LOCUS       RC218964                9216 bp    DNA     circular  SYN 
DEFINITION  PTPRG (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, G (PTPRG)
ACCESSION   NM_002841
VERSION     NM_002841.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5373..5464 
                     /label="Myc-DDK"  
     misc_feature    5494..6079 
                     /label="PolyA signal"  
     misc_feature    5551..5570 
                     /label="Restriction Enzyme"  
     misc_feature    6355..7027 
                     /label="ColE1"  
     misc_feature    7457..8251 
                     /label="Kan/Neo"  
     misc_feature    8286..8588 
                     /label="SV40 ori"  
     misc_feature    8674..9151 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5355..5361 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5355 
                     /label="PTPRG"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGAGGTTA CTGGAACCGT GTTGGTGGAT TTTGTTCCTG AAAATCACCA 
     1081 GTTCCGTGCT CCATTATGTC GTGTGCTTCC CCGCGTTGAC AGAAGGCTAC GTTGGGGCCC 
     1141 TGCACGAGAA TAGACACGGC AGCGCAGTGC AGATCCGCAG GCGCAAGGCT TCAGGCGACC 
     1201 CGTACTGGGC CTACTCTGGT GCCTATGGTC CTGAGCACTG GGTCACGTCT AGTGTCAGCT 
     1261 GTGGGAGCCG TCACCAGTCT CCTATTGACA TTTTAGACCA GTATGCGCGT GTTGGGGAAG 
     1321 AATACCAGGA ACTGCAACTC GATGGCTTCG ACAATGAGTC TTCTAACAAA ACCTGGATGA 
     1381 AAAACACAGG GAAAACAGTC GCCATCCTTC TGAAAGACGA CTATTTTGTC AGTGGAGCTG 
     1441 GTCTACCTGG CAGATTCAAA GCTGAGAAGG TGGAATTTCA CTGGGGCCAC AGCAATGGCT 
     1501 CAGCGGGCTC TGAACACAGC ATCAATGGCA GGAGGTTTCC TGTTGAGATG CAGATTTTCT 
     1561 TTTACAATCC AGATGACTTT GACAGCTTTC AAACCGCAAT TTCTGAGAAC AGAATAATCG 
     1621 GAGCCATGGC CATATTTTTT CAAGTCAGTC CGAGGGACAA TTCTGCACTG GATCCTATTA 
     1681 TCCACGGGTT GAAGGGTGTC GTACATCATG AGAAGGAGAC CTTTCTGGAT CCTTTCGTCC 
     1741 TCCGGGACCT CCTGCCTGCA TCCCTGGGCA GCTATTATCG GTACACAGGT TCCTTGACCA 
     1801 CACCACCGTG TAGCGAAATA GTGGAGTGGA TAGTCTTCCG GAGACCCGTC CCCATCTCTT 
     1861 ACCATCAGCT TGAGGCTTTT TATTCCATCT TCACCACGGA GCAGCAAGAC CATGTCAAGT 
     1921 CGGTGGAGTA TCTGAGAAAT AACTTTCGAC CACAGCAGCG TCTGCATGAC AGGGTGGTGT 
     1981 CCAAGTCCGC CGTCCGTGAC TCCTGGAACC ACGACATGAC AGACTTCTTA GAAAACCCAC 
     2041 TGGGGACAGA AGCCTCTAAA GTTTGCAGCT CTCCACCCAT CCACATGAAG GTGCAGCCTC 
     2101 TGAACCAGAC GGCACTGCAG GTGTCCTGGA GCCAGCCGGA GACTATCTAC CACCCACCCA 
     2161 TCATGAACTA CATGATCTCC TACAGCTGGA CCAAGAATGA GGACGAGAAG GAGAAGACGT 
     2221 TTACAAAGGA CAGCGACAAA GACTTGAAAG CCACCATTAG CCATGTCTCA CCCGATAGCC 
     2281 TTTACCTGTT CCGAGTCCAG GCCGTGTGTC GGAACGACAT GCGCAGCGAC TTTAGCCAGA 
     2341 CGATGCTGTT TCAAGCTAAT ACCACTCGAA TATTCCAAGG GACCAGAATA GTGAAAACAG 
     2401 GAGTGCCCAC AGCGTCTCCT GCCTCTTCAG CCGACATGGC CCCCATCAGC TCGGGGTCTT 
     2461 CTACCTGGAC GTCCTCTGGC ATCCCATTCT CATTTGTTTC CATGGCAACT GGGATGGGCC 
     2521 CCTCCTCCAG TGGCAGCCAG GCCACAGTGG CCTCGGTGGT CACCAGCACG CTGCTCGCCG 
     2581 GCCTGGGGTT CGGCGGTGGT GGCATCTCCT CTTTCCCCAG CACTGTGTGG CCCACGCGCC 
     2641 TCCCGACGGC CGCCTCAGCC AGCAAGCAGG CGGCTAGGCC AGTCCTAGCC ACCACAGAGG 
     2701 CCTTGGCTTC TCCAGGGCCC GATGGTGATT CGTCACCAAC CAAGGACGGC GAGGGCACCG 
     2761 AGGAAGGAGA GAAGGATGAG AAAAGCGAGA GTGAGGATGG GGAGCGGGAG CACGAGGAGG 
     2821 ATGGAGAGAA GGACTCCGAA AAGAAGGAGA AGAGTGGGGT GACCCACGCT GCCGAGGAGC 
     2881 GGAATCAGAC GGAGCCCAGC CCCACACCCT CGTCTCCTAA CAGGACTGCC GAGGGAGGGC 
     2941 ATCAGACTAT ACCTGGGCAT GAGCAGGATC ACACTGCCGT CCCCACAGAC CAGACGGGCG 
     3001 GAAGGAGGGA TGCCGGCCCA GGCCTGGACC CCGACATGGT CACCTCCACC CAAGTGCCCC 
     3061 CCACCGCCAC AGAGGAGCAG TATGCAGGGA GTGATCCCAA GAGGCCCGAA ATGCCATCTA 
     3121 AAAAGCCTAT GTCCCGCGGG GACCGATTTT CTGAAGACAG CAGATTTATC ACTGTTAATC 
     3181 CAGCGGAAAA AAACACCTCT GGAATGATAA GCCGCCCTGC TCCAGGGAGG ATGGAGTGGA 
     3241 TCATCCCTCT GATTGTGGTA TCAGCCTTGA CCTTCGTGTG CCTCATCCTT CTCATTGCTG 
     3301 TGCTCGTTTA CTGGAGAGGG TGTAACAAAA TAAAGTCCAA GGGCTTTCCC AGACGTTTCC 
     3361 GTGAAGTGCC TTCTTCTGGG GAGAGAGGAG AGAAGGGGAG CAGAAAATGT TTTCAGACTG 
     3421 CTCATTTCTA TGTGGAAGAC AGCAGTTCAC CTCGAGTGGT CCCTAATGAA AGTATTCCTA 
     3481 TTATTCCTAT TCCGGATGAC ATGGAAGCCA TTCCTGTCAA ACAGTTTGTC AAACACATCG 
     3541 GTGAGCTCTA TTCTAATAAC CAGCATGGGT TCTCTGAGGA TTTTGAGGAA GTCCAGCGCT 
     3601 GTACTGCTGA TATGAACATC ACTGCAGAGC ATTCCAATCA TCCAGAAAAC AAGCACAAAA 
     3661 ACAGATACAT CAACATTTTA GCATATGATC ACAGTAGGGT GAAGTTAAGA CCTTTACCAG 
     3721 GAAAAGACTC TAAGCACAGC GACTACATTA ATGCAAACTA TGTTGATGGT TACAACAAAG 
     3781 CAAAAGCCTA CATTGCCACC CAAGGACCTT TGAAGTCTAC ATTTGAAGAT TTCTGGAGGA 
     3841 TGATTTGGGA ACAAAACACT GGAATCATTG TGATGATTAC GAACCTTGTG GAAAAAGGAA 
     3901 GACGAAAATG TGATCAGTAT TGGCCAACAG AGAACAGTGA GGAATATGGA AACATTATTG 
     3961 TCACGCTGAA GAGCACAAAA ATACATGCCT GCTACACTGT TCGTCGTTTT TCAATCAGAA 
     4021 ATACAAAAGT GAAAAAGGGT CAGAAGGGAA ATCCCAAGGG TCGTCAGAAT GAAAGGGTAG 
     4081 TGATCCAGTA TCACTATACA CAGTGGCCTG ACATGGGAGT TCCCGAGTAT GCCCTTCCAG 
     4141 TACTGACTTT CGTGAGGAGA TCCTCAGCAG CTCGGATGCC AGAAACGGGC CCTGTGTTGG 
     4201 TGCACTGCAG TGCTGGTGTG GGCAGAACAG GCACCTATAT TGTAATAGAC AGCATGCTGC 
     4261 AACAGATAAA AGACAAAAGC ACAGTTAACG TCCTGGGATT CCTGAAGCAT ATCAGGACAC 
     4321 AGCGTAACTA CCTCGTCCAG ACTGAGGAGC AGTACATTTT CATCCATGAT GCCTTGTTGG 
     4381 AAGCCATTCT TGGAAAGGAG ACTGAAGTAT CTTCAAATCA GCTGCACAGC TATGTTAACA 
     4441 GCATCCTTAT ACCAGGAGTA GGAGGAAAGA CACGACTGGA AAAGCAATTC AAGCTGGTCA 
     4501 CACAGTGTAA TGCAAAATAT GTGGAATGTT TCAGTGCTCA GAAAGAGTGT AACAAAGAAA 
     4561 AGAACAGAAA CTCTTCAGTT GTGCCATCTG AGCGTGCTCG AGTGGGTCTT GCACCATTGC 
     4621 CTGGAATGAA AGGAACAGAT TACATTAATG CTTCTTATAT CATGGGCTAT TATAGGAGCA 
     4681 ATGAATTTAT TATAACTCAG CATCCTCTGC CACATACTAC GAAAGATTTC TGGCGAATGA 
     4741 TTTGGGATCA TAACGCACAG ATCATTGTCA TGCTGCCAGA CAACCAGAGC TTGGCAGAAG 
     4801 ATGAGTTTGT GTACTGGCCA AGTCGAGAAG AATCCATGAA CTGTGAGGCC TTTACCGTCA 
     4861 CCCTTATCAG CAAAGACAGA CTGTGCCTCT CTAATGAAGA ACAAATTATC ATCCATGACT 
     4921 TTATCCTTGA AGCTACACAG GATGACTATG TCTTAGAAGT TCGGCACTTT CAGTGTCCCA 
     4981 AATGGCCTAA CCCAGATGCC CCCATAAGTA GTACCTTTGA ACTTATCAAC GTCATCAAGG 
     5041 AAGAGGCCTT AACAAGGGAT GGTCCCACCA TTGTTCATGA TGAGTATGGA GCAGTTTCAG 
     5101 CAGGAATGTT ATGTGCCCTT ACCACCCTGT CCCAGCAACT GGAGAATGAA AATGCTGTGG 
     5161 ATGTTTTCCA GGTTGCAAAA ATGATCAATC TTATGAGGCC TGGAGTATTC ACAGACATTG 
     5221 AACAATACCA GTTCATCTAT AAAGCAAGGC TTAGCTTGGT CAGCACTAAA GAAAATGGAA 
     5281 ATGGTCCCAT GACAGTAGAC AAAAATGGTG CTGTTCTTAT TGCAGATGAA TCAGACCCTG 
     5341 CTGAGAGCAT GGAGTCCCTA GTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5401 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5461 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5521 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5581 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5641 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5701 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5761 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5821 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5881 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5941 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6001 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6061 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6121 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6181 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6241 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6301 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6361 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6421 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6481 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6541 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6601 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6661 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6721 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6781 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6841 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6901 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6961 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7021 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7081 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7141 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7201 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7261 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7321 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7381 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7441 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7501 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7561 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7621 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7681 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7741 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7801 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7861 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7921 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7981 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8041 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8101 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8161 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8221 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8281 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8341 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8401 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8461 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8521 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8581 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8641 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8701 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8761 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8821 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8881 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8941 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9001 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9061 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9121 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9181 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//