LOCUS       RC219763                7413 bp    DNA     circular  SYN 
DEFINITION  PRICKLE2 (Myc-DDK-tagged)-Human prickle homolog 2 (Drosophila) (PRICKLE2)
ACCESSION   NM_198859
VERSION     NM_198859.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3570..3661 
                     /label="Myc-DDK"  
     misc_feature    3691..4276 
                     /label="PolyA signal"  
     misc_feature    3748..3767 
                     /label="Restriction Enzyme"  
     misc_feature    4552..5224 
                     /label="ColE1"  
     misc_feature    5654..6448 
                     /label="Kan/Neo"  
     misc_feature    6483..6785 
                     /label="SV40 ori"  
     misc_feature    6871..7348 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3552..3558 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3552 
                     /label="PRICKLE2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGACAGTG ATGCCGCTGG AGATGGAGAA GACCATCAGC AAACTCATGT 
     1081 TTGACTTTCA GAGGAACTCG ACCTCAGATG ATGACTCAGG CTGTGCTTTG GAAGAGTATG 
     1141 CCTGGGTCCC GCCGGGTCTG AAGCCTGAAC AGGTACACCA GTACTATAGC TGTCTCCCAG 
     1201 AAGAGAAAGT CCCTTATGTC AACAGTCCTG GAGAGAAACT GCGAATCAAG CAGCTACTAC 
     1261 ACCAGCTGCC GCCACATGAC AATGAGGTTC GATATTGCAA CTCCCTGGAT GAGGAAGAGA 
     1321 AGAGGGAGCT GAAGCTTTTC AGCAGCCAGA GGAAACGCGA AAACTTGGGC CGCGGGAATG 
     1381 TCAGGCCTTT CCCAGTCACC ATGACAGGAG CTATTTGTGA ACAGTGCGGA GGCCAGATCA 
     1441 ATGGTGGAGA CATCGCTGTG TTTGCGTCAC GCGCTGGCCA CGGCGTTTGC TGGCACCCGC 
     1501 CGTGCTTCGT ATGCACTGTC TGCAATGAGC TCCTGGTGGA TCTGATCTAC TTTTACCAAG 
     1561 ATGGGAAGAT ATACTGTGGC AGGCACCATG CTGAGTGCCT GAAGCCGCGC TGTGCTGCCT 
     1621 GCGATGAGAT CATCTTTGCA GATGAATGCA CAGAAGCTGA GGGGCGACAC TGGCACATGA 
     1681 AACACTTTTG CTGCTTCGAG TGTGAGACAG TGCTGGGCGG CCAGCGCTAC ATCATGAAGG 
     1741 AGGGAAGACC CTACTGTTGC CACTGCTTCG AGTCCTTGTA TGCAGAATAT TGTGACACCT 
     1801 GTGCCCAACA TATAGGTATC GACCAAGGTC AAATGACCTA TGACGGCCAA CACTGGCATG 
     1861 CCACTGAGAC CTGTTTCTGC TGTGCTCACT GCAAGAAATC CCTCCTGGGG CGGCCATTCC 
     1921 TCCCGAAGCA GGGCCAGATA TTCTGCTCAC GGGCCTGCAG TGCTGGGGAA GACCCCAATG 
     1981 GTTCTGACTC CTCTGATTCC GCCTTCCAGA ACGCCAGGGC CAAGGAGTCC CGGCGCAGTG 
     2041 CCAAAATTGG CAAGAACAAG GGCAAGACGG AGGAGCCCAT GCTGAACCAG CACAGCCAGC 
     2101 TGCAAGTGAG TTCTAACCGG CTGTCAGCCG ACGTAGACCC CCTGTCACTG CAGATGGACA 
     2161 TGCTCAGCCT GTCCAGCCAG ACACCCAGCC TCAACCGGGA CCCCATCTGG AGGAGCCGGG 
     2221 AAGAGCCCTA CCATTATGGG AACAAGATGG AGCAGAACCA GACCCAGAGC CCTCTGCAGC 
     2281 TCCTCAGCCA GTGCAACATC AGAACTTCCT ACAGTCCAGG AGGGCAAGGG GCTGGGGCCC 
     2341 AGCCCGAAAT GTGGGGCAAG CACTTCAGCA ACCCCAAAAG GAGCTCGTCA CTGGCCATGA 
     2401 CAGGACATGC TGGCAGCTTC ATCAAGGAAT GCCGAGAAGA CTATTACCCG GGGAGGCTGA 
     2461 GATCTCAGGA GAGCTACAGT GATATGTCTA GTCAGAGTTT CAGTGAGACC CGAGGCAGCA 
     2521 TCCAAGTCCC CAAATATGAG GAGGAAGAGG AAGAGGAAGG GGGCTTGTCC ACTCAGCAGT 
     2581 GTCGGACCCG TCATCCCATC AGTTCCCTGA AATACACAGA GGACATGACG CCCACAGAGC 
     2641 AGACCCCTCG GGGCTCCATG GAATCCCTGG CCCTGTCTAA TGCAACAGGC CTCTCTGCTG 
     2701 ATGGTGGTGC CAAGCGCCAG GAGCACCTAT CCCGATTTTC CATGCCTGAC CTCAGCAAAG 
     2761 ACTCTGGAAT GAATGTGTCT GAGAAGCTGA GCAACATGGG CACTCTTAAC TCGTCCATGC 
     2821 AGTTCCGGAG CGCAGAGTCA GTTCGCAGCC TGCTCTCTGC CCAGCAGTAC CAGGAGATGG 
     2881 AGGGAAACCT CCACCAGCTC AGCAACCCCA TTGGCTACAG AGACCTGCAG TCCCACGGAA 
     2941 GGATGCATCA GAGCTTTGAT TTTGATGGAG GGATGGCGGG CAGCAAGCTG CCAGGGCAGG 
     3001 AGGGCGTGAG GATCCAGCCC ATGAGTGAAC GCACCCGGAG AAGAGCTACT TCACGCGACG 
     3061 ACAACCGCCG TTTCCGACCT CACAGGTCCA GGCGTTCCCG ACGCTCTCGC TCCGACAACG 
     3121 CCCTCCACCT GGCCAGCGAA CGCGAGGCCA TCTCCCGGTT AAAAGATAGG CCCCCTCTGA 
     3181 GAGCCAGGGA GGACTATGAC CAATTTATGC GCCAGCGGAG CTTCCAGGAG AGCATGGGGC 
     3241 ATGGGTCCCG GAGGGACCTG TACGGCCAGT GCCCTAGGAC TGTGTCGGAC CTGGCTTTGC 
     3301 AGAATGCCTT TGGGGACCGC TGGGGACCCT ACTTCGCCGA GTATGATTGG TGTTCCACCT 
     3361 GCTCCTCCTC TTCAGAGTCT GACAACGAGG GCTATTTCCT AGGAGAACCC ATCCCCCAGC 
     3421 CAGCGCGCCT GCGATACGTC ACAAGCGATG AGCTGCTGCA CAAATACAGC TCCTACGGCC 
     3481 TCCCCAAATC TTCCACATTA GGTGGCAGAG GACAGTTGCA CAGCAGGAAA AGACAGAAGA 
     3541 GCAAAAACTG TATCATTTCT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3601 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3661 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3721 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3781 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3841 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3901 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3961 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4021 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4081 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4141 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4201 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4261 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4321 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4381 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4441 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4501 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4561 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4621 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4681 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4741 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4801 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4861 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4921 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4981 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5041 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5101 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5161 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5221 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5281 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5341 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5401 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5461 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5521 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5581 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5641 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5701 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5761 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5821 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5881 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5941 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6001 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6061 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6121 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6181 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6241 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6301 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6361 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6421 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6481 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6541 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6601 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6661 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6721 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6781 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6841 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6901 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6961 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7021 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7081 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7141 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7201 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7261 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7321 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7381 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//