LOCUS       RC219910                9186 bp    DNA     circular  SYN 
DEFINITION  EIF4G1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 4
ACCESSION   NM_198242
VERSION     NM_198242.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5343..5434 
                     /label="Myc-DDK"  
     misc_feature    5464..6049 
                     /label="PolyA signal"  
     misc_feature    5521..5540 
                     /label="Restriction Enzyme"  
     misc_feature    6325..6997 
                     /label="ColE1"  
     misc_feature    7427..8221 
                     /label="Kan/Neo"  
     misc_feature    8256..8558 
                     /label="SV40 ori"  
     misc_feature    8644..9121 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5325..5331 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5325 
                     /label="EIF4G1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCAGCCA CCCCAGATTG CTCCCAAGAG GGAGCGTAAG ACGATCCGAA 
     1081 TTCGAGATCC AAACCAAGGA GGAAAGGATA TCACAGAGGA GATCATGTCT GGGGCCCGCA 
     1141 CTGCCTCCAC ACCCACCCCT CCCCAGACGG GAGGCGGTCT GGAGCCTCAA GCTAATGGGG 
     1201 AGACGCCCCA GGTTGCTGTC ATTGTCCGGC CAGATGACCG GTCACAGGGA GCAATCATTG 
     1261 CTGACCGGCC AGGGCTGCCT GGCCCAGAGC ATAGCCCTTC AGAATCCCAG CCTTCGTCGC 
     1321 CTTCTCCGAC CCCATCACCA TCCCCAGTCT TGGAACCGGG GTCTGAGCCT AATCTCGCAG 
     1381 TCCTCTCTAT TCCTGGGGAC ACTATGACAA CTATACAAAT GTCTGTAGAA GAATCAACCC 
     1441 CCATCTCCCG TGAAACTGGG GAGCCATATC GCCTCTCTCC AGAACCCACT CCTCTCGCCG 
     1501 AACCCATACT GGAAGTAGAA GTGACACTTA GCAAACCGGT TCCAGAATCT GAGTTTTCTT 
     1561 CCAGTCCTCT CCAGGCTCCC ACCCCTTTGG CATCTCACAC AGTGGAAATT CATGAGCCTA 
     1621 ATGGCATGGT CCCATCTGAA GATCTGGAAC CAGAGGTGGA GTCAAGCCCA GAGCTTGCTC 
     1681 CTCCCCCAGC TTGCCCCTCC GAATCCCCTG TGCCCATTGC TCCAACTGCC CAACCTGAGG 
     1741 AACTGCTCAA CGGAGCCCCC TCGCCACCAG CTGTGGACTT AAGCCCAGTC AGTGAGCCAG 
     1801 AGGAGCAGGC CAAGGAGGTG ACAGCATCAA TGGCGCCCCC CACCATCCCC TCTGCTACTC 
     1861 CAGCTACGGC TCCTTCAGCT ACTTCCCCAG CTCAGGAGGA GGAAATGGAA GAAGAAGAAG 
     1921 AAGAGGAAGA AGGAGAAGCA GGAGAAGCAG GAGAAGCTGA GAGTGAGAAA GGAGGAGAGG 
     1981 AACTGCTCCC CCCAGAGAGT ACCCCTATTC CAGCCAACTT GTCTCAGAAT TTGGAGGCAG 
     2041 CAGCAGCCAC TCAAGTGGCA GTATCTGTGC CAAAGAGGAG ACGGAAAATT AAGGAGCTAA 
     2101 ATAAGAAGGA GGCTGTTGGA GACCTTCTGG ATGCCTTCAA GGAGGCGAAC CCGGCAGTAC 
     2161 CAGAGGTGGA AAATCAGCCT CCTGCAGGCA GCAATCCAGG CCCAGAGTCT GAGGGCAGTG 
     2221 GTGTGCCCCC ACGTCCTGAG GAAGCAGATG AGACCTGGGA CTCAAAGGAA GACAAAATTC 
     2281 ACAATGCTGA GAACATCCAG CCCGGGGAAC AGAAGTATGA ATATAAGTCA GATCAGTGGA 
     2341 AGCCTCTAAA CCTAGAGGAG AAAAAACGTT ACGACCGTGA GTTCCTGCTT GGTTTTCAGT 
     2401 TCATCTTTGC CAGTATGCAG AAGCCAGAGG GATTGCCACA TATCAGTGAC GTGGTGCTGG 
     2461 ACAAGGCCAA TAAAACACCA CTGCGGCCAC TGGATCCCAC TAGACTACAA GGCATAAATT 
     2521 GTGGCCCAGA CTTCACTCCA TCCTTTGCCA ACCTTGGCCG GACAACCCTT AGCACCCGTG 
     2581 GGCCCCCAAG GGGTGGGCCA GGTGGGGAGC TGCCCCGTGG GCCGGCTGGC CTGGGACCCC 
     2641 GGCGCTCTCA GCAGGGACCC CGAAAAGAAC CACGCAAGAT CATTGCCACA GTGTTAATGA 
     2701 CCGAAGATAT AAAACTGAAC AAAGCAGAGA AAGCCTGGAA ACCCAGCAGC AAGCGGACGG 
     2761 CGGCTGATAA GGATCGAGGG GAAGAAGATG CTGATGGCAG CAAAACCCAG GACCTATTCC 
     2821 GCAGGGTGCG CTCCATCCTG AATAAACTGA CACCCCAGAT GTTCCAGCAG CTGATGAAGC 
     2881 AAGTGACGCA GCTGGCCATC GACACCGAGG AACGCCTCAA AGGGGTCATT GACCTCATTT 
     2941 TTGAGAAGGC CATTTCAGAG CCCAACTTCT CTGTGGCCTA TGCCAACATG TGCCGCTGCC 
     3001 TCATGGCGCT GAAAGTGCCC ACTACGGAAA AGCCAACAGT GACTGTGAAC TTCCGAAAGC 
     3061 TGTTGTTGAA TCGATGTCAG AAGGAGTTTG AGAAAGACAA AGATGATGAT GAGGTTTTTG 
     3121 AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGAGGA CGCCTGAAGG 
     3181 AAGAGCTGGA AGAGGCTCGG GACATAGCCC GGCGGCGCTC TTTAGGGAAT ATCAAGTTTA 
     3241 TTGGAGAGTT GTTCAAACTG AAGATGTTAA CAGAGGCAAT AATGCATGAC TGTGTGGTCA 
     3301 AACTGCTTAA GAACCATGAT GAAGAGTCCC TTGAGTGCCT TTGTCGTCTG CTCACCACCA 
     3361 TTGGCAAAGA CCTGGACTTT GAAAAAGCCA AGCCCCGAAT GGATCAGTAT TTCAACCAGA 
     3421 TGGAAAAAAT CATTAAAGAA AAGAAGACGT CATCCCGCAT CCGCTTTATG CTGCAGGACG 
     3481 TGCTGGATCT GCGAGGGAGC AATTGGGTGC CACGCCGAGG GGATCAGGGT CCCAAGACCA 
     3541 TTGACCAGAT CCATAAGGAG GCTGAGATGG AAGAACATCG AGAGCACATC AAAGTGCAGC 
     3601 AGCTCATGGC CAAGGGCAGT GACAAGCGTC GGGGCGGTCC TCCAGGCCCT CCCATCAGCC 
     3661 GTGGACTTCC CCTTGTGGAT GATGGTGGCT GGAACACAGT TCCCATCAGC AAAGGTAGCC 
     3721 GCCCCATTGA CACCTCACGA CTCACCAAGA TCACCAAGCC TGGCTCCATC GATTCTAACA 
     3781 ACCAGCTCTT TGCACCTGGA GGGCGACTGA GCTGGGGCAA GGGCAGCAGC GGAGGCTCAG 
     3841 GAGCCAAGCC CTCAGACGCA GCATCAGAAG CTGCTCGCCC AGCTACTAGT ACTTTGAATC 
     3901 GCTTCTCAGC CCTTCAACAA GCGGTACCCA CAGAAAGCAC AGATAATAGA CGTGTGGTGC 
     3961 AGAGGAGTAG CTTGAGCCGA GAACGAGGCG AGAAAGCTGG AGACCGAGGA GACCGCCTAG 
     4021 AGCGGAGTGA ACGGGGAGGG GACCGTGGGG ACCGGCTTGA TCGTGCGCGG ACACCTGCTA 
     4081 CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AGCGGAGTAG AGAACGGCCC TCCCAGCCTG 
     4141 AGGGGCTGCG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGTGGGCGG GATGCCGTGA 
     4201 AGCGAGAAGC TGCCCTACCC CCAGTGAGCC CCCTGAAGGC GGCTCTCTCT GAGGAGGAGT 
     4261 TAGAGAAGAA ATCCAAGGCT ATCATTGAGG AATATCTCCA TCTCAATGAC ATGAAAGAGG 
     4321 CAGTCCAGTG CGTGCAGGAG CTGGCCTCAC CCTCCTTGCT CTTCATCTTT GTACGGCATG 
     4381 GTGTCGAGTC TACGCTGGAG CGCAGTGCCA TTGCTCGTGA GCATATGGGG CAGCTGCTGC 
     4441 ACCAGCTGCT CTGTGCTGGG CATCTGTCTA CTGCTCAGTA CTACCAAGGG TTGTATGAAA 
     4501 TCTTGGAATT GGCTGAGGAC ATGGAAATTG ACATCCCCCA CGTGTGGCTC TACCTAGCGG 
     4561 AACTGGTAAC ACCCATTCTG CAGGAAGGTG GGGTGCCCAT GGGGGAGCTG TTCAGGGAGA 
     4621 TTACAAAGCC TCTGAGACCG TTGGGCAAAG CTGCTTCCCT GTTGCTGGAG ATCCTGGGCC 
     4681 TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAA GCCGGGCTTA 
     4741 GCTGGAAGGA ATTTCTACCT GAAGGCCAGG ACATTGGTGC ATTCGTCGCT GAACAGAAGG 
     4801 TGGAGTATAC CCTGGGAGAG GAGTCGGAAG CCCCTGGCCA GAGGGCACTC CCCTCCGAGG 
     4861 AGCTGAACAG GCAGCTGGAG AAGCTGCTGA AGGAGGGCAG CAGTAACCAG CGGGTGTTCG 
     4921 ACTGGATAGA GGCCAACCTG AGTGAGCAGC AGATAGTATC CAACACGTTA GTTCGAGCCC 
     4981 TCATGACGGC TGTCTGCTAT TCTGCAATTA TTTTTGAGAC TCCCCTCCGA GTGGACGTTG 
     5041 CAGTGCTGAA AGCGCGAGCG AAGCTGCTGC AGAAATACCT GTGTGACGAG CAGAAGGAGC 
     5101 TACAGGCGCT CTACGCCCTC CAGGCCCTTG TAGTGACCTT AGAACAGCCT CCCAACCTGC 
     5161 TGCGGATGTT CTTTGACGCA CTGTATGACG AGGACGTGGT GAAGGAGGAT GCCTTCTACA 
     5221 GTTGGGAGAG TAGCAAGGAC CCCGCTGAGC AGCAGGGCAA GGGTGTGGCC CTTAAATCTG 
     5281 TCACAGCCTT CTTCAAGTGG CTCCGTGAAG CAGAGGAGGA GTCTGACCAC AACACGCGTA 
     5341 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5401 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5461 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5521 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5581 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5641 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5701 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5761 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5821 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5881 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5941 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     6001 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     6061 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6121 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6181 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6241 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6301 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6361 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6421 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6481 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6541 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6601 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6661 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6721 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6781 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6841 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6901 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6961 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     7021 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     7081 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7141 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7201 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7261 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7321 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7381 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7441 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7501 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7561 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7621 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7681 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7741 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7801 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7861 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7921 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7981 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     8041 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     8101 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8161 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8221 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8281 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8341 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8401 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8461 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8521 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8581 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8641 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8701 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8761 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8821 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8881 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8941 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     9001 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     9061 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9121 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9181 AATTTT
//