LOCUS       RC220213                6792 bp    DNA     circular  SYN 
DEFINITION  NRG1 (Myc-DDK-tagged)-Human neuregulin 1 (NRG1), transcript variant HRG-beta2
ACCESSION   NM_013957
VERSION     NM_013957.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2949..3040 
                     /label="Myc-DDK"  
     misc_feature    3070..3655 
                     /label="PolyA signal"  
     misc_feature    3127..3146 
                     /label="Restriction Enzyme"  
     misc_feature    3931..4603 
                     /label="ColE1"  
     misc_feature    5033..5827 
                     /label="Kan/Neo"  
     misc_feature    5862..6164 
                     /label="SV40 ori"  
     misc_feature    6250..6727 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2931..2937 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2931 
                     /label="NRG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCGAGCGC AAAGAAGGCA GAGGCAAAGG GAAGGGCAAG AAGAAGGAGC 
     1081 GAGGCTCCGG CAAGAAGCCG GAGTCCGCGG CGGGCAGCCA GAGCCCAGCC TTGCCTCCCC 
     1141 GATTGAAAGA GATGAAAAGC CAGGAATCGG CTGCAGGTTC CAAACTAGTC CTTCGGTGTG 
     1201 AAACCAGTTC TGAATACTCC TCTCTCAGAT TCAAGTGGTT CAAGAATGGG AATGAATTGA 
     1261 ATCGAAAAAA CAAACCACAA AATATCAAGA TACAAAAAAA GCCAGGGAAG TCAGAACTTC 
     1321 GCATTAACAA AGCATCACTG GCTGATTCTG GAGAGTATAT GTGCAAAGTG ATCAGCAAAT 
     1381 TAGGAAATGA CAGTGCCTCT GCCAATATCA CCATCGTGGA ATCAAACGAG ATCATCACTG 
     1441 GTATGCCAGC CTCAACTGAA GGAGCATATG TGTCTTCAGA GTCTCCCATT AGAATATCAG 
     1501 TATCCACAGA AGGAGCAAAT ACTTCTTCAT CTACATCTAC ATCCACCACT GGGACAAGCC 
     1561 ATCTTGTAAA ATGTGCGGAG AAGGAGAAAA CTTTCTGTGT GAATGGAGGG GAGTGCTTCA 
     1621 TGGTGAAAGA CCTTTCAAAC CCCTCGAGAT ACTTGTGCAA GTGCCCAAAT GAGTTTACTG 
     1681 GTGATCGCTG CCAAAACTAC GTAATGGCCA GCTTCTACAA GGCGGAGGAG CTGTACCAGA 
     1741 AGAGAGTGCT GACCATAACC GGCATCTGCA TCGCCCTCCT TGTGGTCGGC ATCATGTGTG 
     1801 TGGTGGCCTA CTGCAAAACC AAGAAACAGC GGAAAAAGCT GCATGACCGT CTTCGGCAGA 
     1861 GCCTTCGGTC TGAACGAAAC AATATGATGA ACATTGCCAA TGGGCCTCAC CATCCTAACC 
     1921 CACCCCCCGA GAATGTCCAG CTGGTGAATC AATACGTATC TAAAAACGTC ATCTCCAGTG 
     1981 AGCATATTGT TGAGAGAGAA GCAGAGACAT CCTTTTCCAC CAGTCACTAT ACTTCCACAG 
     2041 CCCATCACTC CACTACTGTC ACCCAGACTC CTAGCCACAG CTGGAGCAAC GGACACACTG 
     2101 AAAGCATCCT TTCCGAAAGC CACTCTGTAA TCGTGATGTC ATCCGTAGAA AACAGTAGGC 
     2161 ACAGCAGCCC AACTGGGGGC CCAAGAGGAC GTCTTAATGG CACAGGAGGC CCTCGTGAAT 
     2221 GTAACAGCTT CCTCAGGCAT GCCAGAGAAA CCCCTGATTC CTACCGAGAC TCTCCTCATA 
     2281 GTGAAAGGTA TGTGTCAGCC ATGACCACCC CGGCTCGTAT GTCACCTGTA GATTTCCACA 
     2341 CGCCAAGCTC CCCCAAATCG CCCCCTTCGG AAATGTCTCC ACCCGTGTCC AGCATGACGG 
     2401 TGTCCATGCC TTCCATGGCG GTCAGCCCCT TCATGGAAGA AGAGAGACCT CTACTTCTCG 
     2461 TGACACCACC AAGGCTGCGG GAGAAGAAGT TTGACCATCA CCCTCAGCAG TTCAGCTCCT 
     2521 TCCACCACAA CCCCGCGCAT GACAGTAACA GCCTCCCTGC TAGCCCCTTG AGGATAGTGG 
     2581 AGGATGAGGA GTATGAAACG ACCCAAGAGT ACGAGCCAGC CCAAGAGCCT GTTAAGAAAC 
     2641 TCGCCAATAG CCGGCGGGCC AAAAGAACCA AGCCCAATGG CCACATTGCT AACAGATTGG 
     2701 AAGTGGACAG CAACACAAGC TCCCAGAGCA GTAACTCAGA GAGTGAAACA GAAGATGAAA 
     2761 GAGTAGGTGA AGATACGCCT TTCCTGGGCA TACAGAACCC CCTGGCAGCC AGTCTTGAGG 
     2821 CAACACCTGC CTTCCGCCTG GCTGACAGCA GGACTAACCC AGCAGGCCGC TTCTCGACAC 
     2881 AGGAAGAAAT CCAGGCCAGG CTGTCTAGTG TAATTGCTAA CCAAGACCCT ATTGCTGTAA 
     2941 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3001 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3061 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3121 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3181 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3241 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3301 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3361 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3421 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3481 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3541 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     3601 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     3661 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     3721 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     3781 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     3841 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     3901 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     3961 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4021 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4081 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4141 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4201 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4261 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4321 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4381 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4441 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4501 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4561 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     4621 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     4681 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     4741 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     4801 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     4861 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     4921 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     4981 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5041 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5101 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5161 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5221 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5281 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5341 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5401 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5461 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5521 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     5581 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     5641 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     5701 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     5761 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     5821 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     5881 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     5941 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6001 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6061 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6121 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6181 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6241 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6301 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6361 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6421 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6481 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6541 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     6601 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     6661 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     6721 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     6781 AACGCGAATT TT
//