LOCUS       RC219024L1                9579 bp    DNA     circular  SYN 
DEFINITION  UBE3B (Myc-DDK-tagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 1
ACCESSION   NM_130466
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7870..8050 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8778..9437 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8209..8777 
                     /label="ColE1"  
     misc_feature    6745..6765 
                     /label="LR50 primer"  
     misc_feature    6542..6613 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6754..7345 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6524..6530 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6524 
                     /label="UBE3B"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTTCACCCTG TCTCAGACCT CGAGAGCATG 
     3361 GTTCATCGAT AGAGCCCGTC AGGCACGAGA AGAAAGGCTT GTGCAGAAGG AACGGGAGCG 
     3421 GGCAGCTGTT GTGATCCAGG CCCATGTCCG GAGTTTTCTC TGTCGGAGTC GACTGCAGAG 
     3481 AGATATCAGG AGAGAGATTG ATGACTTTTT TAAAGCAGAT GACCCTGAGT CCACTAAAAG 
     3541 AAGTGCACTT TGTATTTTCA AGATTGCCAG GAAACTGCTG TTCCTATTCA GAATCAAAGA 
     3601 GGATAATGAG AGATTTGAGA AGTTGTGTCG CAGCATCCTG AGCAGCATGG ATGCTGAGAA 
     3661 TGAGCCTAAG GTGTGGTATG TGTCCCTGGC TTGTTCTAAG GACCTCACCC TCCTTTGGAT 
     3721 TCAACAGATC AAGAACATTT TGTGGTACTG CTGTGATTTT CTCAAGCAGC TCAAGCCTGA 
     3781 AATCCTGCAG GACTCCCGAC TCATCACCCT GTACCTCACG ATGCTTGTCA CCTTCACAGA 
     3841 CACTTCAACG TGGAAAATTC TTCGGGGAAA AGGTGAAAGT CTTCGACCAG CGATGAACCA 
     3901 CATTTGTGCA AATATAATGG GACATCTCAA CCAGCATGGA TTTTATTCTG TGCTGCAGAT 
     3961 ATTGTTAACC CGTGGCCTGG CAAGACCCCG TCCTTGTCTA TCCAAAGGCA CTTTAACAGC 
     4021 AGCTTTTTCT CTAGCGTTAC GCCCTGTGAT TGCTGCACAG TTCTCAGACA ATCTGATTCG 
     4081 GCCGTTCCTC ATCCACATCA TGTCTGTGCC TGCTCTGGTG ACTCATCTCA GCACAGTGAC 
     4141 CCCTGAGCGC CTCACTGTTT TAGAATCCCA TGACATGCTT CGTAAATTCA TCATATTTTT 
     4201 AAGAGACCAA GATCGATGCC GTGATGTATG TGAAAGTTTA GAAGGATGCC ATACGCTTTG 
     4261 TCTAATGGGC AACCTCCTAC ACTTGGGCTC CCTCAGCCCC AGAGTGTTAG AGGAGGAGAC 
     4321 AGATGGGTTC GTGAGTTTGC TCACCCAGAC GCTGTGCTAC TGTCAGAAGT ATGTGTCTCA 
     4381 GAAGAAGTCC AACCTGACCC ACTGGCATCC TGTCCTTGGC TGGTTCTCCC AATCTGTGGA 
     4441 TTATGGCCTT AACGAGTCAA TGCACTTGAT CACCAAACAG CTGCAGTTCT TGTGGGGGGT 
     4501 GCCTCTGATC CGGATCTTCT TCTGTGACAT CCTGAGCAAG AAGCTACTGG AGAGCCAGGA 
     4561 GCCAGCCCAC GCACAGCCAG CATCCCCTCA GAATGTGCTC CCAGTGAAGA GTCTCCTAAA 
     4621 GCGTGCTTTT CAAAAGTCGG CATCAGTCCG GAATATTCTC AGGCCTGTCG GGGGTAAACG 
     4681 GGTCGACTCT GCAGAAGTCC AGAAGGTTTG CAACATCTGT GTCCTCTACC AGACCTCGCT 
     4741 GACAACTCTC ACACAGATTC GGCTGCAGAT ACTCACAGGT CTCACTTACC TTGATGACCT 
     4801 GCTTCCCAAA CTGTGGGCAT TTATCTGTGA GCTCGGGCCC CACGGAGGGT TAAAGCTCTT 
     4861 CTTGGAATGC CTGAACAATG ACACTGAAGA GTCCAAGCAA CTCTTGGCCA TGCTGATGCT 
     4921 GTTCTGTGAC TGTTCGCGGC ACCTCATCAC AATCCTTGAT GACATTGAAG TTTATGAAGA 
     4981 ACAGATTTCA TTCAAACTGG AAGAGCTGGT CACTATCTCC TCTTTCCTGA ATTCTTTTGT 
     5041 GTTTAAGATG ATCTGGGATG GAATTGTAGA GAACGCCAAG GGTGAGACCT TGGAGCTGTT 
     5101 CCAGTCTGTC CACGGGTGGC TTATGGTGCT GTACGAGCGG GACTGCCGGC GGCGCTTCAC 
     5161 CCCCGAGGAC CACTGGCTGC GAAAGGATCT CAAACCTAGC GTGCTCTTCC AAGAACTCGA 
     5221 CAGGGACAGA AAACGGGCAC AGTTGATCCT GCAGTACATC CCACATGTCA TCCCTCACAA 
     5281 AAACAGAGTT CTACTGTTTC GAACCATGGT TACCAAGGAG AAGGAGAAAC TGGGGCTGGT 
     5341 GGAAACCAGC TCTGCCTCCC CGCATGTCAC TCACATCACC ATCCGCCGGT CCAGGATGCT 
     5401 GGAGGACGGC TACGAGCAGC TTAGGCAGCT CTCCCAGCAC GCCATGAAGG GGGTCATCCG 
     5461 TGTGAAGTTT GTCAATGACC TCGGGGTGGA CGAAGCAGGG ATTGATCAAG ACGGTGTTTT 
     5521 TAAGGAGTTC TTGGAAGAGA TCATCAAGAG AGTTTTTGAC CCAGCACTCA ATCTGTTCAA 
     5581 GACAACCAGT GGGGATGAGA GGCTGTACCC CTCACCCACA TCCTACATCC ATGAGAATTA 
     5641 CCTGCAGCTC TTCGAGTTTG TGGGGAAGAT GCTGGGGAAG GCTGTGTATG AGGGAATTGT 
     5701 GGTGGACGTG CCATTTGCAT CCTTCTTCCT GAGCCAACTG CTTGGGCACC ACCACAGCGT 
     5761 CTTCTATAGC TCGGTGGATG AACTGCCTTC TCTGGACTCC GAGTTCTATA AAAACCTCAC 
     5821 CTCCATCAAG CGCTATGATG GGGACATCAC TGACCTGGGC CTGACGCTGT CTTACGACGA 
     5881 GGACGTCATG GGTCAGCTTG TTTGCCATGA ACTGATTCCT GGAGGGAAGA CCATTCCTGT 
     5941 TACAAATGAA AATAAAATTA GCTACATCCA TCTGATGGCA CATTTTCGAA TGCACACTCA 
     6001 AATAAAAAAC CAAACAGCTG CCCTCATTAG CGGATTCCGT TCCATTATCA AACCCGAGTG 
     6061 GATCCGAATG TTCTCAACTC CTGAACTGCA GCGTCTCATC TCTGGCGACA ATGCTGAGAT 
     6121 TGATCTGGAA GATTTAAAGA AGCACACAGT CTACTACGGT GGTTTCCATG GAAGTCACAG 
     6181 AGTCATCATC TGGCTCTGGG ATATTCTGGC CTCCGACTTC ACACCGGATG AGAGAGCTAT 
     6241 GTTTCTGAAG TTCGTGACCA GCTGCTCCAG ACCCCCGCTC CTGGGATTCG CCTACCTCAA 
     6301 GCCTCCATTC TCCATCCGCT GCGTGGAGGT GTCGGACGAT CAGGACACCG GGGACACTCT 
     6361 GGGCAGCGTC CTCCGGGGCT TCTTCACCAT CCGCAAGCGG GAGCCAGGCG GCCGCCTGCC 
     6421 CACCTCCTCC ACCTGCTTCA ACCTGCTCAA GCTGCCCAAC TACAGCAAGA AGAGCGTCCT 
     6481 CCGCGAGAAG CTGCGCTACG CCATCAGCAT GAACACGGGC TTTGAACTCT CCACGCGTAC 
     6541 GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA 
     6601 TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCTGTACA AGTAGGATTC 
     6661 GTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATACA TGTTTAAGGG 
     6721 TTCCGGTTCC ACTAGGTACA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC 
     6781 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA 
     6841 TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC 
     6901 TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA 
     6961 CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC 
     7021 ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC 
     7081 ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC 
     7141 GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG 
     7201 ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT 
     7261 TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG 
     7321 AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC GTCGACCTCG ATCGAGACCT 
     7381 AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG 
     7441 GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG TACCTTTAAG 
     7501 ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT 
     7561 GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA TCTACCACAC 
     7621 ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG CCAGGGATCA GATATCCACT 
     7681 GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA 
     7741 TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG CATGGGATGG ATGACCCGGA 
     7801 GAGAGAAGTA TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACA TGGCCCGAGA 
     7861 GCTGCATCCG GACTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC 
     7921 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT 
     7981 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC 
     8041 AGTGTGGAAA ATCTCTAGCA GCATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     8101 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     8161 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     8221 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     8281 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     8341 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     8401 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     8461 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     8521 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     8581 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     8641 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     8701 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     8761 CTCACGTTAA GGGATTTTGG TCATGATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT 
     8821 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA 
     8881 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG 
     8941 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG 
     9001 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT 
     9061 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG 
     9121 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG 
     9181 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGGAA CTCCGGATGA 
     9241 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC 
     9301 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA 
     9361 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG 
     9421 GTATATCCAG TGATTTTTTT CTCCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     9481 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     9541 GGGGTCCCGC GCACATTTCC CCGAAAAGTG CCACCTGAC
//