LOCUS       RC220279                8649 bp    DNA     circular  SYN 
DEFINITION  MAGI1 (Myc-DDK-tagged)-Human membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant 2
ACCESSION   NM_004742
VERSION     NM_004742.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4806..4897 
                     /label="Myc-DDK"  
     misc_feature    4927..5512 
                     /label="PolyA signal"  
     misc_feature    4984..5003 
                     /label="Restriction Enzyme"  
     misc_feature    5788..6460 
                     /label="ColE1"  
     misc_feature    6890..7684 
                     /label="Kan/Neo"  
     misc_feature    7719..8021 
                     /label="SV40 ori"  
     misc_feature    8107..8584 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4788..4794 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4788 
                     /label="MAGI1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAAAGTG ATCCAGAAGA AGAACCACTG GACTAGCAGG GTTCACGAAT 
     1081 GCACCGTGAA GCGGGGACCC CAGGGCGAGC TGGGGGTGAC GGTGCTGGGA GGCGCGGAGC 
     1141 ACGGGGAGTT TCCGTACGTC GGAGCGGTGG CGGCGGTCGA GGCAGCGGGG CTTCCCGGCG 
     1201 GCGGCGAGGG CCCGAGGCTG GGCGAAGGGG AGCTGCTTCT GGAGGTGCAG GGGGTCCGGG 
     1261 TGTCCGGCTT GCCCCGCTAT GACGTGCTGG GGGTCATCGA CAGCTGCAAG GAGGCCGTCA 
     1321 CCTTCAAGGC CGTCAGACAA GGAGGAAGGC TGAACAAGGA CCTGCGACAT TTTCTCAATC 
     1381 AGCGATTCCA GAAGGGGTCT CCTGATCATG AGCTCCAGCA GACCATAAGG GATAACCTTT 
     1441 ACCGCCATGC TGTGCCTTGC ACAACCCGAT CTCCCAGAGA AGGAGAAGTG CCTGGCGTGG 
     1501 ACTATAACTT TCTGACTGTG AAGGAGTTCT TGGACCTCGA GCAGAGTGGG ACTCTTCTGG 
     1561 AAGTCGGCAC CTATGAAGGA AACTATTATG GGACACCCAA GCCTCCTAGC CAGCCAGTCA 
     1621 GTGGGAAAGT GATCACGACG GATGCCTTGC ACAGCCTTCA GTCTGGCTCT AAGCAGTCGA 
     1681 CCCCGAAGCG AACCAAGTCC TACAATGATA TGCAAAATGC TGGCATAGTC CACGCGGAGA 
     1741 ATGAGGAGGA GGATGACGTT CCTGAAATGA ACAGCAGCTT TACAGCCGAT TCTGGTGAAC 
     1801 AAGAGGAGCA CACTCTCCAA GAAACAGCAT TACCACCTGT GAATAGTAGC ATCATCGCTG 
     1861 CTCCCATCAC GGACCCTTCT CAGAAGTTCC CTCAATACCT ACCTCTTTCT GCAGAGGATA 
     1921 ATTTAGGTCC TCTACCTGAA AACTGGGAGA TGGCCTATAC TGAAAATGGA GAAGTCTATT 
     1981 TTATAGACCA TAACACGAAA ACAACATCTT GGTTAGACCC TCGGTGCCTA AACAAGCAGC 
     2041 AGAAGCCACT GGAAGAGTGT GAAGATGATG AAGGGGTACA CACCGAGGAG CTGGACAGTG 
     2101 AACTAGAACT GCCTGCTGGT TGGGAAAAGA TTGAAGACCC TGTCTATGGT ATCTACTATG 
     2161 TAGACCACAT CAACAGGAAG ACACAATATG AGAACCCGGT TCTAGAAGCC AAACGGAAGA 
     2221 AGCAGCTTGA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA ACAGCAGCAG CAGCAGCAGC 
     2281 AGCAGCAGCA GACAGAAGAA TGGACAGAAG ATCACTCAGC CCTTGTGCCT CCTGTTATTC 
     2341 CAAACCACCC TCCAAGCAAT CCAGAGCCAG CCAGAGAAGT TCCACTTCAG GGCAAACCCT 
     2401 TTTTTACACG AAACCCTTCT GAGTTGAAAG GCAAGTTCAT TCACACAAAG CTGCGGAAAA 
     2461 GCAGTCGTGG CTTTGGCTTC ACGGTGGTTG GAGGGGATGA ACCTGATGAG TTTCTCCAGA 
     2521 TCAAGAGCTT GGTCCTAGAT GGTCCTGCTG CATTGGATGG CAAGATGGAA ACAGGGGATG 
     2581 TGATTGTAAG TGTGAATGAC ACCTGTGTTT TGGGACACAC ACATGCTCAA GTTGTGAAGA 
     2641 TCTTCCAGTC CATCCCCATT GGTGCCAGCG TGGACCTTGA ACTCTGCCGA GGTTATCCAT 
     2701 TGCCTTTTGA TCCAGATGAC CCCAATACAA GTTTAGTGAC CTCGGTAGCC ATTTTGGATA 
     2761 AAGAACCAAT TATTGTGAAT GGGCAAGAGA CCTATGATTC ACCAGCTAGC CACAGTAGTA 
     2821 AAACAGGCAA AGTCAATGGC ATGAAGGATG CCAGGCCAAG CAGCCCAGCG GACGTGGCTT 
     2881 CAAATAGTTC TCATGGTTAT CCTAATGACA CTGTTTCTTT GGCTTCCTCC ATAGCCACTC 
     2941 AGCCAGAACT CATAACTGTT CATATTGTCA AAGGGCCAAT GGGCTTTGGT TTTACTATCG 
     3001 CAGACAGTCC TGGTGGGGGT GGCCAAAGAG TGAAACAGAT TGTTGACAGC CCAAGGTGCC 
     3061 GAGGCCTGAA AGAAGGGGAT CTCATAGTGG AAGTTAATAA GAAGAACGTG CAGGCCCTAA 
     3121 CTCACAACCA AGTGGTGGAT ATGCTGGTTG AGTGTCCTAA GGGAAGTGAG GTCACATTGT 
     3181 TGGTGCAACG AGGAGGGCTG CCAGTTCCCA AGAAGAGCCC AAAGTCGCAA CCACTGGAAA 
     3241 GGAAAGACAG CCAGAATAGT TCTCAGCACA GTGTTTCCAG CCACCGAAGC CTGCACACAG 
     3301 CATCCCCAAG CCACAGCACA CAGGTGCTCC CCGAGTTCCC ACCTGCAGAG GCCCAAGCTC 
     3361 CAGATCAAAC TGACAGCTCT GGCCAGAAAA AACCAGATCC TTTTAAAATC TGGGCCCAGT 
     3421 CCAGGAGCAT GTATGAAAAC CGACCTATGT CACCTTCACC TGCGTCGGGA TTGAGCAAGG 
     3481 GTGAGAGAGA GAGAGAAATC AATTCCACGA ATTTTGGAGA ATGTCCGATT CCAGATTACC 
     3541 AGGAACAGGA CATCTTCCTC TGGAGAAAAG AGACTGGATT TGGATTTAGG ATTCTGGGTG 
     3601 GAAATGAACC AGGGGAACCT ATTTATATTG GTCACATCGT ACCACTGGGT GCTGCTGATA 
     3661 CTGACGGCCG CCTGAGGTCT GGAGATGAAT TAATCTGTGT GGATGGGACG CCAGTAATTG 
     3721 GAAAATCACA CCAGCTTGTG GTCCAGCTTA TGCAACAAGC TGCCAAGCAA GGCCACGTCA 
     3781 ATCTCACGGT GCGGCGTAAA GTGGTTTTTG CGGTGCCCAA AACCGAGAAC GAGGTGCCCT 
     3841 CGCCAGCCTC CTCTCATCAC AGTAGCAACC AGCCGGCCTC GCTGACAGAA GAGAAGCGCA 
     3901 CTCCGCAGGG CAGCCAGAAC TCGCTGAACA CGGTGAGCTC GGGCAGCGGC AGCACCAGCG 
     3961 GCATCGGCAG TGGCGGCGGC GGGGGCAGCG GCGTGGTCAG CACCGTGGTG CAGCCCTACG 
     4021 ACGTGGAGAT CCGGCGCGGG GAGAACGAGG GCTTCGGCTT CGTCATCGTG TCCTCGGTGA 
     4081 GCAGGCCCGA AGCAGGCACG ACCTTTGCAG GCAATGCATG TGTGGCTATG CCTCACAAAA 
     4141 TAGGTCGGAT TATTGAGGGG AGCCCTGCTG ACCGCTGTGG CAAGCTGAAA GTAGGAGACC 
     4201 GGATCTTGGC AGTAAATGGA TGTTCCATCA CCAACAAATC CCATTCAGAC ATTGTGAACC 
     4261 TAATCAAGGA AGCGGGAAAC ACAGTTACCC TCCGCATCAT TCCTGGGGAT GAGTCCTCGA 
     4321 ATGCCACCTT GCTGACCAAT GCAGAGAAGA TTGCCACCAT CACCACCACA CACACCCCTT 
     4381 CTCAGCAAGG GACCCAGGAG ACAAGGAATA CCACCAAACC AAAGCAGGAA TCTCAATTTG 
     4441 AGTTCAAAGC ACCCCAAGCA ACACAGGAGC AAGATTTTTA CACTGTGGAA CTGGAAAGAG 
     4501 GAGCCAAGGG ATTTGGCTTT AGCCTTCGAG GAGGCCGAGA GTATAACATG GACCTCTATG 
     4561 TTCTGCGCTT AGCAGAGGAC GGTCCTGCGG AGAGGTGTGG AAAGATGAGG ATTGGTGATG 
     4621 AAATTTTAGA GATCAATGGT GAGACCACCA AAAACATGAA GCATTCTCGA GCTATAGAAC 
     4681 TGATTAAGAA TGGTGGCCGC AGAGTTCGTC TGTTTCTGAA GCGGGGAGAC GGCTCAGTAC 
     4741 CAGAATATGG TGGGTCAAAC TATGAAAACA TTCCTTCCTT CCCTGGCATG ACTCCAACGC 
     4801 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4861 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4921 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4981 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5041 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5101 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5161 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5221 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5281 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5341 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5401 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5461 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5521 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5581 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5641 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6061 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6121 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6181 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6241 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6301 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6361 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6421 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6481 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6541 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6601 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6661 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6721 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6781 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6841 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6901 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6961 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7021 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7081 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7141 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7201 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7261 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7321 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7381 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7441 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7501 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7561 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7621 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7681 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7741 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7801 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7861 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8161 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8641 GCGAATTTT
//