LOCUS       RC220099                8010 bp    DNA     circular  SYN 
DEFINITION  ATP2A3 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 2
ACCESSION   NM_174955
VERSION     NM_174955.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4167..4258 
                     /label="Myc-DDK"  
     misc_feature    4288..4873 
                     /label="PolyA signal"  
     misc_feature    4345..4364 
                     /label="Restriction Enzyme"  
     misc_feature    5149..5821 
                     /label="ColE1"  
     misc_feature    6251..7045 
                     /label="Kan/Neo"  
     misc_feature    7080..7382 
                     /label="SV40 ori"  
     misc_feature    7468..7945 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4149..4155 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4149 
                     /label="ATP2A3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCGGCG CATCTGCTCC CGGCCGCCGA CGTGCTGCGC CACTTCTCGG 
     1081 TGACAGCCGA GGGCGGCCTG AGCCCGGCGC AGGTGACCGG CGCGCGGGAG CGCTACGGCC 
     1141 CCAACGAGCT CCCGAGTGAG GAAGGGAAGT CCCTGTGGGA GCTGGTGCTG GAACAGTTTG 
     1201 AGGACCTCCT GGTGCGCATC CTGCTGCTGG CTGCCCTTGT CTCCTTTGTC CTGGCCTGGT 
     1261 TCGAGGAGGG CGAGGAGACC ACGACCGCCT TCGTGGAGCC CCTGGTCATC ATGCTGATCC 
     1321 TCGTGGCCAA CGCCATTGTG GGCGTGTGGC AGGAACGCAA CGCCGAGAGT GCCATCGAGG 
     1381 CCCTGAAGGA GTATGAGCCT GAGATGGGCA AGGTGATCCG CTCGGACCGC AAGGGCGTGC 
     1441 AGAGGATCCG TGCCCGGGAC ATCGTCCCAG GGGACATTGT AGAAGTGGCA GTGGGGGACA 
     1501 AAGTGCCTGC TGACCTCCGC CTCATCGAGA TCAAGTCCAC CACGCTGCGA GTGGACCAGT 
     1561 CCATCCTGAC GGGTGAATCT GTGTCCGTGA CCAAGCACAC AGAGGCCATC CCAGACCCCA 
     1621 GAGCTGTGAA CCAGGACAAG AAGAACATGC TGTTTTCTGG CACCAATATC ACATCGGGCA 
     1681 AAGCGGTGGG TGTGGCCGTG GCCACCGGCC TGCACACGGA GCTGGGCAAG ATCCGGAGCC 
     1741 AGATGGCGGC AGTCGAGCCC GAGCGGACGC CGCTGCAGCG CAAGCTGGAC GAGTTTGGAC 
     1801 GGCAGCTGTC CCACGCCATC TCTGTGATCT GCGTGGCCGT GTGGGTCATC AACATCGGCC 
     1861 ACTTCGCCGA CCCGGCCCAC GGTGGCTCCT GGCTGCGTGG CGCTGTCTAC TACTTCAAGA 
     1921 TCGCCGTGGC CCTGGCGGTG GCGGCCATCC CCGAGGGCCT CCCGGCTGTC ATCACTACAT 
     1981 GCCTGGCACT GGGCACGCGG CGCATGGCAC GCAAGAACGC CATCGTGCGA AGCCTGCCGT 
     2041 CCGTGGAGAC CCTGGGCTGC ACCTCAGTCA TCTGCTCCGA CAAGACGGGC ACGCTCACCA 
     2101 CCAATCAGAT GTCTGTCTGC CGGATGTTCG TGGTAGCCGA GGCCGATGCG GGCTCCTGCC 
     2161 TTTTGCACGA GTTCACCATC TCGGGTACCA CGTATACCCC CGAGGGCGAA GTGCGGCAGG 
     2221 GGGATCAGCC TGTGCGCTGC GGCCAGTTCG ACGGGCTGGT GGAGCTGGCG ACCATCTGCG 
     2281 CCCTGTGCAA CGACTCGGCT CTGGACTACA ACGAGGCCAA GGGTGTGTAT GAGAAGGTGG 
     2341 GAGAGGCCAC GGAGACAGCT CTGACTTGCC TGGTGGAGAA GATGAACGTG TTCGACACCG 
     2401 ACCTGCAGGC TCTGTCCCGG GTGGAGCGAG CTGGCGCCTG TAACACGGTC ATCAAGCAGC 
     2461 TGATGCGGAA GGAGTTCACC CTGGAGTTCT CCCGAGACCG GAAATCCATG TCCGTGTACT 
     2521 GCACGCCCAC CCGCCCTCAC CCTACTGGCC AGGGCAGCAA GATGTTTGTG AAGGGGGCTC 
     2581 CTGAGAGTGT GATCGAGCGC TGTAGCTCAG TCCGCGTGGG GAGCCGCACA GCACCCCTGA 
     2641 CCCCCACCTC CAGGGAGCAG ATCCTGGCAA AGATCCGGGA TTGGGGCTCA GGCTCAGACA 
     2701 CGCTGCGCTG CCTGGCACTG GCCACCCGGG ACGCGCCCCC AAGGAAGGAG GACATGGAGC 
     2761 TGGACGACTG CAGCAAGTTT GTGCAGTACG AGACGGACCT GACCTTCGTG GGCTGCGTAG 
     2821 GCATGCTGGA CCCGCCGCGA CCTGAGGTGG CTGCCTGCAT CACACGCTGC TACCAGGCGG 
     2881 GCATCCGCGT GGTCATGATC ACGGGGGATA ACAAAGGCAC TGCCGTGGCC ATCTGCCGCA 
     2941 GGCTTGGCAT CTTTGGGGAC ACGGAAGACG TGGCGGGCAA GGCCTACACG GGCCGCGAGT 
     3001 TTGATGACCT CAGCCCCGAG CAGCAGCGCC AGGCCTGCCG CACCGCCCGC TGCTTCGCCC 
     3061 GCGTGGAGCC CGCACACAAG TCCCGCATCG TGGAGAACCT GCAGTCCTTT AACGAGATCA 
     3121 CTGCTATGAC TGGCGATGGA GTGAACGACG CACCAGCCCT GAAGAAAGCA GAGATCGGCA 
     3181 TCGCCATGGG CTCAGGCACG GCCGTGGCCA AGTCGGCGGC AGAGATGGTG CTGTCAGATG 
     3241 ACAACTTTGC CTCCATCGTG GCTGCGGTGG AGGAGGGCCG GGCCATCTAC AGCAACATGA 
     3301 AGCAATTCAT CCGCTACCTC ATCTCCTCCA ATGTTGGCGA GGTCGTCTGC ATCTTCCTCA 
     3361 CGGCAATTCT GGGCCTGCCC GAAGCCCTGA TCCCTGTGCA GCTGCTCTGG GTGAACCTGG 
     3421 TGACAGACGG CCTACCTGCC ACGGCTCTGG GCTTCAACCC GCCAGACCTG GACATCATGG 
     3481 AGAAGCTGCC CCGGAGCCCC CGAGAAGCCC TCATCAGTGG CTGGCTCTTC TTCCGATACC 
     3541 TGGCTATCGG AGTGTACGTA GGCCTGGCCA CAGTGGCTGC CGCCACCTGG TGGTTTGTGT 
     3601 ATGACGCCGA GGGACCTCAC ATCAACTTCT ACCAGCTGAG GAACTTCCTG AAGTGCTCCG 
     3661 AAGACAACCC GCTCTTTGCC GGCATCGACT GTGAGGTGTT CGAGTCACGC TTCCCCACCA 
     3721 CCATGGCCTT GTCCGTGCTC GTGACCATTG AAATGTGCAA TGCCCTCAAC AGCGTCTCGG 
     3781 AGAACCAGTC GCTGCTGCGG ATGCCGCCCT GGATGAACCC CTGGCTGCTG GTGGCTGTGG 
     3841 CCATGTCCAT GGCCCTGCAC TTCCTCATCC TGCTCGTGCC GCCCCTGCCT CTCATTTTCC 
     3901 AGGTGACCCC ACTGAGCGGG CGCCAGTGGG TGGTGGTGCT CCAGATATCT CTGCCTGTCA 
     3961 TCCTGCTGGA TGAGGCCCTC AAGTACCTGT CCCGGAACCA CATGCACGCC TGTCTTTATC 
     4021 CAGGCCTTCT CAGGACAGTC TCGCAGGCCT GGAGTAGGCA GCCGCTGACC ACCTCTTGGA 
     4081 CCCCAGACCA CACCGGAAGA AATGAGCCAG AAGTGAGCGC TGGGAACAGA GTGGAGTCTC 
     4141 CGGTGTGTAC CTCAGACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4201 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4261 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4321 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4381 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4441 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4501 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4561 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4621 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4681 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4741 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4801 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4861 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4921 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4981 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5041 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5101 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5161 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5221 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5281 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5341 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5401 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5461 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5521 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5581 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5641 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5701 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5761 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5821 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5881 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5941 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6001 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6061 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6121 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6181 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6241 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6301 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6361 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6421 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6481 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6541 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6601 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6661 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6721 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6781 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6841 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6901 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6961 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7021 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7081 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7141 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7201 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7261 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7321 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7381 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7441 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7501 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7561 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7621 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7681 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7741 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7801 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7861 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7921 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7981 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//