LOCUS       RC219605                8793 bp    DNA     circular  SYN 
DEFINITION  SF3B1 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 1, 155kDa (SF3B1), transcript variant 1. Note: ORF is codon optimized
ACCESSION   NM_012433
VERSION     NM_012433.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4950..5041 
                     /label="Myc-DDK"  
     misc_feature    5071..5656 
                     /label="PolyA signal"  
     misc_feature    5128..5147 
                     /label="Restriction Enzyme"  
     misc_feature    5932..6604 
                     /label="ColE1"  
     misc_feature    7034..7828 
                     /label="Kan/Neo"  
     misc_feature    7863..8165 
                     /label="SV40 ori"  
     misc_feature    8251..8728 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4932..4938 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4932 
                     /label="SF3B1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAAGATC GCCAAGACTC ACGAAGATAT TGAAGCACAG ATTCGAGAAA 
     1081 TTCAAGGCAA GAAGGCAGCT CTTGATGAAG CTCAAGGAGT GGGCCTCGAT TCTACAGGTT 
     1141 ATTATGACCA GGAAATTTAT GGTGGAAGTG ACAGCAGATT TGCTGGATAC GTGACATCAA 
     1201 TTGCTGCAAC TGAACTTGAA GATGATGACG ATGACTATTC ATCATCTACG AGTTTGCTTG 
     1261 GTCAGAAGAA GCCAGGATAT CATGCCCCTG TGGCATTGCT TAATGATATA CCACAGTCAA 
     1321 CAGAACAGTA TGATCCATTT GCTGAGCACA GACCTCCAAA GATTGCAGAC CGGGAAGATG 
     1381 AATACAAAAA GCATAGGCGG ACCATGATAA TTTCCCCAGA GCGTCTTGAT CCTTTTGCAG 
     1441 ATGGAGGGAA AACCCCTGAT CCTAAAATGA ATGCTAGGAC TTACATGGAT GTAATGCGAG 
     1501 AACAACACTT GACTAAAGAA GAACGAGAAA TTAGGCAACA GCTAGCAGAA AAAGCTAAAG 
     1561 CTGGAGAACT AAAAGTCGTC AATGGAGCAG CAGCGTCCCA GCCTCCATCA AAACGAAAAC 
     1621 GGCGTTGGGA TCAAACAGCT GATCAGACTC CTGGTGCCAC TCCCAAAAAA CTATCAAGTT 
     1681 GGGATCAGGC AGAGACCCCT GGGCATACTC CTTCCTTAAG ATGGGATGAG ACACCAGGTC 
     1741 GTGCAAAGGG AAGCGAGACT CCTGGAGCAA CCCCAGGCTC AAAAATATGG GATCCTACAC 
     1801 CTAGCCACAC ACCAGCGGGA GCTGCTACTC CTGGACGAGG TGATACACCA GGCCATGCGA 
     1861 CACCAGGCCA TGGAGGCGCA ACTTCCAGTG CTCGTAAAAA CAGATGGGAT GAAACCCCCA 
     1921 AAACAGAGAG AGATACTCCT GGGCATGGAA GTGGATGGGC TGAGACTCCT CGAACAGATC 
     1981 GAGGTGGAGA TTCTATTGGT GAAACACCGA CTCCTGGAGC CAGTAAAAGA AAATCACGGT 
     2041 GGGATGAAAC ACCAGCTAGT CAGATGGGTG GAAGCACTCC AGTTCTGACC CCTGGAAAGA 
     2101 CACCAATTGG CACACCAGCC ATGAACATGG CTACCCCTAC TCCAGGTCAC ATAATGAGTA 
     2161 TGACTCCTGA ACAGCTTCAG GCTTGGCGGT GGGAAAGAGA AATTGATGAG AGAAATCGCC 
     2221 CACTTTCTGA TGAGGAATTA GATGCTATGT TCCCAGAAGG ATATAAGGTA CTTCCTCCTC 
     2281 CAGCTGGTTA TGTTCCTATT CGAACTCCAG CTCGAAAGCT GACAGCTACT CCAACACCTT 
     2341 TGGGTGGTAT GACTGGTTTC CACATGCAAA CTGAAGATCG AACTATGAAA AGTGTTAATG 
     2401 ACCAGCCATC TGGAAATCTT CCATTTTTAA AACCTGATGA TATTCAATAC TTTGATAAAC 
     2461 TATTGGTTGA TGTTGATGAA TCAACACTTA GTCCAGAAGA GCAAAAAGAG AGAAAAATAA 
     2521 TGAAGTTGCT TTTAAAAATT AAGAATGGAA CACCACCAAT GAGAAAGGCT GCATTGCGTC 
     2581 AGATTACTGA TAAAGCTCGT GAATTTGGAG CTGGTCCTTT GTTTAATCAG ATTCTTCCTC 
     2641 TGCTGATGTC TCCTACACTT GAGGATCAAG AGCGTCATTT ACTTGTGAAA GTTATTGATA 
     2701 GGATACTGTA CAAACTTGAT GACTTAGTTC GTCCATATGT GCATAAGATC CTCGTGGTCA 
     2761 TTGAACCGCT ATTGATTGAT GAAGATTACT ATGCTAGAGT GGAAGGCCGA GAGATCATTT 
     2821 CTAATTTGGC AAAGGCTGCT GGTCTGGCTA CTATGATCTC TACCATGAGA CCTGATATAG 
     2881 ATAACATGGA TGAGTATGTC CGTAACACAA CAGCTAGAGC TTTTGCTGTT GTAGCCTCTG 
     2941 CCCTGGGCAT TCCTTCTTTA TTGCCCTTCT TAAAAGCTGT GTGCAAAAGC AAGAAGTCCT 
     3001 GGCAAGCGAG ACACACTGGT ATTAAGATTG TACAACAGAT AGCTATTCTT ATGGGCTGTG 
     3061 CCATCTTGCC ACATCTTAGA AGTTTAGTTG AAATCATTGA ACATGGTCTT GTGGATGAGC 
     3121 AGCAGAAAGT TCGGACCATC AGTGCTTTGG CCATTGCTGC CTTGGCTGAA GCAGCAACTC 
     3181 CTTATGGTAT CGAATCTTTT GATTCTGTGT TAAAGCCTTT ATGGAAGGGT ATCCGCCAAC 
     3241 ACAGAGGAAA GGGTTTGGCT GCTTTCTTGA AGGCTATTGG GTATCTTATT CCTCTTATGG 
     3301 ATGCAGAATA TGCCAACTAC TATACTAGAG AAGTGATGTT AATCCTTATT CGAGAATTCC 
     3361 AGTCTCCTGA TGAGGAAATG AAAAAAATTG TGCTGAAGGT GGTAAAACAG TGTTGTGGGA 
     3421 CAGATGGTGT AGAAGCAAAC TACATTAAAA CAGAGATTCT TCCTCCCTTT TTTAAACACT 
     3481 TCTGGCAGCA CAGGATGGCT TTGGATAGAA GAAATTACCG ACAGTTAGTT GATACTACTG 
     3541 TGGAGTTGGC AAACAAAGTA GGTGCAGCAG AAATTATATC CAGGATTGTG GATGATCTGA 
     3601 AAGATGAAGC CGAACAGTAC AGAAAAATGG TGATGGAGAC AATTGAGAAG ATTATGGGGA 
     3661 ACCTCGGGGC GGCAGACATT GATCATAAGC TCGAAGAACA GCTGATCGAC GGGATCCTGT 
     3721 ATGCATTTCA GGAGCAGACC ACGGAAGACT CAGTGATGCT TAACGGCTTT GGCACTGTCG 
     3781 TAAACGCCCT TGGCAAACGG GTCAAACCCT ATCTTCCGCA GATCTGTGGC ACTGTGCTGT 
     3841 GGAGGTTGAA TAACAAATCT GCCAAAGTTA GACAGCAGGC AGCCGATCTC ATTTCTCGGA 
     3901 CCGCAGTGGT TATGAAGACA TGTCAGGAGG AAAAGTTGAT GGGACATTTG GGTGTAGTGC 
     3961 TCTACGAATA CTTGGGGGAA GAATATCCCG AAGTGCTCGG CTCCATCCTC GGCGCGCTGA 
     4021 AGGCAATCGT AAATGTTATT GGGATGCATA AGATGACGCC ACCTATCAAG GACCTCTTGC 
     4081 CGCGCCTGAC CCCGATCCTC AAGAATAGGC ATGAGAAGGT ACAGGAAAAC TGTATCGACC 
     4141 TGGTGGGAAG AATCGCTGAT CGGGGAGCCG AATACGTCTC AGCCCGGGAG TGGATGAGAA 
     4201 TTTGCTTCGA ACTGCTGGAG CTGCTGAAGG CCCATAAGAA GGCAATTCGG CGCGCCACGG 
     4261 TTAATACCTT TGGCTACATA GCTAAGGCCA TCGGGCCTCA CGACGTCCTC GCAACCCTGC 
     4321 TGAACAATCT GAAAGTTCAG GAACGGCAGA ACAGGGTGTG TACGACCGTG GCCATTGCCA 
     4381 TTGTGGCTGA AACGTGTAGC CCCTTCACCG TCCTGCCCGC CCTCATGAAC GAGTACCGCG 
     4441 TCCCGGAGTT GAACGTGCAG AACGGGGTCC TCAAGAGTCT GAGCTTTTTG TTCGAGTATA 
     4501 TCGGAGAGAT GGGCAAAGAC TACATTTACG CAGTGACCCC ACTTCTGGAA GACGCCCTGA 
     4561 TGGACCGGGA TCTGGTCCAC AGGCAGACCG CTTCCGCTGT TGTCCAGCAT ATGAGTCTCG 
     4621 GAGTTTATGG ATTCGGCTGT GAGGACAGTC TGAATCATTT GCTTAACTAT GTATGGCCCA 
     4681 ACGTGTTTGA AACTTCCCCT CATGTTATTC AGGCCGTGAT GGGAGCTCTT GAGGGGCTCC 
     4741 GGGTAGCAAT CGGCCCCTGC CGGATGCTGC AGTACTGCCT CCAGGGGCTC TTCCATCCTG 
     4801 CAAGGAAAGT GAGAGATGTT TATTGGAAGA TCTACAACAG TATTTACATA GGGTCCCAAG 
     4861 ACGCTTTGAT TGCTCACTAC CCTCGGATCT ACAATGACGA CAAGAATACT TACATTAGGT 
     4921 ACGAACTGGA CTACATTCTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4981 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5041 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5101 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5161 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5221 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5281 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5341 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5401 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5461 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5521 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5581 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5641 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5701 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5761 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5821 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5881 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5941 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6001 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6061 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6121 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6181 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6241 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6301 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6361 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6421 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6481 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6541 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6601 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6661 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6721 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6781 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6841 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6901 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6961 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7021 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7081 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7141 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7201 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7261 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7321 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7381 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7441 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7501 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7561 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7621 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7681 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7741 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7801 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7861 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7921 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7981 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8041 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8101 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8161 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8221 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8281 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8341 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8401 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8461 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8521 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8581 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8641 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8701 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8761 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//