LOCUS       RC220032                8403 bp    DNA     circular  SYN 
DEFINITION  PTPN21 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 21 (PTPN21)
ACCESSION   NM_007039
VERSION     NM_007039.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4560..4651 
                     /label="Myc-DDK"  
     misc_feature    4681..5266 
                     /label="PolyA signal"  
     misc_feature    4738..4757 
                     /label="Restriction Enzyme"  
     misc_feature    5542..6214 
                     /label="ColE1"  
     misc_feature    6644..7438 
                     /label="Kan/Neo"  
     misc_feature    7473..7775 
                     /label="SV40 ori"  
     misc_feature    7861..8338 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4542..4548 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4542 
                     /label="PTPN21"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACTGCCA TTTGGGTTGA AACTGAAACG CACCCGGCGC TACACGGTGT 
     1081 CCAGCAAGAG TTGCCTGGTT GCCCGGATCC AACTGCTTAA TAACGAGTTT GTGGAGTTCA 
     1141 CCCTGTCCGT GGAGAGCACT GGCCAGGAAA GCCTCGAGGC CGTGGCCCAG AGGCTGGAGC 
     1201 TGCGGGAGGT CACTTACTTC AGCCTCTGGT ACTACAACAA GCAAAATCAG CGCCGGTGGG 
     1261 TAGATTTGGA AAAACCTTTG AAGAAGCAGC TGGATAAATA TGCATTGGAA CCTACCGTCT 
     1321 ATTTTGGAGT GGTGTTTTAT GTGCCTTCAG TTTCTCAGCT GCAGCAGGAG ATTACCAGGT 
     1381 ATCAGTATTA TCTGCAACTG AAGAAAGATA TCTTGGAAGG AAGTATTCCT TGTACCTTAG 
     1441 AACAAGCAAT TCAGCTAGCA GGCTTAGCTG TTCAAGCGGA TTTTGGTGAC TTTGATCAGT 
     1501 ATGAATCCCA GGACTTTCTT CAGAAATTTG CCTTGTTTCC TGTGGGATGG TTACAAGATG 
     1561 AAAAAGTATT GGAAGAAGCA ACCCAAAAAG TGGCCTTACT ACATCAGAAA TACAGAGGGC 
     1621 TCACAGCTCC TGATGCTGAA ATGCTGTACA TGCAGGAGGT AGAGAGAATG GATGGCTATG 
     1681 GAGAAGAGAG CTACCCTGCT AAGGATAGCC AAGGAAGTGA CATATCCATT GGAGCGTGTC 
     1741 TTGAAGGTAT CTTTGTGAAA CACAAGAATG GAAGGCATCC TGTGGTATTT AGGTGGCATG 
     1801 ACATTGCCAA CATGTCCCAC AACAAGTCCT TTTTTGCATT AGAGCTGGCA AATAAAGAGG 
     1861 AGACCATTCA ATTTCAAACT GAAGACATGG AAACAGCAAA ATACATTTGG AGACTCTGTG 
     1921 TTGCGCGACA CAAGTTTTAC AGACTAAACC AGTGTAACCT GCAAACTCAG ACTGTCACAG 
     1981 TGAACCCAAT CAGGAGGAGG TCTTCTTCAA GGATGTCTCT GCCTAAACCC CAGCCCTACG 
     2041 TGATGCCTCC CCCACCGCAG TTGCACTATA ATGGACATTA TACAGAACCA TATGCTTCTT 
     2101 CCCAAGATAA CCTCTTTGTG CCCAACCAGA ACGGATACTA CTGTCACTCT CAGACAAGCT 
     2161 TGGATAGAGC CCAGATTGAC CTCAACGGTC GGATCCGTAA TGGCAGTGTC TACAGTGCAC 
     2221 ACAGCACCAA CTCCTTAAAT AATCCTCAGC CCTACTTGCA GCCCTCGCCG ATGTCGTCCA 
     2281 ACCCTAGCAT CACCGGGAGT GACGTCATGA GGCCTGACTA CCTCCCGTCC CATCGGCACA 
     2341 GCGCCGTGAT ACCCCCGTCC TACCGCCCCA CCCCAGACTA TGAGACTGTG ATGAAGCAGC 
     2401 TCAACAGGGG CCTGGTGCAT GCGGAACGGC AGAGCCACTC GCTGCGAAAC CTCAACATCG 
     2461 GCAGCTCGTA CGCCTACAGC AGGCCCGCGG CGCTGGTCTA CAGCCAGCCC GAGATCCGCG 
     2521 AGCACGCACA GCTCCCCTCG CCAGCGGCCG CACACTGCCC GTTCAGCCTG AGCTACAGCT 
     2581 TCCACAGCCC GTCTCCCTAC CCCTACCCTG CCGAGCGGCG GCCCGTGGTG GGCGCGGTCA 
     2641 GCGTGCCGGA GCTGACCAAT GCGCAGCTGC AGGCGCAGGA CTACCCGTCT CCCAACATCA 
     2701 TGCGGACGCA GGTGTACCGG CCACCCCCAC CCTACCCGCC CCCCAGGCCC GCCAACAGCA 
     2761 CGCCAGACCT GTCCCGCCAC CTTTACATCA GCAGCAGCAA CCCCGACCTC ATCACGCGGC 
     2821 GCGTGCACCA CTCGGTGCAA ACGTTCCAGG AGGACAGCCT GCCCGTGGCG CACTCGCTGC 
     2881 AGGAGGTCAG CGAGCCCCTC ACCGCCGCGC GCCACGCGCA GCTGCACAAA CGGAACAGCA 
     2941 TCGAGGTGGC CGGGCTCAGC CACGGCCTGG AGGGCCTGCG GCTCAAGGAG CGCACCCTAT 
     3001 CCGCGTCGGC GGCAGAGGTG GCGCCGCGAG CCGTCTCGGT GGGCTCCCAG CCCAGCGTTT 
     3061 TCACCGAGAG GACACAGCGA GAAGGGCCGG AGGAGGCGGA GGGCTTGAGG TACGGCCATA 
     3121 AGAAGTCCCT GTCGGACGCC ACCATGCTAA TCCACAGCAG CGAGGAGGAG GAGGACGAGG 
     3181 ACTTCGAGGA GGAGAGCGGG GCCCGGGCGC CTCCTGCACG TGCGCGCGAG CCTCGGCCCG 
     3241 GCCTGGCCCA GGACCCACCT GGCTGCCCTC GCGTCCTGCT CGCCGGGCCC CTGCACATCC 
     3301 TGGAGCCCAA GGCCCACGTC CCAGACGCGG AGAAGAGGAT GATGGACAGC AGCCCCGTCC 
     3361 GCACGACCGC AGAGGCCCAG CGGCCCTGGA GAGACGGGCT GCTGATGCCC TCCATGTCGG 
     3421 AGTCCGACCT CACCACGTCA GGCCGCTACC GAGCCCGGAG GGACTCTCTG AAGAAAAGGC 
     3481 CGGTGTCGGA CCTTCTCTCT GGGAAGAAGA ACATCGTGGA AGGGCTCCCG CCTCTAGGGG 
     3541 GAATGAAAAA GACTCGAGTA GATGCAAAAA AAATTGGTCC TCTTAAACTG GCTGCCCTAA 
     3601 ATGGACTCTC CCTATCTCGA GTGCCTCTGC CTGATGAAGG AAAGGAAGTG GCTACCAGAG 
     3661 CAACGAATGA TGAAAGGTGT AAAATTCTGG AACAACGATT AGAACAAGGA ATGGTATTCA 
     3721 CAGAATATGA AAGAATTCTT AAGAAACGGC TAGTTGATGG GGAGTGCTCA ACAGCACGAC 
     3781 TCCCTGAAAA TGCAGAAAGA AATCGATTCC AAGATGTTCT TCCTTATGAT GATGTGAGAG 
     3841 TGGAGTTGGT CCCAACTAAA GAAAACAACA CTGGTTACAT CAACGCATCA CATATTAAGG 
     3901 TCTCTGTCAG TGGAATCGAA TGGGATTATA TTGCCACACA GGGACCATTA CAGAATACCT 
     3961 GTCAAGATTT TTGGCAGATG GTATGGGAAC AGGGAATTGC AATTATAGCA ATGGTGACAG 
     4021 CAGAAGAGGA GGGTGGAAGG GAGAAGAGCT TTAGGTACTG GCCACGACTT GGTTCCAGGC 
     4081 ACAACACTGT CACCTATGGA AGGTTTAAGA TCACGACCCG GTTCCGCACA GACTCTGGCT 
     4141 GCTATGCCAC CACAGGCCTG AAGATGAAGC ACCTCCTTAC TGGGCAAGAG AGGACCGTCT 
     4201 GGCACCTCCA ATACACAGAC TGGCCTGAAC ATGGCTGTCC AGAAGACCTC AAGGGATTTT 
     4261 TATCATATCT TGAAGAGATC CAGTCTGTTC GACGCCATAC AAATAGCACA AGTGATCCCC 
     4321 AAAGCCCCAA CCCTCCGTTG TTGGTCCACT GCAGTGCTGG GGTAGGAAGG ACTGGCGTGG 
     4381 TGATTTTGTC GGAGATCATG ATCGCCTGCC TGGAACACAA TGAGGTGCTG GACATCCCGA 
     4441 GAGTGCTGGA CATGCTGAGG CAACAGAGAA TGATGCTGGT GCAGACTCTC TGCCAGTACA 
     4501 CATTTGTGTA CAGAGTCCTC ATCCAGTTCC TGAAAAGCTC CAGGCTCATC ACGCGTACGC 
     4561 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4621 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4681 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4741 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4801 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4861 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4921 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4981 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5041 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5101 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5161 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5221 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5281 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5341 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5461 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5521 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5581 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5641 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5761 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5821 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5881 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5941 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6061 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6121 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6181 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6241 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6301 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6361 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6421 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6481 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6541 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6601 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6661 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6721 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6781 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6841 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6901 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6961 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7021 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7081 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7141 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7201 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7261 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7321 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7381 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7441 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7501 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7561 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7621 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7801 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7861 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7921 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7981 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8041 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8101 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8161 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8221 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8281 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8341 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8401 TTT
//