LOCUS       RC220304                7680 bp    DNA     circular  SYN 
DEFINITION  PDE11A (Myc-DDK-tagged)-Human phosphodiesterase 11A (PDE11A), transcript variant 4
ACCESSION   NM_016953
VERSION     NM_016953.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3837..3928 
                     /label="Myc-DDK"  
     misc_feature    3958..4543 
                     /label="PolyA signal"  
     misc_feature    4015..4034 
                     /label="Restriction Enzyme"  
     misc_feature    4819..5491 
                     /label="ColE1"  
     misc_feature    5921..6715 
                     /label="Kan/Neo"  
     misc_feature    6750..7052 
                     /label="SV40 ori"  
     misc_feature    7138..7615 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3819..3825 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3819 
                     /label="PDE11A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCCTCC CGCCTGGACT TTGGGGAGGT GGAAACTTTC CTGGACAGGC 
     1081 ACCCAGAGTT GTTTGAAGAT TACTTGATGC GGAAGGGGAA GCAGGAGATG GTTGAAAAGT 
     1141 GGCTGCAGAG GCACAGTCAG GGTCAGGGGG CTTTAGGTCC AAGGCCCTCT TTGGCTGGTA 
     1201 CCAGCAGCTT GGCTCACAGC ACCTGCAGAG GTGGCAGCAG CGTTGGTGGT GGCACTGGAC 
     1261 CAAATGGCTC TGCCCACAGC CAGCCCCTTC CCGGTGGCGG GGACTGTGGT GGGGTTCCCT 
     1321 TGAGTCCCAG CTGGGCCGGT GGCAGCAGGG GCGATGGGAA CCTGCAGCGG AGAGCTTCTC 
     1381 AGAAAGAGCT AAGGAAGAGT TTTGCCCGCT CCAAGGCCAT CCACGTGAAC AGGACCTACG 
     1441 ATGAACAGGT GACCTCCCGG GCTCAGGAAC CCCTGAGTAG TGTACGACGG AGGGCACTTC 
     1501 TCCGGAAGGC AAGCTCCCTG CCCCCCACCA CAGCCCATAT TCTCAGTGCG CTGCTGGAAT 
     1561 CGAGAGTGAA TCTGCCTCAG TATCCCCCTA CAGCCATCGA CTACAAGTGC CATCTGAAAA 
     1621 AGCATAATGA GCGTCAGTTC TTTCTGGAAT TGGTCAAAGA TATCTCCAAT GACCTTGACC 
     1681 TCACCAGCCT GAGCTACAAG ATTCTCATCT TTGTCTGCCT TATGGTGGAT GCTGACCGCT 
     1741 GCTCTCTTTT CCTGGTGGAA GGGGCAGCTG CTGGCAAGAA GACCTTGGTC TCCAAATTCT 
     1801 TTGATGTGCA TGCAGGAACC CCTCTGCTGC CTTGCAGCAG CACAGAGAAC TCAAATGAGG 
     1861 TGCAGGTCCC CTGGGGCAAA GGTATCATTG GCTATGTCGG GGAGCATGGA GAAACGGTCA 
     1921 ACATTCCTGA TGCCTACCAG GATCGACGAT TCAATGATGA AATCGACAAG CTAACTGGAT 
     1981 ACAAGACAAA ATCATTATTG TGCATGCCTA TCCGAAGCAG TGATGGTGAG ATTATTGGTG 
     2041 TGGCCCAAGC GATAAATAAG ATTCCTGAAG GAGCTCCATT TACTGAAGAT GATGAAAAAG 
     2101 TTATGCAGAT GTATCTTCCA TTTTGTGGAA TCGCCATATC TAACGCTCAG CTCTTTGCTG 
     2161 CCTCAAGGAA AGAATATGAA AGAAGCAGAG CTTTGCTAGA GGTGGTTAAT GACCTCTTTG 
     2221 AAGAACAGAC TGACCTGGAG AAAATTGTCA AGAAAATAAT GCATCGGGCC CAAACTCTGC 
     2281 TGAAATGTGA ACGCTGTTCT GTTTTACTCC TAGAGGACAT CGAATCACCA GTGGTGAAAT 
     2341 TTACCAAATC CTTTGAATTG ATGTCCCCAA AGTGCAGTGC TGATGCTGAG AACAGTTTCA 
     2401 AAGAAAGCAT GGAGAAATCA TCATACTCCG ACTGGCTAAT AAATAACAGC ATTGCTGAGC 
     2461 TGGTTGCTTC AACAGGCCTT CCAGTGAACA TCAGTGATGC CTACCAGGAT CCGCGCTTTG 
     2521 ATGCAGAGGC AGACCAGATA TCTGGTTTTC ACATAAGATC TGTTCTTTGT GTCCCTATTT 
     2581 GGAATAGCAA CCACCAAATA ATTGGAGTGG CTCAAGTGTT AAACAGACTT GATGGGAAAC 
     2641 CTTTTGATGA TGCAGATCAA CGACTTTTTG AGGCTTTTGT CATCTTTTGT GGACTTGGCA 
     2701 TCAACAACAC AATTATGTAT GATCAAGTGA AGAAGTCCTG GGCCAAGCAG TCTGTGGCTC 
     2761 TTGATGTGCT ATCATACCAT GCAACATGTT CAAAAGCTGA AGTTGACAAG TTTAAGGCAG 
     2821 CCAACATCCC TCTGGTGTCA GAACTTGCCA TCGATGACAT TCATTTTGAT GACTTTTCTC 
     2881 TCGACGTTGA TGCCATGATC ACAGCTGCTC TCCGGATGTT CATGGAGCTG GGGATGGTAC 
     2941 AGAAATTTAA AATTGACTAT GAGACACTGT GTAGGTGGCT TTTGACAGTG AGGAAAAACT 
     3001 ATCGGATGGT TCTATACCAC AACTGGAGAC ATGCCTTCAA CGTGTGTCAG CTGATGTTCG 
     3061 CGATGTTAAC CACTGCTGGG TTTCAAGACA TTCTGACCGA GGTGGAAATT TTAGCGGTGA 
     3121 TTGTGGGATG CCTGTGTCAT GACCTCGACC ACAGGGGAAC CAACAATGCC TTCCAAGCTA 
     3181 AGAGTGGCTC TGCCCTGGCC CAACTCTATG GAACCTCTGC TACCTTGGAG CATCACCATT 
     3241 TCAACCACGC CGTGATGATC CTTCAAAGTG AGGGTCACAA TATCTTTGCT AACCTGTCCT 
     3301 CCAAGGAATA TAGTGACCTT ATGCAGCTTT TGAAGCAGTC AATATTGGCA ACAGACCTCA 
     3361 CGCTGTACTT TGAGAGGAGA ACTGAATTCT TTGAACTTGT CAGTAAAGGA GAATACGATT 
     3421 GGAACATCAA AAACCATCGT GATATATTTC GATCAATGTT AATGACAGCC TGTGACCTTG 
     3481 GAGCCGTGAC CAAACCGTGG GAGATCTCCA GACAGGTGGC AGAACTTGTA ACCAGTGAGT 
     3541 TCTTCGAACA AGGAGATCGG GAGAGATTAG AGCTCAAACT CACTCCTTCA GCAATTTTTG 
     3601 ATCGGAACCG GAAGGATGAA CTGCCTCGGT TGCAACTGGA GTGGATTGAT AGCATCTGCA 
     3661 TGCCTTTGTA TCAGGCACTG GTGAAGGTCA ACGTGAAACT GAAGCCGATG CTAGATTCAG 
     3721 TAGCTACAAA CAGAAGTAAG TGGGAAGAGC TACACCAAAA ACGACTGCTG GCCTCAACTG 
     3781 CCTCATCCTC CCCTGCCAGT GTTATGGTAG CCAAGGAAGA CAGGAACACG CGTACGCGGC 
     3841 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3901 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3961 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4021 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4081 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4141 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4201 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4261 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4321 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4381 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4441 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4501 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4561 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4621 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4681 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4741 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4801 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4861 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4921 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4981 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5041 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5101 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5161 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5221 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5281 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5341 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5401 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5461 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5521 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5581 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5641 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5701 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5761 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5821 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5881 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5941 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6001 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6061 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6121 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6181 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6241 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6301 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6361 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6421 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6481 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6541 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6601 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6661 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6721 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6781 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6841 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6901 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6961 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7021 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7081 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7141 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7201 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7261 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7321 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7381 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7441 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7501 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7561 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7621 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
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