LOCUS RC220160 7494 bp DNA circular SYN DEFINITION TRPV4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1 ACCESSION NM_021625 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3651..3742 /label="Myc-DDK" misc_feature 3772..4357 /label="PolyA signal" misc_feature 3829..3848 /label="Restriction Enzyme" misc_feature 4633..5305 /label="ColE1" misc_feature 5735..6529 /label="Kan/Neo" misc_feature 6564..6866 /label="SV40 ori" misc_feature 6952..7429 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3633..3639 /label="Restriction Enzyme" misc_feature 1020..3633 /label="TRPV4" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCGGATTCC AGCGAAGGCC CCCGCGCGGG GCCCGGGGAG GTGGCTGAGC 1081 TCCCCGGGGA TGAGAGTGGC ACCCCAGGTG GGGAGGCTTT TCCTCTCTCC TCCCTGGCCA 1141 ATCTGTTTGA GGGGGAGGAT GGCTCCCTTT CGCCCTCACC GGCTGATGCC AGTCGCCCTG 1201 CTGGCCCAGG CGATGGGCGA CCAAATCTGC GCATGAAGTT CCAGGGCGCC TTCCGCAAGG 1261 GGGTGCCCAA CCCCATCGAT CTGCTGGAGT CCACCCTATA TGAGTCCTCG GTGGTGCCTG 1321 GGCCCAAGAA AGCACCCATG GACTCACTGT TTGACTACGG CACCTATCGT CACCACTCCA 1381 GTGACAACAA GAGGTGGAGG AAGAAGATCA TAGAGAAGCA GCCGCAGAGC CCCAAAGCCC 1441 CTGCCCCTCA GCCGCCCCCC ATCCTCAAAG TCTTCAACCG GCCTATCCTC TTTGACATCG 1501 TGTCCCGGGG CTCCACTGCT GACCTGGACG GGCTGCTCCC ATTCTTGCTG ACCCACAAGA 1561 AACGCCTAAC TGATGAGGAG TTTCGAGAGC CATCTACGGG GAAGACCTGC CTGCCCAAGG 1621 CCTTGCTGAA CCTGAGCAAT GGCCGCAACG ACACCATCCC TGTGCTGCTG GACATCGCGG 1681 AGCGCACCGG CAACATGCGG GAGTTCATTA ACTCGCCCTT CCGTGACATC TACTATCGAG 1741 GTCAGACAGC CCTGCACATC GCCATTGAGC GTCGCTGCAA ACACTACGTG GAACTTCTCG 1801 TGGCCCAGGG AGCTGATGTC CACGCCCAGG CCCGTGGGCG CTTCTTCCAG CCCAAGGATG 1861 AGGGGGGCTA CTTCTACTTT GGGGAGCTGC CCCTGTCGCT GGCTGCCTGC ACCAACCAGC 1921 CCCACATTGT CAACTACCTG ACGGAGAACC CCCACAAGAA GGCGGACATG CGGCGCCAGG 1981 ACTCGCGAGG CAACACAGTG CTGCATGCGC TGGTGGCCAT TGCTGACAAC ACCCGTGAGA 2041 ACACCAAGTT TGTTACCAAG ATGTACGACC TGCTGCTGCT CAAGTGTGCC CGCCTCTTCC 2101 CCGACAGCAA CCTGGAGGCC GTGCTCAACA ACGACGGCCT CTCGCCCCTC ATGATGGCTG 2161 CCAAGACGGG CAAGATTGGG ATCTTTCAGC ACATCATCCG GCGGGAGGTG ACGGATGAGG 2221 ACACACGGCA CCTGTCCCGC AAGTTCAAGG ACTGGGCCTA TGGGCCAGTG TATTCCTCGC 2281 TTTATGACCT CTCCTCCCTG GACACGTGTG GGGAAGAGGC CTCCGTGCTG GAGATCCTGG 2341 TGTACAACAG CAAGATTGAG AACCGCCACG AGATGCTGGC TGTGGAGCCC ATCAATGAAC 2401 TGCTGCGGGA CAAGTGGCGC AAGTTCGGGG CCGTCTCCTT CTACATCAAC GTGGTCTCCT 2461 ACCTGTGTGC CATGGTCATC TTCACTCTCA CCGCCTACTA CCAGCCGCTG GAGGGCACAC 2521 CGCCGTACCC TTACCGCACC ACGGTGGACT ACCTGCGGCT GGCTGGCGAG GTCATTACGC 2581 TCTTCACTGG GGTCCTGTTC TTCTTCACCA ACATCAAAGA CTTGTTCATG AAGAAATGCC 2641 CTGGAGTGAA TTCTCTCTTC ATTGATGGCT CCTTCCAGCT GCTCTACTTC ATCTACTCTG 2701 TCCTGGTGAT CGTCTCAGCA GCCCTCTACC TGGCAGGGAT CGAGGCCTAC CTGGCCGTGA 2761 TGGTCTTTGC CCTGGTCCTG GGCTGGATGA ATGCCCTTTA CTTCACCCGT GGGCTGAAGC 2821 TGACGGGGAC CTATAGCATC ATGATCCAGA AGATTCTCTT CAAGGACCTT TTCCGATTCC 2881 TGCTCGTCTA CTTGCTCTTC ATGATCGGCT ACGCTTCAGC CCTGGTCTCC CTCCTGAACC 2941 CGTGTGCCAA CATGAAGGTG TGCAATGAGG ACCAGACCAA CTGCACAGTG CCCACTTACC 3001 CCTCGTGCCG TGACAGCGAG ACCTTCAGCA CCTTCCTCCT GGACCTGTTT AAGCTGACCA 3061 TCGGCATGGG CGACCTGGAG ATGCTGAGCA GCACCAAGTA CCCCGTGGTC TTCATCATCC 3121 TGCTGGTGAC CTACATCATC CTCACCTTTG TGCTGCTCCT CAACATGCTC ATTGCCCTCA 3181 TGGGCGAGAC AGTGGGCCAG GTCTCCAAGG AGAGCAAGCA CATCTGGAAG CTGCAGTGGG 3241 CCACCACCAT CCTGGACATT GAGCGCTCCT TCCCCGTATT CCTGAGGAAG GCCTTCCGCT 3301 CTGGGGAGAT GGTCACCGTG GGCAAGAGCT CGGACGGCAC TCCTGACCGC AGGTGGTGCT 3361 TCAGGGTGGA TGAGGTGAAC TGGTCTCACT GGAACCAGAA CTTGGGCATC ATCAACGAGG 3421 ACCCGGGCAA GAATGAGACC TACCAGTATT ATGGCTTCTC GCATACCGTG GGCCGCCTCC 3481 GCAGGGATCG CTGGTCCTCG GTGGTACCCC GCGTGGTGGA ACTGAACAAG AACTCGAACC 3541 CGGACGAGGT GGTGGTGCCT CTGGACAGCA TGGGGAACCC CCGCTGCGAT GGCCACCAGC 3601 AGGGTTACCC CCGCAAGTGG AGGACTGATG ACGCCCCGCT CACGCGTACG CGGCCGCTCG 3661 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 3721 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 3781 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 3841 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 3901 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 3961 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 4021 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 4081 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 4141 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 4201 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 4261 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 4321 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 4381 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 4441 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 4501 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 4561 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 4621 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 4681 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 4741 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 4801 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 4861 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 4921 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 4981 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 5041 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 5101 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 5161 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 5221 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 5281 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 5341 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 5401 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 5461 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 5521 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 5581 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 5641 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 5701 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 5761 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 5821 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 5881 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 5941 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 6001 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 6061 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 6121 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 6181 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 6241 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 6301 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 6361 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 6421 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 6481 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 6541 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 6601 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 6661 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 6721 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 6781 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 6841 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 6901 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 6961 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 7021 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 7081 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 7141 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 7201 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 7261 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 7321 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 7381 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 7441 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT //