LOCUS       RC219851                8949 bp    DNA     circular  SYN 
DEFINITION  KDR (Myc-DDK-tagged)-Human kinase insert domain receptor (a type III receptor tyrosine kinase) (KDR)
ACCESSION   NM_002253
VERSION     NM_002253.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5106..5197 
                     /label="Myc-DDK"  
     misc_feature    5227..5812 
                     /label="PolyA signal"  
     misc_feature    5284..5303 
                     /label="Restriction Enzyme"  
     misc_feature    6088..6760 
                     /label="ColE1"  
     misc_feature    7190..7984 
                     /label="Kan/Neo"  
     misc_feature    8019..8321 
                     /label="SV40 ori"  
     misc_feature    8407..8884 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5088..5094 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5088 
                     /label="KDR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAGCAAG GTGCTGCTGG CCGTCGCCCT GTGGCTCTGC GTGGAGACCC 
     1081 GGGCCGCCTC TGTGGGTTTG CCTAGTGTTT CTCTTGATCT GCCCAGGCTC AGCATACAAA 
     1141 AAGACATACT TACAATTAAG GCTAATACAA CTCTTCAAAT TACTTGCAGG GGACAGAGGG 
     1201 ACTTGGACTG GCTTTGGCCC AATAATCAGA GTGGCAGTGA GCAAAGGGTG GAGGTGACTG 
     1261 AGTGCAGCGA TGGCCTCTTC TGTAAGACAC TCACAATTCC AAAAGTGATC GGAAATGACA 
     1321 CTGGAGCCTA CAAGTGCTTC TACCGGGAAA CTGACTTGGC CTCGGTCATT TATGTCTATG 
     1381 TTCAAGATTA CAGATCTCCA TTTATTGCTT CTGTTAGTGA CCAACATGGA GTCGTGTACA 
     1441 TTACTGAGAA CAAAAACAAA ACTGTGGTGA TTCCATGTCT CGGGTCCATT TCAAATCTCA 
     1501 ACGTGTCACT TTGTGCAAGA TACCCAGAAA AGAGATTTGT TCCTGATGGT AACAGAATTT 
     1561 CCTGGGACAG CAAGAAGGGC TTTACTATTC CCAGCTACAT GATCAGCTAT GCTGGCATGG 
     1621 TCTTCTGTGA AGCAAAAATT AATGATGAAA GTTACCAGTC TATTATGTAC ATAGTTGTCG 
     1681 TTGTAGGGTA TAGGATTTAT GATGTGGTTC TGAGTCCGTC TCATGGAATT GAACTATCTG 
     1741 TTGGAGAAAA GCTTGTCTTA AATTGTACAG CAAGAACTGA ACTAAATGTG GGGATTGACT 
     1801 TCAACTGGGA ATACCCTTCT TCGAAGCATC AGCATAAGAA ACTTGTAAAC CGAGACCTAA 
     1861 AAACCCAGTC TGGGAGTGAG ATGAAGAAAT TTTTGAGCAC CTTAACTATA GATGGTATAA 
     1921 CCCGGAGTGA CCAAGGATTG TACACCTGTG CAGCATCCAG TGGGCTGATG ACCAAGAAGA 
     1981 ACAGCACATT TGTCAGGGTC CATGAAAAAC CTTTTGTTGC TTTTGGAAGT GGCATGGAAT 
     2041 CTCTGGTGGA AGCCACGGTG GGGGAGCGTG TCAGAATCCC TGCGAAGTAC CTTGGTTACC 
     2101 CACCCCCAGA AATAAAATGG TATAAAAATG GAATACCCCT TGAGTCCAAT CACACAATTA 
     2161 AAGCGGGGCA TGTACTGACG ATTATGGAAG TGAGTGAAAG AGACACAGGA AATTACACTG 
     2221 TCATCCTTAC CAATCCCATT TCAAAGGAGA AGCAGAGCCA TGTGGTCTCT CTGGTTGTGT 
     2281 ATGTCCCACC CCAGATTGGT GAGAAATCTC TAATCTCTCC TGTGGATTCC TACCAGTACG 
     2341 GCACCACTCA AACGCTGACA TGTACGGTCT ATGCCATTCC TCCCCCGCAT CACATCCACT 
     2401 GGTATTGGCA GTTGGAGGAA GAGTGCGCCA ACGAGCCCAG CCAAGCTGTC TCAGTGACAA 
     2461 ACCCATACCC TTGTGAAGAA TGGAGAAGTG TGGAGGACTT CCAGGGAGGA AATAAAATTG 
     2521 AAGTTAATAA AAATCAATTT GCTCTAATTG AAGGAAAAAA CAAAACTGTA AGTACCCTTG 
     2581 TTATCCAAGC GGCAAATGTG TCAGCTTTGT ACAAATGTGA AGCGGTCAAC AAAGTCGGGA 
     2641 GAGGAGAGAG GGTGATCTCC TTCCACGTGA CCAGGGGTCC TGAAATTACT TTGCAACCTG 
     2701 ACATGCAGCC CACTGAGCAG GAGAGCGTGT CTTTGTGGTG CACTGCAGAC AGATCTACGT 
     2761 TTGAGAACCT CACATGGTAC AAGCTTGGCC CACAGCCTCT GCCAATCCAT GTGGGAGAGT 
     2821 TGCCCACACC TGTTTGCAAG AACTTGGATA CTCTTTGGAA ATTGAATGCC ACCATGTTCT 
     2881 CTAATAGCAC AAATGACATT TTGATCATGG AGCTTAAGAA TGCATCCTTG CAGGACCAAG 
     2941 GAGACTATGT CTGCCTTGCT CAAGACAGGA AGACCAAGAA AAGACATTGC GTGGTCAGGC 
     3001 AGCTCACAGT CCTAGAGCGT GTGGCACCCA CGATCACAGG AAACCTGGAG AATCAGACGA 
     3061 CAAGTATTGG GGAAAGCATC GAAGTCTCAT GCACGGCATC TGGGAATCCC CCTCCACAGA 
     3121 TCATGTGGTT TAAAGATAAT GAGACCCTTG TAGAAGACTC AGGCATTGTA TTGAAGGATG 
     3181 GGAACCGGAA CCTCACTATC CGCAGAGTGA GGAAGGAGGA CGAAGGCCTC TACACCTGCC 
     3241 AGGCATGCAG TGTTCTTGGC TGTGCAAAAG TGGAGGCATT TTTCATAATA GAAGGTGCCC 
     3301 AGGAAAAGAC GAACTTGGAA ATCATTATTC TAGTAGGCAC GGCGGTGATT GCCATGTTCT 
     3361 TCTGGCTACT TCTTGTCATC ATCCTACGGA CCGTTAAGCG GGCCAATGGA GGGGAACTGA 
     3421 AGACAGGCTA CTTGTCCATC GTCATGGATC CAGATGAACT CCCATTGGAT GAACATTGTG 
     3481 AACGACTGCC TTATGATGCC AGCAAATGGG AATTCCCCAG AGACCGGCTG AAGCTAGGTA 
     3541 AGCCTCTTGG CCGTGGTGCC TTTGGCCAAG TGATTGAAGC AGATGCCTTT GGAATTGACA 
     3601 AGACAGCAAC TTGCAGGACA GTAGCAGTCA AAATGTTGAA AGAAGGAGCA ACACACAGTG 
     3661 AGCATCGAGC TCTCATGTCT GAACTCAAGA TCCTCATTCA TATTGGTCAC CATCTCAATG 
     3721 TGGTCAACCT TCTAGGTGCC TGTACCAAGC CAGGAGGGCC ACTCATGGTG ATTGTGGAAT 
     3781 TCTGCAAATT TGGAAACCTG TCCACTTACC TGAGGAGCAA GAGAAATGAA TTTGTCCCCT 
     3841 ACAAGACCAA AGGGGCACGA TTCCGTCAAG GGAAAGACTA CGTTGGAGCA ATCCCTGTGG 
     3901 ATCTGAAACG GCGCTTGGAC AGCATCACCA GTAGCCAGAG CTCAGCCAGC TCTGGATTTG 
     3961 TGGAGGAGAA GTCCCTCAGT GATGTAGAAG AAGAGGAAGC TCCTGAAGAT CTGTATAAGG 
     4021 ACTTCCTGAC CTTGGAGCAT CTCATCTGTT ACAGCTTCCA AGTGGCTAAG GGCATGGAGT 
     4081 TCTTGGCATC GCGAAAGTGT ATCCACAGGG ACCTGGCGGC ACGAAATATC CTCTTATCGG 
     4141 AGAAGAACGT GGTTAAAATC TGTGACTTTG GCTTGGCCCG GGATATTTAT AAAGATCCAG 
     4201 ATTATGTCAG AAAAGGAGAT GCTCGCCTCC CTTTGAAATG GATGGCCCCA GAAACAATTT 
     4261 TTGACAGAGT GTACACAATC CAGAGTGACG TCTGGTCTTT TGGTGTTTTG CTGTGGGAAA 
     4321 TATTTTCCTT AGGTGCTTCT CCATATCCTG GGGTAAAGAT TGATGAAGAA TTTTGTAGGC 
     4381 GATTGAAAGA AGGAACTAGA ATGAGGGCCC CTGATTATAC TACACCAGAA ATGTACCAGA 
     4441 CCATGCTGGA CTGCTGGCAC GGGGAGCCCA GTCAGAGACC CACGTTTTCA GAGTTGGTGG 
     4501 AACATTTGGG AAATCTCTTG CAAGCTAATG CTCAGCAGGA TGGCAAAGAC TACATTGTTC 
     4561 TTCCGATATC AGAGACTTTG AGCATGGAAG AGGATTCTGG ACTCTCTCTG CCTACCTCAC 
     4621 CTGTTTCCTG TATGGAGGAG GAGGAAGTAT GTGACCCCAA ATTCCATTAT GACAACACAG 
     4681 CAGGAATCAG TCAGTATCTG CAGAACAGTA AGCGAAAGAG CCGGCCTGTG AGTGTAAAAA 
     4741 CATTTGAAGA TATCCCGTTA GAAGAACCAG AAGTAAAAGT AATCCCAGAT GACAACCAGA 
     4801 CGGACAGTGG TATGGTTCTT GCCTCAGAAG AGCTGAAAAC TTTGGAAGAC AGAACCAAAT 
     4861 TATCTCCATC TTTTGGTGGA ATGGTGCCCA GCAAAAGCAG GGAGTCTGTG GCATCTGAAG 
     4921 GCTCAAACCA GACAAGCGGC TACCAGTCCG GATATCACTC CGATGACACA GACACCACCG 
     4981 TGTACTCCAG TGAGGAAGCA GAACTTTTAA AGCTGATAGA GATTGGAGTG CAAACCGGTA 
     5041 GCACAGCCCA GATTCTCCAG CCTGACTCGG GGACCACACT GAGCTCTCCT CCTGTTACGC 
     5101 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5161 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5221 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5281 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5341 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5401 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5461 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5521 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5581 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5641 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5701 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5761 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5821 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5881 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5941 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6001 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6061 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6121 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6181 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6241 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6301 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6361 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6421 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6481 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6541 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6601 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6661 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6721 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6781 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6841 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6901 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6961 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7021 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7081 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7141 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7201 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7261 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7321 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7381 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7441 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7501 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7561 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7621 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7681 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7741 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7801 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7861 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7921 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7981 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8041 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8101 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8161 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8281 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8341 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8401 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8461 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8521 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8581 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8641 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8701 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8761 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8821 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8881 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8941 GCGAATTTT
//