LOCUS       RC219120                8622 bp    DNA     circular  SYN 
DEFINITION  USP40 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 40 (USP40)
ACCESSION   NM_018218
VERSION     NM_018218.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4779..4870 
                     /label="Myc-DDK"  
     misc_feature    4900..5485 
                     /label="PolyA signal"  
     misc_feature    4957..4976 
                     /label="Restriction Enzyme"  
     misc_feature    5761..6433 
                     /label="ColE1"  
     misc_feature    6863..7657 
                     /label="Kan/Neo"  
     misc_feature    7692..7994 
                     /label="SV40 ori"  
     misc_feature    8080..8557 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4761..4767 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4761 
                     /label="USP40"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCACTTTTT TTAAGGGTAG TATTTAGTTT CACAATGTTT GGGGACCTGT 
     1081 TTGAAGAGGA GTATTCCACT GTGTCTAATA ATCAGTATGG AAAAGGGAAG AAATTAAAGA 
     1141 CTAAAGCTTT GGAGCCACCT GCTCCTAGAG AATTCACCAA TTTAAGCGGA ATCAGAAATC 
     1201 AGGGTGGAAC CTGTTACCTC AATTCCCTTC TTCAGACTCT TCATTTCACA CCTGAATTCA 
     1261 GAGAAGCTCT ATTTTCTCTT GGCCCAGAAG AGCTTGGTTT GTTTGAAGAT AAGGATAAAC 
     1321 CCGATGCAAA GGTTCGAATC ATCCCTTTAC AGTTACAGCG CTTGTTTGCT CAGCTTCTGC 
     1381 TCTTAGACCA GGAAGCTGCA TCCACAGCAG ACCTCACTGA CAGCTTTGGG TGGACCAGTA 
     1441 ATGAGGAAAT GAGGCAACAT GATGTGCAGG AACTGAATCG AATCCTCTTC AGCGCTTTGG 
     1501 AAACTTCTTT AGTTGGGACC TCCGGTCATG ACCTCATCTA TCGTCTGTAC CATGGAACCA 
     1561 TTGTTAACCA GATTGTTTGT AAAGAATGTA AGAACGTTAG CGAGAGGCAG GAAGACTTCT 
     1621 TAGATCTAAC AGTAGCAGTC AAAAATGTAT CCGGTTTGGA AGATGCTCTC TGGAACATGT 
     1681 ATGTAGAAGA GGAAGTTTTT GATTGTGACA ACTTGTACCA CTGTGGAACT TGTGACAGGC 
     1741 TGGTTAAAGC AGCAAAGTCG GCCAAATTAC GTAAGCTGCC TCCTTTTCTT ACTGTTTCAT 
     1801 TACTAAGATT TAATTTTGAT TTTGTGAAAT GCGAACGCTA CAAGGAAACT AGCTGTTATA 
     1861 CATTCCCTCT CCGGATTAAT CTCAAGCCCT TTTGTGAACA GAGTGAATTG GATGACTTAG 
     1921 AATATATATA TGACCTCTTC TCAGTTATTA TACACAAAGG TGGCTGCTAC GGAGGCCATT 
     1981 ACCATGTATA TATTAAAGAT GTTGATCATT TGGGAAACTG GCAGTTTCAA GAGGAAAAAA 
     2041 GTAAACCAGA TGTGAATCTG AAAGATCTCC AGAGTGAAGA AGAGATTGAT CATCCACTGA 
     2101 TGATTCTAAA AGCAATCTTA TTAGAGGAGA ATAATCTAAT TCCTGTTGAT CAGCTGGGCC 
     2161 AGAAACTTTT GAAAAAGATA GGAATATCTT GGAACAAGAA GTACAGAAAA CAGCATGGAC 
     2221 CACTGCGGAA GTTCTTACAG CTCCATTCTC AGATATTTCT ACTCAGTTCA GATGAAAGTA 
     2281 CAGTTCGTCT CTTGAAGAAT AGTTCTCTCC AGGCTGAGTC TGATTTCCAA AGGAATGACC 
     2341 AGCAAATTTT CAAGATGCTT CCTCCAGAAT CCCCAGGTTT AAACAATAGC ATCTCCTGTC 
     2401 CCCACTGGTT TGATATAAAT GATTCTAAAG TCCAGCCAAT CAGGGAAAAG GATATTGAAC 
     2461 AGCAATTTCA GGGTAAAGAA AGTGCCTACA TGTTGTTTTA TCGGAAATCC CAGTTGCAGA 
     2521 GACCCCCTGA AGCTCGAGCT AATCCAAGAT ATGGGGTTCC ATGTCATTTA CTGAATGAAA 
     2581 TGGATGCAGC TAACATTGAA CTGCAAACCA AAAGGGCAGA ATGTGATTCT GCAAACAATA 
     2641 CTTTTGAATT GCATCTTCAC CTGGGCCCTC AGTATCATTT CTTCAATGGG GCTCTGCACC 
     2701 CAGTAGTCTC TCAAACAGAA AGCGTGTGGG ATTTGACCTT TGATAAAAGA AAAACTTTAG 
     2761 GAGATCTCCG GCAGTCAATA TTTCAGCTGT TAGAATTTTG GGAAGGAGAC ATGGTTCTTA 
     2821 GTGTTGCAAA GCTTGTACCA GCAGGACTTC ACATTTACCA GTCACTTGGC GGGGATGAAC 
     2881 TGACACTGTG TGAAACTGAA ATTGCTGATG GGGAAGACAT CTTTGTGTGG AATGGGGTGG 
     2941 AGGTTGGTGG AGTCCACATT CAAACTGGTA TTGACTGCGA ACCTCTACTT TTAAATGTTC 
     3001 TTCATCTAGA CACAAGCAGT GATGGAGAAA AGTGTTGTCA GGTGATAGAA TCTCCACATG 
     3061 TCTTTCCAGC TAATGCAGAA GTGGGCACTG TCCTCACAGC CTTAGCAATC CCAGCAGGTG 
     3121 TCATCTTCAT CAACAGTGCT GGATGTCCAG GTGGGGAGGG TTGGACGGCC ATCCCCAAGG 
     3181 AAGACATGAG GAAGACGTTC AGGGAGCAAG GGCTCAGAAA TGGAAGCTCA ATTTTAATTC 
     3241 AGGATTCTCA TGATGATAAC AGCTTGTTGA CCAAGGAAGA GAAATGGGTC ACTAGTATGA 
     3301 ATGAGATTGA CTGGCTCCAC GTTAAAAATT TATGCCAGTT AGAATCTGAA GAGAAGCAAG 
     3361 TTAAAATATC AGCAACTGTT AACACAATGG TGTTTGATAT TCGAATTAAA GCCATAAAGG 
     3421 AATTAAAATT AATGAAGGAA CTAGCTGACA ACAGCTGTTT GAGACCTATT GATAGAAATG 
     3481 GGAAGCTTCT TTGTCCAGTG CCGGACAGCT ATACTTTGAA GGAAGCAGAA TTGAAGATGG 
     3541 GAAGTTCATT GGGACTGTGT CTTGGAAAAG CACCAAGTTC GTCTCAGTTG TTCCTGTTTT 
     3601 TTGCAATGGG GAGTGACGTT CAACCTGGGA CAGAAATGGA AATCGTAGTA GAAGAAACAA 
     3661 TATCTGTGAG AGATTGTTTA AAGTTAATGC TGAAGAAATC TGGCCTACAA GGAGATGCCT 
     3721 GGCATTTACG AAAAATGGAT TGGTGCTATG AAGCTGGAGA GCCTTTATGT GAAGAAGATG 
     3781 CAACACTGAA AGAACTTCTG ATATGTTCTG GAGATACTTT GCTTTTAATT GAAGGACAAC 
     3841 TTCCTCCTCT GGGTTTCCTG AAGGTGCCCA TCTGGTGGTA CCAGCTTCAG GGTCCCTCAG 
     3901 GACACTGGGA GAGTCATCAG GACCAGACCA ACTGTACTTC GTCTTGGGGC AGAGTTTGGA 
     3961 GAGCCACTTC CAGCCAAGGT GCTTCTGGGA ACGAGCCTGC GCAAGTTTCT CTCCTCTACT 
     4021 TGGGAGACAT AGAGATCTCA GAAGATGCCA CGCTGGCGGA GCTGAAGTCT CAGGCCATGA 
     4081 CCTTGCCTCC TTTCCTGGAG TTCGGTGTCC CGTCCCCAGC CCACCTCAGA GCCTGGACGG 
     4141 TGGAGAGGAA GCGCCCAGGC AGGCTTTTAC GAACTGACCG GCAGCCACTC AGGGAATATA 
     4201 AACTAGGACG GAGAATTGAG ATCTGCTTAG AGCCCCTTCA GAAAGGCGAA AACTTGGGCC 
     4261 CCCAGGACGT GCTGCTGAGG ACACAGGTGC GCATCCCTGG TGAGAGGACC TATGCCCCTG 
     4321 CCCTGGACCT GGTGTGGAAC GCGGCCCAGG GTGGGACTGC CGGCTCCCTG AGGCAGAGAG 
     4381 TTGCCGATTT CTATCGTCTT CCCGTGGAGA AGATTGAAAT TGCCAAATAC TTTCCCGAAA 
     4441 AGTTCGAGTG GCTTCCGATA TCTAGCTGGA ACCAACAAAT AACCAAGAGG AAAAAGAAAA 
     4501 AAAAACAAGA TTATTTGCAA GGGGCACCGT ATTACTTGAA AGACGGAGAT ACTATTGGTG 
     4561 TTAAGAATCT CCTGATTGAC GACGATGATG ATTTCAGTAC AATCAGAGAT GACACTGGAA 
     4621 AAGAAAAGCA GAAACAACGG GCCCTGGGGA GAAGGAAAAG CCAAGAAGCC CTCCATGAGC 
     4681 AGAGCAGCTA CATCCTCTCC AGTGCAGAGA CGCCTGCCCG GCCCCGAGCC CCGGAAACTT 
     4741 CTCTCTCCAT CCACGTGGGG AGCTTCAGAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4801 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4861 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4921 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4981 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5041 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5101 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5161 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5221 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5281 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5341 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5401 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5461 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5521 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5581 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5641 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5701 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5761 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5821 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5881 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5941 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6001 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6061 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6121 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6181 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6241 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6301 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6361 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6421 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6481 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6541 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6601 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6661 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6721 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6781 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6841 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6901 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6961 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7021 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7081 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7141 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7201 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7261 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7321 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7381 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7441 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7501 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7561 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7621 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7681 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7741 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7801 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7861 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7921 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8041 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8101 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8161 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8221 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8281 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8341 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8401 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8461 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8521 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8581 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//