LOCUS       RC219404                7704 bp    DNA     circular  SYN 
DEFINITION  GABBR2 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) B receptor, 2 (GABBR2)
ACCESSION   NM_005458
VERSION     NM_005458.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3861..3952 
                     /label="Myc-DDK"  
     misc_feature    3982..4567 
                     /label="PolyA signal"  
     misc_feature    4039..4058 
                     /label="Restriction Enzyme"  
     misc_feature    4843..5515 
                     /label="ColE1"  
     misc_feature    5945..6739 
                     /label="Kan/Neo"  
     misc_feature    6774..7076 
                     /label="SV40 ori"  
     misc_feature    7162..7639 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3843..3849 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3843 
                     /label="GABBR2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCCCCG CGGAGCTCCG GGCAGCCCGG GCCGCCGCCG CCGCCGCCAC 
     1081 CGCCGCCCGC GCGCCTGCTA CTGCTACTGC TGCTGCCGCT GCTGCTGCCT CTGGCGCCCG 
     1141 GGGCCTGGGG CTGGGCGCGG GGCGCCCCCC GGCCGCCGCC CAGCAGCCCG CCGCTCTCCA 
     1201 TCATGGGCCT CATGCCGCTC ACCAAGGAGG TGGCCAAGGG CAGCATCGGG CGCGGTGTGC 
     1261 TCCCCGCCGT GGAACTGGCC ATCGAGCAGA TCCGCAACGA GTCACTCCTG CGCCCCTACT 
     1321 TCCTCGACCT GCGGCTCTAT GACACGGAGT GCGACAACGC AAAAGGGTTG AAAGCCTTCT 
     1381 ACGATGCAAT AAAATACGGG CCGAACCACT TGATGGTGTT TGGAGGCGTC TGTCCATCCG 
     1441 TCACATCCAT CATTGCAGAG TCCCTCCAAG GCTGGAATCT GGTGCAGCTT TCTTTTGCTG 
     1501 CAACCACGCC TGTTCTAGCC GATAAGAAAA AATACCCTTA TTTCTTTCGG ACCGTCCCAT 
     1561 CAGACAATGC GGTGAATCCA GCCATTCTGA AGTTGCTCAA GCACTACCAG TGGAAGCGCG 
     1621 TGGGCACGCT GACGCAAGAC GTTCAGAGGT TCTCTGAGGT GCGGAATGAC CTGACTGGAG 
     1681 TTCTGTATGG CGAGGACATT GAGATTTCAG ACACCGAGAG CTTCTCCAAC GATCCCTGTA 
     1741 CCAGTGTCAA AAAGCTGAAG GGGAATGATG TGCGGATCAT CCTTGGCCAG TTTGACCAGA 
     1801 ATATGGCAGC AAAAGTGTTC TGTTGTGCAT ACGAGGAGAA CATGTATGGT AGTAAATATC 
     1861 AGTGGATCAT TCCGGGCTGG TACGAGCCTT CTTGGTGGGA GCAGGTGCAC ACGGAAGCCA 
     1921 ACTCATCCCG CTGCCTCCGG AAGAATCTGC TTGCTGCCAT GGAGGGCTAC ATTGGCGTGG 
     1981 ATTTCGAGCC CCTGAGCTCC AAGCAGATCA AGACCATCTC AGGAAAGACT CCACAGCAGT 
     2041 ATGAGAGAGA GTACAACAAC AAGCGGTCAG GCGTGGGGCC CAGCAAGTTC CACGGGTACG 
     2101 CCTACGATGG CATCTGGGTC ATCGCCAAGA CACTGCAGAG GGCCATGGAG ACACTGCATG 
     2161 CCAGCAGCCG GCACCAGCGG ATCCAGGACT TCAACTACAC GGACCACACG CTGGGCAGGA 
     2221 TCATCCTCAA TGCCATGAAC GAGACCAACT TCTTCGGGGT CACGGGTCAA GTTGTATTCC 
     2281 GGAATGGGGA GAGAATGGGG ACCATTAAAT TTACTCAATT TCAAGACAGC AGGGAGGTGA 
     2341 AGGTGGGAGA GTACAACGCT GTGGCCGACA CACTGGAGAT CATCAATGAC ACCATCAGGT 
     2401 TCCAAGGATC CGAACCACCA AAAGACAAGA CCATCATCCT GGAGCAGCTG CGGAAGATCT 
     2461 CCCTACCTCT CTACAGCATC CTCTCTGCCC TCACCATCCT CGGGATGATC ATGGCCAGTG 
     2521 CTTTTCTCTT CTTCAACATC AAGAACCGGA ATCAGAAGCT CATAAAGATG TCGAGTCCAT 
     2581 ACATGAACAA CCTTATCATC CTTGGAGGGA TGCTCTCCTA TGCTTCCATA TTTCTCTTTG 
     2641 GCCTTGATGG ATCCTTTGTC TCTGAAAAGA CCTTTGAAAC ACTTTGCACC GTCAGGACCT 
     2701 GGATTCTCAC CGTGGGCTAC ACGACCGCTT TTGGGGCCAT GTTTGCAAAG ACCTGGAGAG 
     2761 TCCACGCCAT CTTCAAAAAT GTGAAAATGA AGAAGAAGAT CATCAAGGAC CAGAAACTGC 
     2821 TTGTGATCGT GGGGGGCATG CTGCTGATCG ACCTGTGTAT CCTGATCTGC TGGCAGGCTG 
     2881 TGGACCCCCT GCGAAGGACA GTGGAGAAGT ACAGCATGGA GCCGGACCCA GCAGGACGGG 
     2941 ATATCTCCAT CCGCCCTCTC CTGGAGCACT GTGAGAACAC CCATATGACC ATCTGGCTTG 
     3001 GCATCGTCTA TGCCTACAAG GGACTTCTCA TGTTGTTCGG TTGTTTCTTA GCTTGGGAGA 
     3061 CCCGCAACGT CAGCATCCCC GCACTCAACG ACAGCAAGTA CATCGGGATG AGTGTCTACA 
     3121 ACGTGGGGAT CATGTGCATC ATCGGGGCCG CTGTCTCCTT CCTGACCCGG GACCAGCCCA 
     3181 ATGTGCAGTT CTGCATCGTG GCTCTGGTCA TCATCTTCTG CAGCACCATC ACCCTCTGCC 
     3241 TGGTATTCGT GCCGAAGCTC ATCACCCTGA GAACAAACCC AGATGCAGCA ACGCAGAACA 
     3301 GGCGATTCCA GTTCACTCAG AATCAGAAGA AAGAAGATTC TAAAACGTCC ACCTCGGTCA 
     3361 CCAGTGTGAA CCAAGCCAGC ACATCCCGCC TGGAGGGCCT ACAGTCAGAA AACCATCACC 
     3421 TGCGAATGAA GATCACAGAG CTGGATAAAG ACTTGGAAGA GGTCACCATG CAGCTGCAGG 
     3481 ACACACCAGA AAAGACCACC TACATTAAAC AGAACCACTA CCAAGAGCTC AATGACATCC 
     3541 TCAACCTGGG AAACTTCACT GAGAGCACAG ATGGAGGAAA GGCCATTTTA AAAAATCACC 
     3601 TCGATCAAAA TCCCCAGCTA CAGTGGAACA CAACAGAGCC CTCTCGAACA TGCAAAGATC 
     3661 CTATAGAAGA TATAAACTCT CCAGAACACA TCCAGCGTCG GCTGTCCCTC CAGCTCCCCA 
     3721 TCCTCCACCA CGCCTACCTC CCATCCATCG GAGGCGTGGA CGCCAGCTGT GTCAGCCCCT 
     3781 GCGTCAGCCC CACCGCCAGC CCCCGCCACA GACATGTGCC ACCCTCCTTC CGAGTCATGG 
     3841 TCTCGGGCCT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3901 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3961 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4021 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4081 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4141 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4201 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4261 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4321 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4381 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4441 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4501 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4561 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4621 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4681 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4741 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4801 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4861 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4921 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4981 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5041 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5101 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5161 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5221 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5281 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5341 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5401 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5461 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5521 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5581 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5641 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5701 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5761 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5821 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5881 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5941 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6001 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6061 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6121 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6181 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6241 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6301 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6361 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6421 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6481 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6541 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6601 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6661 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6721 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6781 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6841 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6901 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6961 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7021 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7081 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7141 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7201 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7261 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7321 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7381 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7441 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7501 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7561 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7621 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7681 TAACAAAAAT TTAACGCGAA TTTT
//