LOCUS       RC219098                8808 bp    DNA     circular  SYN 
DEFINITION  MAST3 (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3)
ACCESSION   NM_015016
VERSION     NM_015016.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4965..5056 
                     /label="Myc-DDK"  
     misc_feature    5086..5671 
                     /label="PolyA signal"  
     misc_feature    5143..5162 
                     /label="Restriction Enzyme"  
     misc_feature    5947..6619 
                     /label="ColE1"  
     misc_feature    7049..7843 
                     /label="Kan/Neo"  
     misc_feature    7878..8180 
                     /label="SV40 ori"  
     misc_feature    8266..8743 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4947..4953 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4947 
                     /label="MAST3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGAGTCG AGCCTCCTGC GGCGCCGCGG GCTCCAGAAG GAGCTGAGCC 
     1081 TGCCACGCCG AGGACGTGGC TGCCGCAGCG GGAACCGCAA GAGCTTGGTG GTAGGAACGC 
     1141 CCTCCCCGAC CCTCTCCCGG CCCCTGTCGC CATTGTCGGT CCCAACGGCA GGCAGCAGCC 
     1201 CCTTGGATAG TCCTCGGAAT TTCTCGGCTG CCTCTGCCCT AAATTTCCCC TTTGCCCGGA 
     1261 GGGCAGACGG CAGAAGATGG TCCCTCGCGT CTCTCCCATC TTCCGGCTAT GGAACCAACA 
     1321 CACCCAGCTC CACCCTCTCG TCAAGCTCAT CCTCCCGGGA ACGTCTCCAC CAGCTTCCCT 
     1381 TCCAGCCGAC GCCGGACGAG CTGCACTTCC TGTCCAAGCA CTTCCGCAGC TCAGAGAATG 
     1441 TGCTTGATGA GGAAGGCGGC CGGTCACCCC GCCTCCGACC CCGCTCTCGC AGTCTCAGCC 
     1501 CGGGCCGTGC AACGGGGACC TTCGACAATG AGATTGTCAT GATGAATCAC GTGTACCGGG 
     1561 AGAGGTTCCC CAAGGCCACA GCACAGATGG AGGGCCGTCT GCAGGAGTTC CTGACGGCCT 
     1621 ACGCGCCCGG CGCCCGGCTG GCGCTGGCTG ATGGCGTCTT GGGCTTCATC CACCACCAGA 
     1681 TCGTCGAGCT GGCCCGAGAC TGCTTGGCCA AGTCTGGCGA GAACCTCGTC ACCTCCCGCT 
     1741 ACTTCCTAGA GATGCAGGAG AAGCTGGAGC GGCTTCTGCA GGATGCCCAT GAGCGTTCGG 
     1801 ACAGTGAGGA GGTCAGCTTC ATCGTCCAGC TTGTCCGGAA ACTGCTGATC ATCATCTCAC 
     1861 GGCCAGCTCG GCTGCTGGAG TGTCTGGAGT TTGACCCTGA GGAATTTTAC CACCTGCTGG 
     1921 AGGCGGCTGA GGGCCATGCG CGGGAGGGCC AAGGCATTAA GACTGACCTT CCACAGTACA 
     1981 TCATTGGGCA GCTGGGCCTG GCCAAGGACC CCCTGGAGGA GATGGTGCCA CTGAGTCACC 
     2041 TCGAAGAAGA ACAGCCCCCA GCACCTGAGT CCCCAGAGAG CCGCGCCCTG GTCGGCCAGT 
     2101 CACGGAGGAA GCCATGCGAA AGCGACTTTG AGACCATCAA ACTCATTAGC AACGGAGCCT 
     2161 ATGGGGCCGT CTACCTGGTG CGGCACCGTG ACACACGGCA GCGCTTTGCC ATCAAGAAGA 
     2221 TCAACAAACA GAACTTGATC CTGCGTAACC AGATCCAGCA GGTCTTTGTG GAGCGTGACA 
     2281 TTCTCACCTT TGCCGAGAAC CCCTTTGTGG TCAGCATGTT CTGCTCCTTT GAGACCCGGC 
     2341 GCCACCTATG TATGGTCATG GAATACGTGG AAGGCGGCGA CTGCGCCACG CTCCTGAAGA 
     2401 ACATGGGCCC GCTGCCCGTG GACATGGCCC GCCTGTACTT CGCCGAGACG GTGTTGGCGC 
     2461 TGGAGTACCT GCATAACTAT GGCATCGTGC ACCGTGACCT CAAACCAGAC AATCTGCTCA 
     2521 TCACCTCGCT TGGCCACATC AAGCTCACGG ACTTCGGCCT GTCCAAGATC GGCCTCATGA 
     2581 GCATGGCCAC CAACCTCTAT GAGGGCCACA TCGAGAAGGA CGCCCGAGAG TTCATCGACA 
     2641 AGCAGGTGTG TGGGACGCCG GAGTACATAG CCCCCGAGGT GATCTTCCGC CAGGGCTATG 
     2701 GGAAGCCAGT GGACTGGTGG GCCATGGGCG TCGTCCTCTA TGAGTTTCTG GTGGGCTGCG 
     2761 TGCCTTTCTT TGGAGATACC CCCGAGGAAC TCTTCGGTCA GGTGGTCAGC GATGAGATCA 
     2821 TGTGGCCAGA GGGAGATGAG GCCCTTCCAG CAGACGCCCA GGACCTCATC ACCAGGTTGC 
     2881 TCCGGCAGAG CCCGCTGGAC CGTCTGGGCA CTGGTGGCAC CCACGAAGTG AAGCAGCACC 
     2941 CCTTTTTCCT GGCCCTGGAC TGGGCAGGGC TTCTCCGACA CAAAGCCGAG TTCGTGCCCC 
     3001 AGCTCGAAGC TGAGGATGAT ACCAGCTACT TTGACACACG TTCGGAACGT TACCGCCATC 
     3061 TGGGCTCCGA GGACGACGAG ACCAATGATG AAGAATCGTC CACAGAGATC CCCCAGTTCT 
     3121 CCTCCTGCTC CCACCGGTTC AGCAAGGTCT ACAGCAGCTC TGAGTTCCTG GCCGTCCAGC 
     3181 CCACTCCTAC CTTCGCTGAA AGGAGCTTCA GTGAAGACCG GGAGGAGGGG TGGGAGCGCA 
     3241 GCGAAGTGGA CTATGGCCGC CGGCTGAGTG CTGACATCCG GCTGAGGTCC TGGACATCCT 
     3301 CTGGATCCTC CTGTCAGTCA TCTTCGTCCC AGCCCGAGCG GGGTCCCAGC CCATCTCTCC 
     3361 TGAATACCAT CAGCCTGGAC ACAATGCCCA AGTTTGCCTT CTCATCAGAG GATGAGGGGG 
     3421 TAGGCCCAGG CCCTGCAGGC CCCAAGAGGC CCGTCTTCAT TCTAGGGGAG CCTGACCCCC 
     3481 CACCAGCGGC CACCCCAGTG ATGCCCAAGC CCTCGAGCCT TTCTGCCGAC ACAGCTGCTC 
     3541 TCAGCCACGC CCGCCTACGG AGCAATAGCA TCGGCGCCCG ACACTCCACA CCAAGGCCTC 
     3601 TGGATGCCGG CCGGGGCCGC CGCCTTGGGG GCCCAAGAGA CCCAGCCCCT GAGAAGTCCA 
     3661 GAGCCTCCTC CAGCGGTGGC AGTGGTGGCG GCAGTGGGGG CCGCGTGCCC AAGTCAGCCT 
     3721 CTGTCTCTGC CCTGTCCCTC ATCATCACGG CAGATGATGG CAGCGGCGGC CCCCTCATGA 
     3781 GCCCCCTTTC CCCGCGCTCT CTGTCCTCGA ACCCGTCGTC CCGTGACTCT TCGCCGAGCC 
     3841 GAGACCCGTC CCCCGTGTGT GGCAGCCTGC GGCCCCCCAT CGTTATCCAC AGCTCTGGCA 
     3901 AGAAGTACGG CTTCAGCCTG CGGGCGATCC GCGTCTACAT GGGTGATAGC GACGTCTACA 
     3961 CTGTGCACCA CGTCGTCTGG AGTGTGGAGG ACGGAAGCCC CGCCCAGGAG GCGGGCCTGC 
     4021 GGGCTGGGGA CCTCATCACC CACATCAACG GGGAGTCAGT GCTGGGGCTG GTGCACATGG 
     4081 ACGTCGTGGA GCTGCTGCTG AAGAGCGGCA ACAAGATATC CCTGCGGACC ACAGCCCTGG 
     4141 AGAACACCTC CATCAAGGTG GGCCCCGCCC GGAAGAATGT GGCCAAGGGC CGCATGGCAC 
     4201 GCAGGAGCAA GAGGAGCCGT CGGCGGGAGA CCCAGGATCG GCGGAAGTCA CTTTTCAAGA 
     4261 AGATCTCCAA GCAGACCTCC GTGCTGCACA CCAGCCGCAG CTTCTCCTCC GGACTCCACC 
     4321 ACTCACTGTC ATCCAGTGAG AGCCTCCCCG GCTCGCCCAC CCACAGCCTC TCCCCCAGCC 
     4381 CCACCACTCC CTGCCGAAGC CCAGCCCCTG ATGTCCCAGC AGATACCACT GCATCCCCAC 
     4441 CCAGCGCATC CCCGAGCTCC AGCAGCCCCG CCTCCCCAGC TGCTGCTGGC CACACCCGCC 
     4501 CCAGCTCCCT GCACGGCCTG GCTGCCAAGC TTGGGCCACC CCGCCCCAAG ACTGGCCGCC 
     4561 GCAAGTCCAC CAGCAGCATC CCGCCCTCCC CGCTGGCCTG CCCGCCCATC TCCGCGCCCC 
     4621 CACCCCGCTC GCCCTCGCCC CTGCCCGGGC ACCCGCCCGC ACCTGCCCGA TCCCCGCGGC 
     4681 TGCGCCGGGG CCAGTCAGCT GACAAGCTGG GCACAGGGGA GCGGCTGGAT GGGGAGGCGG 
     4741 GGCGGCGCAC TCGTGGGCCA GAGGCCGAGC TCGTGGTCAT GCGGCGGCTG CACCTGTCCG 
     4801 AGCGCCGAGA CTCCTTCAAG AAGCAGGAGG CCGTGCAGGA GGTTAGCTTC GATGAGCCGC 
     4861 AGGAGGAGGC CACTGGGCTG CCCACCTCAG TGCCACAGAT CGCCGTGGAG GGCGAGGAAG 
     4921 CCGTGCCAGT AGCTCTCGGG CCCACCGGAA GAGACACGCG TACGCGGCCG CTCGAGCAGA 
     4981 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5041 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5101 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5161 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5221 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5281 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5341 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5401 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5461 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5521 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5581 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5641 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5701 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5761 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5821 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5881 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5941 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6001 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6061 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6121 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6181 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6241 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6301 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6361 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6421 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6481 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6541 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6601 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6661 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6721 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6781 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6841 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6901 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6961 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7021 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7081 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7141 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7201 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7261 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7321 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7381 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7441 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7501 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7561 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7621 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7681 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7741 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7801 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7861 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7921 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7981 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8041 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8101 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8161 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8221 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8281 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8341 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8401 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8461 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8521 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8581 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8641 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8701 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8761 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//