LOCUS       RC220010                8994 bp    DNA     circular  SYN 
DEFINITION  ERBB2IP (Myc-DDK-tagged)-Human erbb2 interacting protein (ERBB2IP), transcript variant 2
ACCESSION   NM_018695
VERSION     NM_018695.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5151..5242 
                     /label="Myc-DDK"  
     misc_feature    5272..5857 
                     /label="PolyA signal"  
     misc_feature    5329..5348 
                     /label="Restriction Enzyme"  
     misc_feature    6133..6805 
                     /label="ColE1"  
     misc_feature    7235..8029 
                     /label="Kan/Neo"  
     misc_feature    8064..8366 
                     /label="SV40 ori"  
     misc_feature    8452..8929 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5133..5139 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5133 
                     /label="ERBIN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTACAAAA CGAAGTTTGT TTGTGCGGTT GGTACCATGT CGCTGTCTAC 
     1081 GAGGGGAAGA GGAGACTGTC ACTACTCTTG ATTATTCTCA TTGCAGCTTA GAACAAGTTC 
     1141 CGAAAGAGAT TTTTACTTTT GAAAAAACCT TGGAGGAACT CTATTTAGAT GCTAATCAGA 
     1201 TTGAAGAGCT TCCAAAGCAA CTTTTTAACT GTCAGTCTTT ACACAAACTG AGTTTGCCAG 
     1261 ACAATGATTT AACAACGTTA CCAGCATCCA TTGCAAACCT TATTAATCTC AGGGAACTGG 
     1321 ATGTCAGCAA GAATGGAATA CAGGAGTTTC CAGAAAATAT AAAAAATTGT AAAGTTTTGA 
     1381 CAATTGTGGA GGCCAGTGTA AACCCTATTT CCAAGCTCCC TGATGGATTT TCTCAGCTGT 
     1441 TAAACCTAAC CCAGTTGTAT CTGAATGATG CTTTTCTTGA GTTCTTGCCA GCAAATTTTG 
     1501 GCAGATTAAC TAAACTCCAA ATATTAGAGC TTAGAGAAAA CCAGTTAAAA ATGTTGCCTA 
     1561 AAACTATGAA TAGACTGACC CAGCTGGAAA GATTGGATTT GGGAAGTAAC GAATTCACGG 
     1621 AAGTGCCTGA AGTACTTGAG CAACTAAGTG GATTGAAAGA GTTTTGGATG GATGCTAATA 
     1681 GACTGACTTT TATTCCAGGG TTTATTGGTA GTTTGAAACA GCTCACATAT TTGGATGTTT 
     1741 CTAAAAATAA TATTGAAATG GTTGAAGAAG GAATTTCAAC ATGTGAAAAC CTTCAAGACC 
     1801 TCCTATTATC AAGCAATTCA CTTCAGCAGC TTCCTGAGAC TATTGGTTCG TTGAAGAATA 
     1861 TAACAACGCT TAAAATAGAT GAAAACCAGT TAATGTATCT GCCAGACTCT ATAGGAGGGT 
     1921 TAATATCAGT AGAAGAACTG GATTGTAGTT TCAATGAAGT TGAAGCTTTG CCTTCATCTA 
     1981 TTGGGCAGCT TACTAACTTA AGAACTTTTG CTGCTGATCA TAATTACTTA CAGCAGTTGC 
     2041 CCCCAGAGAT TGGAAGCTGG AAAAATATAA CTGTGCTGTT TCTCCATTCC AATAAACTTG 
     2101 AGACACTTCC AGAGGAAATG GGTGATATGC AAAAATTAAA AGTCATTAAT TTAAGTGATA 
     2161 ATAGATTAAA GAATTTACCC TTTAGCTTTA CAAAGCTACA GCAATTGACA GCTATGTGGC 
     2221 TCTCAGATAA TCAGTCCAAA CCCCTGATAC CTCTTCAAAA AGAAACTGAT TCAGAGACCC 
     2281 AGAAAATGGT GCTTACCAAC TACATGTTCC CTCAACAGCC AAGGACTGAG GATGTTATGT 
     2341 TTATATCAGA TAATGAAAGT TTTAACCCTT CATTGTGGGA GGAACAGAGG AAACAGCGGG 
     2401 CTCAAGTTGC ATTTGAATGT GATGAAGACA AAGATGAAAG GGAGGCACCT CCCAGGGAGG 
     2461 GAAATTTAAA AAGATATCCA ACACCATACC CAGATGAGCT TAAGAATATG GTCAAAACTG 
     2521 TTCAAACCAT TGTACATAGA TTAAAAGATG AAGAGACCAA TGAAGACTCA GGAAGAGATT 
     2581 TGAAACCACA TGAAGATCAA CAAGATATAA ATAAAGATGT GGGTGTGAAG ACCTCAGAAA 
     2641 GTACTACTAC AGTAAAAAGC AAAGTTGATG AAAGAGAAAA ATATATGATA GGAAACTCTG 
     2701 TACAGAAGAT CAGTGAACCT GAAGCTGAGA TTAGTCCTGG GAGTTTACCA GTGACTGCAA 
     2761 ATATGAAAGC CTCTGAGAAC TTGAAGCATA TTGTTAACCA TGATGATGTT TTTGAGGAAT 
     2821 CTGAAGAACT TTCTTCTGAT GAAGAGATGA AAATGGCGGA GATGCGACCA CCATTAATTG 
     2881 AAACCTCTAT TAACCAGCCA AAAGTCGTAG CACTTAGTAA TAACAAAAAA GATGATACAA 
     2941 AGGAAACAGA TTCTTTATCA GATGAAGTTA CACACAATAG CAATCAGAAT AACAGCAATT 
     3001 GTTCTTCTCC ATCTCGGATG TCTGATTCAG TTTCTCTTAA TACTGATAGT AGTCAAGACA 
     3061 CCTCACTCTG CTCTCCAGTG AAACAAACTC ATATTGATAT TAATTCCAAA ATCAGGCAAG 
     3121 AAGATGAAAA TTTTAACAGC CTTTTACAAA ATGGAGATAT TTTAAACAGT TCAACAGAGG 
     3181 AAAAGTTCAA AGCTCATGAT AAAAAAGATT TTAACTTACC TGAATATGAT TTGAATGTTG 
     3241 AAGAGCGATT AGTTCTAATT GAGAAAAGTG TTGACTCAAC AGCCACAGCT GATGACACTC 
     3301 ACAAATTAGA TCATATCAAT ATGAATCTTA ATAAACTTAT AACTAATGAT ACATTTCAAC 
     3361 CAGAGATCAT GGAAAGATCA AAAACACAGG ATATTGTGCT TGGAACAAGC TTTTTAAGCA 
     3421 TTAATTCTAA AGAGGAAACT GAGCACTTGG AAAATGGAAA CAAGTATCCT AATTTGGAAT 
     3481 CCGTAAATAA GGTAAATGGA CATTCTGAGG AAACTTCCCA GTCTCCTAAT AGGACTGAAC 
     3541 CACATGACAG TGATTGTTCT GTTGACTTAG GTATTTCCAA AAGCACTGAA GATCTCTCCC 
     3601 CTCAGAAAAG TGGTCCAGTT GGATCTGTTG TGAAATCTCA TAGCATAACT AATATGGAGA 
     3661 TTGGAGGGCT AAAAATCTAT GATATTCTTA GTGATAATGG ACCTCAGCAG CCAAGTACAA 
     3721 CCGTTAAAAT CACATCTGCT GTTGATGGAA AAAATATAGT CAGGAGCAAG TCTGCCACAC 
     3781 TGTTGTATGA TCAACCATTG CAGGTATTTA CTGGTTCTTC CTCATCTTCT GATTTAATAT 
     3841 CAGGAACAAA GGCAATTTTC AAGTTTGATT CAAATCATAA TCCCGAAGAG CCAAATATAA 
     3901 TAAGAGGCCC CACAAGTGGC CCACAATCTG CACCTCAAAT ATATGGTCCT CCACAGTATA 
     3961 ATATCCAATA CAGTAGCAGT GCTGCAGTCA AAGACACTTT GTGGCACTCC AAACAAAATC 
     4021 CCCAAATAGA CCATGCCAGT TTTCCTCCTC AGCTCCTTCC TAGATCAGAG AGCACAGAAA 
     4081 ATCAAAGTTA TGCTAAACAT TCTGCCAATA TGAATTTCTC TAATCATAAC AATGTTCGAG 
     4141 CTAATACTGC ATACCATTTA CATCAGAGAC TTGGCCCAGC AAGACATGGG GAAATGTGGG 
     4201 CCATCTCACC AAACGACCGA CTTATTCCTG CAGTAACTCG AAGTACAATC CAGCGACAAA 
     4261 GTAGTGTGTC CTCCACAGCC TCTGTAAATC TTGGTGATCC AGGCTCTACA AGGCGGGCTC 
     4321 AGATTCCTGA AGGAGATTAT TTATCATACA GAGAGTTCCA CTCAGCGGGA AGAACTCCTC 
     4381 CAATGATGCC AGGATCACAG AGACCCCTTT CTGCACGAAC ATACAGCATA GATGGTCCAA 
     4441 ATGCATCAAG ACCTCAGAGT GCTCGACCCT CTATTAATGA AATACCAGAG AGAACTATGT 
     4501 CAGTTAGTGA TTTCAATTAT TCACGGACTA GTCCTTCAAA AAGACCAAAT GCAAGGGTTG 
     4561 GTTCTGAGCA TTCTTTATTA GATCCTCCAG GAAAAAGTAA AGTTCCTCGT GACTGGAGAG 
     4621 AACAAGTACT TCGACATATT GAAGCCAAAA AGTTAGAAAA GATGCCTTTG AGTAATGGAC 
     4681 AGATGGGCCA GCCTCTCAGG CCTCAGGCAA ATTATAGTCA AATACATCAC CCCCCTCAGG 
     4741 CATCTGTGGC AAGGCATCCC TCTAGAGAAC AACTAATTGA TTACTTGATG CTGAAAGTGG 
     4801 CCCACCAGCC TCCATATACA CAGCCCCATT GTTCTCCTAG ACAAGGCCAT GAACTGGCAA 
     4861 AACAAGAGAT TCGAGTGAGG GTTGAAAAGG ATCCAGAACT TGGATTTAGC ATATCAGGTG 
     4921 GTGTCGGGGG TAGAGGAAAC CCATTCAGAC CTGATGATGA TGGTATATTT GTAACAAGGG 
     4981 TACAACCTGA AGGACCAGCA TCAAAATTAC TGCAGCCAGG TGATAAAATT ATTCAGGCTA 
     5041 ATGGCTACAG TTTTATAAAT ATTGAACATG GACAAGCAGT GTCCTTGCTA AAAACTTTCC 
     5101 AGAATACAGT TGAACTCATC ATTGTACGAG AAGTTTCCTC AACGCGTACG CGGCCGCTCG 
     5161 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5221 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5281 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5341 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5401 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5461 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5521 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5581 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5641 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5701 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5761 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5821 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5881 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5941 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6001 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6061 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6121 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6181 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6241 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6301 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6361 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6421 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6481 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6541 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6601 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6661 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6721 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6781 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6841 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6901 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6961 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7021 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7081 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7141 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7201 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7261 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7321 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7381 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7441 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7501 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7561 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7621 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7681 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7741 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7801 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7861 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7921 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7981 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8041 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8101 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8161 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8221 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8281 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8341 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8401 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8461 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8521 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8581 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8641 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8701 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8761 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8821 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8881 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8941 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//