LOCUS       RC219405                7977 bp    DNA     circular  SYN 
DEFINITION  KIF5A (Myc-DDK-tagged)-Human kinesin family member 5A (KIF5A)
ACCESSION   NM_004984
VERSION     NM_004984.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4134..4225 
                     /label="Myc-DDK"  
     misc_feature    4255..4840 
                     /label="PolyA signal"  
     misc_feature    4312..4331 
                     /label="Restriction Enzyme"  
     misc_feature    5116..5788 
                     /label="ColE1"  
     misc_feature    6218..7012 
                     /label="Kan/Neo"  
     misc_feature    7047..7349 
                     /label="SV40 ori"  
     misc_feature    7435..7912 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4116..4122 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4116 
                     /label="KIF5A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGACC AACAACGAAT GTAGCATCAA GGTGCTCTGC CGATTCCGGC 
     1081 CCCTGAACCA GGCTGAGATT CTGCGGGGAG ACAAGTTCAT CCCCATTTTC CAAGGGGACG 
     1141 ACAGCGTCGT TATTGGGGGG AAGCCATATG TTTTTGACCG TGTATTCCCC CCAAACACGA 
     1201 CTCAAGAGCA AGTTTATCAT GCATGTGCCA TGCAGATTGT CAAAGATGTC CTTGCTGGCT 
     1261 ACAATGGCAC CATTTTTGCT TATGGACAGA CATCCTCAGG GAAAACACAT ACCATGGAGG 
     1321 GAAAGCTGCA CGACCCTCAG CTGATGGGAA TCATTCCTCG AATTGCCCGA GACATCTTCA 
     1381 ACCACATCTA CTCCATGGAT GAGAACCTTG AGTTCCACAT CAAGGTTTCT TACTTTGAAA 
     1441 TTTACCTGGA CAAAATTCGT GACCTTCTGG ATGTGACCAA GACAAATCTG TCCGTGCACG 
     1501 AGGACAAGAA CCGGGTGCCA TTTGTCAAGG GTTGTACTGA ACGCTTTGTG TCCAGCCCGG 
     1561 AGGAGATTCT GGATGTGATT GATGAAGGGA AATCAAATCG TCATGTGGCT GTCACCAACA 
     1621 TGAATGAACA CAGCTCTCGG AGCCACAGCA TCTTCCTCAT CAACATCAAG CAGGAGAACA 
     1681 TGGAAACGGA GCAGAAGCTC AGTGGGAAGC TGTATCTGGT GGACCTGGCA GGGAGTGAGA 
     1741 AGGTCAGCAA GACTGGAGCA GAGGGAGCCG TGCTGGACGA GGCAAAGAAT ATCAACAAGT 
     1801 CACTGTCAGC TCTGGGCAAT GTGATCTCCG CACTGGCTGA GGGCACTAAA AGCTATGTTC 
     1861 CATATCGTGA CAGCAAAATG ACAAGGATTC TCCAGGACTC TCTCGGGGGA AACTGCCGGA 
     1921 CGACTATGTT CATCTGTTGC TCACCATCCA GTTATAATGA TGCAGAGACC AAGTCCACCC 
     1981 TGATGTTTGG GCAGCGGGCA AAGACCATTA AGAACACTGC CTCAGTAAAT TTGGAGTTGA 
     2041 CTGCTGAGCA GTGGAAGAAG AAATATGAGA AGGAGAAGGA GAAGACAAAG GCCCAGAAGG 
     2101 AGACGATTGC GAAGCTGGAG GCTGAGCTGA GCCGGTGGCG CAATGGAGAG AATGTGCCTG 
     2161 AGACAGAGCG CCTGGCTGGG GAGGAGGCAG CCCTGGGAGC CGAGCTCTGT GAGGAGACCC 
     2221 CTGTGAATGA CAACTCATCC ATCGTGGTGC GCATCGCGCC CGAGGAGCGG CAGAAATACG 
     2281 AGGAGGAGAT CCGCCGTCTC TATAAGCAGC TTGACGACAA GGATGATGAA ATCAACCAAC 
     2341 AAAGCCAACT CATAGAGAAG CTCAAGCAGC AAATGCTGGA CCAGGAAGAG CTGCTGGTGT 
     2401 CCACCCGAGG AGACAACGAG AAGGTCCAGC GGGAGCTGAG CCACCTGCAA TCAGAGAACG 
     2461 ATGCCGCTAA GGATGAGGTG AAGGAAGTGC TGCAGGCCCT GGAGGAGCTG GCTGTGAACT 
     2521 ATGACCAGAA GTCCCAGGAG GTGGAGGAGA AGAGCCAGCA GAACCAGCTT CTGGTGGATG 
     2581 AGCTGTCTCA GAAGGTGGCC ACCATGCTGT CCCTGGAGTC TGAGTTGCAG CGGCTACAGG 
     2641 AGGTCAGTGG ACACCAGCGA AAACGAATTG CTGAGGTGCT GAACGGGCTG ATGAAGGATC 
     2701 TGAGCGAGTT CAGTGTCATT GTGGGCAACG GGGAGATTAA GCTGCCAGTG GAGATCAGTG 
     2761 GGGCCATCGA GGAGGAGTTC ACTGTGGCCC GACTCTACAT CAGCAAAATC AAATCAGAAG 
     2821 TCAAGTCTGT GGTCAAGCGG TGCCGGCAGC TGGAGAACCT CCAGGTGGAG TGTCACCGCA 
     2881 AGATGGAAGT GACCGGGCGG GAGCTCTCAT CCTGCCAGCT CCTCATCTCT CAGCATGAGG 
     2941 CCAAGATCCG CTCGCTTACG GAATACATGC AGAGCGTGGA GCTAAAGAAG CGGCACCTGG 
     3001 AAGAGTCCTA TGACTCCTTG AGCGATGAGC TGGCCAAGCT CCAGGCCCAG GAAACTGTGC 
     3061 ATGAAGTGGC CCTGAAGGAC AAGGAGCCTG ACACTCAGGA TGCAGATGAA GTGAAGAAGG 
     3121 CTCTGGAGCT GCAGATGGAG AGTCACCGGG AGGCCCATCA CCGGCAGCTG GCCCGGCTCC 
     3181 GGGACGAGAT CAACGAGAAG CAGAAGACCA TTGATGAGCT CAAAGACCTA AATCAGAAGC 
     3241 TCCAGTTAGA GCTAGAGAAG CTTCAGGCTG ACTACGAGAA GCTGAAGAGC GAAGAACACG 
     3301 AGAAGAGCAC CAAGCTGCAG GAGCTGACAT TTCTGTACGA GCGACATGAG CAGTCCAAGC 
     3361 AGGACCTCAA GGGTCTGGAG GAGACAGTTG CCCGGGAACT CCAGACCCTC CACAACCTTC 
     3421 GCAAGCTGTT CGTTCAAGAC GTCACGACTC GAGTCAAGAA AAGTGCAGAA ATGGAGCCCG 
     3481 AAGACAGTGG GGGGATTCAC TCCCAAAAGC AGAAGATTTC CTTTCTTGAG AACAACCTGG 
     3541 AACAGCTTAC AAAGGTTCAC AAACAGCTGG TACGTGACAA TGCAGATCTG CGTTGTGAGC 
     3601 TTCCTAAATT GGAAAAACGA CTTAGGGCTA CGGCTGAGAG AGTTAAGGCC CTGGAGGGTG 
     3661 CACTGAAGGA GGCCAAGGAG GGCGCCATGA AGGACAAGCG CCGGTACCAG CAGGAGGTGG 
     3721 ACCGCATCAA GGAGGCCGTT CGCTACAAGA GCTCGGGCAA ACGGGGCCAT TCTGCCCAGA 
     3781 TTGCCAAACC CGTCCGGCCT GGCCACTACC CAGCATCCTC ACCCACCAAC CCCTATGGCA 
     3841 CCCGGAGCCC TGAGTGCATC AGTTACACCA ACAGCCTCTT CCAGAACTAC CAGAATCTCT 
     3901 ACCTGCAGGC CACACCCAGC TCCACCTCAG ATATGTACTT TGCAAACTCC TGTACCAGCA 
     3961 GTGGAGCCAC ATCTTCTGGC GGCCCCTTGG CTTCCTACCA GAAGGCCAAC ATGGACAATG 
     4021 GAAATGCCAC AGATATCAAT GACAATAGGA GTGACCTGCC GTGTGGCTAT GAGGCTGAGG 
     4081 ACCAGGCCAA GCTTTTCCCT CTCCACCAAG AGACAGCAGC CAGCACGCGT ACGCGGCCGC 
     4141 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4201 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4261 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4321 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4381 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4441 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4501 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4561 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4621 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4681 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4741 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4801 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4861 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4921 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4981 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5041 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5101 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5161 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5221 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5281 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5341 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5401 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5461 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5521 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5581 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5641 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5701 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5761 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5821 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5881 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5941 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6001 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6061 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6121 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6181 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6241 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6301 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6361 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6421 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6481 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6541 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6601 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6661 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6721 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6781 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6841 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6901 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6961 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7021 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7081 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7141 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7201 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7261 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7321 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7381 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7441 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7501 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7561 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7621 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7681 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7741 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7801 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7861 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7921 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//