LOCUS       RC220336                8652 bp    DNA     circular  SYN 
DEFINITION  PPFIA2 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (PPFIA2), transcript variant 1
ACCESSION   NM_003625
VERSION     NM_003625.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4809..4900 
                     /label="Myc-DDK"  
     misc_feature    4930..5515 
                     /label="PolyA signal"  
     misc_feature    4987..5006 
                     /label="Restriction Enzyme"  
     misc_feature    5791..6463 
                     /label="ColE1"  
     misc_feature    6893..7687 
                     /label="Kan/Neo"  
     misc_feature    7722..8024 
                     /label="SV40 ori"  
     misc_feature    8110..8587 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4791..4797 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4791 
                     /label="PPFIA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGTGAA GTGATGCCCA CGATTAATGA GGACACCCCA ATGAGCCAAA 
     1081 GGGGGTCCCA AAGCAGTGGC TCGGACTCAG ACTCCCATTT TGAGCAGCTG ATGGTGAATA 
     1141 TGCTAGATGA AAGGGATCGT CTTCTAGACA CCCTTCGGGA GACCCAGGAA AGCCTCTCAC 
     1201 TTGCCCAGCA AAGACTTCAG GATGTCATCT ATGACCGAGA CTCACTCCAG AGACAGCTCA 
     1261 ATTCAGCCCT GCCACAGGAT ATCGAATCCC TAACAGGAGG GCTGGCTGGT TCTAAGGGGG 
     1321 CTGATCCACC GGAATTTGCT GCACTGACAA AAGAATTAAA TGCCTGCAGG GAACAACTTC 
     1381 TAGAAAAGGA AGAAGAAATC TCTGAACTTA AAGCTGAAAG AAACAACACA AGACTATTAC 
     1441 TGGAGCATTT GGAGTGCCTT GTGTCACGAC ATGAAAGATC ACTAAGAATG ACGGTGGTAA 
     1501 AACGGCAAGC CCAGTCTCCC TCAGGAGTAT CCAGTGAAGT TGAAGTTCTC AAGGCACTGA 
     1561 AATCTTTGTT TGAGCACCAC AAGGCCTTGG ATGAAAAGGT AAGGGAGCGA CTGAGGGTTT 
     1621 CTTTAGAAAG AGTCTCTGCA CTGGAAGAAG AACTAGCTGC TGCTAATCAG GAGATTGTTG 
     1681 CCTTGCGTGA ACAAAATGTT CATATACAAA GAAAAATGGC ATCAAGCGAG GGATCCACAG 
     1741 AGTCAGAACA TCTTGAAGGG ATGGAACCTG GACAGAAAGT CCATGAGAAG CGTTTGTCCA 
     1801 ATGGTTCTAT AGACTCAACC GATGAAACTA GTCAAATAGT TGAACTACAA GAATTGCTTG 
     1861 AAAAGCAAAA CTATGAAATG GCCCAGATGA AAGAACGTTT AGCAGCCCTT TCTTCCCGAG 
     1921 TGGGAGAGGT GGAACAGGAA GCAGAGACAG CAAGAAAGGA TCTCATTAAA ACAGAAGAAA 
     1981 TGAACACCAA GTATCAAAGG GACATTAGGG AGGCCATGGC ACAAAAGGAA GATATGGAAG 
     2041 AAAGAATTAC AACCCTTGAA AAGCGTTACC TCAGTGCTCA GAGAGAATCT ACCTCCATAC 
     2101 ATGACATGAA TGATAAACTA GAAAATGAGT TAGCAAATAA AGAAGCTATC CTGCGGCAGA 
     2161 TGGAAGAGAA AAACAGACAG TTACAAGAAC GTCTTGAGCT AGCTGAACAA AAGTTGCAGC 
     2221 AGACCATGAG AAAGGCTGAA ACCTTGCCTG AAGTAGAGGC TGAACTGGCT CAGAGAATTG 
     2281 CAGCCCTAAC CAAGGCTGAA GAGAGACATG GAAATATTGA AGAACGTATG AGACATTTAG 
     2341 AGGGTCAACT TGAAGAGAAG AATCAAGAAC TTCAAAGAGC TAGGCAAAGA GAGAAAATGA 
     2401 ATGAGGAGCA TAACAAGAGA TTATCGGATA CGGTTGATAG ACTTCTGACT GAATCCAATG 
     2461 AACGCCTACA ACTACACTTA AAGGAAAGAA TGGCTGCTCT AGAAGAAAAG AATGTTTTAA 
     2521 TTCAAGAATC AGAAACTTTC AGAAAGAATC TTGAAGAATC TTTACATGAT AAGGAAAGAT 
     2581 TAGCAGAAGA AATTGAAAAG CTGAGATCTG AACTTGACCA ATTGAAAATG AGAACTGGCT 
     2641 CTTTAATTGA ACCCACAATA CCAAGAACTC ATCTAGACAC CTCAGCTGAG TTGCGGTACT 
     2701 CAGTGGGATC CCTAGTGGAC AGCCAGTCTG ATTACAGAAC AACTAAAGTA ATAAGAAGAC 
     2761 CAAGGAGAGG CCGCATGGGT GTGCGAAGAG ATGAGCCAAA GGTGAAATCT CTTGGGGATC 
     2821 ACGAGTGGAA TAGAACTCAA CAGATTGGAG TACTAAGCAG CCACCCTTTT GAAAGTGACA 
     2881 CTGAAATGTC TGATATTGAT GATGATGACA GAGAAACAAT TTTTAGCTCA ATGGATCTTC 
     2941 TCTCTCCAAG TGGTCATTCC GATGCCCAGA CGCTAGCCAT GATGCTTCAG GAACAATTGG 
     3001 ATGCCATCAA CAAAGAAATC AGGCTAATTC AGGAAGAAAA AGAATCTACA GAGTTGCGTG 
     3061 CTGAAGAAAT TGAAAATAGA GTGGCTAGTG TGAGCCTCGA AGGCCTGAAT TTGGCAAGGG 
     3121 TCCACCCAGG TACCTCCATT ACTGCCTCTG TTACAGCTTC ATCGCTGGCC AGTTCATCTC 
     3181 CCCCCAGTGG ACACTCAACT CCAAAGCTCA CCCCTCGAAG CCCTGCCAGG GAAATGGATC 
     3241 GGATGGGAGT CATGACACTG CCAAGTGATC TGAGGAAACA TCGGAGAAAG ATTGCAGTTG 
     3301 TGGAAGAAGA TGGTCGAGAG GACAAAGCAA CAATTAAATG TGAAACTTCT CCTCCTCCTA 
     3361 CCCCTAGAGC CCTCAGAATG ACTCACACTC TCCCTTCTTC CTACCACAAT GATGCTCGAA 
     3421 GTAGTTTATC TGTCTCTCTT GAGCCAGAAA GCCTCGGGCT TGGTAGTGCC AACAGCAGCC 
     3481 AAGACTCTCT TCACAAAGCC CCCAAGAAGA AAGGAATCAA GTCTTCAATA GGACGTTTGT 
     3541 TTGGTAAAAA AGAAAAAGCT CGACTTGGGC AGCTCCGAGG CTTTATGGAG ACTGAAGCTG 
     3601 CAGCTCAGGA GTCCCTGGGG TTAGGCAAAC TCGGAACTCA AGCTGAGAAG GATCGAAGAC 
     3661 TAAAGAAAAA GCATGAACTT CTTGAAGAAG CTCGGAGAAA GGGATTACCT TTTGCCCAGT 
     3721 GGGATGGGCC AACTGTGGTC GCATGGCTAG AGCTTTGGTT GGGAATGCCT GCGTGGTACG 
     3781 TGGCAGCCTG CCGAGCCAAC GTGAAGAGTG GTGCCATCAT GTCTGCTTTA TCTGACACTG 
     3841 AGATCCAGAG AGAAATTGGA ATCAGCAATC CACTGCATCG CTTAAAACTT CGATTAGCAA 
     3901 TCCAGGAGAT GGTTTCCCTA ACAAGTCCTT CAGCTCCTCC AACATCTCGA ACTCCTTCAG 
     3961 GCAACGTTTG GGTGACTCAT GAAGAAATGG AAAATCTTGC AGCTCCAGCA AAAACGAAAG 
     4021 AATCTGAGGA AGGAAGCTGG GCCCAGTGTC CGGTTTTTCT ACAGACCCTG GCTTATGGAG 
     4081 ATATGAATCA TGAGTGGATT GGAAATGAAT GGCTTCCCAG CTTGGGGTTA CCTCAGTACA 
     4141 GAAGTTACTT TATGGAATGC TTGGTAGATG CAAGAATGTT AGATCACCTA ACAAAAAAAG 
     4201 ATCTCCGTGT CCATTTAAAA ATGGTGGATA GTTTCCATCG AACAAGTTTA CAATATGGAA 
     4261 TTATGTGCTT AAAGAGGTTG AATTATGACA GAAAAGAACT AGAAAGAAGA CGGGAAGCAA 
     4321 GCCAACATGA AATAAAAGAC GTGTTGGTGT GGAGCAATGA CCGAGTTATT CGCTGGATAC 
     4381 AAGCAATTGG ACTTCGAGAA TATGCAAATA ATATACTTGA GAGCGGTGTG CATGGCTCAC 
     4441 TTATAGCCCT GGATGAAAAC TTTGACTACA GCAGCTTAGC TTTATTATTA CAGATTCCAA 
     4501 CACAGAACAC CCAGGCAAGG CAGATTCTTG AAAGAGAATA CAATAACCTC TTGGCCCTGG 
     4561 GAACTGAAAG GCGACTGGAT GAAAGTGATG ACAAGAACTT CAGACGTGGA TCAACCTGGA 
     4621 GAAGGCAGTT TCCTCCTCGT GAAGTACATG GAATCAGCAT GATGCCTGGG TCCTCAGAAA 
     4681 CATTACCAGC TGGATTTAGG TTAACCACAA CCTCTGGGCA GTCAAGAAAA ATGACAACAG 
     4741 ATGTTGCTTC ATCAAGACTG CAGAGGTTAG ACAACTCCAC TGTTCGCACA TACTCATGTA 
     4801 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4861 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4921 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4981 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5041 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5101 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5161 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5221 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5281 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5341 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5401 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5461 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5521 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5581 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5641 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5701 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5761 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5821 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5881 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5941 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6001 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6061 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6121 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6181 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6241 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6301 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6361 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6421 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6481 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6541 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6601 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6661 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6721 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6781 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6841 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6901 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6961 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7021 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7081 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7141 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7201 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7261 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7321 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7381 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7441 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7501 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7561 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7621 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7681 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7741 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7801 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7861 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8041 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8101 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8161 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8221 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8281 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8341 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8401 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8461 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8521 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8581 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8641 AACGCGAATT TT
//