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1
ACCESSION   NM_019014
VERSION     NM_019014.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4450..4541 
                     /label="Myc-DDK"  
     misc_feature    4571..5156 
                     /label="PolyA signal"  
     misc_feature    4628..4647 
                     /label="Restriction Enzyme"  
     misc_feature    5432..6104 
                     /label="ColE1"  
     misc_feature    6534..7328 
                     /label="Kan/Neo"  
     misc_feature    7363..7665 
                     /label="SV40 ori"  
     misc_feature    7751..8228 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4425..4432 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4425 
                     /label="POLR1B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCCTGGC AGCCGGTGGC GGAACCTGCC CAGCGGGCCT AGCCTAAAGC 
     1081 ACTTGACTGA CCCCTCTTAT GGAATCCCGC GGGAACAGCA AAAGGCAGCG TTGCAGGAGC 
     1141 TGACGCGGGC GCACGTGGAG TCCTTCAACT ACGCTGTGCA CGAGGGTCTC GGCCTCGCGG 
     1201 TGCAGGCTAT ACCTCCCTTT GAATTTGCTT TCAAAGATGA GCGTATCTCT TTTACTATTC 
     1261 TGGATGCTGT TATCAGTCCA CCTACAGTTC CAAAAGGGAC CATCTGCAAA GAGGCCAATG 
     1321 TTTATCCAGC AGAATGCCGG GGCCGAAGGA GTACCTACCG TGGGAAGTTG ACAGCTGATA 
     1381 TCAACTGGGC AGTGAATGGA ATCTCAAAAG GAATCATTAA GCAGTTTCTT GGCTATGTTC 
     1441 CCATCATGGT GAAATCCAAG CTTTGCAACT TACGTAACCT TCCCCCACAA GCCCTCATTG 
     1501 AGCACCATGA GGAGGCAGAG GAAATGGGGG GCTATTTTAT AATCAATGGC ATTGAAAAAG 
     1561 TCATCCGAAT GTTGATTATG CCTCGGAGAA ATTTTCCCAT TGCAATGATA AGACCAAAAT 
     1621 GGAAAACCAG AGGGCCTGGT TATACTCAGT ATGGAGTTTC AATGCACTGT GTGAGGGAAG 
     1681 AACATTCCGC TGTCAATATG AACCTCCACT ACTTGGAAAA TGGCACAGTT ATGTTGAACT 
     1741 TTATTTACCG AAAAGAACTG TTCTTTCTTC CTTTGGGATT TGCACTTAAG GCACTTGTCA 
     1801 GCTTTTCTGA TTATCAGATC TTTCAGGAGC TCATCAAAGG AAAAGAGGAT GATTCTTTCC 
     1861 TTAGGAACTC TGTTTCTCAG ATGTTAAGGA TTGTAATGGA AGAGGGTTGT TCGACACAAA 
     1921 AACAGGTCCT TAACTACCTA GGTGAATGCT TCAGAGTAAA ACTCAATGTT CCTGACTGGT 
     1981 ACCCAAATGA GCAAGCTGCG GAGTTCCTGT TTAACCAGTG CATCTGTATC CACTTGAAAT 
     2041 CCAATACTGA AAAGTTTTAT ATGCTTTGTC TCATGACGCG AAAGCTCTTT GCTTTAGCCA 
     2101 AAGGAGAGTG CATGGAGGAC AATCCTGATA GTTTGGTGAA CCAGGAAGTC CTCACACCGG 
     2161 GTCAGCTCTT CCTTATGTTC CTGAAGGAAA AACTGGAAGG TTGGTTAGTG TCTATTAAAA 
     2221 TAGCTTTTGA TAAGAAGGCT CAGAAGACCA GTGTTTCCAT GAACACTGAC AATTTGATGA 
     2281 GGATTTTTAC AATGGGCATA GACCTTACAA AACCATTTGA ATACCTTTTT GCTACTGGGA 
     2341 ATCTGCGTTC TAAAACAGGT CTTGGCCTCC TACAAGATTC TGGACTTTGT GTTGTGGCTG 
     2401 ACAAGCTGAA CTTCATACGC TACCTCTCCC ATTTCCGCTG CGTGCACAGA GGGGCTGATT 
     2461 TTGCCAAGAT GAGGACCACC ACAGTACGCA GGCTGCTGCC AGAGTCCTGG GGCTTCCTTT 
     2521 GTCCCGTGCA TACCCCAGAC GGGGAGCCCT GTGGCCTGAT GAACCACCTA ACTGCCGTAT 
     2581 GTGAGGTTGT CACACAGTTT GTGTATACGG CATCTATTCC AGCTTTACTG TGCAACTTGG 
     2641 GGGTCACTCC CATTGATGGA GCTCCCCACC GATCATACAG TGAGTGCTAC CCTGTCCTGC 
     2701 TGGACGGTGT CATGGTTGGC TGGGTGGATA AGGATCTTGC TCCAGGCATC GCAGATTCTC 
     2761 TTCGTCATTT TAAGGTGTTG AGAGAGAAAA GAATTCCTCC CTGGATGGAA GTGGTCCTTA 
     2821 TACCCATGAC AGGAAAACCA AGTCTGTACC CAGGATTGTT CCTTTTTACC ACTCCTTGTA 
     2881 GACTGGTACG GCCTGTGCAG AACTTAGCAT TGGGCAAAGA AGAGCTAATT GGAACTATGG 
     2941 AACAGATCTT CATGAATGTC GCTATCTTTG AGGATGAAGT TTTTGCTGGA GTTACCACAC 
     3001 ACCAGGAACT CTTTCCACAC AGCCTGCTGA GTGTGATTGC CAACTTCATC CCTTTCTCTG 
     3061 ATCACAACCA GAGTCCACGG AACATGTACC AATGCCAGAT GGGTAAGCAA ACTATGGGCT 
     3121 TTCCACTTCT CACTTATCAA GACCGATCGG ATAACAAACT GTATCGTCTT CAGACTCCTC 
     3181 AGAGTCCCTT GGTGAGACCC TCCATGTATG ATTATTATGA CATGGATAAC TATCCAATTG 
     3241 GGACCAATGC CATCGTTGCT GTGATTTCTT ACACTGGCTA TGATATGGAA GATGCCATGA 
     3301 TTGTGAATAA GGCCTCTTGG GAACGAGGCT TTGCCCATGG AAGTGTCTAC AAGTCTGAGT 
     3361 TCATAGACCT CTCTGAAAAA ATTAAACAAG GAGATAGTAG CCTGGTGTTT GGCATCAAAC 
     3421 CTGGTGACCC ACGCGTTCTG CAGAAGTTAG ATGACGATGG ATTGCCGTTT ATAGGAGCAA 
     3481 AACTGCAGTA CGGAGATCCG TATTACAGCT ACCTCAACCT CAACACCGGG GAAAGTTTTG 
     3541 TGATGTACTA TAAGAGTAAA GAAAATTGTG TTGTGGATAA CATCAAAGTG TGCAGTAATG 
     3601 ACACTGGGAG TGGAAAATTC AAGTGTGTTT GCATCACTAT GAGAGTGCCT CGGAACCCAA 
     3661 CTATCGGAGA TAAATTTGCC AGTCGCCATG GGCAGAAGGG CATTTTAAGC AGATTGTGGC 
     3721 CGGCTGAGGA CATGCCTTTT ACTGAGAGTG GGATGGTCCC AGACATTCTG TTCAATCCCC 
     3781 ATGGTTTTCC ATCCCGCATG ACCATTGGGA TGTTAATTGA GAGTATGGCC GGGAAGTCTG 
     3841 CAGCTTTGCA TGGTCTCTGC CATGATGCTA CACCCTTCAT CTTCTCAGAG GAGAACTCGG 
     3901 CCTTAGAATA CTTTGGTGAG ATGTTAAAGG CTGCTGGCTA CAATTTCTAT GGCACCGAGA 
     3961 GGTTATATAG TGGCATCAGT GGGCTAGAAC TGGAAGCAGA CATCTTCATA GGAGTGGTTT 
     4021 ATTATCAGCG CTTACGCCAT ATGGTCTCAG ACAAATTTCA AGTAAGGACA ACTGGAGCCC 
     4081 GAGACAGAGT CACCAACCAG CCTATTGGGG GAAGAAATGT CCAGGGTGGA ATCCGTTTTG 
     4141 GGGAGATGGA ACGGGATGCG CTTTTAGCTC ATGGTACATC TTTTCTCCTT CATGACCGCC 
     4201 TCTTCAACTG CTCAGATCGG TCGGTAGCCC ATGTGTGTGT GAAGTGTGGC AGTTTACTCT 
     4261 CTCCACTGTT GGAGAAGCCA CCCCCTTCTT GGTCTGCCAT GCGCAACAGA AAATACAACT 
     4321 GTACTCTGTG TAGTCGCAGT GACACTATCG ATACTGTTTC TGTGCCTTAT GTTTTTCGGT 
     4381 ATTTTGTAGC TGAACTGGCA GCTATGAACA TCAAAGTGAA ACTGGATGTT GTTAGCGGAC 
     4441 CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4501 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4561 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4621 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4681 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4741 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4801 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4861 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4921 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4981 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5041 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5101 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5161 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5221 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5281 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5341 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5401 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5461 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5521 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5581 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5641 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5701 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5761 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5821 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5881 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5941 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6001 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6061 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6121 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6181 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6241 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6301 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6361 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6421 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6481 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6541 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6601 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6661 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6721 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6781 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6841 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6901 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6961 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7021 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7081 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7141 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7201 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7261 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7321 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7381 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7441 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7501 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7561 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7621 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7681 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7741 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7801 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7861 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7921 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7981 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8041 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8101 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8161 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8221 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8281 TTTAACGCGA ATTTT
//