LOCUS       RC219433                7189 bp    DNA     circular  SYN 
DEFINITION  ANKEF1 (Myc-DDK-tagged)-Human ankyrin repeat domain 5 (ANKRD5), transcript variant 1
ACCESSION   NM_022096
VERSION     NM_022096.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3344..3435 
                     /label="Myc-DDK"  
     misc_feature    3465..4050 
                     /label="PolyA signal"  
     misc_feature    3522..3541 
                     /label="Restriction Enzyme"  
     misc_feature    4326..4998 
                     /label="ColE1"  
     misc_feature    5428..6222 
                     /label="Kan/Neo"  
     misc_feature    6257..6559 
                     /label="SV40 ori"  
     misc_feature    6645..7122 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3319..3326 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3319 
                     /label="ANKEF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTAGCA GATAAGAGAC TTGAGAACTT ACAGATCTAC AAAGTTCTTC 
     1081 AATGTGTGCG GAACAAAGAC AAGAAGCAGA TAGAGAAGCT GACCAAGCTT GGATACCCTG 
     1141 AACTAATCAA TTATACAGAA CCCATTAATG GACTTAGTGC TTTGCACTTA GCCTCAGTTT 
     1201 CCAATGATAT TGATATGGTC AGCTTTCTCC TTGACCTTGG TGCTCACCCT GATGTGCAAG 
     1261 ACCGAATGGG CTGTACTCCC ACAATGAGGG CTGCAGAACT GGGCCATGAA TTGTCAATGG 
     1321 AAATATTAGC AAAGGCAAAG GCTGATATGA CTATAGTTGA TAATGAAGGA AAAGGTGTTT 
     1381 TGTTTTACTG CATTTTACCG ACTAAGCGGC ATTATCGCTG TGCTCTGATC GCCCTTGAAC 
     1441 ATGGTGCAGA TGTCAACAAT TCTACCTATG AAGGAAAGCC AATATTCCTT AGAGCTTGTG 
     1501 AAGATGCACA TGATGTTAAA GATGTGTGCC TGACATTTTT GGAAAAAGGA GCCAATCCTA 
     1561 ATGCAATCAA CTCATCCACA GGCCGCACAG CTTTAATGGA AGCGTCAAGA GAAGGGGTAG 
     1621 TGGAAATAGT TCGAGGCATA TTGGAAAGAG GAGGTGAAGT GAATGCATTT GACAACGACA 
     1681 GGCATCACGC TGCTCATTTT GCTGCTAAAG GAGGCTTTTT CGATATATTG AAGCTTCTTT 
     1741 TTGCCTACAA TGGAGACGTG GGGCTGATTT CGATAAATGG GAACACACCA CTTCATTATG 
     1801 CTGCCATGGG TGGTTTTGCA GACTGCTGTA AATATATAGC TCAGCGAGGA TGTGACCTGA 
     1861 AATGGAAGAA TTTAGATCAT AAAACGCCCA GGGCTGTGGC TAAGGAAGGC GGCTTCAAAG 
     1921 CAGCAAGCAA AGAAATACGC CGAGCAGAGA GAATCGCTAA TAAACTAGCC AGGCCAGGAG 
     1981 CCAAAAATCC AAATCCACTG TGGGCCCTTA GACTGCACGA TTGGTCCGTA GAACGTGAGG 
     2041 CTTTCCTCCG GGAAGCCTTT GCGGTTTTAG ACAGGGGTGA TGGAAGCATC AGCAAGAACG 
     2101 ACTTCGTGAT GGTGTTGGAG GAAAGGCAGG ATTATGCAAG CTCAGAACAG CTGGCTGCCA 
     2161 TCGCTCACCT TCATGAGAAA ACCCGGGGAG GAGGGGTCAA TATTAATGAA TTCTTTAAAG 
     2221 GAACCAGATA TTTAAACAAG TCTTTTGTCT TAGGATCGTA TGGACCTAAG AAAAAGGAAA 
     2281 AAGGGATGGG CAAAAAAGGA AAGAAAGGGA AATTTGTCTT ACCCCTTCCA ATCTGTGTCA 
     2341 TTCCTGAGTA CGCGTTTCCA CGCCGGCAGG ATGGTGGGCC ACCGTATTAC ATGATTGAGA 
     2401 CCTACAAGAA TGTCACTGAT AGCAGCCGGT TTAATAGAGA TCATCCCCCA GAACATCCCA 
     2461 TTCAGGATGA CTCTGTTTGG TACATTGATG ATTCAGAGAA GGTATTTTCA AACATTAATA 
     2521 TTATCACCAA AGCAGGGGAT CTGGCTTCTC TGAAAAAGGC CTTTGAATCA GGAATACCTG 
     2581 TGGATATGAA GGATAATTAT TACAAAACTC CGCTAATGAC GGCGTGTGCA AGTGGAAACA 
     2641 TAGATGTGGT CAAGTTTCTT CTTGAAAAAG GAGCTAACGT TAATGCAACA GATAACTTTC 
     2701 TGTGGACTCC ACTTCATTTT GCATGCCATG CAGGCCAACA AGACATTGTT GAGCTTCTTG 
     2761 TTGAATCTGG AGCTTTAATA GATGCAGCTT CAATCAACAA CTCAACTCCT TTAAATAGAG 
     2821 CCATTGAAAG CTGCAGACTG GATACAGTAA AATACCTACT TGATATTGGT GCTAAATTCC 
     2881 AGCTGGAAAA TAGAAAAGGG CATAGTGCCA TGGACGTTGC AAAGGCATAT GCTGATTATA 
     2941 GAATAATTGA TCTGATTAAA GAAAAGCTAG ATAACTTGCC GAAACCAGCA GAAAATCAAA 
     3001 AACTAAAAGG CAAGACACCT CCTATACTGA AGACTGAAGG CCCTGAAATT AAGAAAGAAG 
     3061 AGGAACTGCT GTCATCAATT TATGGTGTAC CAACCACATC AGAGGGAAAG AAAGTACAGA 
     3121 AGGGTAATGT GGTTCATCTG AATTCATTGA TTACCAGTGG TTATACTAAG AAAGTGGATA 
     3181 TCACATTTAT TCCACGGAGG ATTTGGAGTC CTGAAGCCAC AACAGCAGAG CTGATCAGGA 
     3241 AGAGGGAACT ACGGCGAGAG AGGTTTACAC ATGAGGTGGA CTTCGACGAT TTTATGATGC 
     3301 CTTTTCAGAA GAACATCACA GAGAAAGAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG 
     3361 AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC 
     3421 GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA 
     3481 TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC 
     3541 CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA 
     3601 TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG 
     3661 GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC 
     3721 AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT 
     3781 CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC 
     3841 CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC 
     3901 CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA 
     3961 ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC 
     4021 GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA 
     4081 GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     4141 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     4201 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     4261 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     4321 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     4381 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     4441 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     4501 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     4561 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     4621 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     4681 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     4741 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 
     4801 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     4861 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     4921 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     4981 ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC 
     5041 CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC 
     5101 AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT 
     5161 GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG 
     5221 TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA 
     5281 ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC 
     5341 CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG 
     5401 GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG 
     5461 GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG 
     5521 GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG 
     5581 ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC 
     5641 CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG 
     5701 CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC 
     5761 CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG 
     5821 TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC 
     5881 ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC 
     5941 CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC 
     6001 TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC 
     6061 ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG 
     6121 CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG 
     6181 CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA 
     6241 CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC 
     6301 CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA 
     6361 AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG 
     6421 GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC 
     6481 TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC 
     6541 TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT 
     6601 TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA 
     6661 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 
     6721 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT 
     6781 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC 
     6841 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 
     6901 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 
     6961 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT 
     7021 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC 
     7081 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 
     7141 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//