LOCUS       RC220179                9297 bp    DNA     circular  SYN 
DEFINITION  KIAA0586 (Myc-DDK-tagged)-Human KIAA0586 (KIAA0586)
ACCESSION   NM_014749
VERSION     NM_014749.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5454..5545 
                     /label="Myc-DDK"  
     misc_feature    5575..6160 
                     /label="PolyA signal"  
     misc_feature    5632..5651 
                     /label="Restriction Enzyme"  
     misc_feature    6436..7108 
                     /label="ColE1"  
     misc_feature    7538..8332 
                     /label="Kan/Neo"  
     misc_feature    8367..8669 
                     /label="SV40 ori"  
     misc_feature    8755..9232 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5436..5442 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5436 
                     /label="KIAA0586"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGGCTCT GAGGTCAGCT TGGAGAAGAA AAAAAAGATT AAGATGCCAG 
     1081 TGAAGAGACT TCGTGAGGTA GTTTCTCAAA ATCATGGAGA TCATTTGGTT TTGCTGAAAG 
     1141 ATGAGTTGCC CTGTGTTCCT CCGGCATTGT CTGCAAATAA ACGTCTTCCT GTTGGAACGG 
     1201 GGACTAGTTT GAATGGAACA TCACGTGGTT CATCAGACTT AACTTCTGCT AGAAATTGTT 
     1261 ACCAGCCTCT ATTAGAAAAT CCCATGGTGT CAGAAAGTGA TTTTTCTAAA GACGTTGCAG 
     1321 TGCAAGTGTT GCCTTTGGAT AAAATAGAAG AGAACAACAA GCAAAAAGCA AATGACATCT 
     1381 TCATTTCTCA GTATACAATG GGACAGAAAG ATGCTCTAAG AACAGTTTTA AAGCAAAAAG 
     1441 CTCAAAGCAT GCCTGTTTTT AAGGAAGTAA AGGTACATCT GTTAGAAGAT GCAGGCATAG 
     1501 AGAAGGATGC TGTTACTCAG GAGACTAGAA TTTCACCCAG TGGAATTGAT TCAGCTACAA 
     1561 CCGTGGCTGC AGCAACTGCT GCTGCCATTG CAACCGCAGC TCCGTTGATA AAGGTGCAGA 
     1621 GTGATTTGGA AGCAAAAGTC AATTCTGTTA CAGAATTACT TAGTAAATTA CAGGAGACTG 
     1681 ATAAACACCT GCAACGTGTT ACAGAGCAGC AAACAAGCAT TCAGAGGAAA CAAGAGAAAT 
     1741 TACATTGTCA TGATCACGAA AAGCAAATGA ATGTGTTTAT GGAGCAGCAC ATAAGGCATC 
     1801 TTGAAAAGTT ACAACAACAA CAAATAGATA TTCAGACTCA TTTTATTAGT GCTGCACTCA 
     1861 AGACTAGTAG TTTTCAGCCT GTTAGTATGC CCTCCTCCAG AGCAGTGGAA AAGTATTCCG 
     1921 TAAAACCAGA ACACCCTAAT CTTGGTAGCT GTAATCCATC TTTATATAAC ACATTTGCTT 
     1981 CCAAACAAGC ACCTTTAAAA GAAGTTGAAG ATACGAGTTT TGATAAACAG AAATCTCCTT 
     2041 TGGAGACACC AGCACCTCGC AGATTTGCTC CTGTACCTGT TTCAAGGGAT GATGAACTAT 
     2101 CAAAGAGGGA AAATCTTTTG GAAGAAAAAG AAAATATGGA AGTGTCGTGT CACAGAGGAA 
     2161 ATGTAAGACT ATTGGAACAA ATTTTGAATA ATAATGATTC TTTGACAAGA AAAAGTGAAT 
     2221 CATCAAACAC CACCTCACTA ACTAGGTCAA AAATAGGATG GACTCCTGAG AAAACAAACA 
     2281 GATTTCCTTC CTGTGAAGAG CTAGAAACAA CTAAAGTGAC TATGCAGAAG TCTGATGATG 
     2341 TTCTTCATGA CCTTGGCCAA AAAGAGAAAG AAACAAATAG CATGGTCCAG CCAAAAGAAT 
     2401 CTCTGAGTAT GTTGAAGCTT CCAGATCTTC CACAGAATTC TGTTAAGCTT CAAACAACCA 
     2461 ATACAACAAG ATCTGTATTG AAAGATGCTG AGAAGATTTT GAGAGGAGTA CAAAACAATA 
     2521 AAAAAGTACT TGAAGAAAAC CTGGAAGCTA TTATTCGTGC AAAAGATGGA GCTGCCATGT 
     2581 ATTCGCTTAT CAATGCTTTA TCTACCAACA GAGAGATGTC AGAGAAAATT AGGATCAGAA 
     2641 AGACAGTGGA TGAATGGATT AAAACTATTT CTGCAGAAAT TCAGGGGCTT TTGAAAGCAA 
     2701 CCACAGTAAT ACAAGATGAA GATTATATGT TACAAGTCTA TGGAAAGCCA GTTTATCAGG 
     2761 GCCATCGAAG CACTCTTAAA AAAGGACCAT ATCTCAGATT TAATTCTCCA TCTCCTAAGT 
     2821 CCAGACCACA GAGACCAAAA GTAATAGAAC GAGTTAAAGG CACTAAGGTA AAGTCAATAA 
     2881 GAACACAGAC TGACTTCTAT GCAACAAAAC CTAAGAAGAT GGATTCTAAA ATGAAACATT 
     2941 CTGTTCCTGT GTTACCTCAT GGCGATCAGC AATATTTGTT CAGCCCAAGT AGAGAAATGC 
     3001 CTACTTTTTC AGGTACATTG GAAGGTCATC TGATTCCTAT GGCAATTCTT TTAGGACAAA 
     3061 CCCAAAGTAA TAGTGATACC ATGCCACCTG CTGGAGTGAT TGTCAGCAAG CCACACCCTG 
     3121 TAACTGTGAC TACTTCTATT CCTCCATCAT CTCGAAAAGT AGAAACTGGA GTAAAGAAAC 
     3181 CTAACATAGC CATTGTAGAA ATGAAGTCAG AAAAAAAGGA TCCTCCTCAG CTTACTGTGC 
     3241 AGGTATTACC CAGTGTAGAT ATTGACAGCA TTTCAAATAG TAGTGCTGAT GTCCTTTCAC 
     3301 CTCTGTCTAG CCCCAAAGAA GCATCTCTTC CTCCTGTGCA AACTTGGATA AAGACTCCAG 
     3361 AAATTATGAA GGTAGATGAA GAAGAGGTGA AGTTTCCAGG AACTAACTTT GATGAAATAA 
     3421 TCGATGTCAT ACAGGAAGAA GAAAAATGTG ATGAAATTCC AGACTCTGAA CCAATTCTGG 
     3481 AGTTTAACAG AAGTGTTAAA GCTGATTCTA CAAAATATAA TGGTCCTCCA TTTCCGCCAG 
     3541 TTGCTTCTAC TTTTCAGCCC ACTGCTGATA TTCTGGATAA AGTAATTGAG AGAAAAGAAA 
     3601 CACTGGAAAA TAGCTTAATT CAATGGGTAG AGCAAGAAAT AATGTCAAGA ATTATCTCTG 
     3661 GGCTCTTTCC AGTCCAGCAA CAGATTGCAC CTAGTATCAG TGTTTCAGTC AGTGAGACAA 
     3721 GTGAACCACT GACTTCTGAC ATTGTGGAAG GAACAAGCAG TGGCGCCCTC CAGCTTTTTG 
     3781 TTGATGCTGG TGTTCCTGTG AACTCAAATG TGATTAAACA TTTTGTTAAC GAAGCTCTTG 
     3841 CTGAGACCAT TGCTGTCATG CTGGGTGACA GAGAAGCAAA GAAGCAAGGT CCTGTTGCTA 
     3901 CAGGTGTTTC TGGGGATGCT TCAACAAATG AAACATATTT GCCGGCAAGA GTGTGCACCC 
     3961 CACTGCCTAC CCCACAGCCT ACGCCTCCTT GCTCACCTTC ATCACCTGCT AAGGAGTGTG 
     4021 TTTTGGTAAA GACTCCAGAT TCTTCTCCCT GTGATTCGGA TCATGATATG GCTTTTCCTG 
     4081 TGAAAGAAAT ATGTGCTGAA AAAGGAGATG ATATGCCTGC CATCATGCTT GTTAATACTC 
     4141 CAACAGTTAC CCCTACTACT ACACCTCCTC CAGCGGCGGC AGTTTTTACC CCAACTTTGT 
     4201 CAGATATTTC CATTGATAAA TTGAAGGTAT CAAGCCCAGA GCTTCCCAAG CCATGGGGTG 
     4261 ATGGAGACCT GCCACTGGAA GAAGAGAACC CTAACTCACC TCAAGAAGAA CTTCATCCAA 
     4321 GAGCTATTGT AATGTCTGTG GCTAAGGATG AAGAACCAGA GAGTATGGAT TTCCCTGCTC 
     4381 AGCCTCCACC TCCAGAGCCA GTTCCCTTTA TGCCATTTCC TGCCGGCACC AAGGCCCCTT 
     4441 CCCCCTCACA GATGCCAGGT TCTGATTCAT CAACACTGGA GAGCACATTG AGTGTTACTG 
     4501 TCACTGAAAC TGAAACTTTA GATAAACCCA TCTCTGAAGG AGAGATTTTA TTTAGCTGTG 
     4561 GTCAAAAATT GGCCCCCAAG ATTTTAGAAG ATATAGGACT GTACCTGACA AACCTTAATG 
     4621 ATAGCTTATC CAGCACTCTG CATGATGCCG TTGAAATGGA GGATGATCCT CCTAGTGAAG 
     4681 GGCAAGTGAT TAGGATGTCC CATAAAAAAT TTCATGCAGA TGCAATTCTT TCTTTTGCTA 
     4741 AACAAAACCA GGAGTCAGCA GTTTCCCAGC AAGCAGTCTA TCATTCAGAG GACTTGGAAA 
     4801 ACAGTGTGGG TGAACTTAGT GAAGGACAAA GACCCCAGCT AACAGCGGCA GCAGAGAACA 
     4861 TCTTAATGGG ACATTCTCTC TATATGCAGC CACCTGTCAC TAATACACAG TCTTTGGATC 
     4921 AACAATGTGA TCCTAAACCA TTATCTCGGC AATTTGACAC AGTTTCAGGT AGTATTTATG 
     4981 AAGATTCATG TGCTAGTCAT GGTCCAATGA GTTTGGGAGA ATTGGAGTTG GAGCCAAATT 
     5041 CTAAGCTGGT TCTTCCCACA ACACTTCTGA CAGCACAAGA AAATGATGTT AATTTACCAG 
     5101 TAGCCGCTGA AGATTTTTCC CAGTACCAAC TAAAGCAAAA TCAGGATGTT AAGCAAGTTG 
     5161 AACACAAACC ATCACAAAGT TACCTACGTG TTAGAAATAA ATCTGATATT GCACCTTCAC 
     5221 AGCAACAAGT TTCACCAGGT GATATGGATC GGACACAAAT TGAGCTTAAT CCGTACCTCA 
     5281 CATGTGTATT TTCAGGTGGG AAAGCAGTGC CACTCTCCGC TTCACAGATG CCCCCTGCCA 
     5341 AGATGTCAGT GATGCTGCCG TCAGTGAACC TCGAGGACTG CTCTCAGTCT CTGAGTCTCA 
     5401 GCACAATGCA GGAGGACATG GAGTCTTCGG GGGCAGATAC CTTCACGCGT ACGCGGCCGC 
     5461 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5521 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5581 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5641 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5701 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5761 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5821 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5881 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5941 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6001 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6061 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6121 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6181 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6241 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6301 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6361 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6421 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6481 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6541 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6601 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6661 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6721 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6781 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6841 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6901 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6961 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7021 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7081 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7141 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7201 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7261 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7321 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7381 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7441 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7501 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7561 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7621 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7681 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7741 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7801 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7861 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7921 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7981 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8041 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8101 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8161 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8221 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8281 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8341 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8401 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8461 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8521 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8581 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8641 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8701 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8761 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8821 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8881 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8941 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9001 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9061 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9121 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9181 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9241 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//