LOCUS       RC219542                8310 bp    DNA     circular  SYN 
DEFINITION  CACNA2D2 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 2 (CACNA2D2), transcript variant 2
ACCESSION   NM_006030
VERSION     NM_006030.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4467..4558 
                     /label="Myc-DDK"  
     misc_feature    4588..5173 
                     /label="PolyA signal"  
     misc_feature    4645..4664 
                     /label="Restriction Enzyme"  
     misc_feature    5449..6121 
                     /label="ColE1"  
     misc_feature    6551..7345 
                     /label="Kan/Neo"  
     misc_feature    7380..7682 
                     /label="SV40 ori"  
     misc_feature    7768..8245 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4449..4455 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4449 
                     /label="CACNA2D2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGCCG GCTCGGACCT GCGGCGCCTC TCGGCCCGGC CCAGCGCGGA 
     1081 CTGCGCGCCC CTGGCCCGGC TGCGGCCCCC ACCCTGGCCC CGGCACCCGG CGCCCGACGT 
     1141 CCGGGCCCCC GCGCCCGCTG TGGCTGCTGC TGCCGCTTCT ACCGCTGCTC GCCGCCCCCG 
     1201 GCGCCTCTGC CTACAGCTTC CCCCAGCAGC ACACGATGCA GCACTGGGCC CGGCGTCTGG 
     1261 AGCAGGAGGT CGACGGCGTG ATGCGGATTT TTGGAGGCGT CCAGCAGCTC CGTGAGATTT 
     1321 ACAAGGACAA CCGGAACCTG TTCGAGGTAC AGGAGAATGA GCCTCAGAAG TTGGTGGAGA 
     1381 AGGTGGCAGG GGACATTGAG AGCCTTCTGG ACAGGAAGGT GCAGGCCCTG AAGAGACTGG 
     1441 CTGATGCTGC AGAGAACTTC CAGAAAGCAC ACCGCTGGCA GGACAACATC AAGGAGGAAG 
     1501 ACATCGTGTA CTATGACGCC AAGGCTGACG CTGAGCTGGA CGACCCTGAG AGTGAGGATG 
     1561 TGGAAAGGGG GTCTAAGGCC AGCACCCTAA GGCTGGACTT CATCGAGGAC CCAAACTTCA 
     1621 AGAACAAGGT CAACTATTCA TACGCGGCTG TACAGATCCC TACGGACATC TACAAAGGCT 
     1681 CCACTGTCAT CCTCAATGAG CTCAACTGGA CAGAGGCCCT GGAGAATGTG TTCATGGAAA 
     1741 ACCGCAGACA AGACCCCACA CTGCTGTGGC AGGTCTTCGG CAGCGCCACA GGAGTCACTC 
     1801 GCTACTACCC GGCCACCCCG TGGCGAGCCC CCAAGAAGAT CGACCTGTAC GATGTCCGAA 
     1861 GGAGACCCTG GTATATCCAG GGGGCCTCGT CACCCAAAGA CATGGTCATC ATCGTGGATG 
     1921 TGAGTGGCAG TGTGAGCGGC CTGACCCTGA AGCTGATGAA GACATCTGTC TGCGAGATGC 
     1981 TGGACACGCT GTCTGATGAT GACTATGTGA ATGTGGCCTC GTTCAACGAG AAGGCACAGC 
     2041 CTGTGTCATG CTTCACACAC CTGGTGCAGG CCAATGTGCG CAACAAGAAG GTGTTCAAGG 
     2101 AAGCTGTGCA GGGCATGGTG GCCAAGGGCA CCACAGGCTA CAAGGCCGGC TTTGAGTATG 
     2161 CCTTTGACCA GCTGCAGAAC TCCAACATCA CTCGGGCCAA CTGCAACAAG ATGATCATGA 
     2221 TGTTCACGGA TGGTGGTGAG GACCGCGTGC AGGACGTCTT TGAGAAGTAC AATTGGCCAA 
     2281 ACCGGACGGT GCGCGTGTTT ACTTTCTCCG TGGGGCAGCA TAACTATGAC GTCACACCGC 
     2341 TGCAGTGGAT GGCCTGTGCC AACAAAGGCT ACTATTTTGA GATCCCTTCC ATCGGAGCCA 
     2401 TCCGCATCAA CACACAGGAA TATCTAGATG TGTTGGGCAG GCCCATGGTG CTGGCAGGCA 
     2461 AGGAGGCCAA GCAGGTGCAG TGGACCAACG TGTATGAGGA TGCACTGGGA CTGGGGTTGG 
     2521 TGGTAACAGG GACCCTCCCT GTTTTCAACC TGACACAGGA TGGCCCTGGG GAAAAGAAGA 
     2581 ACCAGCTGAT CCTGGGCGTG ATGGGCATTG ACGTGGCTCT GAATGACATC AAGAGGCTGA 
     2641 CCCCCAACTA CACGCTTGGA GCCAACGGCT ATGTGTTTGC CATTGACCTG AACGGCTACG 
     2701 TGTTGCTGCA CCCCAATCTC AAGCCCCAGA CCACCAACTT CCGGGAGCCT GTGACTCTGG 
     2761 ACTTCCTGGA TGCGGAGCTA GAGGATGAGA ACAAGGAAGA GATCCGTCGG AGCATGATTG 
     2821 ATGGCAACAA GGGCCACAAG CAGATCAGAA CGTTGGTCAA GTCCCTGGAT GAGAGGTACA 
     2881 TAGATGAGGT GACACGGAAC TACACCTGGG TGCCTATAAG GAGCACTAAC TACAGCCTGG 
     2941 GGCTGGTGCT CCCACCCTAC AGCACCTTCT ACCTCCAAGC CAATCTCAGT GACCAGATCC 
     3001 TGCAGGTCAA GTATTTTGAG TTCCTGCTCC CCAGCAGCTT TGAGTCTGAA GGACACGTTT 
     3061 TCATTGCTCC CAGAGAGTAC TGCAAGGACC TGAATGCCTC AGACAACAAC ACCGAGTTCC 
     3121 TGAAAAACTT TATTGAGCTC ATGGAGAAAG TGACTCCAGA CTCCAAGCAG TGCAACAACT 
     3181 TCCTTCTGCA CAACCTGATC TTGGACACGG GCATCACGCA GCAGCTGGTA GAGCGTGTGT 
     3241 GGAGGGACCA GGATCTCAAC ACGTACAGCC TACTGGCCGT GTTCGCTGCC ACAGACGGTG 
     3301 GCATCACCCG AGTCTTCCCC AACAAGGCAG CTGAGGACTG GACAGAGAAC CCTGAGCCCT 
     3361 TCAATGCCAG CTTCTACCGC CGCAGCCTGG ATAACCACGG TTATGTCTTC AAGCCCCCAC 
     3421 ACCAGGATGC CCTGTTAAGG CCGCTGGAGC TGGAGAATGA CACTGTGGGC ATCCTCGTCA 
     3481 GCACAGCTGT GGAGCTCAGC CTAGGCAGGC GCACACTGAG GCCAGCAGTG GTGGGCGTCA 
     3541 AGCTGGACCT AGAGGCTTGG GCTGAGAAGT TCAAGGTGCT AGCCAGCAAC CGTACCCACC 
     3601 AAGACCAGCC TCAGAAGTGC GGCCCCAACA GCCACTGTGA GATGGACTGC GAGGTTAACA 
     3661 ATGAGGACTT ACTCTGTGTC CTCATTGATG ATGGAGGATT CCTGGTGCTG TCAAACCAGA 
     3721 ACCATCAGTG GGACCAGGTG GGCAGGTTCT TCAGTGAGGT GGATGCCAAC CTGATGCTGG 
     3781 CACTCTACAA TAACTCCTTC TACACCCGCA AGGAGTCCTA TGACTATCAG GCAGCCTGTG 
     3841 CCCCTCAGCC CCCTGGCAAC CTGGGTGCTG CACCCCGGGG TGTCTTTGTG CCCACCGTTG 
     3901 CAGATTTCCT TAACCTGGCC TGGTGGACCT CTGCTGCCGC CTGGTCCCTG TTCCAGCAGC 
     3961 TTCTCTACGG CCTCATCTAC CACAGCTGGT TCCAAGCAGA CCCCGCGGAG GCCGAGGGGA 
     4021 GCCCCGAGAC GCGCGAGAGC AGCTGCGTCA TGAAACAGAC CCAGTACTAC TTCGGCTCGG 
     4081 TAAACGCCTC CTACAACGCC ATCATCGACT GCGGAAACTG CTCCAGGCTG TTCCACGCGC 
     4141 AGAGACTGAC CAACACCAAT CTTCTCTTTG TGGTGGCCGA GAAGCCGCTG TGCAGCCAGT 
     4201 GCGAGGCTGG CCGGCTGCTG CAGAAGGAGA CGCACTCGGA CGGCCCGGAG CAGTGTGAGC 
     4261 TAGTGCAGAG ACCGCGATAC CGGAGAGGCC CGCACATCTG CTTCGACTAC AACGCGACAG 
     4321 AAGATACCTC AGACTGTGGC CGCGGGGCCT CCTTCCCGCC GTCGCTGGGC GTCCTGGTCT 
     4381 CCCTGCAACT GCTGCTCCTC CTGGGCCTGC CGCCCCGGCC GCAGCCTCAA GTCCTCGTCC 
     4441 ACGCCTCTCG CCGCCTCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4501 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4561 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4621 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4681 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4741 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4801 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4861 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4921 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4981 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5041 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5101 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5161 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5221 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5281 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5341 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5401 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5461 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5521 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5581 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5641 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5701 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5761 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5821 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5881 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5941 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6001 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6061 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6121 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6181 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6241 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6301 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6361 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6421 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6481 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6541 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6601 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6661 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6721 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6781 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6841 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6901 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6961 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7021 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7081 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7141 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7201 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7261 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7321 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7381 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7441 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7501 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7561 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7621 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7681 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7741 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7801 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7861 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7921 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7981 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8041 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8101 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8161 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8221 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8281 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//