LOCUS RC219290 8238 bp DNA circular SYN DEFINITION TRPA1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1) ACCESSION NM_007332 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4395..4486 /label="Myc-DDK" misc_feature 4516..5101 /label="PolyA signal" misc_feature 4573..4592 /label="Restriction Enzyme" misc_feature 5377..6049 /label="ColE1" misc_feature 6479..7273 /label="Kan/Neo" misc_feature 7308..7610 /label="SV40 ori" misc_feature 7696..8173 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4377..4383 /label="Restriction Enzyme" misc_feature 1020..4377 /label="TRPA1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GAAGCGCAGC CTGAGGAAGA TGTGGCGCCC TGGAGAAAAG AAGGAGCCCC 1081 AGGGCGTTGT CTATGAGGAT GTGCCGGACG ACACGGAGGA TTTCAAGGAA TCGCTTAAGG 1141 TGGTTTTTGA AGGAAGTGCA TATGGATTAC AAAACTTTAA TAAGCAAAAG AAATTAAAAA 1201 GATGTGACGA TATGGACACC TTCTTCTTGC ATTATGCTGC AGCAGAAGGC CAAATTGAGC 1261 TAATGGAGAA GATCACCAGA GATTCCTCTT TGGAAGTGCT GCATGAAATG GATGATTATG 1321 GAAATACCCC TCTGCATTGT GCTGTAGAAA AAAACCAAAT TGAAAGCGTT AAGTTTCTTC 1381 TCAGCAGAGG AGCAAACCCA AACCTCCGAA ACTTCAACAT GATGGCTCCT CTCCACATAG 1441 CTGTGCAGGG CATGAATAAT GAGGTGATGA AGGTCTTGCT TGAGCATAGA ACTATTGATG 1501 TTAATTTGGA AGGAGAAAAT GGAAACACAG CTGTGATCAT TGCGTGCACC ACAAATAATA 1561 GCGAAGCATT GCAGATTTTG CTTAACAAAG GAGCTAAGCC ATGTAAATCA AATAAATGGG 1621 GATGTTTCCC TATTCACCAA GCTGCATTTT CAGGTTCCAA AGAATGCATG GAAATAATAC 1681 TAAGGTTTGG TGAAGAGCAT GGGTACAGTA GACAGTTGCA CATTAACTTT ATGAATAATG 1741 GGAAAGCCAC CCCTCTCCAC CTGGCTGTGC AAAATGGTGA CTTGGAAATG ATCAAAATGT 1801 GCCTGGACAA TGGTGCACAA ATAGACCCAG TGGAGAAGGG AAGGTGCACA GCCATTCATT 1861 TTGCTGCCAC CCAGGGAGCC ACTGAGATTG TTAAACTGAT GATATCGTCC TATTCTGGTA 1921 GCGTGGATAT TGTTAACACA ACCGATGGAT GTCATGAGAC CATGCTTCAC AGAGCTTCAT 1981 TGTTTGATCA CCATGAGCTA GCAGACTATT TAATTTCAGT GGGAGCAGAT ATTAATAAGA 2041 TCGATTCTGA AGGACGCTCT CCACTTATAT TAGCAACTGC TTCTGCATCT TGGAATATTG 2101 TAAATTTGCT ACTCTCTAAA GGTGCCCAAG TAGACATAAA AGATAATTTT GGACGTAATT 2161 TTCTGCATTT AACTGTACAG CAACCTTATG GATTAAAAAA TCTGCGACCT GAATTTATGC 2221 AGATGCAACA GATCAAAGAG CTGGTAATGG ATGAAGACAA CGATGGGTGT ACTCCTCTAC 2281 ATTATGCATG TAGACAGGGG GGCCCTGGTT CTGTAAATAA CCTACTTGGC TTTAATGTGT 2341 CCATTCATTC CAAAAGCAAA GATAAGAAAT CACCTCTGCA TTTTGCAGCC AGTTATGGGC 2401 GTATCAATAC CTGTCAGAGG CTCCTACAAG ACATAAGTGA TACGAGGCTT CTGAATGAAG 2461 GTGACCTTCA TGGAATGACT CCTCTCCATC TGGCAGCAAA GAATGGACAT GATAAAGTAG 2521 TTCAGCTTCT TCTGAAAAAA GGTGCATTGT TTCTCAGTGA CCACAATGGC TGGACAGCTT 2581 TGCATCATGC GTCCATGGGC GGGTACACTC AGACCATGAA GGTCATTCTT GATACTAATT 2641 TGAAGTGCAC AGATCGCTTG GATGAAGACG GGAACACTGC ACTTCACTTT GCTGCAAGGG 2701 AAGGCCATGC CAAAGCCGTT GCGCTTCTTC TGAGCCACAA TGCTGACATA GTCCTGAACA 2761 AGCAGCAGGC CTCCTTTTTG CACCTTGCAC TTCACAATAA GAGGAAGGAG GTTGTTCTTA 2821 CGATCATCAG GAGCAAAAGA TGGGATGAAT GTCTTAAGAT TTTCAGTCAT AATTCTCCAG 2881 GCAATAAATG TCCAATTACA GAAATGATAG AATACCTCCC TGAATGCATG AAGGTACTTT 2941 TAGATTTCTG CATGTTGCAT TCCACAGAAG ACAAGTCCTG CCGAGACTAT TATATCGAGT 3001 ATAATTTCAA ATATCTTCAA TGTCCATTAG AATTCACCAA AAAAACACCT ACACAGGATG 3061 TTATATATGA ACCGCTTACA GCCCTCAACG CAATGGTACA AAATAACCGC ATAGAGCTTC 3121 TCAATCATCC TGTGTGTAAA GAATATTTAC TCATGAAATG GTTGGCTTAT GGATTTAGAG 3181 CTCATATGAT GAATTTAGGA TCTTACTGTC TTGGTCTCAT ACCTATGACC ATTCTCGTTG 3241 TCAATATAAA ACCAGGAATG GCTTTCAACT CAACTGGCAT CATCAATGAA ACTAGTGATC 3301 ATTCAGAAAT ACTAGATACC ACGAATTCAT ATCTAATAAA AACTTGTATG ATTTTAGTGT 3361 TTTTATCAAG TATATTTGGG TATTGCAAAG AAGCGGGGCA AATTTTCCAA CAGAAAAGGA 3421 ATTATTTTAT GGATATAAGC AATGTTCTTG AATGGATTAT CTACACGACG GGCATCATTT 3481 TTGTGCTGCC CTTGTTTGTT GAAATACCAG CTCATCTGCA GTGGCAATGT GGAGCAATTG 3541 CTGTTTACTT CTATTGGATG AATTTCTTAT TGTATCTTCA AAGATTTGAA AATTGTGGAA 3601 TTTTTATTGT TATGTTGGAG GTAATTTTGA AAACTTTGTT GAGGTCTACA GTTGTATTTA 3661 TCTTCCTTCT TCTGGCTTTT GGACTCAGCT TTTACATCCT CCTGAATTTA CAGGATCCCT 3721 TCAGCTCTCC ATTGCTTTCT ATAATCCAGA CCTTCAGCAT GATGCTAGGA GATATCAATT 3781 ATCGAGAGTC CTTCCTAGAA CCATATCTGA GAAATGAATT GGCACATCCA GTTCTGTCCT 3841 TTGCACAACT TGTTTCCTTC ACAATATTTG TCCCAATTGT CCTCATGAAT TTACTTATTG 3901 GTTTGGCAGT TGGCGACATT GCTGAGGTCC AGAAACATGC ATCATTGAAG AGGATAGCTA 3961 TGCAGGTGGA ACTTCATACC AGCTTAGAGA AGAAGCTGCC ACTTTGGTTT CTACGCAAAG 4021 TGGATCAGAA ATCCACCATC GTGTATCCCA ACAAACCCAG ATCTGGTGGG ATGTTATTCC 4081 ATATATTCTG TTTTTTATTT TGCACTGGGG AAATAAGACA AGAAATACCA AATGCTGATA 4141 AATCTTTAGA AATGGAAATA TTAAAGCAGA AATACCGGCT GAAGGATCTT ACTTTTCTCC 4201 TGGAAAAACA GCATGAGCTC ATTAAACTGA TCATTCAGAA GATGGAGATC ATCTCTGAGA 4261 CAGAGGATGA TGATAGCCAT TGTTCTTTTC AAGACAGGTT TAAGAAAGAG CAGATGGAAC 4321 AAAGGAATAG CAGATGGAAT ACTGTGTTGA GAGCAGTCAA GGCAAAAACA CACCATCTTG 4381 AGCCTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 4441 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 4501 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 4561 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 4621 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 4681 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 4741 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 4801 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 4861 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 4921 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 4981 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 5041 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 5101 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 5161 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 5221 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 5281 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 5341 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 5401 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 5461 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 5521 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 5581 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 5641 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 5701 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 5761 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 5821 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 5881 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 5941 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 6001 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 6061 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 6121 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 6181 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 6241 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 6301 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 6361 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 6421 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 6481 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 6541 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 6601 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 6661 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 6721 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 6781 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 6841 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 6901 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 6961 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 7021 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 7081 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 7141 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 7201 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 7261 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 7321 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 7381 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 7441 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 7501 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 7561 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 7621 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 7681 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 7741 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 7801 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 7861 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 7921 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 7981 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 8041 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 8101 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 8161 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 8221 AAATTTAACG CGAATTTT //