LOCUS       RC219164                7662 bp    DNA     circular  SYN 
DEFINITION  SLC8A3 (Myc-DDK-tagged)-Human solute carrier family 8 (sodium/calcium exchanger), member 3 (SLC8A3), transcript variant c
ACCESSION   NM_183002
VERSION     NM_183002.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3819..3910 
                     /label="Myc-DDK"  
     misc_feature    3940..4525 
                     /label="PolyA signal"  
     misc_feature    3997..4016 
                     /label="Restriction Enzyme"  
     misc_feature    4801..5473 
                     /label="ColE1"  
     misc_feature    5903..6697 
                     /label="Kan/Neo"  
     misc_feature    6732..7034 
                     /label="SV40 ori"  
     misc_feature    7120..7597 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3801..3807 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3801 
                     /label="SLC8A3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTGGTTA AGGTTGCAGC CTCTCACCTC TGCCTTCCTC CATTTTGGGC 
     1081 TGGTTACCTT TGTGCTCTTC CTGAATGGTC TTCGAGCAGA GGCTGGTGGC TCAGGGGACG 
     1141 TGCCAAGCAC AGGGCAGAAC AATGAGTCCT GTTCAGGGTC ATCGGACTGC AAGGAGGGTG 
     1201 TCATCCTGCC AATCTGGTAC CCGGAGAACC CTTCCCTTGG GGACAAGATT GCCAGGGTCA 
     1261 TTGTCTATTT TGTGGCCCTG ATATACATGT TCCTTGGGGT GTCCATCATT GCTGACCGCT 
     1321 TCATGGCATC TATTGAAGTC ATCACCTCTC AAGAGAGGGA GGTGACAATT AAGAAACCCA 
     1381 ATGGAGAAAC CAGCACAACC ACTATTCGGG TCTGGAATGA AACTGTCTCC AACCTGACCC 
     1441 TTATGGCCCT GGGTTCCTCT GCTCCTGAGA TACTCCTCTC TTTAATTGAG GTGTGTGGTC 
     1501 ATGGGTTCAT TGCTGGTGAT CTGGGACCTT CTACCATTGT AGGGAGTGCA GCCTTCAACA 
     1561 TGTTCATCAT CATTGGCATC TGTGTCTACG TGATCCCAGA CGGAGAGACT CGCAAGATCA 
     1621 AGCATCTACG AGTCTTCTTC ATCACCGCTG CTTGGAGTAT CTTTGCCTAC ATCTGGCTCT 
     1681 ATATGATTCT GGCAGTCTTC TCCCCTGGTG TGGTCCAGGT TTGGGAAGGC CTCCTCACTC 
     1741 TCTTCTTCTT TCCAGTGTGT GTCCTTCTGG CCTGGGTGGC AGATAAACGA CTGCTCTTCT 
     1801 ACAAATACAT GCACAAAAAG TACCGCACAG ACAAACACCG AGGAATTATC ATAGAGACAG 
     1861 AGGGTGACCA CCCTAAGGGC ATTGAGATGG ATGGGAAAAT GATGAATTCC CATTTTCTAG 
     1921 ATGGGAACCT GGTGCCCCTG GAAGGGAAGG AAGTGGATGA GTCCCGCAGA GAGATGATCC 
     1981 GGATTCTCAA GGATCTGAAG CAAAAACACC CAGAGAAGGA CTTAGATCAG CTGGTGGAGA 
     2041 TGGCCAATTA CTATGCTCTT TCCCACCAAC AGAAGAGCCG TGCCTTCTAC CGTATCCAAG 
     2101 CCACTCGTAT GATGACTGGT GCAGGCAATA TCCTGAAGAA ACATGCAGCA GAACAAGCCA 
     2161 AGAAGGCCTC CAGCATGAGC GAGGTGCACA CCGATGAGCC TGAGGACTTT ATTTCCAAGG 
     2221 TCTTCTTTGA CCCATGTTCT TACCAGTGCC TGGAGAACTG TGGGGCTGTA CTCCTGACAG 
     2281 TGGTGAGGAA AGGGGGAGAC ATGTCAAAGA CCATGTATGT GGACTACAAA ACAGAGGATG 
     2341 GTTCTGCCAA TGCAGGGGCT GACTATGAGT TCACAGAGGG CACGGTGGTT CTGAAGCCAG 
     2401 GAGAGACCCA GAAGGAGTTC TCCGTGGGCA TAATTGATGA CGACATTTTT GAGGAGGATG 
     2461 AACACTTCTT TGTAAGGTTG AGCAATGTCC GCATAGAGGA GGAGCAGCCA GAGGAGGGGA 
     2521 TGCCTCCAGC AATATTCAAC AGTCTTCCCT TGCCTCGGGC TGTCCTAGCC TCCCCTTGTG 
     2581 TGGCCACAGT TACCATCTTG GATGATGACC ATGCAGGCAT CTTCACTTTT GAATGTGATA 
     2641 CTATTCATGT CAGTGAGAGT ATTGGTGTTA TGGAGGTCAA GGTTCTGCGG ACATCAGGTG 
     2701 CCCGGGGTAC AGTCATCGTC CCCTTTAGGA CAGTAGAAGG GACAGCCAAG GGTGGCGGTG 
     2761 AGGACTTTGA AGACACATAT GGGGAGTTGG AATTCAAGAA TGATGAAACT GTGAAAACCA 
     2821 TAAGGGTTAA AATAGTAGAT GAGGAGGAAT ACGAAAGGCA AGAGAATTTC TTCATTGCCC 
     2881 TTGGTGAACC GAAATGGATG GAACGTGGAA TATCAGCGCT CCTGTTATCT CCAGATGTGA 
     2941 CAGACAGGAA GCTGACTATG GAAGAAGAGG AGGCCAAGAG GATAGCAGAG ATGGGAAAGC 
     3001 CAGTATTGGG TGAACACCCC AAACTAGAAG TCATCATTGA AGAGTCCTAT GAGTTCAAGA 
     3061 CTACGGTGGA CAAACTGATC AAGAAGACAA ACCTGGCCTT GGTTGTGGGG ACCCATTCCT 
     3121 GGAGGGACCA GTTCATGGAG GCCATCACCG TCAGTGCAGC AGGGGATGAG GATGAGGATG 
     3181 AATCCGGGGA GGAGAGGCTG CCCTCCTGCT TTGACTACGT CATGCACTTC CTGACTGTCT 
     3241 TCTGGAAGGT GCTGTTTGCC TGTGTGCCCC CCACAGAGTA CTGCCACGGC TGGGCCTGCT 
     3301 TCGCCGTCTC CATCCTCATC ATTGGCATGC TCACCGCCAT CATTGGGGAC CTGGCCTCGC 
     3361 ACTTCGGCTG CACCATTGGT CTCAAAGATT CAGTCACAGC TGTTGTTTTC GTGGCATTTG 
     3421 GCACCTCTGT CCCAGATACG TTTGCCAGCA AAGCTGCTGC CCTCCAGGAT GTATATGCAG 
     3481 ACGCCTCCAT TGGCAACGTG ACGGGCAGCA ACGCCGTCAA TGTCTTCCTG GGCATCGGCC 
     3541 TGGCCTGGTC CGTGGCCGCC ATCTACTGGG CTCTGCAGGG ACAGGAGTTC CACGTGTCGG 
     3601 CCGGCACACT GGCCTTCTCC GTCACCCTCT TCACCATCTT TGCATTTGTC TGCATCAGCG 
     3661 TGCTCTTGTA CCGAAGGCGG CCGCACCTGG GAGGGGAGCT TGGTGGCCCC CGTGGCTGCA 
     3721 AGCTCGCCAC AACATGGCTC TTTGTGAGCC TGTGGCTCCT CTACATACTC TTTGCCACAC 
     3781 TAGAGGCCTA TTGCTACATC AAGGGGTTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3841 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3901 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3961 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4021 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4081 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4141 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4201 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4261 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4321 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4381 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4441 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4501 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4561 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4621 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4681 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4741 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4801 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4861 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4921 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4981 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5041 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5101 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5161 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5221 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5281 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5341 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5401 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5461 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5521 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5581 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5641 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5701 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5761 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5821 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5881 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5941 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6001 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6061 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6121 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6181 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6241 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6301 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6361 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6421 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6481 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6541 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6601 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6661 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6721 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6781 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6841 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6901 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6961 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7021 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7081 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7141 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7201 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7261 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7321 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7381 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7441 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7501 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7561 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7621 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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