LOCUS       RC219452                8316 bp    DNA     circular  SYN 
DEFINITION  CACNA2D2 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 2 (CACNA2D2), transcript variant 1
ACCESSION   NM_001005505
VERSION     NM_001005505.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4473..4564 
                     /label="Myc-DDK"  
     misc_feature    4594..5179 
                     /label="PolyA signal"  
     misc_feature    4651..4670 
                     /label="Restriction Enzyme"  
     misc_feature    5455..6127 
                     /label="ColE1"  
     misc_feature    6557..7351 
                     /label="Kan/Neo"  
     misc_feature    7386..7688 
                     /label="SV40 ori"  
     misc_feature    7774..8251 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4455..4461 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4455 
                     /label="CACNA2D2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGCCG GCTCGGACCT GCGGCGCCTC TCGGCCCGGC CCAGCGCGGA 
     1081 CTGCGCGCCC CTGGCCCGGC TGCGGCCCCC ACCCTGGCCC CGGCACCCGG CGCCCGACGT 
     1141 CCGGGCCCCC GCGCCCGCTG TGGCTGCTGC TGCCGCTTCT ACCGCTGCTC GCCGCCCCCG 
     1201 GCGCCTCTGC CTACAGCTTC CCCCAGCAGC ACACGATGCA GCACTGGGCC CGGCGTCTGG 
     1261 AGCAGGAGGT CGACGGCGTG ATGCGGATTT TTGGAGGCGT CCAGCAGCTC CGTGAGATTT 
     1321 ACAAGGACAA CCGGAACCTG TTCGAGGTAC AGGAGAATGA GCCTCAGAAG TTGGTGGAGA 
     1381 AGGTGGCAGG GGACATTGAG AGCCTTCTGG ACAGGAAGGT GCAGGCCCTG AAGAGACTGG 
     1441 CTGATGCTGC AGAGAACTTC CAGAAAGCAC ACCGCTGGCA GGACAACATC AAGGAGGAAG 
     1501 ACATCGTGTA CTATGACGCC AAGGCTGACG CTGAGCTGGA CGACCCTGAG AGTGAGGATG 
     1561 TGGAAAGGGG GTCTAAGGCC AGCACCCTAA GGCTGGACTT CATCGAGGAC CCAAACTTCA 
     1621 AGAACAAGGT CAACTATTCA TACGCGGCTG TACAGATCCC TACGGACATC TACAAAGGCT 
     1681 CCACTGTCAT CCTCAATGAG CTCAACTGGA CAGAGGCCCT GGAGAATGTG TTCATGGAAA 
     1741 ACCGCAGACA AGACCCCACA CTGCTGTGGC AGGTCTTCGG CAGCGCCACA GGAGTCACTC 
     1801 GCTACTACCC GGCCACCCCG TGGCGAGCCC CCAAGAAGAT CGACCTGTAC GATGTCCGAA 
     1861 GGAGACCCTG GTATATCCAG GGGGCCTCGT CACCCAAAGA CATGGTCATC ATCGTGGATG 
     1921 TGAGTGGCAG TGTGAGCGGC CTGACCCTGA AGCTGATGAA GACATCTGTC TGCGAGATGC 
     1981 TGGACACGCT GTCTGATGAT GACTATGTGA ATGTGGCCTC GTTCAACGAG AAGGCACAGC 
     2041 CTGTGTCATG CTTCACACAC CTGGTGCAGG CCAATGTGCG CAACAAGAAG GTGTTCAAGG 
     2101 AAGCTGTGCA GGGCATGGTG GCCAAGGGCA CCACAGGCTA CAAGGCCGGC TTTGAGTATG 
     2161 CCTTTGACCA GCTGCAGAAC TCCAACATCA CTCGGGCCAA CTGCAACAAG ATGATCATGA 
     2221 TGTTCACGGA TGGTGGTGAG GACCGCGTGC AGGACGTCTT TGAGAAGTAC AATTGGCCAA 
     2281 ACCGGACGGT GCGCGTGTTT ACTTTCTCCG TGGGGCAGCA TAACTATGAC GTCACACCGC 
     2341 TGCAGTGGAT GGCCTGTGCC AACAAAGGCT ACTATTTTGA GATCCCTTCC ATCGGAGCCA 
     2401 TCCGCATCAA CACACAGGAA TATCTAGATG TGTTGGGCAG GCCCATGGTG CTGGCAGGCA 
     2461 AGGAGGCCAA GCAGGTGCAG TGGACCAACG TGTATGAGGA TGCACTGGGA CTGGGGTTGG 
     2521 TGGTAACAGG GACCCTCCCT GTTTTCAACC TGACACAGGA TGGCCCTGGG GAAAAGAAGA 
     2581 ACCAGCTGAT CCTGGGCGTG ATGGGCATTG ACGTGGCTCT GAATGACATC AAGAGGCTGA 
     2641 CCCCCAACTA CACGCTTGGA GCCAACGGCT ATGTGTTTGC CATTGACCTG AACGGCTACG 
     2701 TGTTGCTGCA CCCCAATCTC AAGCCCCAGA CCACCAACTT CCGGGAGCCT GTGACTCTGG 
     2761 ACTTCCTGGA TGCGGAGCTA GAGGATGAGA ACAAGGAAGA GATCCGTCGG AGCATGATTG 
     2821 ATGGCAACAA GGGCCACAAG CAGATCAGAA CGTTGGTCAA GTCCCTGGAT GAGAGGTACA 
     2881 TAGATGAGGT GACACGGAAC TACACCTGGG TGCCTATAAG GAGCACTAAC TACAGCCTGG 
     2941 GGCTGGTGCT CCCACCCTAC AGCACCTTCT ACCTCCAAGC CAATCTCAGT GACCAGATCC 
     3001 TGCAGGTCAA GTATTTTGAG TTCCTGCTCC CCAGCAGCTT TGAGTCTGAA GGACACGTTT 
     3061 TCATTGCTCC CAGAGAGTAC TGCAAGGACC TGAATGCCTC AGACAACAAC ACCGAGTTCC 
     3121 TGAAAAACTT TATTGAGCTC ATGGAGAAAG TGACTCCAGA CTCCAAGCAG TGCAACAACT 
     3181 TCCTTCTGCA CAACCTGATC TTGGACACGG GCATCACGCA GCAGCTGGTA GAGCGTGTGT 
     3241 GGAGGGACCA GGATCTCAAC ACGTACAGCC TACTGGCCGT GTTCGCTGCC ACAGACGGTG 
     3301 GCATCACCCG AGTCTTCCCC AACAAGGCAG CTGAGGACTG GACAGAGAAC CCTGAGCCCT 
     3361 TCAATGCCAG CTTCTACCGC CGCAGCCTGG ATAACCACGG TTATGTCTTC AAGCCCCCAC 
     3421 ACCAGGATGC CCTGTTAAGG CCGCTGGAGC TGGAGAATGA CACTGTGGGC ATCCTCGTCA 
     3481 GCACAGCTGT GGAGCTCAGC CTAGGCAGGC GCACACTGAG GCCAGCAGTG GTGGGCGTCA 
     3541 AGCTGGACCT AGAGGCTTGG GCTGAGAAGT TCAAGGTGCT AGCCAGCAAC CGTACCCACC 
     3601 AAGACCAGCC TCAGAAGTGC GGCCCCAACA GCCACTGTGA GATGGACTGC GAGGTTAACA 
     3661 ATGAGGACTT ACTCTGTGTC CTCATTGATG ATGGAGGATT CCTGGTGCTG TCAAACCAGA 
     3721 ACCATCAGTG GGACCAGGTG GGCAGGTTCT TCAGTGAGGT GGATGCCAAC CTGATGCTGG 
     3781 CACTCTACAA TAACTCCTTC TACACCCGCA AGGAGTCCTA TGACTATCAG GCAGCCTGTG 
     3841 CCCCTCAGCC CCCTGGCAAC CTGGGTGCTG CACCCCGGGG TGTCTTTGTG CCCACCGTTG 
     3901 CAGATTTCCT TAACCTGGCC TGGTGGACCT CTGCTGCCGC CTGGTCCCTG TTCCAGCAGC 
     3961 TTCTCTACGG CCTCATCTAC CACAGCTGGT TCCAAGCAGA CCCCGCGGAG GCCGAGGGGA 
     4021 GCCCCGAGAC GCGCGAGAGC AGCTGCGTCA TGAAACAGAC CCAGTACTAC TTCGGCTCGG 
     4081 TAAACGCCTC CTACAACGCC ATCATCGACT GCGGAAACTG CTCCAGGCTG TTCCACGCGC 
     4141 AGAGACTGAC CAACACCAAT CTTCTCTTTG TGGTGGCCGA GAAGCCGCTG TGCAGCCAGT 
     4201 GCGAGGCTGG CCGGCTGCTG CAGAAGGAGA CGCACTGCCC AGCGGACGGC CCGGAGCAGT 
     4261 GTGAGCTAGT GCAGAGACCG CGATACCGGA GAGGCCCGCA CATCTGCTTC GACTACAACG 
     4321 CGACAGAAGA TACCTCAGAC TGTGGCCGCG GGGCCTCCTT CCCGCCGTCG CTGGGCGTCC 
     4381 TGGTCTCCCT GCAACTGCTG CTCCTCCTGG GCCTGCCGCC CCGGCCGCAG CCTCAAGTCC 
     4441 TCGTCCACGC CTCTCGCCGC CTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4501 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4561 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4621 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4681 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4741 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4801 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4861 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4921 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4981 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5041 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5101 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5161 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5221 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5281 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5341 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5401 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5461 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5521 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5581 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5641 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5701 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5761 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5821 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5881 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5941 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6001 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6061 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6121 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6181 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6241 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6301 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6361 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6421 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6481 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6541 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6601 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6661 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6721 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6781 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6841 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6901 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6961 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7021 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7081 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7141 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7201 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7261 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7321 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7381 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7441 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7501 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7561 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7681 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7741 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7801 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7861 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7921 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7981 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8041 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8101 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8161 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8221 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8281 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//