LOCUS       RC220105                9399 bp    DNA     circular  SYN 
DEFINITION  CLASP2 (Myc-DDK-tagged)-Human cytoplasmic linker associated protein 2 (CLASP2), transcript variant 1
ACCESSION   NM_015097
VERSION     NM_015097.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5556..5647 
                     /label="Myc-DDK"  
     misc_feature    5677..6262 
                     /label="PolyA signal"  
     misc_feature    5734..5753 
                     /label="Restriction Enzyme"  
     misc_feature    6538..7210 
                     /label="ColE1"  
     misc_feature    7640..8434 
                     /label="Kan/Neo"  
     misc_feature    8469..8771 
                     /label="SV40 ori"  
     misc_feature    8857..9334 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5538..5544 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5538 
                     /label="CLASP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCCCGC AGCATGGAGT ACTTCTGCGC CCAGGTGCAG CAGAAGGACG 
     1081 TCGGCGGCCG GCTGCAGGTC GGCCAGGAGC TCCTGCTCTA CCTTGGCGCC CCCGGCGCCA 
     1141 TCTCGGACCT GGAGGAGGAC CTGGGCCGCC TAGGCAAGAC AGTCGACGCG CTCACCGGCT 
     1201 GGGTGGGTTC GAGCAACTAC CGGGTATCAT TAATGGGATT GGAAATTTTA AGTGCCTTTG 
     1261 TGGACAGATT ATCAACACGC TTTAAATCCT ATGTAGCAAT GGTTATTGTA GCTTTAATAG 
     1321 ACAGAATGGG AGATGCCAAA GACAAGGTTC GAGATGAAGC TCAGACTCTG ATATTGAAGT 
     1381 TAATGGATCA AGTAGCACCA CCTATGTACA TTTGGGAGCA GTTGGCTTCT GGTTTTAAAC 
     1441 ACAAGAATTT TCGATCTCGA GAAGGCGTGT GTCTGTGTCT TATTGAAACC TTAAACATTT 
     1501 TTGGGGCTCA GCCACTAGTC ATCAGCAAAT TGATACCACA TTTGTGTATC CTGTTTGGAG 
     1561 ACTCCAACAG TCAGGTGAGA GATGCTGCAA TATTGGCTAT AGTGGAGATT TATAGACATG 
     1621 TGGGAGAAAA AGTGAGGATG GATCTTTATA AGAGAGGAAT TCCCCCTGCT AGATTAGAAA 
     1681 TGATATTTGC CAAATTTGAT GAAGTGCAAA GTTCAGGCGG TATGATTTTG AGTGTCTGCA 
     1741 AAGATAAAAG CTTCGATGAT GAAGAATCAG TGGATGGAAA TAGGCCATCA TCAGCTGCAT 
     1801 CAGCCTTCAA GGTTCCTGCA CCTAAAACAT CCGGAAATCC TGCCAACAGT GCAAGGAAGC 
     1861 CTGGTTCAGC AGGTGGCCCT AAGGTTGGAG GTGCTTCTAA GGAAGGAGGT GCTGGAGCAG 
     1921 TTGATGAAGA TGATTTTATA AAAGCTTTTA CAGATGTCCC TTCTATTCAG ATTTATTCTA 
     1981 GTCGAGAACT CGAAGAAACA TTAAATAAAA TCAGGGAAAT TTTGTCAGAT GATAAACATG 
     2041 ACTGGGATCA GCGTGCCAAT GCACTGAAGA AAATTCGATC ACTGCTTGTT GCTGGAGCTG 
     2101 CACAGTATGA TTGCTTTTTT CAACATTTAC GATTGTTGGA TGGAGCACTT AAACTTTCAG 
     2161 CTAAGGATCT TAGATCCCAG GTGGTTAGAG AAGCTTGTAT TACTGTAGCC CACCTTTCAA 
     2221 CAGTTTTGGG AAACAAGTTT GATCATGGCG CTGAAGCCAT TGTACCTACA CTTTTTAATC 
     2281 TCGTCCCCAA TAGTGCAAAA GTCATGGCAA CTTCTGGATG TGCAGCAATC AGATTTATCA 
     2341 TTCGGCATAC TCATGTACCC AGACTTATAC CTTTAATAAC AAGCAATTGC ACATCAAAAT 
     2401 CAGTTCCCGT GAGGAGACGT TCATTTGAAT TTTTAGATTT ATTGTTGCAA GAGTGGCAGA 
     2461 CTCATTCATT GGAAAGACAT GCAGCCGTCT TGGTTGAAAC TATTAAAAAG GGAATTCATG 
     2521 ATGCTGACGC TGAGGCCAGA GTGGAGGCAA GAAAGACATA CATGGGTCTT AGAAACCACT 
     2581 TTCCTGGTGA AGCTGAAACA TTATATAATT CCCTTGAGCC ATCTTATCAG AAGAGTCTTC 
     2641 AAACTTACTT AAAGAGTTCT GGCAGTGTAG CATCTCTTCC ACAATCAGAC AGGTCCTCAT 
     2701 CCAGCTCACA GGAAAGTCTC AATCGCCCTT TTTCTTCCAA ATGGTCTACA GCAAATCCAT 
     2761 CAACTGTGGC TGGAAGAGTA TCAGCAGGCA GCAGCAAAGC CAGTTCCCTT CCAGGAAGCC 
     2821 TGCAGCGTTC ACGAAGTGAC ATTGATGTGA ATGCTGCTGC AGGTGCCAAG GCACATCATG 
     2881 CTGCTGGACA GTCTGTGCGA AGCGGGCGCT TAGGTGCAGG TGCCCTGAAT GCAGGTTCCT 
     2941 ATGCGTCACT AGAGGATACT TCTGACAAGC TGGATGGAAC AGCATCTGAA GATGGCCGGG 
     3001 TGAGAGCAAA ACTTTCAGCA CCACTTGCTG GCATGGGAAA TGCCAAGGCA GATTCTAGAG 
     3061 GAAGAAGTCG AACAAAAATG GTGTCTCAAT CACAGCCTGG TAGCCGGTCT GGGTCTCCAG 
     3121 GAAGAGTTCT GACCACAACA GCCCTGTCCA CTGTGAGCTC TGGTGTTCAA AGAGTCCTGG 
     3181 TCAATTCAGC CTCAGCACAA AAAAGAAGCA AGATACCACG GAGCCAGGGC TGTAGCAGAG 
     3241 AGGCTAGTCC ATCTAGGCTT TCAGTGGCCC GAAGCAGTCG TATTCCTCGA CCAAGTGTGA 
     3301 GTCAAGGATG CAGCCGGGAA GCTAGTCGGG AGAGCAGCAG AGACACAAGT CCTGTTCGCT 
     3361 CTTTTCAGCC CCTCGGTCCA GGTTATGGGA TCAGCCAATC AAGTCGACTG TCGTCTTCTG 
     3421 TTAGTGCCAT GCGAGTCCTG AACACAGGTT CTGATGTGGA GGAGGCGGTG GCAGATGCCT 
     3481 TGAAAAAACC AGCTCGAAGA AGATATGAAT CATATGGAAT GCATTCAGAT GATGACGCCA 
     3541 ACAGCGATGC ATCTAGTGCT TGTTCAGAAC GCTCCTATAG TTCTCGAAAT GGTAGTATTC 
     3601 CTACATATAT GAGGCAGACG GAAGATGTGG CAGAAGTCCT CAATAGATGT GCTAGTTCCA 
     3661 ATTGGTCAGA AAGGAAAGAA GGCCTCCTAG GTCTGCAGAA CTTATTAAAA AATCAGAGAA 
     3721 CACTAAGTCG AGTTGAACTG AAAAGATTAT GTGAAATTTT CACAAGAATG TTTGCTGACC 
     3781 CTCATGGCAA GAGAGTATTC AGCATGTTTT TGGAGACTCT AGTGGATTTC ATACAAGTCC 
     3841 ACAAAGATGA TCTTCAAGAT TGGTTGTTTG TACTGCTGAC ACAACTACTA AAAAAAATGG 
     3901 GTGCTGATTT GCTTGGATCT GTTCAGGCAA AAGTTCAGAA AGCCCTTGAT GTTACAAGAG 
     3961 AGTCTTTTCC AAATGATCTT CAGTTCAATA TTCTAATGAG ATTTACAGTT GATCAGACCC 
     4021 AGACACCAAG CTTAAAGGTG AAGGTTGCTA TCCTTAAATA CATAGAAACT CTGGCCAAAC 
     4081 AGATGGATCC AGGAGATTTT ATAAATTCCA GTGAAACTCG CCTAGCAGTG TCTCGGGTCA 
     4141 TCACTTGGAC AACAGAACCC AAAAGTTCTG ATGTTCGGAA GGCAGCACAG TCAGTGCTGA 
     4201 TTTCATTATT TGAACTCAAT ACCCCAGAGT TTACAATGTT ATTAGGAGCT TTACCAAAAA 
     4261 CTTTTCAGGA TGGTGCTACC AAGCTTCTTC ATAATCACCT TCGAAACACT GGCAATGGAA 
     4321 CCCAGAGTTC CATGGGGAGT CCTTTGACAA GACCAACACC ACGATCACCA GCTAACTGGT 
     4381 CCAGTCCTCT TACTTCTCCT ACCAATACAT CACAGAATAC TTTATCTCCA AGTGCATTTG 
     4441 ATTATGACAC AGAAAATATG AACTCTGAAG ATATTTATAG CTCTCTTAGA GGTGTCACTG 
     4501 AAGCAATCCA GAATTTCAGC TTCCGTAGCC AAGAAGATAT GAATGAGCCA TTGAAAAGGG 
     4561 ATTCTAAAAA AGATGATGGC GATTCAATGT GTGGTGGTCC TGGGATGTCT GACCCAAGAG 
     4621 CAGGAGGTGA TGCTACTGAC TCAAGTCAAA CAGCTCTTGA TAATAAAGCT TCATTGCTCC 
     4681 ATTCAATGCC TACTCACTCC TCTCCACGCT CTCGAGACTA TAATCCATAT AACTATTCAG 
     4741 ATAGCATCAG TCCCTTCAAC AAGTCTGCCC TCAAGGAAGC CATGTTTGAT GATGATGCTG 
     4801 ACCAGTTTCC TGACGATCTT TCCCTAGATC ATTCTGACCT AGTTGCAGAG TTGTTGAAGG 
     4861 AGCTGTCTAA CCATAATGAG CGTGTAGAAG AAAGAAAAAT TGCCCTCTAT GAACTTATGA 
     4921 AACTGACACA GGAAGAATCT TTTAGTGTTT GGGATGAACA CTTCAAAACA ATATTGCTTT 
     4981 TATTGCTTGA AACGCTTGGA GATAAAGAGC CTACAATCAG GGCTTTGGCA TTAAAGGTTT 
     5041 TAAGAGAAAT CCTAAGGCAT CAACCAGCAA GATTTAAAAA CTATGCAGAA TTGACTGTCA 
     5101 TGAAAACATT GGAAGCACAT AAAGATCCTC ATAAGGAGGT GGTGAGATCT GCTGAGGAAG 
     5161 CGGCATCAGT GTTGGCCACT TCAATTAGTC CAGAGCAGTG CATCAAAGTG CTTTGTCCTA 
     5221 TCATTCAAAC TGCAGACTAC CCAATTAATC TGGCTGCAAT CAAAATGCAA ACAAAAGTGA 
     5281 TAGAGAGAGT GTCCAAGGAA ACCCTAAACC TGCTTTTGCC AGAGATTATG CCAGGTCTAA 
     5341 TACAGGGTTA TGATAATTCA GAGAGCAGTG TTCGGAAAGC TTGTGTCTTC TGCCTGGTGG 
     5401 CTGTTCATGC GGTAATTGGT GATGAACTAA AACCACATCT CAGTCAACTT ACTGGCAGTA 
     5461 AAATGAAGCT ACTGAATCTT TACATCAAAC GTGCACAAAC AGGTTCTGGA GGAGCTGATC 
     5521 CCACTACTGA TGTTTCTGGA CAAAGTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5581 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5641 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5701 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5761 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5821 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5881 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5941 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     6001 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     6061 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     6121 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     6181 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     6241 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6301 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6361 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6421 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6481 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6541 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6601 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6661 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6721 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6781 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6841 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6901 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6961 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     7021 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     7081 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     7141 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     7201 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     7261 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7321 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7381 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7441 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7501 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7561 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7621 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7681 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7741 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7801 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7861 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7921 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7981 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     8041 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     8101 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     8161 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     8221 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     8281 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8341 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8401 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8461 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8521 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8581 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8641 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8701 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8761 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8821 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8881 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8941 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     9001 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     9061 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     9121 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     9181 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     9241 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9301 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9361 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//