LOCUS       RC219645                7716 bp    DNA     circular  SYN 
DEFINITION  DGKZ (Myc-DDK-tagged)-Human diacylglycerol kinase, zeta (DGKZ), transcript variant 1
ACCESSION   NM_201532
VERSION     NM_201532.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3873..3964 
                     /label="Myc-DDK"  
     misc_feature    3994..4579 
                     /label="PolyA signal"  
     misc_feature    4051..4070 
                     /label="Restriction Enzyme"  
     misc_feature    4855..5527 
                     /label="ColE1"  
     misc_feature    5957..6751 
                     /label="Kan/Neo"  
     misc_feature    6786..7088 
                     /label="SV40 ori"  
     misc_feature    7174..7651 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3855..3861 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3855 
                     /label="DGKZ"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGAGGGG CAGGGCGGCG GAGGGCAGCG CTGGGACTGG GCTGGCGGCG 
     1081 GCCGGGCAGC CGAGGAGGAG GTGGTGCGGC GGCGATGCCG GCGCGGGGAG GAGGCCCAGG 
     1141 TCGCGCAGCC CTGGCCCGAG GGTTCCCGGG GCACGGCCGC TGGGCCCCCG GTGGAGGAGC 
     1201 GTTTCCGCCA GCTGCACCTA CGAAAGCAGG TGTCTTACAG GAAAGCCATC ACCAAGTCGG 
     1261 GCCTCCAGCA CCTGGCCCCC CCTCCGCCCA CCCCTGGGGC CCCGTGCAGC GAGTCAGAGC 
     1321 GGCAGATCCG GAGTACAGTG GACTGGAGCG AGTCAGCGAC ATATGGGGAG CACATCTGGT 
     1381 TCGAGACCAA CGTGTCCGGG GACTTCTGCT ACGTTGGGGA GCAGTACTGT GTAGCCAGGA 
     1441 TGCTGAAGTC AGTGTCTCGA AGAAAGTGCG CAGCCTGCAA GATTGTGGTG CACACGCCCT 
     1501 GCATCGAGCA GCTGGAGAAG ATAAATTTCC GCTGTAAGCC GTCCTTCCGT GAATCAGGCT 
     1561 CCAGGAATGT CCGCGAGCCA ACCTTTGTAC GGCACCACTG GGTACACAGA CGACGCCAGG 
     1621 ACGGCAAGTG TCGGCACTGT GGGAAGGGAT TCCAGCAGAA GTTCACCTTC CACAGCAAGG 
     1681 AGATTGTGGC CATCAGCTGC TCGTGGTGCA AGCAGGCATA CCACAGCAAG GTGTCCTGCT 
     1741 TCATGCTGCA GCAGATCGAG GAGCCGTGCT CGCTGGGGGT CCACGCAGCC GTGGTCATCC 
     1801 CGCCCACCTG GATCCTCCGC GCCCGGAGGC CCCAGAATAC TCTGAAAGCA AGCAAGAAGA 
     1861 AGAAGAGGGC ATCCTTCAAG AGGAAGTCCA GCAAGAAAGG GCCTGAGGAG GGCCGCTGGA 
     1921 GACCCTTCAT CATCAGGCCC ACCCCCTCCC CGCTCATGAA GCCCCTGCTG GTGTTTGTGA 
     1981 ACCCCAAGAG TGGGGGCAAC CAGGGTGCAA AGATCATCCA GTCTTTCCTC TGGTATCTCA 
     2041 ATCCCCGACA AGTCTTCGAC CTGAGCCAGG GAGGGCCCAA GGAGGCGCTG GAGATGTACC 
     2101 GCAAAGTGCA CAACCTGCGG ATCCTGGCGT GCGGGGGCGA CGGCACGGTG GGCTGGATCC 
     2161 TCTCCACCCT GGACCAGCTA CGCCTGAAGC CGCCACCCCC TGTTGCCATC CTGCCCCTGG 
     2221 GTACTGGCAA CGACTTGGCC CGAACCCTCA ACTGGGGTGG GGGCTACACA GATGAGCCTG 
     2281 TGTCCAAGAT CCTCTCCCAC GTGGAGGAGG GGAACGTGGT ACAGCTGGAC CGCTGGGACC 
     2341 TCCACGCTGA GCCCAACCCC GAGGCAGGGC CTGAGGACCG AGATGAAGGC GCCACCGACC 
     2401 GGTTGCCCCT GGATGTCTTC AACAACTACT TCAGCCTGGG CTTTGACGCC CACGTCACCC 
     2461 TGGAGTTCCA CGAGTCTCGA GAGGCCAACC CAGAGAAATT CAACAGCCGC TTTCGGAATA 
     2521 AGATGTTCTA CGCCGGGACA GCTTTCTCTG ACTTCCTGAT GGGCAGCTCC AAGGACCTGG 
     2581 CCAAGCACAT CCGAGTGGTG TGTGATGGAA TGGACTTGAC TCCCAAGATC CAGGACCTGA 
     2641 AACCCCAGTG TGTTGTTTTC CTGAACATCC CCAGGTACTG TGCGGGCACC ATGCCCTGGG 
     2701 GCCACCCTGG GGAGCACCAC GACTTTGAGC CCCAGCGGCA TGACGACGGC TACCTCGAGG 
     2761 TCATTGGCTT CACCATGACG TCGTTGGCCG CGCTGCAGGT GGGCGGACAC GGCGAGCGGC 
     2821 TGACGCAGTG TCGCGAGGTG GTGCTCACCA CATCCAAGGC CATCCCGGTG CAGGTGGATG 
     2881 GCGAGCCCTG CAAGCTTGCA GCCTCACGCA TCCGCATCGC CCTGCGCAAC CAGGCCACCA 
     2941 TGGTGCAGAA GGCCAAGCGG CGGAGCGCCG CCCCCCTGCA CAGCGACCAG CAGCCGGTGC 
     3001 CAGAGCAGTT GCGCATCCAG GTGAGTCGCG TCAGCATGCA CGACTATGAG GCCCTGCACT 
     3061 ACGACAAGGA GCAGCTCAAG GAGGCCTCTG TGCCGCTGGG CACTGTGGTG GTCCCAGGAG 
     3121 ACAGTGACCT AGAGCTCTGC CGTGCCCACA TTGAGAGACT CCAGCAGGAG CCCGATGGTG 
     3181 CTGGAGCCAA GTCCCCGACA TGCCAGAAAC TGTCCCCCAA GTGGTGCTTC CTGGACGCCA 
     3241 CCACTGCCAG CCGCTTCTAC AGGATCGACC GAGCCCAGGA GCACCTCAAC TATGTGACTG 
     3301 AGATCGCACA GGATGAGATT TATATCCTGG ACCCTGAGCT GCTGGGGGCA TCGGCCCGGC 
     3361 CTGACCTCCC AACCCCCACT TCCCCTCTCC CCACCTCACC CTGCTCACCC ACGCCCCGGT 
     3421 CACTGCAAGG GGATGCTGCA CCCCCTCAAG GTGAAGAGCT GATTGAGGCT GCCAAGAGGA 
     3481 ACGACTTCTG TAAGCTCCAG GAGCTGCACC GAGCTGGGGG CGACCTCATG CACCGAGACG 
     3541 AGCAGAGTCG CACGCTCCTG CACCACGCAG TCAGCACTGG CAGCAAGGAT GTGGTCCGCT 
     3601 ACCTGCTGGA CCACGCCCCC CCAGAGATCC TTGATGCGGT GGAGGAAAAC GGGGAGACCT 
     3661 GTTTGCACCA AGCAGCGGCC CTGGGCCAGC GCACCATCTG CCACTACATC GTGGAGGCCG 
     3721 GGGCCTCGCT CATGAAGACA GACCAGCAGG GCGACACTCC CCGGCAGCGG GCTGAGAAGG 
     3781 CTCAGGACAC CGAGCTGGCC GCCTACCTGG AGAACCGGCA GCACTACCAG ATGATCCAGC 
     3841 GGGAGGACCA GGAGACGGCT GTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3901 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3961 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4021 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4081 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4141 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4201 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4261 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4321 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4381 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4441 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4501 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4561 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4621 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4681 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4741 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4801 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4861 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4921 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4981 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5041 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5101 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5161 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5221 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5281 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5341 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5401 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5461 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5521 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5581 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5641 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5701 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5761 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5821 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5881 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5941 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6001 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6061 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6121 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6181 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6241 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6301 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6361 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6421 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6481 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6541 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6601 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6661 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6721 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6781 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6841 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6901 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6961 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7081 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7141 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7201 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7261 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7321 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7381 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7441 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7501 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7561 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7621 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7681 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//