LOCUS       RC220121                6624 bp    DNA     circular  SYN 
DEFINITION  MATN4 (Myc-DDK-tagged)-Human matrilin 4 (MATN4), transcript variant 1
ACCESSION   NM_003833
VERSION     NM_003833.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2781..2872 
                     /label="Myc-DDK"  
     misc_feature    2902..3487 
                     /label="PolyA signal"  
     misc_feature    2959..2978 
                     /label="Restriction Enzyme"  
     misc_feature    3763..4435 
                     /label="ColE1"  
     misc_feature    4865..5659 
                     /label="Kan/Neo"  
     misc_feature    5694..5996 
                     /label="SV40 ori"  
     misc_feature    6082..6559 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2763..2769 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2763 
                     /label="MATN4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAGGCCTT CTTTGCTGGC CCGTGTTGCT GCTCCTTCTT CAGCCCTGGG 
     1081 AAACCCAGCT CCAGTTGACA GGTCCCAGGT GTCACACTGG GCCCCTGGAT CTGGTGTTCG 
     1141 TGATTGACAG CTCCCGCAGC GTGCGCCCTT TCGAGTTCGA GACCATGCGG CAGTTCCTCA 
     1201 TGGGCCTCCT CCGAGGCCTG AACGTGGGTC CCAACGCCAC GCGCGTTGGC GTGATCCAGT 
     1261 ATTCGAGTCA AGTGCAGAGC GTCTTCCCTC TCCGCGCGTT CTCTCGCCGC GAGGACATGG 
     1321 AGCGCGCCAT CCGCGACCTG GTGCCTCTGG CGCAAGGCAC CATGACGGGA CTGGCGATCC 
     1381 AGTACGCCAT GAACGTGGCC TTCAGTGTGG CCGAGGGCGC GCGACCGCCA GAGGAGCGCG 
     1441 TGCCGCGTGT CGCTGTCATC GTGACAGACG GGCGGCCCCA GGACCGCGTG GCCGAGGTGG 
     1501 CGGCACAGGC GCGCGCCAGC GGCATTGAAA TTTACGCGGT GGGGGTGCAG CGCGCGGACG 
     1561 TGGGCTCCCT GCGCGCCATG GCATCGCCCC CGCTAGACGA GCACGTCTTC CTCGTAGAGT 
     1621 CCTTCGACCT CATCCAGGAG TTCGGCCTGC AGTTCCAGAG CCGGCTGTGT GCCATTGATC 
     1681 TGTGTGCTGA AGGGACCCAT GGATGTGAGC ACCACTGCGT CAATTCCCCA GGCTCCTATT 
     1741 TCTGTCACTG CCAAGTTGGC TTTGTACTCC AGCAGGACCA GAGGAGCTGC AGGGCCATTG 
     1801 ACTACTGCAG CTTTGGGAAC CATAGCTGTC AGCATGAGTG TGTTAGCACC CCTGGTGGGC 
     1861 CACGGTGCCA CTGCAGAGAG GGCCATGACT TGCAGCCTGA TGGGAGGAGC TGTCAGGTCC 
     1921 GGGACCTTTG CAATGGCGTG GACCATGGCT GTGAGTTCCA GTGTGTGAGC GAGGGCCTCT 
     1981 CCTACCGCTG CCTGTGCCCC GAGGGGCGGC AACTTCAGGC AGATGGCAAG AGCTGCAACC 
     2041 GGTGCCGGGA AGGCCACGTG GACCTTGTTC TGCTGGTTGA TGGCTCCAAG AGCGTGCGTC 
     2101 CACAAAACTT CGAGCTAGTG AAGCGCTTCG TGAACCAGAT TGTGGACTTC CTAGATGTGT 
     2161 CCCCCGAGGG CACGCGGGTG GGGCTGGTGC AGTTCTCGAG CCGCGTGCGC ACCGAGTTCC 
     2221 CTCTGGGTCG CTACGGCACC GCAGCCGAGG TGAAGCAGGC GGTCCTGGCC GTGGAGTACA 
     2281 TGGAACGCGG CACCATGACA GGGCTGGCGT TGCGGCACAT GGTGGAGCAC AGCTTCTCCG 
     2341 AGGCGCAGGG TGCACGGCCC CGTGCCCTTA ACGTGCCTCG TGTTGGCCTG GTCTTCACGG 
     2401 ATGGCCGCTC CCAGGATGAC ATCTCGGTGT GGGCAGCGCG CGCCAAGGAG GAAGGCATCG 
     2461 TCATGTACGC CGTGGGCGTG GGCAAGGCGG TGGAGGCGGA GCTGCGCGAG ATCGCCTCGG 
     2521 AGCCAGCGGA ACTGCACGTG TCCTATGCCC CGGACTTCGG CACCATGACG CACCTGCTGG 
     2581 AGAACCTCAG AGGCAGCATC TGTCCAGAGG AGGGCATCAG CGCAGGGACA GAGCTTCGGA 
     2641 GCCCATGCGA ATGCGAAAGC CTCGTGGAGT TCCAGGGCCA CACGCTGGGG GCGCTCGAGA 
     2701 GCCTGACGCT GAACCTGGCC CAGCTGACGG CGCGCCTGGA GGATCTGGAG AACCAGCTGG 
     2761 CCAACCAGAA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     2821 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     2881 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     2941 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3001 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3061 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3121 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3181 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3241 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3301 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3361 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3421 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3481 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3541 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3601 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     3661 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     3721 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     3781 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     3841 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     3901 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     3961 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4021 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4081 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4141 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4201 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4261 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4321 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4381 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4441 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4501 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4561 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4621 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     4681 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     4741 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     4801 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     4861 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     4921 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     4981 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5041 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5101 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5161 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5221 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5281 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5341 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5401 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5461 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5521 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5581 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     5641 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     5701 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     5761 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     5821 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     5881 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     5941 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6001 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6061 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6121 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6181 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6241 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6301 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6361 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6421 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6481 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6541 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6601 TAACAAAAAT TTAACGCGAA TTTT
//