LOCUS RC220054L4 8981 bp DNA circular SYN DEFINITION GHRHR (Myc-DDK-tagged)-Human growth hormone releasing hormone receptor (GHRHR) ACCESSION NM_000823 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 7272..7452 /label="3-LTR" misc_feature 833..1013 /label="5-LTR" misc_feature 8180..8826 /label="CAT(Cam-r)" misc_feature 2620..3234 /label="CMV" misc_feature 6147..6167 /label="LR50" misc_feature 4610..5338 /label="mGFP" misc_feature 5345..5410 /label="P2A" misc_feature 7500..8119 /label="pBR322 Ori" misc_feature 5411..6010 /label="Puro" misc_feature 3252..3271 /label="T7" misc_feature 3221..3237 /label="V2" misc_feature 3320..3328 /label="Restriction Enzyme" misc_feature 4589..4595 /label="Restriction Enzyme" misc_feature 3320..4589 /label="GHRHR" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGACCGCCGG ATGTGGGGGG CCCACGTCTT 3361 CTGCGTGTTG AGCCCGTTAC CGACCGTATT GGGCCACATG CACCCAGAAT GTGACTTCAT 3421 CACCCAGCTG AGAGAGGATG AGAGTGCCTG TCTACAAGCA GCAGAGGAGA TGCCCAACAC 3481 CACCCTGGGC TGCCCTGCGA CCTGGGATGG GCTGCTGTGC TGGCCAACGG CAGGCTCTGG 3541 CGAGTGGGTC ACCCTCCCCT GCCCGGATTT CTTCTCTCAC TTCAGCTCAG AGTCAGGGGC 3601 TGTGAAACGG GATTGTACTA TCACTGGCTG GTCTGAGCCC TTTCCACCTT ACCCTGTGGC 3661 CTGCCCTGTG CCTCTGGAGC TGCTGGCTGA GGAGGAATCT TACTTCTCCA CAGTGAAGAT 3721 TATCTACACC GTGGGCCATA GCATCTCTAT TGTAGCCCTC TTCGTGGCCA TCACCATCCT 3781 GGTTGCTCTC AGGAGGCTCC ACTGCCCCCG GAACTACGTC CACACCCAGC TGTTCACCAC 3841 TTTTATCCTC AAGGCGGGAG CTGTGTTCCT GAAGGATGCT GCCCTTTTCC ACAGCGACGA 3901 CACTGACCAC TGCAGCTTCT CCACTGTTCT ATGCAAGGTC TCTGTGGCCG CCTCCCATTT 3961 CGCCACCATG ACCAACTTCA GCTGGCTGTT GGCAGAAGCC GTCTACCTGA ACTGCCTCCT 4021 GGCCTCCACC TCCCCCAGCT CAAGGAGAGC CTTCTGGTGG CTGGTTCTCG CTGGCTGGGG 4081 GCTGCCCGTG CTCTTCACTG GCACGTGGGT GAGCTGCAAA CTGGCCTTCG AGGACATCGC 4141 GTGCTGGGAC CTGGACGACA CCTCCCCCTA CTGGTGGATC ATCAAAGGGC CCATTGTCCT 4201 CTCGGTCGGG GTGAACTTTG GGCTTTTTCT CAATATTATC CGCATCCTGG TGAGGAAACT 4261 GGAGCCAGCT CAGGGCAGCC TCCATACCCA GTCTCAGTAT TGGCGTCTCT CCAAGTCGAC 4321 ACTTTTCCTG ATCCCACTCT TTGGAATTCA CTACATCATC TTCAACTTCC TGCCAGACAA 4381 TGCTGGCCTG GGCATCCGCC TCCCCCTGGA GCTGGGACTG GGTTCCTTCC AGGGCTTCAT 4441 TGTTGCCATC CTCTACTGCT TCCTCAACCA AGAGGTGAGG ACTGAGATCT CACGGAAGTG 4501 GCATGGCCAT GACCCTGAGC TTCTGCCAGC CTGGAGGACC CGTGCTAAGT GGACCACGCC 4561 TTCCCGCTCG GCGGCAAAGG TGCTGACATC TATGTGCACG CGTACGCGGC CGCTCGAGAT 4621 GAGCGGGGGC GAGGAGCTGT TCGCCGGCAT CGTGCCCGTG CTGATCGAGC TGGACGGCGA 4681 CGTGCACGGC CACAAGTTCA GCGTGCGCGG CGAGGGCGAG GGCGACGCCG ACTACGGCAA 4741 GCTGGAGATC AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTGGT 4801 GACCACCCTC TGCTACGGCA TCCAGTGCTT CGCCCGCTAC CCCGAGCACA TGAAGATGAA 4861 CGACTTCTTC AAGAGCGCCA TGCCCGAGGG CTACATCCAG GAGCGCACCA TCCAGTTCCA 4921 GGACGACGGC AAGTACAAGA CCCGCGGCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA 4981 CCGCATCGAG CTGAAGGGCA AGGACTTCAA GGAGGACGGC AACATCCTGG GCCACAAGCT 5041 GGAGTACAGC TTCAACAGCC ACAACGTGTA CATCCGCCCC GACAAGGCCA ACAACGGCCT 5101 GGAGGCTAAC TTCAAGACCC GCCACAACAT CGAGGGCGGC GGCGTGCAGC TGGCCGACCA 5161 CTACCAGACC AACGTGCCCC TGGGCGACGG CCCCGTGCTG ATCCCCATCA ACCACTACCT 5221 GAGCACTCAG ACCAAGATCA GCAAGGACCG CAACGAGGCC CGCGACCACA TGGTGCTCCT 5281 GGAGTCCTTC AGCGCCTGCT GCCACACCCA CGGCATGGAC GAGCTGTACA GGTCCGGACT 5341 CAGAGTTTGG GTAGGAAGCG GAGCTACTAA CTTCAGCCTG CTGAAGCAGG CTGGAGACGT 5401 GGAGGAGAAC CCTGGACCTA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA 5461 CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG 5521 CCACACCGTC GATCCGGATC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT 5581 CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC 5641 GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG 5701 CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC 5761 GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA 5821 CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC 5881 CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT 5941 CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC 6001 CCGCAAGCCC GGTGCCTGAT GTACACCCAA ACGGCCGGCC GCGGTCTGTA CAAGTAGGAT 6061 TCGTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA CATGTTTAAG 6121 GGTTCCGGTT CCACTAGGTA CAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT 6181 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG 6241 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT 6301 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC 6361 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA 6421 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC 6481 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT 6541 CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT 6601 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC 6661 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA 6721 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGACCT CGATCGAGAC 6781 CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC TGATTGTGCC 6841 TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG TCACACCTCA GGTACCTTTA 6901 AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA 6961 CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC 7021 ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG GGCCAGGGAT CAGATATCCA 7081 CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC 7141 AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG 7201 GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA CATGGCCCGA 7261 GAGCTGCATC CGGACTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC 7321 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA 7381 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG 7441 TCAGTGTGGA AAATCTCTAG CAGCATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 7501 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 7561 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 7621 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 7681 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 7741 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 7801 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 7861 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 7921 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC 7981 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 8041 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 8101 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 8161 AACTCACGTT AAGGGATTTT GGTCATGATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT 8221 GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT 8281 GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA 8341 CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC 8401 AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT 8461 TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT 8521 GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG 8581 GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG AACTCCGGAT 8641 GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT 8701 TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT 8761 GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG 8821 TGGTATATCC AGTGATTTTT TTCTCCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 8881 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 8941 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA C //