LOCUS       RC220320                9162 bp    DNA     circular  SYN 
DEFINITION  ADAMTS13 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13), transcript variant 1
ACCESSION   NM_139025
VERSION     NM_139025.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5319..5410 
                     /label="Myc-DDK"  
     misc_feature    5440..6025 
                     /label="PolyA signal"  
     misc_feature    5497..5516 
                     /label="Restriction Enzyme"  
     misc_feature    6301..6973 
                     /label="ColE1"  
     misc_feature    7403..8197 
                     /label="Kan/Neo"  
     misc_feature    8232..8534 
                     /label="SV40 ori"  
     misc_feature    8620..9097 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5301..5307 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5301 
                     /label="ADAMTS13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACCAGCGT CACCCCCGGG CAAGATGCCC TCCCCTCTGT GTGGCCGGAA 
     1081 TCCTTGCCTG TGGCTTTCTC CTGGGCTGCT GGGGACCCTC CCATTTCCAG CAGAGTTGTC 
     1141 TTCAGGCTTT GGAGCCACAG GCCGTGTCTT CTTACTTGAG CCCTGGTGCT CCCTTAAAAG 
     1201 GCCGCCCTCC TTCCCCTGGC TTCCAGAGGC AGAGGCAGAG GCAGAGGCGG GCTGCAGGCG 
     1261 GCATCCTACA CCTGGAGCTG CTGGTGGCCG TGGGCCCCGA TGTCTTCCAG GCTCACCAGG 
     1321 AGGACACAGA GCGCTATGTG CTCACCAACC TCAACATCGG GGCAGAACTG CTTCGGGACC 
     1381 CGTCCCTGGG GGCTCAGTTT CGGGTGCACC TGGTGAAGAT GGTCATTCTG ACAGAGCCTG 
     1441 AGGGTGCCCC AAATATCACA GCCAACCTCA CCTCGTCCCT GCTGAGCGTC TGTGGGTGGA 
     1501 GCCAGACCAT CAACCCTGAG GACGACACGG ATCCTGGCCA TGCTGACCTG GTCCTCTATA 
     1561 TCACTAGGTT TGACCTGGAG TTGCCTGATG GTAACCGGCA GGTGCGGGGC GTCACCCAGC 
     1621 TGGGCGGTGC CTGCTCCCCA ACCTGGAGCT GCCTCATTAC CGAGGACACT GGCTTCGACC 
     1681 TGGGAGTCAC CATTGCCCAT GAGATTGGGC ACAGCTTCGG CCTGGAGCAC GACGGCGCGC 
     1741 CCGGCAGCGG CTGCGGCCCC AGCGGACACG TGATGGCTTC GGACGGCGCC GCGCCCCGCG 
     1801 CCGGCCTCGC CTGGTCCCCC TGCAGCCGCC GGCAGCTGCT GAGCCTGCTC AGCGCAGGAC 
     1861 GGGCGCGCTG CGTGTGGGAC CCGCCGCGGC CTCAACCCGG GTCCGCGGGG CACCCGCCGG 
     1921 ATGCGCAGCC TGGCCTCTAC TACAGCGCCA ACGAGCAGTG CCGCGTGGCC TTCGGCCCCA 
     1981 AGGCTGTCGC CTGCACCTTC GCCAGGGAGC ACCTGGATAT GTGCCAGGCC CTCTCCTGCC 
     2041 ACACAGACCC GCTGGACCAA AGCAGCTGCA GCCGCCTCCT CGTTCCTCTC CTGGATGGGA 
     2101 CAGAATGTGG CGTGGAGAAG TGGTGCTCCA AGGGTCGCTG CCGCTCCCTG GTGGAGCTGA 
     2161 CCCCCATAGC AGCAGTGCAT GGGCGCTGGT CTAGCTGGGG TCCCCGAAGT CCTTGCTCCC 
     2221 GCTCCTGCGG AGGAGGTGTG GTCACCAGGA GGCGGCAGTG CAACAACCCC AGACCTGCCT 
     2281 TTGGGGGGCG TGCATGTGTT GGTGCTGACC TCCAGGCCGA GATGTGCAAC ACTCAGGCCT 
     2341 GCGAGAAGAC CCAGCTGGAG TTCATGTCGC AACAGTGCGC CAGGACCGAC GGCCAGCCGC 
     2401 TGCGCTCCTC CCCTGGCGGC GCCTCCTTCT ACCACTGGGG TGCTGCTGTA CCACACAGCC 
     2461 AAGGGGATGC TCTGTGCAGA CACATGTGCC GGGCCATTGG CGAGAGCTTC ATCATGAAGC 
     2521 GTGGAGACAG CTTCCTCGAT GGGACCCGGT GTATGCCAAG TGGCCCCCGG GAGGACGGGA 
     2581 CCCTGAGCCT GTGTGTGTCG GGCAGCTGCA GGACATTTGG CTGTGATGGT AGGATGGACT 
     2641 CCCAGCAGGT ATGGGACAGG TGCCAGGTGT GTGGTGGGGA CAACAGCACG TGCAGCCCAC 
     2701 GGAAGGGCTC TTTCACAGCT GGCAGAGCGA GAGAATATGT CACATTTCTG ACAGTTACCC 
     2761 CCAACCTGAC CAGTGTCTAC ATTGCCAACC ACAGGCCTCT CTTCACACAC TTGGCGGTGA 
     2821 GGATCGGAGG GCGCTATGTC GTGGCTGGGA AGATGAGCAT CTCCCCTAAC ACCACCTACC 
     2881 CCTCCCTCCT GGAGGATGGT CGTGTCGAGT ACAGAGTGGC CCTCACCGAG GACCGGCTGC 
     2941 CCCGCCTGGA GGAGATCCGC ATCTGGGGAC CCCTCCAGGA AGATGCTGAC ATCCAGGTTT 
     3001 ACAGGCGGTA TGGCGAGGAG TATGGCAACC TCACCCGCCC AGACATCACC TTCACCTACT 
     3061 TCCAGCCTAA GCCACGGCAG GCCTGGGTGT GGGCCGCTGT GCGTGGGCCC TGCTCGGTGA 
     3121 GCTGTGGGGC AGGGCTGCGC TGGGTAAACT ACAGCTGCCT GGACCAGGCC AGGAAGGAGT 
     3181 TGGTGGAGAC TGTCCAGTGC CAAGGGAGCC AGCAGCCACC AGCGTGGCCA GAGGCCTGCG 
     3241 TGCTCGAACC CTGCCCTCCC TACTGGGCGG TGGGAGACTT CGGCCCATGC AGCGCCTCCT 
     3301 GTGGGGGCGG CCTGCGGGAG CGGCCAGTGC GCTGCGTGGA GGCCCAGGGC AGCCTCCTGA 
     3361 AGACATTGCC CCCAGCCCGG TGCAGAGCAG GGGCCCAGCA GCCAGCTGTG GCGCTGGAAA 
     3421 CCTGCAACCC CCAGCCCTGC CCTGCCAGGT GGGAGGTGTC AGAGCCCAGC TCATGCACAT 
     3481 CAGCTGGTGG AGCAGGCCTG GCCTTGGAGA ACGAGACCTG TGTGCCAGGG GCAGATGGCC 
     3541 TGGAGGCTCC AGTGACTGAG GGGCCTGGCT CCGTAGATGA GAAGCTGCCT GCCCCTGAGC 
     3601 CCTGTGTCGG GATGTCATGT CCTCCAGGCT GGGGCCATCT GGATGCCACC TCTGCAGGGG 
     3661 AGAAGGCTCC CTCCCCATGG GGCAGCATCA GGACGGGGGC TCAAGCTGCA CACGTGTGGA 
     3721 CCCCTGCGGC AGGGTCGTGC TCCGTCTCCT GCGGGCGAGG TCTGATGGAG CTGCGTTTCC 
     3781 TGTGCATGGA CTCTGCCCTC AGGGTGCCTG TCCAGGAAGA GCTGTGTGGC CTGGCAAGCA 
     3841 AGCCTGGGAG CCGGCGGGAG GTCTGCCAGG CTGTCCCGTG CCCTGCTCGG TGGCAGTACA 
     3901 AGCTGGCGGC CTGCAGCGTG AGCTGTGGGA GAGGGGTCGT GCGGAGGATC CTGTATTGTG 
     3961 CCCGGGCCCA TGGGGAGGAC GATGGTGAGG AGATCCTGTT GGACACCCAG TGCCAGGGGC 
     4021 TGCCTCGCCC GGAACCCCAG GAGGCCTGCA GCCTGGAGCC CTGCCCACCT AGGTGGAAAG 
     4081 TCATGTCCCT TGGCCCATGT TCGGCCAGCT GTGGCCTTGG CACTGCTAGA CGCTCGGTGG 
     4141 CCTGTGTGCA GCTCGACCAA GGCCAGGACG TGGAGGTGGA CGAGGCGGCC TGTGCGGCGC 
     4201 TGGTGCGGCC CGAGGCCAGT GTCCCCTGTC TCATTGCCGA CTGCACCTAC CGCTGGCATG 
     4261 TTGGCACCTG GATGGAGTGC TCTGTTTCCT GTGGGGATGG CATCCAGCGC CGGCGTGACA 
     4321 CCTGCCTCGG ACCCCAGGCC CAGGCGCCTG TGCCAGCTGA TTTCTGCCAG CACTTGCCCA 
     4381 AGCCGGTGAC TGTGCGTGGC TGCTGGGCTG GGCCCTGTGT GGGACAGGGT ACGCCCAGCC 
     4441 TGGTGCCCCA CGAAGAAGCC GCTGCTCCAG GACGGACCAC AGCCACCCCT GCTGGTGCCT 
     4501 CCCTGGAGTG GTCCCAGGCC CGGGGCCTGC TCTTCTCCCC GGCTCCCCAG CCTCGGCGGC 
     4561 TCCTGCCCGG GCCCCAGGAA AACTCAGTGC AGTCCAGTGC CTGTGGCAGG CAGCACCTTG 
     4621 AGCCAACAGG AACCATTGAC ATGCGAGGCC CAGGGCAGGC AGACTGTGCA GTGGCCATTG 
     4681 GGCGGCCCCT CGGGGAGGTG GTGACCCTCC GCGTCCTTGA GAGTTCTCTC AACTGCAGTG 
     4741 CGGGGGACAT GTTGCTGCTT TGGGGCCGGC TCACCTGGAG GAAGATGTGC AGGAAGCTGT 
     4801 TGGACATGAC TTTCAGCTCC AAGACCAACA CGCTGGTGGT GAGGCAGCGC TGCGGGCGGC 
     4861 CAGGAGGTGG GGTGCTGCTG CGGTATGGGA GCCAGCTTGC TCCTGAAACC TTCTACAGAG 
     4921 AATGTGACAT GCAGCTCTTT GGGCCCTGGG GTGAAATCGT GAGCCCCTCG CTGAGTCCAG 
     4981 CCACGAGTAA TGCAGGGGGC TGCCGGCTCT TCATTAATGT GGCTCCGCAC GCACGGATTG 
     5041 CCATCCATGC CCTGGCCACC AACATGGGCG CTGGGACCGA GGGAGCCAAT GCCAGCTACA 
     5101 TCTTGATCCG GGACACCCAC AGCTTGAGGA CCACAGCGTT CCATGGGCAG CAGGTGCTCT 
     5161 ACTGGGAGTC AGAGAGCAGC CAGGCTGAGA TGGAGTTCAG CGAGGGCTTC CTGAAGGCTC 
     5221 AGGCCAGCCT GCGGGGCCAG TACTGGACCC TCCAATCATG GGTACCGGAG ATGCAGGACC 
     5281 CTCAGTCCTG GAAGGGAAAG GAAGGAACCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5341 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5401 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5461 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5521 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5581 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5641 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5701 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5761 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5821 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5881 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5941 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6001 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6061 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6121 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6181 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6241 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6301 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6361 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6421 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6481 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6541 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6601 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6661 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6721 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6781 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6841 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6901 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6961 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7021 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7081 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7141 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7201 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7261 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7321 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7381 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7441 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7501 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7561 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7621 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7681 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7741 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7801 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7861 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7921 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7981 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8041 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8101 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8161 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8221 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8281 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8341 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8401 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8461 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8521 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8581 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8641 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8701 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8761 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8821 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8881 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8941 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9001 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9061 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9121 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//