LOCUS       RC219084                8475 bp    DNA     circular  SYN 
DEFINITION  SORCS1 (Myc-DDK-tagged)-Human sortilin-related VPS10 domain containing receptor 1 (SORCS1), transcript variant 2
ACCESSION   NM_001013031
VERSION     NM_001013031.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4632..4723 
                     /label="Myc-DDK"  
     misc_feature    4753..5338 
                     /label="PolyA signal"  
     misc_feature    4810..4829 
                     /label="Restriction Enzyme"  
     misc_feature    5614..6286 
                     /label="ColE1"  
     misc_feature    6716..7510 
                     /label="Kan/Neo"  
     misc_feature    7545..7847 
                     /label="SV40 ori"  
     misc_feature    7933..8410 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4614..4620 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4614 
                     /label="SORCS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAAAAGTT GGCGCCGGCG GCGGCTCCCA AGCCCGGCTG AGCGCGCTCC 
     1081 TCGCCGGCGC GGGGCTCTTG ATCCTCTGCG CCCCGGGCGT CTGCGGCGGC GGCTCCTGCT 
     1141 GCCCCTCGCC GCACCCCAGC TCCGCTCCAC GCTCGGCCTC GACCCCTAGG GGCTTTTCCC 
     1201 ACCAGGGGCG GCCAGGCAGG GCTCCTGCCA CGCCCCTGCC CCTCGTAGTG CGTCCCCTGT 
     1261 TCTCAGTGGC CCCCGGGGAC CGAGCGCTAT CCCTGGAGCG GGCTCGGGGC ACTGGGGCAT 
     1321 CCATGGCGGT TGCTGCACGC TCCGGCCGGA GGAGACGGAG CGGAGCGGAT CAGGAGAAGG 
     1381 CAGAACGGGG AGAGGGCGCG AGTCGGAGCC CCCGGGGAGT GCTAAGAGAT GGAGGGCAGC 
     1441 AGGAGCCTGG GACTCGGGAG CGGGACCCGG ACAAAGCCAC CCGCTTCCGG ATGGAGGAGC 
     1501 TGAGACTGAC CAGCACCACG TTTGCGCTGA CGGGAGACTC AGCACACAAC CAAGCCATGG 
     1561 TCCACTGGTC TGGCCACAAC AGCAGCGTGA TTCTCATTTT GACAAAGCTC TATGACTATA 
     1621 ACCTGGGGAG CATCACAGAG AGCTCGCTTT GGAGGTCAAC CGATTATGGA ACAACCTATG 
     1681 AGAAGCTGAA TGATAAAGTT GGTTTGAAAA CCATTTTGAG CTATCTCTAT GTGTGTCCTA 
     1741 CCAACAAGCG TAAGATAATG TTACTCACAG ACCCGGAGAT TGAGAGCAGT TTATTGATCA 
     1801 GCTCAGATGA AGGGGCAACT TATCAAAAGT ACCGGCTGAA CTTCTACATT CAAAGCTTGC 
     1861 TTTTTCACCC CAAACAAGAA GACTGGATTC TGGCATACAG TCAAGACCAA AAGTTATACA 
     1921 GCTCTGCTGA ATTTGGGAGA AGATGGCAGC TTATCCAAGA AGGGGTTGTA CCAAACAGGT 
     1981 TCTACTGGTC TGTGATGGGG TCAAATAAAG AACCAGACCT TGTGCATCTT GAGGCCAGAA 
     2041 CTGTGGATGG TCATTCACAT TATCTAACTT GCCGAATGCA GAACTGTACA GAGGCCAACA 
     2101 GGAATCAGCC TTTTCCAGGC TACATTGACC CAGACTCTTT GATTGTTCAG GATCATTATG 
     2161 TGTTTGTTCA GCTGACATCA GGAGGGCGGC CACATTACTA CGTGTCCTAC CGAAGGAATG 
     2221 CATTTGCCCA AATGAAGCTT CCGAAATATG CTTTGCCCAA GGACATGCAT GTTATCAGCA 
     2281 CCGATGAGAA TCAGGTGTTC GCAGCGGTCC AAGAATGGAA CCAGAATGAC ACGTACAACC 
     2341 TCTACATCTC AGACACACGT GGTGTCTACT TCACCCTGGC CTTGGAGAAT GTCCAGAGCA 
     2401 GCAGAGGCCC TGAGGGCAAC ATCATGATCG ACCTCTATGA GGTAGCAGGG ATAAAGGGAA 
     2461 TGTTCTTGGC TAACAAGAAG ATTGACAACC AAGTGAAGAC TTTCATCACA TATAACAAAG 
     2521 GCAGAGACTG GCGTTTGCTG CAGGCGCCGG ACACGGATCT AAGGGGGGAC CCCGTGCACT 
     2581 GCTTGCTGCC CTATTGCTCA CTACACCTTC ACCTGAAGGT CTCTGAGAAT CCCTACACAT 
     2641 CAGGGATCAT TGCCAGCAAA GACACAGCTC CAAGCATCAT AGTGGCATCA GGTAATATAG 
     2701 GTTCTGAATT GTCAGACACT GACATCAGCA TGTTTGTCTC TTCAGATGCA GGGAACACCT 
     2761 GGAGACAGAT CTTTGAAGAA GAGCACAGTG TTTTGTACCT GGATCAAGGT GGAGTCCTGG 
     2821 TTGCTATGAA ACACACATCT CTCCCAATTC GACATCTTTG GTTGAGTTTT GATGAAGGGA 
     2881 GATCTTGGAG CAAATACAGT TTCACATCTA TTCCACTTTT TGTGGATGGG GTTCTGGGTG 
     2941 AGCCTGGAGA AGAGACTCTC ATCATGACAG TGTTTGGACA CTTCAGCCAC CGCTCTGAAT 
     3001 GGCAGCTGGT CAAAGTAGAT TACAAGTCCA TTTTTGATAG ACGGTGTGCC GAAGAGGACT 
     3061 ACAGACCTTG GCAGCTGCAC AGCCAGGGGG AAGCATGTAT CATGGGAGCA AAAAGGATAT 
     3121 ATAAGAAGCG AAAATCAGAG CGGAAGTGTA TGCAAGGAAA ATATGCAGGA GCTATGGAAT 
     3181 CTGAACCCTG TGTCTGCACT GAGGCTGATT TTGATTGCGA CTATGGTTAT GAGCGACACA 
     3241 GCAATGGCCA GTGCCTGCCG GCATTTTGGT TCAATCCATC CTCTCTGTCA AAGGATTGCA 
     3301 GCTTGGGACA GAGTTACCTC AATAGTACTG GGTACAGGAA GGTGGTTTCC AATAATTGCA 
     3361 CTGATGGCGT AAGGGAACAG TACACTGCCA AACCGCAGAA GTGCCCAGGG AAAGCCCCGC 
     3421 GGGGGCTGCG GATAGTCACG GCTGATGGAA AGCTGACAGC GGAACAAGGA CACAACGTCA 
     3481 CTCTCATGGT GCAATTAGAA GAGGGTGATG TTCAGCGGAC ACTCATCCAA GTGGACTTTG 
     3541 GCGATGGTAT CGCGGTGTCT TACGTCAATC TCAGCTCCAT GGAAGATGGG ATCAAACACG 
     3601 TCTATCAGAA CGTGGGCATT TTCCGTGTGA CCGTGCAGGT GGACAACAGT CTGGGTTCTG 
     3661 ACAGCGCCGT CCTGTACTTA CATGTAACTT GTCCCTTGGA GCACGTGCAC CTGTCTCTTC 
     3721 CCTTTGTCAC CACAAAGAAC AAAGAGGTCA ATGCGACGGC AGTGCTGTGG CCCAGCCAAG 
     3781 TGGGCACCCT CACTTACGTG TGGTGGTACG GAAACAACAC GGAGCCTTTG ATCACCTTGG 
     3841 AGGGAAGCAT ATCCTTCAGA TTTACTTCAG AAGGAATGAA TACCATCACA GTGCAGGTCT 
     3901 CAGCTGGGAA TGCCATCCTA CAAGACACAA AGACCATCGC AGTATATGAG GAATTCCGGT 
     3961 CTCTTCGCTT GTCCTTTTCT CCAAACCTGG ATGACTACAA CCCGGACATC CCTGAGTGGA 
     4021 GGAGGGACAT CGGTCGAGTC ATCAAAAAAT CCCTGGTGGA AGCCACAGGG GTTCCAGGCC 
     4081 AGCACATCCT GGTGGCGGTG CTCCCTGGCT TACCCACCAC TGCTGAACTC TTTGTCCTAC 
     4141 CCTATCAGGA TCCAGCTGGA GAAAACAAAA GGTCAACTGA TGACCTGGAG CAGATATCAG 
     4201 AATTGCTGAT CCACACGCTC AACCAAAACT CAGTACACTT CGAGCTGAAG CCAGGAGTCC 
     4261 GAGTCCTTGT CCATGCTGCT CACTTAACAG CGGCCCCCCT GGTGGACCTC ACTCCAACCC 
     4321 ACAGTGGATC TGCCATGCTG ATGCTGCTCT CAGTGGTGTT TGTGGGGCTG GCAGTGTTCG 
     4381 TCATCTACAA GTTTAAAAGG AAGATCCCGG GGATTAATGT TTATGCCCAG ATGCAGAATG 
     4441 AGAAAGAACA AGAGATGATC AGTCCAGTCA GTCACTCTGA AAGCAGGCCC AATGTCCCTC 
     4501 AGACTGAACT AAGGAGGCCT GGCCAGCTTA TAGATGAGAA GGTGGAATCC CAGCTCATAG 
     4561 GAAGTATTTC CATAGTTGCT GAGAATCAAA GCACAAAAGA AATCCCTACC TATGTAAATG 
     4621 TTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4681 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4741 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4801 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4861 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4921 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4981 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5041 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5101 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5161 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5221 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5281 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5341 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5461 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5521 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5581 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5641 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5761 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5821 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5881 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5941 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6061 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6121 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6181 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6241 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6301 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6361 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6421 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6481 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6541 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6601 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6661 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6721 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6781 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6841 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6901 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6961 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7021 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7081 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7141 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7201 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7261 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7321 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7381 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7441 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7501 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7561 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7621 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7681 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7801 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7861 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7921 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7981 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8041 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8101 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8161 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8221 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8281 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8341 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8401 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8461 TTTAACGCGA ATTTT
//