LOCUS       RC220120                8985 bp    DNA     circular  SYN 
DEFINITION  KIF24 (Myc-DDK-tagged)-Human kinesin family member 24 (KIF24)
ACCESSION   NM_194313
VERSION     NM_194313.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5142..5233 
                     /label="Myc-DDK"  
     misc_feature    5263..5848 
                     /label="PolyA signal"  
     misc_feature    5320..5339 
                     /label="Restriction Enzyme"  
     misc_feature    6124..6796 
                     /label="ColE1"  
     misc_feature    7226..8020 
                     /label="Kan/Neo"  
     misc_feature    8055..8357 
                     /label="SV40 ori"  
     misc_feature    8443..8920 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5124..5130 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5124 
                     /label="KIF24"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATCCTGG TTATATGAAT GTCTTTGTGA AGCTGAACTT GCACAGTATT 
     1081 ATTCTCATTT CACTGCCCTT GGCCTTCAGA AAATAGATGA ATTAGCCAAG ATTACAATGA 
     1141 AGGACTACTC CAAATTAGGA GTCCATGACA TGAACGACCG CAAACGTCTC TTCCAACTTA 
     1201 TCAAAATTAT TAAGATTATG CAAGAAGAAG ATAAAGCAGT CAGTATCCCA GAGCGTCATC 
     1261 TTCAGACAAG CAGCCTGCGC ATCAAATCTC AGGAATTAAG ATCTGGCCCT CGCAGACAGC 
     1321 TGAATTTTGA TTCTCCTGCT GACAATAAAG ACAGAAATGC CAGCAATGAT GGGTTTGAAA 
     1381 TGTGCAGTTT ATCAGATTTC TCTGCAAATG AACAGAAGTC CACTTACCTA AAAGTGCTAG 
     1441 AACACATGCT ACCAGATGAT TCCCAGTACC ATACAAAAAC AGGAATTCTG AATGCCACAG 
     1501 CTGGTGATTC CTATGTGCAA ACAGAAATCA GCACTTCACT CTTTTCACCA AATTACCTTT 
     1561 CTGCAATACT GGGGGATTGT GATATTCCCA TTATTCAAAG AATCTCTCAT GTTTCAGGGT 
     1621 ATAACTATGG AATCCCTCAT TCTTGTATCA GACAGAACAC TTCAGAGAAA CAGAATCCTT 
     1681 GGACTGAGAT GGAGAAAATC AGAGTTTGTG TTCGAAAACG CCCCCTGGGC ATGAGGGAGG 
     1741 TACGTCGTGG AGAAATTAAT ATTATTACTG TAGAAGACAA AGAAACTCTA CTTGTGCATG 
     1801 AGAAGAAAGA AGCAGTTGAC CTCACTCAAT ATATTCTGCA GCATGTTTTT TATTTTGATG 
     1861 AAGTCTTTGG TGAGGCGTGC ACCAATCAGG ATGTATACAT GAAGACTACT CACCCACTTA 
     1921 TTCAGCATAT TTTCAATGGA GGCAATGCCA CTTGCTTTGC TTATGGACAG ACAGGTGCTG 
     1981 GAAAGACCTA CACCATGATA GGAACTCATG AGAACCCAGG ATTGTATGCT CTAGCTGCCA 
     2041 AAGATATCTT CAGGCAACTA GAAGTGTCCC AGCCAAGAAA GCACCTCTTT GTGTGGATCA 
     2101 GCTTCTATGA AATTTACTGT GGACAGCTTT ATGACCTCCT AAATAGAAGA AAAAGGCTCT 
     2161 TTGCAAGAGA AGATAGCAAG CACATGGTGC AGATAGTGGG ACTGCAAGAG CTTCAGGTGG 
     2221 ACAGTGTGGA GCTCCTCTTA GAGGTGATCT TAAAGGGCAG CAAGGAGCGC AGCACTGGGG 
     2281 CCACTGGAGT TAATGCAGAC TCCTCCCGCT CCCATGCCGT CATCCAAATT CAGATCAAAG 
     2341 ATTCAGCCAA GAGGACATTT GGCAGGATCT CTTTTATTGA CTTGGCTGGC AGTGAAAGAG 
     2401 CAGCAGATGC AAGGGACTCA GATAGACAGA CAAAGATGGA AGGTGCAGAA ATAAATCAGA 
     2461 GTCTACTGGC TCTGAAGGAA TGTATCCGAG CACTGGATCA GGAACACACC CATACTCCCT 
     2521 TCAGGCAAAG CAAACTAACT CAGGTCCTGA AGGACTCTTT CATCGGCAAT GCCAAAACCT 
     2581 GCATGATCGC CAACATCTCA CCAAGCCACG TGGCCACTGA ACACACTCTC AACACCTTGC 
     2641 GCTATGCTGA CCGGGTCAAA GAACTAAAGA AAGGCATTAA GTGTTGCACT TCAGTTACCA 
     2701 GTCGAAATCG GACATCTGGA AACTCCTCTC CAAAACGAAT TCAGAGCTCC CCTGGGGCTT 
     2761 TGTCAGAGGA CAAATGTTCT CCCAAAAAAG TCAAGCTGGG ATTTCAGCAG TCACTCACAG 
     2821 TGGCAGCCCC TGGTTCCACG AGAGGGAAGG TCCATCCTCT GACCAGCCAC CCACCCAACA 
     2881 TTCCTTTTAC TTCTGCACCT AAGGTCTCTG GTAAAAGGGG TGGCTCCAGA GGGAGTCCTT 
     2941 CACAAGAGTG GGTCATTCAT GCTAGCCCTG TGAAAGGAAC TGTGCGCTCT GGACATGTGG 
     3001 CCAAAAAAAA GCCAGAAGAG TCAGCACCAT TGTGCTCTGA GAAAAATCGA ATGGGCAACA 
     3061 AAACTGTCCT TGGGTGGGAA AGCAGGGCCT CAGGCCCAGG AGAAGGCCTA GTGCGTGGTA 
     3121 AGCTGTCCAC CAAGTGCAAG AAAGTGCAGA CAGTGCAGCC AGTACAGAAG CAGCTTGTGT 
     3181 CTCGAGTTGA GCTCTCCTTT GGCAACGCCC ACCACAGGGC TGAGTACAGT CAAGACAGCC 
     3241 AGAGGGGCAC GCCTGCTAGG CCTGCCTCTG AAGCTTGGAC AAACATCCCG CCACATCAGA 
     3301 AGGAGAGGGA GGAACATCTG CGTTTCTATC ACCAGCAGTT CCAACAGCCA CCTCTCCTCC 
     3361 AACAGAAGTT AAAATACCAA CCACTGAAAA GGTCTTTACG CCAGTACAGG CCCCCAGAGG 
     3421 GTCAGCTCAC GAATGAGACT CCGCCTCTGT TCCACTCTTA CTCTGAAAAC CATGATGGAG 
     3481 CCCAAGTAGA GGAACTTGAT GACAGTGATT TCAGTGAAGA TTCTTTTTCA CACATCTCTA 
     3541 GTCAGAGGGC CACAAAGCAA AGGAACACCC TGGAGAATAG CGAAGACTCA TTCTTCCTGC 
     3601 ACCAGACGTG GGGACAGGGT CCTGAGAAGC AGGTGGCAGA AAGACAGCAG AGTCTGTTTT 
     3661 CTAGCCCCAG GACAGGTGAC AAGAAAGATC TAACTAAAAG CTGGGTGGAC TCCAGGGACC 
     3721 CCATAAACCA CAGAAGAGCA GCACTCGATC ACAGCTGCAG CCCAAGTAAG GGGCCCGTGG 
     3781 ACTGGAGCAG AGAGAACTCT ACTTCCTCAG GGCCTTCTCC CAGAGACAGC CTGGCAGAGA 
     3841 AGCCATACTG TTCACAGGTA GATTTCATAT ATAGACAGGA AAGAGGTGGA GGCTCTTCCT 
     3901 TTGATCTCAG AAAGGATGCC TCCCAAAGTG AGGTTTCTGG GGAGAATGAG GGCAACTTGC 
     3961 CATCCCCAGA GGAAGATGGT TTCACTATCT CATTGTCCCA CGTTGCAGTT CCTGGATCCC 
     4021 CAGACCAAAG AGACACAGTC ACCACACCTC TGAGAGAAGT CAGTGCAGAC GGCCCAATCC 
     4081 AGGTGACCAG CACTGTGAAA AACGGTCATG CTGTCCCAGG AGAGGATCCT AGGGGGCAGT 
     4141 TAGGCACGCA TGCTGAATAT GCTTCTGGAC TCATGTCTCC CCTCACCATG TCCCTCCTGG 
     4201 AGAACCCAGA CAACGAAGGG TCTCCTCCCT CGGAGCAGCT GGTCCAGGAT GGGGCTACGC 
     4261 ACAGTCTAGT GGCAGAGAGC ACAGGGGGCC CAGTTGTGAG CCACACAGTG CCATCTGGTG 
     4321 ATCAAGAGGC AGCCTTGCCA GTGTCTTCAG CAACTAGGCA CCTGTGGCTG TCCTCATCTC 
     4381 CCCCTGATAA TAAGCCTGGT GGTGATCTTC CAGCTCTGTC CCCATCACCC ATCCGTCAGC 
     4441 ACCCAGCTGA CAAGCTGCCC AGCAGGGAGG CAGACCTAGG AGAGGCCTGC CAGAGCAGAG 
     4501 AGACTGTACT TTTCTCCCAC GAACACATGG GTAGTGAGCA GTATGATGCT GATGCAGAGG 
     4561 AGACGGGGCT GGATGGCTCC TGGGGTTTCC CAGGAAAGCC CTTCACCACC ATACATATGG 
     4621 GGGTACCCCA TTCTGGACCT ACACTCACCC CACGAACAGG AAGTAGTGAT GTGGCTGACC 
     4681 AGCTCTGGGC CCAGGAGAGA AAACATCCTA CAAGGCTTGG TTGGCAGGAG TTTGGTTTGT 
     4741 CCACAGACCC CATCAAGTTG CCCTGCAACA GTGAAAATGT CACATGGCTC AAACCCAGGC 
     4801 CGATCTCAAG GTGCTTAGCA AGGCCAAGTT CTCCCTTGGT TCCCAGCTGC TCTCCCAAGA 
     4861 CTGCAGGGAC ACTCCGTCAG CCCACCCTGG AGCAAGCGCA GCAGGTGGTC ATCCGAGCAC 
     4921 ACCAGGAACA GCTGGATGAA ATGGCTGAGC TCGGCTTCAA GGAGGAGACG CTGATGAGCC 
     4981 AGCTGGCTTC TAATGATTTT GAAGATTTTG TGACCCAGCT GGATGAAATC ATGGTTCTGA 
     5041 AATCCAAGTG TATCCAGAGT CTGAGGAGCC AGCTGCAGCT CTATCTCACC TGCCACGGGC 
     5101 CCACCGCAGC CCCTGAGGGA ACAGTGCCGT CTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5161 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5221 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5281 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5341 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5401 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5461 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5521 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5581 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5641 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5701 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5761 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5821 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5881 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5941 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6001 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6061 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6121 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6181 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6241 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6301 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6361 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6421 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6481 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6541 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6601 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6661 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6721 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6781 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6841 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6901 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6961 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7021 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7081 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7141 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7201 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7261 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7321 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7381 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7441 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7501 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7561 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7621 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7681 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7741 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7801 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7861 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7921 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7981 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8041 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8101 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8161 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8221 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8281 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8341 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8401 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8461 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8521 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8581 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8641 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8701 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8761 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8821 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8881 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8941 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//