LOCUS       RC219292                9327 bp    DNA     circular  SYN 
DEFINITION  MADD (Myc-DDK-tagged)-Human MAP-kinase activating death domain (MADD), transcript variant 6
ACCESSION   NM_130474
VERSION     NM_130474.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5482..5573 
                     /label="Myc-DDK"  
     misc_feature    5603..6188 
                     /label="PolyA signal"  
     misc_feature    5660..5679 
                     /label="Restriction Enzyme"  
     misc_feature    6464..7136 
                     /label="ColE1"  
     misc_feature    7566..8360 
                     /label="Kan/Neo"  
     misc_feature    8395..8697 
                     /label="SV40 ori"  
     misc_feature    8783..9260 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5457..5464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5457 
                     /label="MADD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCAAAAG AAGAAGTTCT GTCCTCGGTT ACTTGACTAT CTAGTGATCG 
     1081 TAGGGGCCAG GCACCCGAGC AGTGATAGCG TGGCCCAGAC TCCTGAATTG CTACGGCGAT 
     1141 ACCCCTTGGA GGATCACACT GAGTTTCCCC TGCCCCCAGA TGTAGTGTTC TTCTGCCAGC 
     1201 CCGAGGGCTG CCTGAGCGTG CGGCAGCGGC GCATGAGCCT TCGGGATGAT ACCTCTTTTG 
     1261 TCTTCACCCT CACTGACAAG GACACTGGAG TCACGCGATA TGGCATCTGT GTTAACTTCT 
     1321 ACCGCTCCTT CCAAAAGCGA ATCTCTAAGG AGAAGGGGGA AGGTGGGGCA GGGTCCCGTG 
     1381 GGAAGGAAGG AACCCATGCC ACCTGTGCCT CAGAAGAGGG TGGCACTGAG AGCTCAGAGA 
     1441 GTGGCTCATC CCTGCAGCCT CTCAGTGCTG ACTCTACCCC TGATGTGAAC CAGTCTCCTC 
     1501 GGGGCAAACG CCGGGCCAAG GCGGGGAGCC GCTCCCGCAA CAGTACTCTC ACGTCCCTGT 
     1561 GCGTGCTCAG CCACTACCCT TTCTTCTCCA CCTTCCGAGA GTGTTTGTAT ACTCTCAAGC 
     1621 GCCTGGTGGA CTGCTGTAGT GAGCGCCTTC TGGGCAAGAA ACTGGGCATC CCTCGAGGCG 
     1681 TACAAAGGGA CACCATGTGG CGGATCTTTA CTGGATCGCT GCTGGTAGAG GAGAAGTCAA 
     1741 GTGCCCTTCT GCATGACCTT CGAGAGATTG AGGCCTGGAT CTATCGATTG CTGCGCTCCC 
     1801 CAGTACCCGT CTCTGGGCAG AAGCGAGTAG ACATCGAGGT CCTACCCCAA GAGCTCCAGC 
     1861 CAGCTCTGAC CTTTGCTCTT CCAGACCCAT CTCGATTCAC CCTAGTGGAT TTCCCACTGC 
     1921 ACCTTCCCTT GGAACTTCTA GGTGTGGACG CCTGTCTCCA GGTGCTAACC TGCATTCTGT 
     1981 TAGAGCACAA GGTGGTGCTA CAGTCCCGAG ACTACAATGC ACTCTCCATG TCTGTGATGG 
     2041 CATTCGTGGC AATGATCTAC CCACTGGAGT ATATGTTTCC TGTCATCCCG CTGCTACCCA 
     2101 CCTGCATGGC ATCAGCAGAG CAGCTGCTGT TGGCTCCAAC CCCGTACATC ATTGGGGTTC 
     2161 CTGCCAGCTT CTTCCTCTAC AAACTGGACT TCAAAATGCC TGATGATGTA TGGCTAGTGG 
     2221 ATCTGGACAG CAATAGGGTG ATTGCCCCCA CCAATGCAGA AGTGCTGCCT ATCCTGCCAG 
     2281 AACCAGAATC ACTAGAGCTG AAAAAGCATT TAAAGCAGGC CTTGGCCAGC ATGAGTCTCA 
     2341 ACACCCAGCC CATCCTCAAT CTGGAGAAAT TTCATGAGGG CCAGGAGATC CCCCTTCTCT 
     2401 TGGGAAGGCC TTCTAATGAC CTGCAGTCCA CACCGTCCAC TGAATTCAAC CCACTCATCT 
     2461 ATGGCAATGA TGTGGATTCT GTGGATGTTG CAACCAGGGT TGCCATGGTA CGGTTCTTCA 
     2521 ATTCCGCCAA CGTGCTGCAG GGATTTCAGA TGCACACGCG TACCCTGCGC CTCTTTCCTC 
     2581 GGCCTGTGGT AGCTTTTCAA GCTGGCTCCT TTCTAGCCTC ACGTCCCCGG CAGACTCCTT 
     2641 TTGCCGAGAA ATTGGCCAGG ACTCAGGCTG TGGAGTACTT TGGGGAATGG ATCCTTAACC 
     2701 CCACCAACTA TGCCTTTCAG CGAATTCACA ACAATATGTT TGATCCAGCC CTGATTGGTG 
     2761 ACAAGCCAAA GTGGTATGCT CATCAGCTGC AGCCTATCCA CTATCGCGTC TATGACAGCA 
     2821 ATTCCCAGCT GGCTGAGGCC CTGAGTGTAC CACCAGAGCG GGACTCTGAC TCCGAACCTA 
     2881 CTGATGATAG TGGCAGTGAT AGTATGGATT ATGACGATTC AAGCTCTTCT TACTCCTCCC 
     2941 TTGGTGACTT TGTCAGTGAA ATGATGAAAT GTGACATTAA TGGTGATACT CCCAATGTGG 
     3001 ACCCTCTGAC ACATGCAGCA CTGGGGGATG CCAGCGAGGT GGAGATTGAC GAGCTGCAGA 
     3061 ATCAGAAGGA AGCAGAAGAG CCTGGCCCAG ACAGTGAGAA CTCTCAGGAA AACCCCCCAC 
     3121 TGCGCTCCAG CTCTAGCACC ACAGCCAGCA GCAGCCCCAG CACTGTCATC CACGGAGCCA 
     3181 ACTCTGAACC TGCTGACTCT ACGGAGATGG ATGATAAGGC AGCAGTAGGC GTCTCCAAGC 
     3241 CCCTCCCTTC CGTGCCTCCC AGCATTGGCA AATCGAACGT GGACAGACGT CAGGCAGAAA 
     3301 TTGGAGAGGG GGCTCAAAAG CTGCTGCGGC CCAACAGCTT GAGACTGGCA AGTGACTCAG 
     3361 ATGCAGAGTC AGACTCTCGG GCAAGCTCTC CCAACTCCAC CGTCTCCAAC ACCAGCACCG 
     3421 AGGGCTTCGG GGGCATCATG TCTTTTGCCA GCAGCCTCTA TCGGAACCAC AGTACCAGCT 
     3481 TCAGTCTTTC AAACCTCACA CTGCCCACCA AAGGTGCCCG AGAGAAGGCC ACGCCCTTCC 
     3541 CCAGTCTGAA AGGAAACAGG AGGGCGTTAG TGGATCAGAA GTCATCTGTC ATTAAACACA 
     3601 GCCCAACAGT GAAAAGAGAA CCTCCATCAC CCCAGGGTCG ATCCAGCAAT TCTAGTGAGA 
     3661 ACCAGCAGTT CCTGAAGGAG GTGGTGCACA GCGTGCTGGA CGGCCAGGGA GTTGGCTGGC 
     3721 TCAACATGAA AAAGGTGCGC CGGCTGCTGG AGAGCGAGCA GCTGCGAGTC TTTGTCCTGA 
     3781 GCAAGCTGAA CCGCATGGTG CAGTCAGAGG ACGATGCCCG GCAGGACATC ATCCCGGATG 
     3841 TGGAGATCAG TCGGAAGGTG TACAAGGGAA TGTTAGACCT CCTCAAGTGT ACAGTCCTCA 
     3901 GCTTGGAGCA GTCCTATGCC CACGCGGGTC TGGGTGGCAT GGCCAGCATC TTTGGGCTTT 
     3961 TGGAGATTGC CCAGACCCAC TACTATAGTA AAGAACCAGA CAAGCGGAAG AGAAGTCCAA 
     4021 CAGAAAGTGT AAATACCCCA GTTGGCAAGG ATCCTGGCCT AGCTGGGCGG GGGGACCCAA 
     4081 AGGCTATGGC ACAACTGAGA GTTCCACAAC TGGGACCTCG GGCACCAAGT GCCACAGGAA 
     4141 AGGGTCCTAA GGAACTGGAC ACCAGAAGTT TAAAGGAAGA AAATTTTATA GCATCTATTG 
     4201 GGCCTGAAGT AATCAAACCT GTCTTTGACC TTGGTGAGAC AGAGGAGAAA AAGTCCCAGA 
     4261 TCAGCGCAGA CAGTGGTGTG AGCCTGACGT CTAGTTCCCA GAGGACTGAT CAAGACTCTG 
     4321 TCATCGGCGT GAGTCCAGCT GTTATGATCC GCAGCTCAAG TCAGGATTCT GAAGTTAGCA 
     4381 CCGTGGTGAG TAATAGCTCT GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG 
     4441 CAGGTGATGG ACCAGGTGGC GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT 
     4501 CTGATAGTGA AATTGAGACC AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT 
     4561 TGAAGCCAAG CATAAAGGAG AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA 
     4621 GCCAGCGAGT CTATCTCTAT GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC 
     4681 AAATGCAATT CTGGGAAGAT GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG 
     4741 GTATGGACCA GGGTCCCCAG GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC 
     4801 GGAAGCGCCT GGAAGATGAT GAAGATCGCT TGCTGGCCAC ACTTCTGCAC AACCTCATCT 
     4861 CCTACATGCT GCTGATGAAG GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA 
     4921 TGGGAAAGTC GCACATTGGG CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC 
     4981 TGGCGAACCT GAATGGACGC GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA 
     5041 AGCAGACATT TGTGGTACAT GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG 
     5101 TGTGCGATGA CTGTGTGGTG TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT 
     5161 ACGAGAAGCT CATCAACATG ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA 
     5221 GAAATGGCTC TGAGACCCAG CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT 
     5281 ACTGTGTGAA GGACAGCATG GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC 
     5341 CTGAATTGGG TGGCGAGTTC CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC 
     5401 AGGTGACCCT GGAAGGGATC AACCTCAAAT TCATGCACAA TCAGTTCCTG AAATTAAAGA 
     5461 AGTGGAGCGG ACCGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5521 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5581 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5641 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5701 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5761 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5821 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5881 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5941 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     6001 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6061 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6121 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6181 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6241 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6301 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6361 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6421 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6481 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6541 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6601 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6661 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6721 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6781 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6841 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6901 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6961 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     7021 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7081 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7141 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7201 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7261 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7321 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7381 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7441 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7501 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7561 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7621 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7681 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7741 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7801 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7861 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7921 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7981 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     8041 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8101 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8161 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8221 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8281 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8341 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8401 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8461 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8521 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8581 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8641 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8701 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8761 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8821 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8881 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8941 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     9001 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9061 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9121 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9181 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9241 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9301 ATTTAACAAA AATTTAACGC GAATTTT
//