LOCUS       RC220164                7017 bp    DNA     circular  SYN 
DEFINITION  TCF12 (Myc-DDK-tagged)-Human transcription factor 12 (TCF12), transcript variant 1
ACCESSION   NM_207036
VERSION     NM_207036.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3172..3263 
                     /label="Myc-DDK"  
     misc_feature    3293..3878 
                     /label="PolyA signal"  
     misc_feature    3350..3369 
                     /label="Restriction Enzyme"  
     misc_feature    4154..4826 
                     /label="ColE1"  
     misc_feature    5256..6050 
                     /label="Kan/Neo"  
     misc_feature    6085..6387 
                     /label="SV40 ori"  
     misc_feature    6473..6950 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3147..3154 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3147 
                     /label="TCF12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCCCCAG CAACAACGCA TGGCCGCTAT AGGGACCGAC AAGGAGCTGA 
     1081 GCGACCTACT GGACTTCAGT GCGATGTTTT CCCCACCTGT TAATAGTGGG AAAACTAGAC 
     1141 CAACTACACT GGGAAGCAGT CAATTCAGTG GATCAGGTAT TGATGAAAGA GGAGGTACAA 
     1201 CATCTTGGGG AACAAGTGGT CAACCAAGTC CTTCCTATGA TTCATCTAGA GGTTTTACAG 
     1261 ACAGCCCTCA TTACAGTGAT CACTTGAATG ACAGTCGATT AGGAGCCCAT GAAGGCTTGT 
     1321 CCCCAACACC TTTCATGAAC TCAAATCTGA TGGGAAAAAC ATCAGAGAGA GGCTCATTTT 
     1381 CCCTGTACAG CAGAGATACT GGATTACCAG GCTGTCAATC TAGTCTCCTG AGACAAGATC 
     1441 TGGGGCTTGG GAGCCCAGCA CAGCTATCTT CTTCAGGAAA ACCTGGGACA GCATACTATT 
     1501 CATTCTCTGC TACAAGTTCC AGGAGGAGAC CACTCCATGA CTCTGCAGCG CTTGATCCCT 
     1561 TGCAAGCAAA AAAAGTCAGA AAGGTGCCTC CTGGTTTGCC TTCTTCTGTA TATGCACCAT 
     1621 CCCCAAATTC AGATGATTTC AACCGTGAAT CTCCTAGTTA TCCATCTCCT AAGCCACCAA 
     1681 CCAGTATGTT CGCTAGCACT TTCTTTATGC AAGATGGGAC CCACAATTCT TCTGACCTTT 
     1741 GGAGTTCATC AAATGGGATG AGCCAGCCTG GTTTTGGTGG AATTCTGGGG ACCTCCACTT 
     1801 CCCACATGTC TCAATCCAGT AGTTATGGCA ACCTTCATTC ACATGACCGC TTGAGTTATC 
     1861 CTCCACACTC AGTTTCACCA ACAGACATAA ACACGAGTCT TCCACCAATG TCCAGCTTTC 
     1921 ATCGCGGCAG TACCAGCAGT TCACCTTACG TTGCTGCCTC ACACACTCCT CCCATCAATG 
     1981 GATCAGACAG CATTCTAGGA ACCAGAGGGA ATGCTGCTGG AAGCTCACAG ACAGGTGATG 
     2041 CACTTGGAAA GGCTTTGGCA TCTATTTATT CTCCTGACCA TACCAGCAGT AGTTTTCCGT 
     2101 CAAATCCATC AACACCAGTT GGATCACCTT CACCTCTCAC AGGTACCAGT CAGTGGCCAA 
     2161 GACCTGGAGG GCAAGCACCT TCATCCCCAA GCTATGAAAA CTCACTCCAC TCCCTGAAAA 
     2221 ATCGAGTTGA GCAGCAACTT CACGAGCATT TGCAAGATGC AATGTCCTTC TTAAAGGATG 
     2281 TCTGTGAGCA GTCTCGAATG GAGGATCGTT TAGACAGACT GGATGATGCA ATCCATGTGC 
     2341 TGCGGAACCA TGCTGTGGGA CCTTCCACCA GTTTGCCTGC TGGTCACAGT GATATACATA 
     2401 GTTTATTGGG ACCATCCCAT AATGCACCAA TTGGAAGCCT CAATTCAAAC TATGGAGGAT 
     2461 CAAGCCTTGT TGCAAGCAGT CGATCAGCTT CAATGGTTGG AACTCATCGG GAAGACTCTG 
     2521 TCAGTCTCAA TGGCAATCAT TCAGTCCTGT CTAGTACAGT CACTACTTCA AGCACAGACC 
     2581 TGAACCATAA AACACAAGAA AATTATAGAG GTGGCTTGCA AAGTCAGTCT GGAACTGTTG 
     2641 TTACAACAGA AATCAAGACT GAAAACAAAG AAAAGGATGA AAACCTTCAT GAACCTCCTT 
     2701 CATCAGATGA CATGAAGTCA GATGATGAAT CCTCCCAAAA AGATATCAAG GTTTCATCTA 
     2761 GAGGCAGAAC AAGCAGTACT AATGAAGATG AGGATTTGAA CCCTGAACAG AAGATAGAAA 
     2821 GGGAGAAGGA GAGGCGGATG GCTAACAATG CCAGAGAACG CTTACGCGTG CGGGATATTA 
     2881 ATGAAGCATT CAAAGAGCTT GGCCGAATGT GTCAGCTTCA CTTGAAGAGT GAAAAACCCC 
     2941 AAACAAAACT CCTTATTCTT CATCAAGCCG TGGCAGTCAT CCTTAGTCTA GAACAGCAAG 
     3001 TCAGAGAGAG GAACCTTAAC CCCAAAGCAG CCTGCCTTAA GAGAAGGGAA GAAGAAAAAG 
     3061 TTTCTGCCGT ATCGGCAGAG CCGCCAACCA CACTGCCAGG AACCCATCCT GGGCTTAGTG 
     3121 AAACTACCAA CCCTATGGGT CATATGAGCG GACCGAGCGG ACCGACGCGT ACGCGGCCGC 
     3181 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3241 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3301 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3361 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3421 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3481 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3541 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3601 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3661 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3721 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3781 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3841 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3901 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     3961 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4021 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4081 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4141 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4201 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4261 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4321 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4381 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4441 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4501 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4561 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4621 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4681 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4741 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4801 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4861 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4921 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     4981 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5041 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5101 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5161 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5221 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5281 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5341 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5401 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5461 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5521 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5581 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5641 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5701 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5761 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5821 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5881 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     5941 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6001 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6061 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6121 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6181 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6241 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6301 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6361 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6421 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6481 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6541 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6601 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6661 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6721 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6781 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6841 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6901 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     6961 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//