LOCUS       RC219009                8436 bp    DNA     circular  SYN 
DEFINITION  PLCB2 (Myc-DDK-tagged)-Human phospholipase C, beta 2 (PLCB2)
ACCESSION   NM_004573
VERSION     NM_004573.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4593..4684 
                     /label="Myc-DDK"  
     misc_feature    4714..5299 
                     /label="PolyA signal"  
     misc_feature    4771..4790 
                     /label="Restriction Enzyme"  
     misc_feature    5575..6247 
                     /label="ColE1"  
     misc_feature    6677..7471 
                     /label="Kan/Neo"  
     misc_feature    7506..7808 
                     /label="SV40 ori"  
     misc_feature    7894..8371 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4575..4581 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4575 
                     /label="PLCB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCTGCTC AACCCTGTCC TGCTGCCCCC CAAGGTGAAG GCCTATCTGA 
     1081 GCCAAGGGGA GCGCTTCATC AAATGGGATG ATGAAACTAC AGTTGCCTCT CCAGTTATCC 
     1141 TCCGTGTGGA TCCTAAGGGC TACTACTTAT ACTGGACGTA TCAAAGTAAG GAGATGGAGT 
     1201 TTCTGGATAT CACCAGCATC CGGGATACTC GCTTTGGGAA GTTTGCCAAG ATGCCCAAGA 
     1261 GCCAGAAGCT CCGGGACGTC TTCAACATGG ACTTTCCTGA TAACAGTTTC CTGCTGAAGA 
     1321 CACTCACGGT GGTGTCCGGC CCGGACATGG TGGACCTCAC CTTCCACAAC TTCGTCTCCT 
     1381 ACAAGGAGAA CGTGGGCAAG GCCTGGGCTG AGGACGTACT GGCCCTAGTC AAACATCCGC 
     1441 TGACGGCCAA CGCCTCCCGC AGCACCTTCC TGGACAAGAT CCTTGTGAAG CTCAAGATGC 
     1501 AGCTCAACTC TGAAGGGAAG ATTCCGGTGA AGAACTTTTT CCAGATGTTT CCTGCTGACC 
     1561 GCAAGCGGGT GGAAGCTGCT CTCAGTGCCT GCCACCTCCC CAAAGGCAAA AATGACGCCA 
     1621 TCAATCCTGA GGACTTCCCA GAACCTGTCT ACAAGAGTTT CCTCATGAGC CTCTGTCCTC 
     1681 GGCCAGAAAT AGATGAGATC TTCACTTCTT ACCATGCTAA GGCCAAACCC TACATGACGA 
     1741 AGGAGCACCT GACCAAATTC ATCAACCAGA AACAGCGGGA CTCCCGGCTT AACTCCCTGC 
     1801 TGTTCCCGCC AGCACGGCCT GACCAGGTGC AGGGCCTCAT CGACAAGTAT GAGCCCAGTG 
     1861 GCATCAATGC ACAGAGGGGC CAGCTGTCAC CTGAAGGCAT GGTCTGGTTT CTCTGTGGGC 
     1921 CAGAGAACAG CGTGCTGGCC CAGGACAAGC TGCTGCTCCA CCACGACATG ACGCAGCCAC 
     1981 TCAATCATTA CTTCATCAAC TCGTCCCACA ACACCTACCT GACAGCCGGC CAGTTCTCAG 
     2041 GCCTCTCCTC GGCTGAGATG TACCGCCAGG TGCTGCTCTC TGGCTGCCGT TGCGTGGAGC 
     2101 TAGACTGCTG GAAGGGGAAA CCCCCTGACG AGGAGCCCAT TATCACCCAT GGCTTCACCA 
     2161 TGACCACAGA CATCTTCTTC AAAGAAGCAA TTGAGGCTAT TGCAGAAAGC GCCTTTAAGA 
     2221 CCTCCCCCTA TCCCATCATC CTGTCGTTTG AGAACCATGT GGACTCACCC CGCCAGCAGG 
     2281 CTAAGATGGC TGAGTATTGC CGGACGATCT TTGGGGATAT GCTGCTCACA GAGCCCCTGG 
     2341 AAAAGTTCCC ACTAAAACCA GGTGTCCCCC TGCCCAGCCC TGAGGATCTC AGGGGCAAGA 
     2401 TCCTCATCAA GAACAAGAAG AACCAGTTTT CTGGCCCCAC CTCCTCCAGT AAGGATACTG 
     2461 GTGGGGAGGC TGAGGGCAGC AGCCCACCCA GTGCCCCTGC AGGTGAGGGC ACAGTGTGGG 
     2521 CTGGCGAGGA AGGGACTGAG CTGGAGGAGG AGGAGGTGGA AGAGGAAGAG GAGGAGGAGT 
     2581 CAGGAAACCT GGATGAAGAA GAGATTAAGA AGATGCAGTC GGATGAGGGC ACAGCGGGCC 
     2641 TGGAAGTGAC GGCTTATGAG GAGATGTCCA GCCTAGTCAA TTACATCCAG CCCACCAAGT 
     2701 TCGTCTCCTT TGAGTTCTCT GCCCAAAAGA ACCGAAGTTA TGTCATCTCG TCCTTCACAG 
     2761 AGCTCAAGGC ATATGACCTG CTCTCCAAGG CCTCGGTGCA GTTTGTGGAC TACAACAAGC 
     2821 GCCAGATGAG CCGCATTTAC CCCAAGGGAA CCCGCATGGA CTCCTCCAAC TACATGCCCC 
     2881 AGATGTTCTG GAATGCTGGA TGCCAGATGG TTGCCCTCAA CTTCCAGACG ATGGACTTGC 
     2941 CCATGCAGCA GAACATGGCA GTATTTGAGT TCAACGGGCA GAGCGGCTAC CTCCTCAAGC 
     3001 ATGAGTTCAT GCGCCGGCCG GACAAGCAGT TCAACCCCTT CTCAGTGGAC CGCATCGACG 
     3061 TGGTGGTGGC CACCACCCTT TCCATTACGG TGATCTCTGG GCAGTTCCTG TCAGAACGCA 
     3121 GCGTGCGCAC CTATGTAGAA GTGGAGCTGT TTGGCCTTCC TGGGGACCCC AAGAGGCGCT 
     3181 ATCGAACTAA GCTGTCACCC AGTACTAACT CCATCAATCC TGTCTGGAAG GAGGAGCCCT 
     3241 TTGTCTTTGA GAAGATCTTG ATGCCTGAGC TGGCCTCCCT CAGAGTGGCT GTGATGGAGG 
     3301 AAGGCAACAA GTTTCTTGGA CACCGCATCA TCCCCATCAA TGCCCTAAAT TCTGGGTACC 
     3361 ACCACCTGTG CCTGCACAGT GAGAGCAACA TGCCCCTCAC CATGCCTGCG CTCTTCATCT 
     3421 TCCTGGAGAT GAAGGACTAC ATACCTGGTG CTTGGGCAGA TCTCACTGTG GCCCTCGCCA 
     3481 ACCCCATTAA GTTCTTCAGT GCCCATGACA CGAAGTCTGT GAAGCTCAAG GAGGCCATGG 
     3541 GAGGTCTGCC TGAGAAGCCC TTCCCACTGG CGAGTCCAGT TGCCAGCCAG GTCAATGGGG 
     3601 CGTTGGCCCC AACGAGCAAT GGGTCACCAG CAGCCAGGGC CGGGGCCAGG GAAGAGGCTA 
     3661 TGAAAGAAGC TGCGGAGCCG CGGACCGCCA GCCTGGAGGA GCTCCGGGAG CTAAAGGGCG 
     3721 TGGTGAAGCT GCAGCGGCGG CACGAGAAGG AGCTGCGAGA GTTGGAGCGG CGCGGAGCGC 
     3781 GGCGCTGGGA GGAGCTGCTG CAGCGGGGCG CGGCGCAGCT GGCGGAGCTC GGGCCACCGG 
     3841 GCGTGGGGGG CGTCGGGGCC TGCAAGCTCG GTCCCGGCAA GGGCTCTCGC AAGAAGAGGA 
     3901 GCCTGCCCCG CGAGGAGAGC GCCGGAGCCG CGCCGGGCGA GGGCCCTGAG GGCGTGGACG 
     3961 GGCGCGTGCG GGAGCTGAAA GACAGGCTGG AGCTGGAGCT GCTGCGGCAG GGCGAGGAGC 
     4021 AGTACGAGTG CGTTCTGAAG CGCAAGGAGC AGCACGTGGC CGAGCAAATC TCCAAAATGA 
     4081 TGGAGCTGGC CAGAGAGAAA CAGGCGGCAG AGCTGAAGGC CCTGAAGGAG ACGTCGGAGA 
     4141 ACGACACCAA AGAGATGAAG AAAAAGCTGG AGACAAAGAG ACTGGAGCGG ATCCAGGGCA 
     4201 TGACCAAAGT CACCACAGAC AAGATGGCCC AGGAGAGGTT GAAGAGAGAG ATTAACAACT 
     4261 CCCACATCCA GGAAGTAGTG CAGGTGATCA AGCAGATGAC GGAGAACTTG GAGAGGCACC 
     4321 AGGAGAAGCT GGAGGAGAAG CAGGCGGCTT GCCTGGAACA GATACGGGAG ATGGAAAAGC 
     4381 AGTTCCAGAA GGAGGCGCTG GCAGAGTACG AGGCCAGGAT GAAGGGTCTG GAGGCAGAGG 
     4441 TGAAGGAGTC GGTGAGGGCC TGCCTCAGGA CCTGCTTTCC CTCCGAGGCC AAGGACAAGC 
     4501 CTGAGAGGGC CTGCGAGTGC CCCCCAGAGC TGTGTGAGCA GGACCCACTC ATAGCAAAGG 
     4561 CAGATGCCCA GGAGAGCCGC CTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4621 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4681 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4741 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4801 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4861 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4921 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4981 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5041 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5101 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5161 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5221 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5281 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5341 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5401 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5461 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5521 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5581 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5641 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5701 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5761 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5821 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5881 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5941 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6001 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6061 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6121 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6181 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6241 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6301 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6361 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6421 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6481 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6541 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6601 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6661 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6721 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6781 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6841 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6901 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6961 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7021 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7081 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7141 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7201 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7261 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7321 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7381 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7441 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7501 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7561 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7621 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7681 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7801 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7861 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7921 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7981 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8041 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8101 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8161 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8221 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8281 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8341 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8401 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//