LOCUS       RC220061                8364 bp    DNA     circular  SYN 
DEFINITION  MTMR3 (Myc-DDK-tagged)-Human myotubularin related protein 3 (MTMR3), transcript variant 2
ACCESSION   NM_153051
VERSION     NM_153051.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4521..4612 
                     /label="Myc-DDK"  
     misc_feature    4642..5227 
                     /label="PolyA signal"  
     misc_feature    4699..4718 
                     /label="Restriction Enzyme"  
     misc_feature    5503..6175 
                     /label="ColE1"  
     misc_feature    6605..7399 
                     /label="Kan/Neo"  
     misc_feature    7434..7736 
                     /label="SV40 ori"  
     misc_feature    7822..8299 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4503..4509 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4503 
                     /label="MTMR3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAAGAG ACTCGGCACA GCCTTGAGTG CATCCAGGCC AATCAGATCT 
     1081 TTCCCAGGAA GCAGCTGATC CGGGAGGATG AGAATCTTCA GGTTCCTTTC CTTGAACTTC 
     1141 ATGGAGAGAG CACAGAGTTT GTGGGCCGTG CCGAGGATGC CATCATTGCC CTTTCCAATT 
     1201 ACAGACTTCA CATCAAGTTC AAGGAGTCTC TTGTTAATGT TCCATTACAG CTTATAGAAA 
     1261 GTGTTGAATG CCGAGATATA TTTCAGCTTC ATTTGACTTG CAAAGACTGC AAAGTTATCA 
     1321 GGTGTCAGTT TTCAACCTTT GAGCAGTGTC AAGAGTGGCT GAAGAGACTG AACAACGCAA 
     1381 TCCGACCACC TGCTAAAATA GAAGATCTCT TCTCATTTGC ATACCATGCT TGGTGCATGG 
     1441 AGGTCTATGC CAGTGAAAAA GAGCAACATG GAGACCTGTG CAGACCAGGG GAGCATGTAA 
     1501 CTTCAAGGTT TAAAAACGAG GTGGAGAGGA TGGGTTTTGA TATGAACAAC GCCTGGAGGA 
     1561 TTTCCAACAT CAATGAGAAG TACAAATTAT GTGGTAGCTA TCCTCAAGAG CTCATAGTGC 
     1621 CTGCCTGGAT CACTGACAAA GAACTGGAAA GTGTATCAAG TTTCAGGTCC TGGAAGCGCA 
     1681 TCCCTGCCGT CATCTACAGG CACCAGAGCA ATGGAGCTGT CATTGCCCGC TGTGGACAGC 
     1741 CAGAGGTTAG CTGGTGGGGC TGGCGAAATG CAGATGATGA GCATCTGGTA CAGTCAGTAG 
     1801 CCAAAGCTTG TGCCTCTGAC TCCCGATCGA GTGGCAGCAA GCTGTCAACT AGGAACACTT 
     1861 CTCGAGACTT TCCCAATGGG GGAGACCTTT CTGACGTGGA GTTCGATTCT TCTCTGTCAA 
     1921 ATGCTTCAGG AGCAGAGAGT TTAGCCATCC AACCGCAGAA GCTTTTGATC TTGGATGCAC 
     1981 GCTCCTATGC AGCTGCTGTG GCAAACCGAG CCAAAGGAGG AGGCTGCGAA TGCCCAGAGT 
     2041 ATTACCCAAA CTGTGAAGTT GTGTTTATGG GGATGGCAAA CATTCATTCT ATTCGGAGGA 
     2101 GTTTTCAGTC TCTGCGGTTG CTGTGCACTC AGATGCCAGA TCCGGGAAAT TGGCTATCAG 
     2161 CTCTTGAAAG CACAAAATGG CTCCATCACT TGTCTGTGCT TCTGAAATCA GCGCTTCTGG 
     2221 TAGTGCATGC TGTGGATCAG GATCAGCGGC CGGTGCTAGT ACACTGCTCA GATGGCTGGG 
     2281 ACCGCACCCC CCAGATTGTG GCATTGGCTA AGCTCTTGCT GGACCCTTAT TACCGAACCA 
     2341 TAGAGGGTTT CCAGGTCCTC GTGGAAATGG AGTGGCTGGA TTTTGGCCAT AAATTTGCTG 
     2401 ACCGGTGTGG TCATGGGGAG AACTCGGATG ATCTGAATGA ACGTTGCCCA GTGTTTCTGC 
     2461 AGTGGCTTGA CTGTGTTCAT CAGCTTCAGA GGCAATTTCC TTGCTCTTTT GAGTTCAATG 
     2521 AAGCATTCCT TGTGAAACTG GTGCAGCATA CCTATTCCTG CCTGTTTGGA ACATTCCTGT 
     2581 GCAACAACGC CAAGGAGAGA GGGGAAAAGC ATACTCAGGA ACGGACATGT TCCGTGTGGT 
     2641 CACTTCTTCG GGCAGGCAAC AAGGCTTTCA AAAACCTACT GTATTCCTCT CAGTCAGAAG 
     2701 CCGTGCTGTA CCCTGTGTGC CATGTGCGTA ACCTGATGCT GTGGAGTGCA GTGTACCTGC 
     2761 CCTGCCCATC CCCAACCACC CCTGTGGACG ACAGCTGTGC ACCATACCCA GCCCCAGGCA 
     2821 CCAGCCCTGA TGATCCCCCC CTGAGCCGGC TACCAAAGAC TAGATCATAC GACAATCTGA 
     2881 CCACAGCCTG TGACAACACA GTGCCTCTGG CCAGCCGGCG CTGCAGCGAC CCCAGCCTGA 
     2941 ACGAGAAGTG GCAGGAGCAC CGGCGCTCAC TAGAGCTGAG CAGCCTGGCT GGCCCTGGAG 
     3001 AGGATCCCCT TTCTGCCGAC AGCCTAGGGA AGCCCACCAG AGTGCCGGGG GGTGCCGAGC 
     3061 TTTCTGTTGC AGCCGGAGTA GCTGAGGGGC AGATGGAGAA CATCTTGCAG GAGGCCACCA 
     3121 AAGAGGAGAG TGGAGTAGAG GAACCTGCCC ACAGGGCAGG CATTGAGATA CAGGAGGGTA 
     3181 AAGAGGACCC TCTCTTAGAA AAGGAGAGCA GGAGGAAGAC ACCTGAGGCC TCAGCCATTG 
     3241 GACTTCACCA AGACCCAGAA CTGGGTGATG CTGCTCTGAG GAGCCATCTG GATATGAGCT 
     3301 GGCCTCTGTT CTCACAGGGC ATTTCTGAAC AGCAGAGTGG GCTCAGTGTT CTCCTCAGTT 
     3361 CTCTCCAGGT CCCCCCCAGG GGAGAGGATT CCCTGGAGGT CCCTGTGGAG CAGTTTCGAA 
     3421 TAGAAGAGAT TGCAGAGGGT AGGGAGGAAG CAGTTCTTCC AATCCCAGTA GATGCAAAAG 
     3481 TTGGCTATGG TACCTCACAG TCATGTTCTC TGCTACCTTC CCAAGTCCCT TTTGAGACCA 
     3541 GAGGACCAAA CGTGGACAGT TCTACAGACA TGTTAGTGGA AGATAAGGTG AAGTCAGTAA 
     3601 GTGGGCCCCA AGGTCATCAT AGATCTTGCC TTGTAAATAG TGGCAAGGAC AGGCTTCCTC 
     3661 AGACCATGGA ACCCAGCCCT TCAGAGACAA GCCTGGTCGA GAGGCCCCAA GTGGGGTCTG 
     3721 TGGTGCATAG GACTTCCCTT GGCAGCACTC TCAGCCTGAC ACGTTCCCCT TGTGCCTTGC 
     3781 CTTTAGCCGA ATGTAAAGAG GGGCTTGTGT GCAATGGTGC CCCAGAGACT GAAAACAGGG 
     3841 CCTCAGAGCA GCCCCCAGGT CTTAGCACCC TCCAGATGTA CCCCACACCC AATGGGCATT 
     3901 GCGCCAATGG GGAGGCTGGT AGGAGCAAGG ACTCACTGAG CCGTCAGCTG TCTGCTATGA 
     3961 GCTGCAGCTC TGCCCACTTA CACTCAAGGA ACTTGCACCA CAAGTGGCTG CATAGCCACT 
     4021 CAGGAAGGCC ATCTGCAACC AGCAGCCCCG ACCAGCCTTC CCGCAGCCAC CTGGACGATG 
     4081 ATGGCATGTC AGTGTACACA GACACGATCC AACAGCGCCT GCGTCAGATT GAGTCAGGCC 
     4141 ACCAGCAGGA AGTAGAAACT TTGAAGAAAC AAGTCCAGGA GCTGAAGAGT CGCCTGGAGA 
     4201 GCCAGTACCT GACCAGCTCC CTACACTTTA ATGGAGACTT TGGGGATGAG GTGATGACCC 
     4261 GTTGGCTTCC TGACCACCTG GCCGCCCACT GCTATGCGTG CGACAGTGCC TTCTGGCTTG 
     4321 CCAGCAGGAA GCACCACTGC AGGAATTGTG GGAACGTATT CTGCTCCAGT TGTTGTAACC 
     4381 AGAAGGTTCC AGTTCCCAGC CAGCAGCTCT TTGAACCCAG TCGAGTATGC AAGTCTTGCT 
     4441 ATAGCAGCCT ACATCCCACA AGCTCCAGCA TTGACCTTGA ACTGGATAAG CCCATTGCTG 
     4501 CCACTTCCAA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4561 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5341 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5401 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5461 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5521 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5581 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5641 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5701 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5761 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5821 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5881 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5941 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6001 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6061 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6121 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6181 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6241 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6301 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6361 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6421 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6481 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6541 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6601 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6661 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6721 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6781 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6841 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6901 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6961 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7021 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7081 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7141 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7201 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7261 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7321 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7381 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7441 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7501 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7561 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7801 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7861 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7921 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7981 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8041 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8101 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8161 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8221 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8281 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8341 TAACAAAAAT TTAACGCGAA TTTT
//