LOCUS       RC219966                9417 bp    DNA     circular  SYN 
DEFINITION  EIF4G1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 3
ACCESSION   NM_198244
VERSION     NM_198244.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5574..5665 
                     /label="Myc-DDK"  
     misc_feature    5695..6280 
                     /label="PolyA signal"  
     misc_feature    5752..5771 
                     /label="Restriction Enzyme"  
     misc_feature    6556..7228 
                     /label="ColE1"  
     misc_feature    7658..8452 
                     /label="Kan/Neo"  
     misc_feature    8487..8789 
                     /label="SV40 ori"  
     misc_feature    8875..9352 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5556..5562 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5556 
                     /label="EIF4G1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGATCCCT TCCCAGATCT CCTACCCAGC CTCCCAGGGG GCCTACTACA 
     1081 TCCCTGGACA GGGGCGTTCC ACATACGTTG TCCCGACACA GCAGTACCCT GTGCAGCCAG 
     1141 GAGCCCCAGG CTTCTATCCA GGTGCAAGCC CTACAGAATT TGGGACCTAC GCTGGCGCCT 
     1201 ACTATCCAGC CCAAGGGGTG CAGCAGTTTC CCACTGGCGT GGCCCCCGCC CCAGTTTTGA 
     1261 TGAACCAGCC ACCCCAGATT GCTCCCAAGA GGGAGCGTAA GACGATCCGA ATTCGAGATC 
     1321 CAAACCAAGG AGGAAAGGAT ATCACAGAGG AGATCATGTC TGGGGCCCGC ACTGCCTCCA 
     1381 CACCCACCCC TCCCCAGACG GGAGGCGGTC TGGAGCCTCA AGCTAATGGG GAGACGCCCC 
     1441 AGGTTGCTGT CATTGTCCGG CCAGATGACC GGTCACAGGG AGCAATCATT GCTGACCGGC 
     1501 CAGGGCTGCC TGGCCCAGAG CATAGCCCTT CAGAATCCCA GCCTTCGTCG CCTTCTCCGA 
     1561 CCCCATCACC ATCCCCAGTC TTGGAACCGG GGTCTGAGCC TAATCTCGCA GTCCTCTCTA 
     1621 TTCCTGGGGA CACTATGACA ACTATACAAA TGTCTGTAGA AGAATCAACC CCCATCTCCC 
     1681 GTGAAACTGG GGAGCCATAT CGCCTCTCTC CAGAACCCAC TCCTCTCGCC GAACCCATAC 
     1741 TGGAAGTAGA AGTGACACTT AGCAAACCGG TTCCAGAATC TGAGTTTTCT TCCAGTCCTC 
     1801 TCCAGGCTCC CACCCCTTTG GCATCTCACA CAGTGGAAAT TCATGAGCCT AATGGCATGG 
     1861 TCCCATCTGA AGATCTGGAA CCAGAGGTGG AGTCAAGCCC AGAGCTTGCT CCTCCCCCAG 
     1921 CTTGCCCCTC CGAATCCCCT GTGCCCATTG CTCCAACTGC CCAACCTGAG GAACTGCTCA 
     1981 ACGGAGCCCC CTCGCCACCA GCTGTGGACT TAAGCCCAGT CAGTGAGCCA GAGGAGCAGG 
     2041 CCAAGGAGGT GACAGCATCA ATGGCGCCCC CCACCATCCC CTCTGCTACT CCAGCTACGG 
     2101 CTCCTTCAGC TACTTCCCCA GCTCAGGAGG AGGAAATGGA AGAAGAAGAA GAAGAGGAAG 
     2161 AAGGAGAAGC AGGAGAAGCA GGAGAAGCTG AGAGTGAGAA AGGAGGAGAG GAACTGCTCC 
     2221 CCCCAGAGAG TACCCCTATT CCAGCCAACT TGTCTCAGAA TTTGGAGGCA GCAGCAGCCA 
     2281 CTCAAGTGGC AGTATCTGTG CCAAAGAGGA GACGGAAAAT TAAGGAGCTA AATAAGAAGG 
     2341 AGGCTGTTGG AGACCTTCTG GATGCCTTCA AGGAGGCGAA CCCGGCAGTA CCAGAGGTGG 
     2401 AAAATCAGCC TCCTGCAGGC AGCAATCCAG GCCCAGAGTC TGAGGGCAGT GGTGTGCCCC 
     2461 CACGTCCTGA GGAAGCAGAT GAGACCTGGG ACTCAAAGGA AGACAAAATT CACAATGCTG 
     2521 AGAACATCCA GCCCGGGGAA CAGAAGTATG AATATAAGTC AGATCAGTGG AAGCCTCTAA 
     2581 ACCTAGAGGA GAAAAAACGT TACGACCGTG AGTTCCTGCT TGGTTTTCAG TTCATCTTTG 
     2641 CCAGTATGCA GAAGCCAGAG GGATTGCCAC ATATCAGTGA CGTGGTGCTG GACAAGGCCA 
     2701 ATAAAACACC ACTGCGGCCA CTGGATCCCA CTAGACTACA AGGCATAAAT TGTGGCCCAG 
     2761 ACTTCACTCC ATCCTTTGCC AACCTTGGCC GGACAACCCT TAGCACCCGT GGGCCCCCAA 
     2821 GGGGTGGGCC AGGTGGGGAG CTGCCCCGTG GGCCGGCTGG CCTGGGACCC CGGCGCTCTC 
     2881 AGCAGGGACC CCGAAAAGAA CCACGCAAGA TCATTGCCAC AGTGTTAATG ACCGAAGATA 
     2941 TAAAACTGAA CAAAGCAGAG AAAGCCTGGA AACCCAGCAG CAAGCGGACG GCGGCTGATA 
     3001 AGGATCGAGG GGAAGAAGAT GCTGATGGCA GCAAAACCCA GGACCTATTC CGCAGGGTGC 
     3061 GCTCCATCCT GAATAAACTG ACACCCCAGA TGTTCCAGCA GCTGATGAAG CAAGTGACGC 
     3121 AGCTGGCCAT CGACACCGAG GAACGCCTCA AAGGGGTCAT TGACCTCATT TTTGAGAAGG 
     3181 CCATTTCAGA GCCCAACTTC TCTGTGGCCT ATGCCAACAT GTGCCGCTGC CTCATGGCGC 
     3241 TGAAAGTGCC CACTACGGAA AAGCCAACAG TGACTGTGAA CTTCCGAAAG CTGTTGTTGA 
     3301 ATCGATGTCA GAAGGAGTTT GAGAAAGACA AAGATGATGA TGAGGTTTTT GAGAAGAAGC 
     3361 AAAAAGAGAT GGATGAAGCT GCTACGGCAG AGGAACGAGG ACGCCTGAAG GAAGAGCTGG 
     3421 AAGAGGCTCG GGACATAGCC CGGCGGCGCT CTTTAGGGAA TATCAAGTTT ATTGGAGAGT 
     3481 TGTTCAAACT GAAGATGTTA ACAGAGGCAA TAATGCATGA CTGTGTGGTC AAACTGCTTA 
     3541 AGAACCATGA TGAAGAGTCC CTTGAGTGCC TTTGTCGTCT GCTCACCACC ATTGGCAAAG 
     3601 ACCTGGACTT TGAAAAAGCC AAGCCCCGAA TGGATCAGTA TTTCAACCAG ATGGAAAAAA 
     3661 TCATTAAAGA AAAGAAGACG TCATCCCGCA TCCGCTTTAT GCTGCAGGAC GTGCTGGATC 
     3721 TGCGAGGGAG CAATTGGGTG CCACGCCGAG GGGATCAGGG TCCCAAGACC ATTGACCAGA 
     3781 TCCATAAGGA GGCTGAGATG GAAGAACATC GAGAGCACAT CAAAGTGCAG CAGCTCATGG 
     3841 CCAAGGGCAG TGACAAGCGT CGGGGCGGTC CTCCAGGCCC TCCCATCAGC CGTGGACTTC 
     3901 CCCTTGTGGA TGATGGTGGC TGGAACACAG TTCCCATCAG CAAAGGTAGC CGCCCCATTG 
     3961 ACACCTCACG ACTCACCAAG ATCACCAAGC CTGGCTCCAT CGATTCTAAC AACCAGCTCT 
     4021 TTGCACCTGG AGGGCGACTG AGCTGGGGCA AGGGCAGCAG CGGAGGCTCA GGAGCCAAGC 
     4081 CCTCAGACGC AGCATCAGAA GCTGCTCGCC CAGCTACTAG TACTTTGAAT CGCTTCTCAG 
     4141 CCCTTCAACA AGCGGTACCC ACAGAAAGCA CAGATAATAG ACGTGTGGTG CAGAGGAGTA 
     4201 GCTTGAGCCG AGAACGAGGC GAGAAAGCTG GAGACCGAGG AGACCGCCTA GAGCGGAGTG 
     4261 AACGGGGAGG GGACCGTGGG GACCGGCTTG ATCGTGCGCG GACACCTGCT ACCAAGCGGA 
     4321 GCTTCAGCAA GGAAGTGGAG GAGCGGAGTA GAGAACGGCC CTCCCAGCCT GAGGGGCTGC 
     4381 GCAAGGCAGC TAGCCTCACG GAGGATCGGG ACCGTGGGCG GGATGCCGTG AAGCGAGAAG 
     4441 CTGCCCTACC CCCAGTGAGC CCCCTGAAGG CGGCTCTCTC TGAGGAGGAG TTAGAGAAGA 
     4501 AATCCAAGGC TATCATTGAG GAATATCTCC ATCTCAATGA CATGAAAGAG GCAGTCCAGT 
     4561 GCGTGCAGGA GCTGGCCTCA CCCTCCTTGC TCTTCATCTT TGTACGGCAT GGTGTCGAGT 
     4621 CTACGCTGGA GCGCAGTGCC ATTGCTCGTG AGCATATGGG GCAGCTGCTG CACCAGCTGC 
     4681 TCTGTGCTGG GCATCTGTCT ACTGCTCAGT ACTACCAAGG GTTGTATGAA ATCTTGGAAT 
     4741 TGGCTGAGGA CATGGAAATT GACATCCCCC ACGTGTGGCT CTACCTAGCG GAACTGGTAA 
     4801 CACCCATTCT GCAGGAAGGT GGGGTGCCCA TGGGGGAGCT GTTCAGGGAG ATTACAAAGC 
     4861 CTCTGAGACC GTTGGGCAAA GCTGCTTCCC TGTTGCTGGA GATCCTGGGC CTCCTGTGCA 
     4921 AAAGCATGGG TCCTAAAAAG GTGGGGACGC TGTGGCGAGA AGCCGGGCTT AGCTGGAAGG 
     4981 AATTTCTACC TGAAGGCCAG GACATTGGTG CATTCGTCGC TGAACAGAAG GTGGAGTATA 
     5041 CCCTGGGAGA GGAGTCGGAA GCCCCTGGCC AGAGGGCACT CCCCTCCGAG GAGCTGAACA 
     5101 GGCAGCTGGA GAAGCTGCTG AAGGAGGGCA GCAGTAACCA GCGGGTGTTC GACTGGATAG 
     5161 AGGCCAACCT GAGTGAGCAG CAGATAGTAT CCAACACGTT AGTTCGAGCC CTCATGACGG 
     5221 CTGTCTGCTA TTCTGCAATT ATTTTTGAGA CTCCCCTCCG AGTGGACGTT GCAGTGCTGA 
     5281 AAGCGCGAGC GAAGCTGCTG CAGAAATACC TGTGTGACGA GCAGAAGGAG CTACAGGCGC 
     5341 TCTACGCCCT CCAGGCCCTT GTAGTGACCT TAGAACAGCC TCCCAACCTG CTGCGGATGT 
     5401 TCTTTGACGC ACTGTATGAC GAGGACGTGG TGAAGGAGGA TGCCTTCTAC AGTTGGGAGA 
     5461 GTAGCAAGGA CCCCGCTGAG CAGCAGGGCA AGGGTGTGGC CCTTAAATCT GTCACAGCCT 
     5521 TCTTCAAGTG GCTCCGTGAA GCAGAGGAGG AGTCTGACCA CAACACGCGT ACGCGGCCGC 
     5581 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5641 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5701 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5761 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5821 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5881 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5941 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     6001 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     6061 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6121 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6181 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6241 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6301 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6361 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6421 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6481 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6541 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6601 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6661 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6721 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6781 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6841 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6901 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6961 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     7021 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     7081 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7141 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7201 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7261 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7321 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7381 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7441 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7501 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7561 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7621 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7681 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7741 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7801 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7861 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7921 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7981 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     8041 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     8101 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8161 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8221 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8281 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8341 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8401 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8461 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8521 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8581 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8641 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8701 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8761 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8821 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8881 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8941 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     9001 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     9061 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9121 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9181 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9241 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9301 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9361 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//