LOCUS       RC219080                8754 bp    DNA     circular  SYN 
DEFINITION  OPLAH (Myc-DDK-tagged)-Human 5-oxoprolinase (ATP-hydrolysing) (OPLAH)
ACCESSION   NM_017570
VERSION     NM_017570.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4909..5000 
                     /label="Myc-DDK"  
     misc_feature    5030..5615 
                     /label="PolyA signal"  
     misc_feature    5087..5106 
                     /label="Restriction Enzyme"  
     misc_feature    5891..6563 
                     /label="ColE1"  
     misc_feature    6993..7787 
                     /label="Kan/Neo"  
     misc_feature    7822..8124 
                     /label="SV40 ori"  
     misc_feature    8210..8687 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4884..4891 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4884 
                     /label="OPLAH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGCCCC GAGGGCCGCT TCCACTTTGC CATCGACCGT GGGGGTACCT 
     1081 TCACAGACGT CTTTGCCCAG TGCCCAGGGG GGCACGTGCG GGTCTTAAAA CTGCTCTCAG 
     1141 AGGACCCTGC CAACTATGCG GACGCGCCAA CCGAAGGCAT CCGCCGCATC CTGGAGCAGG 
     1201 AGGCCGGCAT GCTCCTGCCC CGGGACCAGC CGCTGGACTC CAGTCATATC GCCAGCATCC 
     1261 GCATGGGCAC CACAGTGGCC ACCAACGCAC TGCTGGAGCG GAAGGGGGAG CGGGTGGCGC 
     1321 TGCTGGTGAC ACGTGGCTTC CGAGACCTGC TGCACATTGG CACCCAAGCC CGTGGGGACC 
     1381 TCTTTGACCT GGCCGTGCCC ATGCCTGAGG TGCTGTATGA AGAGGTGCTG GAGGTGGACG 
     1441 AACGCGTGGT GCTGCACCGT GGAGAGGCGG GCACCGGGAC GCCTGTGAAA GGCCGCACGG 
     1501 GGGACCTGCT GGAAGTGCAG CAGCCTGTGG ACCTGGGGGC CCTGCGTGGG AAGCTGGAGG 
     1561 GGCTGCTATC TCGAGGCATC CGCAGCCTGG CTGTGGTGCT CATGCACTCG TACACGTGGG 
     1621 CCCAGCATGA GCAGCAGGTG GGTGTGCTGG CCCGGGAGCT GGGCTTCACG CACGTGTCAC 
     1681 TGTCCTCGGA GGCCATGCCC ATGGTGCGCA TCGTCCCTCG GGGGCACACG GCCTGTGCCG 
     1741 ACGCCTACCT CACGCCCGCC ATCCAGCGCT ACGTGCAGGG CTTCTGCCGT GGCTTCCAGG 
     1801 GCCAACTCAA GGATGTGCAG GTGTTGTTCA TGCGCTCCGA TGGCGGCCTG GCGCCCATGG 
     1861 ACACCTTCAG CGGCTCCAGT GCTGTGCTCT CGGGCCCGGC CGGCGGCGTG GTGGGCTACT 
     1921 CAGCCACCAC CTACCAGCAG GAGGGTGGCC AGCCTGTCAT CGGCTTTGAC ATGGGAGGCA 
     1981 CGTCCACGGA TGTGAGCCGC TATGCTGGGG AATTCGAGCA CGTCTTCGAG GCCAGCACAG 
     2041 CTGGCGTCAC CCTCCAGGCC CCGCAGCTGG ACATCAACAC CGTGGCAGCG GGAGGGGGTT 
     2101 CCCGCCTCTT CTTCAGGTCT GGCCTCTTTG TGGTTGGGCC CGAGTCAGCA GGAGCCCACC 
     2161 CAGGACCCGC CTGCTACCGC AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC 
     2221 TGGGTCGCCT GCTGCCTGCC TCCTTCCCCT GCATTTTTGG GCCGGGAGAG AACCAACCAC 
     2281 TTTCCCCTGA GGCCTCCCGC AAAGCCCTGG AGGCTGTGGC CACTGAGGTC AACAGCTTCC 
     2341 TGACCAACGG GCCCTGCCCG GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTCG 
     2401 TGCGCGTGGC CAACGAGGCC ATGTGCCGGC CCATCCGTGC ACTCACGCAG GCAAGAGGCC 
     2461 ATGACCCCTC AGCCCATGTG CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCATGTG 
     2521 CCATCGCCCG GGCCCTGGGC ATGGACACGG TGCACATCCA CAGGCACAGT GGGCTGCTGT 
     2581 CGGCCCTGGG GCTGGCCCTG GCTGACGTGG TGCATGAGGC ACAGGAACCC TGCTCCCTGC 
     2641 TCTACGCGCC TGAGACCTTC GTGCAGCTGG ACCAGAGGCT GAGCCGCCTG GAGGAGCAGT 
     2701 GTGTGGATGC TCTGCAGGCC CAGGGCTTCC CCAGGTCCCA GATCAGCACT GAGAGCTTCC 
     2761 TGCACCTGCG CTACCAGGGC ACGGACTGTG CTCTGATGGT GTCTGCCCAC CAGCACCCAG 
     2821 CCACAGCCCG CTCGCCCCGT GCGGGGGACT TCGGGGCAGC CTTTGTGGAG CGGTACATGA 
     2881 GGGAGTTTGG CTTTGTCATA CCTGAGCGGC CGGTGGTCGT GGACGATGTG CGAGTGCGGG 
     2941 GCACCGGCCG CAGTGGTCTT CGCCTCGAGG ATGCCCCCAA AGCCCAGACC GGGCCTCCCC 
     3001 GGGTGGACAA GATGACCCAG TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC 
     3061 TGCTGGCAGA GCTGGGCTAT GGGCACAAGC TCCATGGGCC CTGCCTCATC ATCGACAGTA 
     3121 ACAGCACCAT CCTGGTGGAG CCAGGTTGCC AGGCAGAGGT GACCAAGACA GGGGACATCT 
     3181 GCATCTCCGT GGGGGCCGAA GTCCCCGGCA CAGTGGGCCC CCAGCTGGAC CCTATCCAGC 
     3241 TGTCCATCTT CTCACACCGC TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTGCAGC 
     3301 GCACAGCCAT CTCCACCAAC ATCAAGGAGC GTCTGGACTT CTCCTGTGCC CTCTTTGGGC 
     3361 CCGATGGGGG GCTGGTGTCC AATGCCCCCC ACATCCCTGT GCACCTGGGT GCCATGCAGG 
     3421 AGACGGTGCA GTTCCAGATT CAGCACCTGG GGGCCGATCT CCACCCTGGC GACGTGCTAC 
     3481 TGAGCAACCA TCCCAGTGCC GGGGGCAGCC ACCTGCCAGA CCTGACTGTT ATCACACCGG 
     3541 TGTTTTGGCC GGGTCAGACG CGGCCTGTGT TCTATGTGGC CAGCCGAGGG CACCACGCAG 
     3601 ACATCGGGGG CATCACACCA GGCTCCATGC CCCCCCACTC CACCATGCTG CAACAGGAGG 
     3661 GTGCCGTCTT TCTGTCCTTC AAACTTGTCC AGGGGGGCGT CTTCCAGGAG GAGGCGGTGA 
     3721 CGGAGGCCCT GCGGGCGCCA GGCAAGGTCC CCAACTGCAG CGGAACCAGA AACCTGCACG 
     3781 ACAACCTGTC GGACCTCCGT GCCCAGGTGG CAGCCAACCA GAAGGGCATC CAGCTGGTGG 
     3841 GGGAGCTCAT TGGGCAGTAC GGCCTGGACG TGGTGCAGGC CTACATGGGC CATATTCAGG 
     3901 CAAACGCTGA GCTGGCCGTG CGAGACATGT TGCGTGCCTT TGGAACCTCC CGGCAGGCCC 
     3961 GGGGCCTGCC CCTGGAGGTG TCCTCGGAAG ACCACATGGA CGACGGTTCC CCCATCCGCC 
     4021 TCCGTGTGCA GATCAGCCTG AGTCAGGGCA GCGCCGTGTT TGACTTCAGC GGCACTGGGC 
     4081 CGGAGGTGTT TGGTAATCTC AACGCACCGC GGGCCGTAAC CCTGTCCGCC CTCATCTACT 
     4141 GCCTGCGCTG TCTGGTGGGC CGCGACATCC CACTCAACCA GGGCTGCCTG GCGCCAGTGC 
     4201 GCGTGGTCAT TCCCCGAGGC TCCATCCTGG ACCCGTCGCC CGAGGCGGCG GTGGTGGGCG 
     4261 GCAACGTGCT CACGTCGCAG CGCGTGGTGG ATGTCATCCT GGGGGCCTTT GGGGCCTGCG 
     4321 CCGCCTCCCA GGGCTGCATG AACAACGTGA CCCTGGGCAA CGCCCACATG GGCTACTACG 
     4381 AGACGGTGGC GGGCGGCGCG GGCGCGGGTC CCAGCTGGCA CGGGCGCAGC GGTGTGCACA 
     4441 GCCACATGAC CAACACACGC ATCACCGACC CTGAGATCCT GGAGAGCCGG TACCCGGTCA 
     4501 TCCTGCGCCG CTTCGAGCTG CGGCGGGGCT CGGGGGGCAG AGGCCGCTTC CGAGGCGGCG 
     4561 ACGGCGTCAC CCGCGAGCTG CTCTTTCGTG AGGAGGCGCT GCTGTCAGTG CTGACCGAGC 
     4621 GCCGCGCCTT CCGGCCATAC GGGCTCCACG GGGGCGAGCC TGGCGCCCGC GGCCTAAACC 
     4681 TGCTGATCCG CAAAAACGGC CGGACGGTGA ATCTGGGCGG CAAGACGTCG GTGACCGTGT 
     4741 ACCCCGGGGA TGTGTTCTGT CTCCACACGC CCGGCGGCGG TGGCTATGGG GACCCGGAGG 
     4801 ACCCCGCCCC ACCGCCGGGG TCGCCCCCGC AAGCACTGGC CTTTCCCGAG CACGGCAGCG 
     4861 TCTATGAGTA TCGCCGGGCC CAGGAGGCCG TGAGCGGACC GACGCGTACG CGGCCGCTCG 
     4921 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4981 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5041 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5101 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5161 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5221 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5281 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5341 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5401 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5461 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5521 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5581 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5641 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5701 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5761 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5821 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5881 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5941 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6001 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6061 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6121 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6181 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6241 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6301 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6361 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6421 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6481 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6541 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6601 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6661 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6721 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6781 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6841 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6901 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6961 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7021 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7081 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7141 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7201 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7261 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7321 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7381 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7441 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7501 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7561 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7621 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7681 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7741 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7801 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7861 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7921 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7981 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8041 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8101 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8161 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8221 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8281 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8341 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8401 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8461 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8521 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8581 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8641 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8701 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//