LOCUS RC219869 6909 bp DNA circular SYN DEFINITION KCNQ1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 1 (KCNQ1), transcript variant 1 ACCESSION NM_000218 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3066..3157 /label="Myc-DDK" misc_feature 3187..3772 /label="PolyA signal" misc_feature 3244..3263 /label="Restriction Enzyme" misc_feature 4048..4720 /label="ColE1" misc_feature 5150..5944 /label="Kan/Neo" misc_feature 5979..6281 /label="SV40 ori" misc_feature 6367..6844 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3048..3054 /label="Restriction Enzyme" misc_feature 1020..3048 /label="KCNQ1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCCGCGGCC TCCTCCCCGC CCAGGGCCGA GAGGAAGCGC TGGGGTTGGG 1081 GCCGCCTGCC AGGCGCCCGC CGGGGCAGCG CGGGCCTGGC CAAGAAGTGC CCCTTCTCGC 1141 TGGAGCTGGC GGAGGGCGGC CCGGCGGGCG GCGCGCTCTA CGCGCCCATC GCGCCCGGCG 1201 CCCCAGGTCC CGCGCCCCCT GCGTCCCCGG CCGCGCCCGC CGCGCCCCCA GTTGCCTCCG 1261 ACCTTGGCCC GCGGCCGCCG GTGAGCCTAG ACCCGCGCGT CTCCATCTAC AGCACGCGCC 1321 GCCCGGTGTT GGCGCGCACC CACGTCCAGG GCCGCGTCTA CAACTTCCTC GAGCGTCCCA 1381 CCGGCTGGAA ATGCTTCGTT TACCACTTCG CCGTCTTCCT CATCGTCCTG GTCTGCCTCA 1441 TCTTCAGCGT GCTGTCCACC ATCGAGCAGT ATGCCGCCCT GGCCACGGGG ACTCTCTTCT 1501 GGATGGAGAT CGTGCTGGTG GTGTTCTTCG GGACGGAGTA CGTGGTCCGC CTCTGGTCCG 1561 CCGGCTGCCG CAGCAAGTAC GTGGGCCTCT GGGGGCGGCT GCGCTTTGCC CGGAAGCCCA 1621 TTTCCATCAT CGACCTCATC GTGGTCGTGG CCTCCATGGT GGTCCTCTGC GTGGGCTCCA 1681 AGGGGCAGGT GTTTGCCACG TCGGCCATCA GGGGCATCCG CTTCCTGCAG ATCCTGAGGA 1741 TGCTACACGT CGACCGCCAG GGAGGCACCT GGAGGCTCCT GGGCTCCGTG GTCTTCATCC 1801 ACCGCCAGGA GCTGATAACC ACCCTGTACA TCGGCTTCCT GGGCCTCATC TTCTCCTCGT 1861 ACTTTGTGTA CCTGGCTGAG AAGGACGCGG TGAACGAGTC AGGCCGCGTG GAGTTCGGCA 1921 GCTACGCAGA TGCGCTGTGG TGGGGGGTGG TCACAGTCAC CACCATCGGC TATGGGGACA 1981 AGGTGCCCCA GACGTGGGTC GGGAAGACCA TCGCCTCCTG CTTCTCTGTC TTTGCCATCT 2041 CCTTCTTTGC GCTCCCAGCG GGGATTCTTG GCTCGGGGTT TGCCCTGAAG GTGCAGCAGA 2101 AGCAGAGGCA GAAGCACTTC AACCGGCAGA TCCCGGCGGC AGCCTCACTC ATTCAGACCG 2161 CATGGAGGTG CTATGCTGCC GAGAACCCCG ACTCCTCCAC CTGGAAGATC TACATCCGGA 2221 AGGCCCCCCG GAGCCACACT CTGCTGTCAC CCAGCCCCAA ACCCAAGAAG TCTGTGGTGG 2281 TAAAGAAAAA AAAGTTCAAG CTGGACAAAG ACAATGGGGT GACTCCTGGA GAGAAGATGC 2341 TCACAGTCCC CCATATCACG TGCGACCCCC CAGAAGAGCG GCGGCTGGAC CACTTCTCTG 2401 TCGACGGCTA TGACAGTTCT GTAAGGAAGA GCCCAACACT GCTGGAAGTG AGCATGCCCC 2461 ATTTCATGAG AACCAACAGC TTCGCCGAGG ACCTGGACCT GGAAGGGGAG ACTCTGCTGA 2521 CACCCATCAC CCACATCTCA CAGCTGCGGG AACACCATCG GGCCACCATT AAGGTCATTC 2581 GACGCATGCA GTACTTTGTG GCCAAGAAGA AATTCCAGCA AGCGCGGAAG CCTTACGATG 2641 TGCGGGACGT CATTGAGCAG TACTCGCAGG GCCACCTCAA CCTCATGGTG CGCATCAAGG 2701 AGCTGCAGAG GAGGCTGGAC CAGTCCATTG GGAAGCCCTC ACTGTTCATC TCCGTCTCAG 2761 AAAAGAGCAA GGATCGCGGC AGCAACACGA TCGGCGCCCG CCTGAACCGA GTAGAAGACA 2821 AGGTGACGCA GCTGGACCAG AGGCTGGCAC TCATCACCGA CATGCTTCAC CAGCTGCTCT 2881 CCTTGCACGG TGGCAGCACC CCCGGCAGCG GCGGCCCCCC CAGAGAGGGC GGGGCCCACA 2941 TCACCCAGCC CTGCGGCAGT GGCGGCTCCG TCGACCCTGA GCTCTTCCTG CCCAGCAACA 3001 CCCTGCCCAC CTACGAGCAG CTGACCGTGC CCAGGAGGGG CCCCGATGAG GGGTCCACGC 3061 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 3121 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 3181 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 3241 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 3301 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 3361 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 3421 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 3481 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 3541 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 3601 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 3661 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 3721 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 3781 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 3841 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 3901 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 3961 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 4021 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 4081 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 4141 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 4201 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 4261 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 4321 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 4381 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 4441 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 4501 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 4561 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 4621 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 4681 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 4741 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 4801 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 4861 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 4921 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 4981 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 5041 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 5101 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 5161 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 5221 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 5281 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 5341 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 5401 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 5461 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 5521 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 5581 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 5641 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 5701 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 5761 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 5821 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 5881 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 5941 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 6001 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 6061 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 6121 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 6181 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 6241 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 6301 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 6361 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 6421 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 6481 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 6541 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 6601 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 6661 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 6721 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 6781 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 6841 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 6901 GCGAATTTT //