LOCUS       RC219513L1                9549 bp    DNA     circular  SYN 
DEFINITION  UBA1 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 2
ACCESSION   NM_153280
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7840..8020 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8748..9407 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8179..8747 
                     /label="ColE1"  
     misc_feature    6715..6735 
                     /label="LR50 primer"  
     misc_feature    6512..6583 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6724..7315 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6494..6500 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6494 
                     /label="UBA1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCCAGCTCG CCGCTGTCCA AGAAACGTCG 
     3361 CGTGTCCGGG CCTGATCCAA AGCCGGGTTC TAACTGCTCC CCTGCCCAGT CCGTGTTGTC 
     3421 CGAAGTGCCC TCGGTGCCAA CCAACGGAAT GGCCAAGAAC GGCAGTGAAG CAGACATAGA 
     3481 CGAGGGCCTT TACTCCCGGC AGCTGTATGT GTTGGGCCAT GAGGCAATGA AGCGGCTCCA 
     3541 GACATCCAGT GTCCTGGTAT CAGGCCTGCG GGGCCTGGGC GTGGAGATCG CTAAGAACAT 
     3601 CATCCTTGGT GGGGTCAAGG CTGTTACCCT ACATGACCAG GGCACTGCCC AGTGGGCTGA 
     3661 TCTTTCCTCC CAGTTCTACC TGCGGGAGGA GGACATCGGT AAAAACCGGG CCGAGGTATC 
     3721 ACAGCCCCGC CTCGCTGAGC TCAACAGCTA TGTGCCTGTC ACTGCCTACA CTGGACCCCT 
     3781 CGTTGAGGAC TTCCTTAGTG GTTTCCAGGT GGTGGTGCTC ACCAACACCC CCCTGGAGGA 
     3841 CCAGCTGCGA GTGGGTGAGT TCTGTCACAA CCGTGGCATC AAGCTGGTGG TGGCAGACAC 
     3901 GCGGGGCCTG TTTGGGCAGC TCTTCTGTGA CTTTGGAGAG GAAATGATCC TCACAGATTC 
     3961 CAATGGGGAG CAGCCACTCA GTGCTATGGT TTCTATGGTT ACCAAGGACA ACCCCGGTGT 
     4021 GGTTACCTGC CTGGATGAGG CCCGACACGG GTTTGAGAGC GGGGACTTTG TCTCCTTTTC 
     4081 AGAAGTACAG GGCATGGTTG AACTCAACGG AAATCAGCCC ATGGAGATCA AAGTCCTGGG 
     4141 TCCTTATACC TTTAGCATCT GTGACACCTC CAACTTCTCC GACTACATCC GTGGAGGCAT 
     4201 CGTCAGTCAG GTCAAAGTAC CTAAGAAGAT TAGCTTTAAA TCCTTGGTGG CCTCACTGGC 
     4261 AGAACCTGAC TTTGTGGTGA CGGACTTCGC CAAGTTTTCT CGCCCTGCCC AGCTGCACAT 
     4321 TGGCTTCCAG GCCCTGCACC AGTTCTGTGC TCAGCATGGC CGGCCACCTC GGCCCCGCAA 
     4381 TGAGGAGGAT GCAGCAGAAC TGGTAGCCTT AGCACAGGCT GTGAATGCTC GAGCCCTGCC 
     4441 AGCAGTGCAG CAAAATAACC TGGACGAGGA CCTCATCCGG AAGCTGGCAT ATGTGGCTGC 
     4501 TGGGGATCTG GCACCCATAA ACGCCTTCAT TGGGGGCCTG GCTGCCCAGG AAGTCATGAA 
     4561 GGCCTGCTCC GGGAAGTTCA TGCCCATCAT GCAGTGGCTA TACTTTGATG CCCTTGAGTG 
     4621 TCTCCCTGAG GACAAAGAGG TCCTCACAGA GGACAAGTGC CTCCAGCGCC AGAACCGTTA 
     4681 TGACGGGCAA GTGGCTGTGT TTGGCTCAGA CCTGCAAGAG AAGCTGGGCA AGCAGAAGTA 
     4741 TTTCCTGGTG GGTGCGGGGG CCATTGGCTG TGAGCTGCTC AAGAACTTTG CCATGATTGG 
     4801 GCTGGGCTGC GGGGAGGGTG GAGAAATCAT CGTTACAGAC ATGGACACCA TTGAGAAGTC 
     4861 AAATCTGAAT CGACAGTTTC TTTTCCGGCC CTGGGATGTC ACGAAGTTAA AGTCTGACAC 
     4921 GGCTGCTGCA GCTGTGCGCC AAATGAATCC ACATATCCGG GTGACAAGCC ACCAGAACCG 
     4981 TGTGGGTCCT GACACGGAGC GCATCTATGA TGACGATTTT TTCCAAAACC TAGATGGCGT 
     5041 GGCCAATGCC CTGGACAACG TGGATGCCCG CATGTACATG GACCGCCGCT GTGTCTACTA 
     5101 CCGGAAGCCA CTGCTGGAGT CAGGCACACT GGGCACCAAA GGCAATGTGC AGGTGGTGAT 
     5161 CCCCTTCCTG ACAGAGTCGT ACAGTTCCAG CCAGGACCCA CCTGAGAAGT CCATCCCCAT 
     5221 CTGTACCCTG AAGAACTTCC CTAATGCCAT CGAGCACACC CTGCAGTGGG CTCGGGATGA 
     5281 GTTTGAAGGC CTCTTCAAGC AGCCAGCAGA AAATGTCAAC CAGTACCTCA CAGACCCCAA 
     5341 GTTTGTGGAG CGAACACTGC GGCTGGCAGG CACTCAGCCC TTGGAGGTGC TGGAGGCTGT 
     5401 GCAGCGCAGC CTGGTGCTGC AGCGACCACA GACCTGGGCT GACTGCGTGA CCTGGGCCTG 
     5461 CCACCACTGG CACACCCAGT ACTCGAACAA CATCCGGCAG CTGCTGCACA ACTTCCCTCC 
     5521 TGACCAGCTC ACAAGCTCAG GAGCGCCGTT CTGGTCTGGG CCCAAACGCT GTCCACACCC 
     5581 GCTCACCTTT GATGTCAACA ATCCCCTGCA TCTGGACTAT GTGATGGCTG CTGCCAACCT 
     5641 GTTTGCCCAG ACCTACGGGC TGACAGGCTC TCAGGACCGA GCTGCTGTGG CCACATTCCT 
     5701 GCAGTCTGTG CAGGTCCCCG AATTCACCCC CAAGTCTGGC GTCAAGATCC ATGTTTCTGA 
     5761 CCAGGAGCTG CAGAGCGCCA ATGCCTCTGT TGATGACAGT CGTCTAGAGG AGCTCAAAGC 
     5821 CACTCTGCCC AGCCCAGACA AGCTCCCTGG ATTCAAGATG TACCCCATTG ACTTTGAGAA 
     5881 GGATGATGAC AGCAACTTTC ATATGGATTT CATCGTGGCT GCATCCAACC TCCGGGCAGA 
     5941 AAACTATGAC ATTCCTTCTG CAGACCGGCA CAAGAGCAAG CTGATTGCAG GGAAGATCAT 
     6001 CCCAGCCATT GCCACGACCA CAGCAGCCGT GGTTGGCCTT GTGTGTCTGG AGCTGTACAA 
     6061 GGTTGTGCAG GGGCACCGAC AGCTTGACTC CTACAAGAAT GGTTTCCTCA ACTTGGCCCT 
     6121 GCCTTTCTTT GGTTTCTCTG AACCCCTTGC CGCACCACGT CACCAGTACT ATAACCAAGA 
     6181 GTGGACATTG TGGGATCGCT TTGAGGTACA AGGGCTGCAG CCTAATGGTG AGGAGATGAC 
     6241 CCTCAAACAG TTCCTCGACT ATTTTAAGAC AGAGCACAAA TTAGAGATCA CCATGCTGTC 
     6301 CCAGGGCGTG TCCATGCTCT ATTCCTTCTT CATGCCAGCT GCCAAGCTCA AGGAACGGTT 
     6361 GGATCAGCCG ATGACAGAGA TTGTGAGCCG TGTGTCGAAG CGAAAGCTGG GCCGCCACGT 
     6421 GCGGGCGCTG GTGCTTGAGC TGTGCTGTAA CGACGAGAGC GGCGAGGATG TCGAGGTTCC 
     6481 CTATGTCCGA TACACCATCC GCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA 
     6541 AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC 
     6601 GGCCGGCCGC GGTCTGTACA AGTAGGATTC GTCGAGGGAC CTAATAACTT CGTATAGCAT 
     6661 ACATTATACG AAGTTATACA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC 
     6721 AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT 
     6781 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT 
     6841 ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT 
     6901 TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC 
     6961 GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT 
     7021 TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA 
     7081 GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT 
     7141 CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC 
     7201 CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT 
     7261 CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG 
     7321 CATCGATACC GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA 
     7381 TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG 
     7441 TTTTCCAGTC ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT 
     7501 TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA 
     7561 AGATATCCTT GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA 
     7621 CACACCAGGG CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC 
     7681 AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC 
     7741 TGTGAGCCTG CATGGGATGG ATGACCCGGA GAGAGAAGTA TTAGAGTGGA GGTTTGACAG 
     7801 CCGCCTAGCA TTTCATCACA TGGCCCGAGA GCTGCATCCG GACTGTACTG GGTCTCTCTG 
     7861 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC 
     7921 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG 
     7981 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GCATGTGAGC 
     8041 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     8101 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     8161 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     8221 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     8281 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     8341 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     8401 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     8461 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     8521 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     8581 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     8641 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     8701 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGATTAC 
     8761 GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG 
     8821 AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT 
     8881 TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG 
     8941 TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA 
     9001 ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT 
     9061 ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA 
     9121 GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG 
     9181 TCTTTCATTG CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA 
     9241 AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC 
     9301 AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT 
     9361 TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATACTC 
     9421 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     9481 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTCCCGC GCACATTTCC CCGAAAAGTG 
     9541 CCACCTGAC
//